BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.1 - phase: 0
(141 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14309 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 54 1e-08
TC14307 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 53 2e-08
TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 53 2e-08
TC14311 homologue to UP|Q9FFC0 (Q9FFC0) Histone H2B like protein... 51 9e-08
TC14310 homologue to UP|Q9M3H6 (Q9M3H6) Histone H2B, partial (92%) 50 1e-07
AI967840 45 5e-06
TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , com... 30 0.12
AV415208 30 0.21
AU251825 29 0.35
BP053310 26 3.0
BP041645 26 3.0
TC14124 UP|Q8H945 (Q8H945) Phosphoenolpyruvate carboxylase , co... 25 5.1
AV767738 25 6.6
AW720308 24 8.6
TC8600 similar to UP|O80798 (O80798) T8F5.4 protein (Expressed p... 24 8.6
>TC14309 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (93%)
Length = 682
Score = 53.5 bits (127), Expect = 1e-08
Identities = 30/102 (29%), Positives = 59/102 (57%), Gaps = 6/102 (5%)
Frame = +1
Query: 4 KKGKNIILEVGSSSGTKRR-----TVE-FKKHIYRVAKKVHPDLDITVESIEMMNDIMND 57
K GK + E S++G K++ VE +K +I++V K+VHPD+ I+ +++ +MN +ND
Sbjct: 166 KAGKKLPKEGASAAGDKKKKRSKKNVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFIND 345
Query: 58 MLHKIVEEVAKNVAALNQKLLVERDLWVGVKELYPKYLARCA 99
+ K+ +E ++ + + R++ V+ + P LA+ A
Sbjct: 346 IFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHA 471
>TC14307 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (90%)
Length = 701
Score = 52.8 bits (125), Expect = 2e-08
Identities = 29/102 (28%), Positives = 60/102 (58%), Gaps = 6/102 (5%)
Frame = +2
Query: 4 KKGKNIILEVGSSSGTKRR-----TVE-FKKHIYRVAKKVHPDLDITVESIEMMNDIMND 57
K GK + E +++G K++ +VE +K +I++V K+VHPD+ I+ +++ +MN +ND
Sbjct: 164 KAGKKLPKEGAAAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFIND 343
Query: 58 MLHKIVEEVAKNVAALNQKLLVERDLWVGVKELYPKYLARCA 99
+ K+ +E ++ + + R++ V+ + P LA+ A
Sbjct: 344 IFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHA 469
>TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (96%)
Length = 764
Score = 52.8 bits (125), Expect = 2e-08
Identities = 29/102 (28%), Positives = 60/102 (58%), Gaps = 6/102 (5%)
Frame = +3
Query: 4 KKGKNIILEVGSSSGTKRR-----TVE-FKKHIYRVAKKVHPDLDITVESIEMMNDIMND 57
K GK + E +++G K++ +VE +K +I++V K+VHPD+ I+ +++ +MN +ND
Sbjct: 174 KAGKKLPKEGAAAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFIND 353
Query: 58 MLHKIVEEVAKNVAALNQKLLVERDLWVGVKELYPKYLARCA 99
+ K+ +E ++ + + R++ V+ + P LA+ A
Sbjct: 354 IFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHA 479
>TC14311 homologue to UP|Q9FFC0 (Q9FFC0) Histone H2B like protein, partial
(97%)
Length = 626
Score = 50.8 bits (120), Expect = 9e-08
Identities = 26/82 (31%), Positives = 51/82 (61%), Gaps = 1/82 (1%)
Frame = +1
Query: 19 TKRRTVEFKKHIYRVAKKVHPDLDITVESIEMMNDIMNDMLHKIVEEVAKNVAALNQK-L 77
+K+ +K +I++V K+VHPD+ I+ +++ +MN +ND+ K+ +E + +A NQK
Sbjct: 184 SKKNVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQE-SSRLARYNQKPT 360
Query: 78 LVERDLWVGVKELYPKYLARCA 99
+ R++ V+ + P LA+ A
Sbjct: 361 ITSREIQTAVRLVLPGELAQHA 426
>TC14310 homologue to UP|Q9M3H6 (Q9M3H6) Histone H2B, partial (92%)
Length = 502
Score = 50.1 bits (118), Expect = 1e-07
Identities = 30/99 (30%), Positives = 56/99 (56%), Gaps = 3/99 (3%)
Frame = +3
Query: 4 KKGKNIILEVGSSSGTKR--RTVE-FKKHIYRVAKKVHPDLDITVESIEMMNDIMNDMLH 60
K K I E GS K+ ++VE +K +I++V K+VHPD+ I+ +++ +MN +ND+
Sbjct: 135 KAEKKIAKEGGSEKKKKKSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFE 314
Query: 61 KIVEEVAKNVAALNQKLLVERDLWVGVKELYPKYLARCA 99
K+ +E ++ + + R++ V+ P LA+ A
Sbjct: 315 KLAQESSRLARYNKKPTITSREIQTAVRLGLPGELAKHA 431
>AI967840
Length = 393
Score = 45.1 bits (105), Expect = 5e-06
Identities = 20/70 (28%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Frame = +1
Query: 20 KRRTVE-FKKHIYRVAKKVHPDLDITVESIEMMNDIMNDMLHKIVEEVAKNVAALNQKLL 78
K ++VE +K +I++V K+VHPD+ I+ +++ +MN +ND+ K+ +E ++ + +
Sbjct: 175 KSKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTI 354
Query: 79 VERDLWVGVK 88
R++ V+
Sbjct: 355 TSREIQTAVR 384
>TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , complete
Length = 3376
Score = 30.4 bits (67), Expect = 0.12
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 57 DMLHKIVEEVAKNVAALNQKLLVERDLWVGVKELYPKY 94
D++ + + +AALN +LLV DLW ++L KY
Sbjct: 2564 DLVEMVFAKGDPGIAALNDRLLVSEDLWPFGEQLRNKY 2677
>AV415208
Length = 242
Score = 29.6 bits (65), Expect = 0.21
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 4/45 (8%)
Frame = +3
Query: 4 KKGKNIILEVGSSSGTKRRTVE----FKKHIYRVAKKVHPDLDIT 44
K K I + S K+RT + +K +I++V K+VHPD+ I+
Sbjct: 102 KAEKKISKDATGSEKKKKRTKKSVETYKIYIFKVLKQVHPDIGIS 236
>AU251825
Length = 237
Score = 28.9 bits (63), Expect = 0.35
Identities = 16/44 (36%), Positives = 28/44 (63%), Gaps = 6/44 (13%)
Frame = +3
Query: 7 KNIILEVGSSSGTKRR-----TVE-FKKHIYRVAKKVHPDLDIT 44
K I + G ++G K++ VE +K +I++V K+VHPD+ I+
Sbjct: 102 KKIPAKEGGAAGDKKKKRSKKNVETYKIYIFKVLKQVHPDIGIS 233
>BP053310
Length = 473
Score = 25.8 bits (55), Expect = 3.0
Identities = 13/31 (41%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
Frame = -2
Query: 52 NDIM-NDMLHKIVEEVAKNVAALNQKLLVER 81
N++M NDML +IVE K++A ++ + L E+
Sbjct: 466 NEVMENDMLKEIVEAHGKSIAQVSLRWLYEQ 374
>BP041645
Length = 508
Score = 25.8 bits (55), Expect = 3.0
Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = -1
Query: 46 ESIEMMNDIMNDMLHKIVEEVAKNVAALNQKLLVERDLWVG----VKELYPKYLARC 98
E I+ D + LHK E NVA++ ++L E+D W+ ++ ++L RC
Sbjct: 385 ERIQESLDRLTSELHKHEE----NVASVRRRLSYEKDKWLSSCPDTLKINMEFLQRC 227
>TC14124 UP|Q8H945 (Q8H945) Phosphoenolpyruvate carboxylase , complete
Length = 3367
Score = 25.0 bits (53), Expect = 5.1
Identities = 12/49 (24%), Positives = 24/49 (48%), Gaps = 9/49 (18%)
Frame = +2
Query: 45 VESIEMMNDIMN---------DMLHKIVEEVAKNVAALNQKLLVERDLW 84
+ ++ M+ ++ N D++ + + +AAL +LLV DLW
Sbjct: 2531 IRNLNMLQEMYNQWPFFRVTIDLVEMVFAKGDPGIAALYDRLLVSEDLW 2677
>AV767738
Length = 571
Score = 24.6 bits (52), Expect = 6.6
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = -3
Query: 38 HPDLDITVESIEMMNDIMNDMLHKIVEEVAK 68
H + D+ ES E +NDI DML+ + ++ +
Sbjct: 482 HQERDVFAES-ENVNDIQEDMLNDVYDKATE 393
>AW720308
Length = 492
Score = 24.3 bits (51), Expect = 8.6
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -3
Query: 101 LHAYFRRNFYETHRVGDLMARHAIPDS 127
+H YF + H ARHA+PD+
Sbjct: 103 VHKYFPHQYQCKHYCDIWKARHALPDT 23
>TC8600 similar to UP|O80798 (O80798) T8F5.4 protein (Expressed protein)
(At1g65270), partial (33%)
Length = 642
Score = 24.3 bits (51), Expect = 8.6
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = -3
Query: 17 SGTKRRTVEFKKHIYRVAKKVH 38
SG RT F +HI+R+ ++H
Sbjct: 295 SGATLRTSLFLRHIHRLGNRIH 230
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,924,419
Number of Sequences: 28460
Number of extensions: 20296
Number of successful extensions: 99
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of query: 141
length of database: 4,897,600
effective HSP length: 81
effective length of query: 60
effective length of database: 2,592,340
effective search space: 155540400
effective search space used: 155540400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148996.1