BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148995.13 - phase: 0
(160 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18221 111 5e-26
TC8503 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 an... 60 2e-13
TC18353 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 a... 44 1e-05
TC12542 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 a... 33 0.023
TC9150 similar to UP|Q9M6E4 (Q9M6E4) Poly(A)-binding protein (Fr... 28 0.75
AV428942 27 1.3
AV408845 27 2.2
TC10235 27 2.2
AV777131 27 2.2
AU251444 27 2.2
CB829244 26 2.9
TC15900 similar to UP|Q8GU54 (Q8GU54) SMC3 protein, partial (15%) 26 2.9
AV422226 26 3.7
AV407759 26 3.7
TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 26 3.7
TC10129 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (10%) 25 4.9
TC15003 weakly similar to UP|Q7X9I9 (Q7X9I9) Caffeic acid O-meth... 25 4.9
AV424522 25 4.9
AV776842 25 4.9
BP034655 25 6.4
>TC18221
Length = 590
Score = 111 bits (278), Expect = 5e-26
Identities = 56/94 (59%), Positives = 70/94 (73%), Gaps = 9/94 (9%)
Frame = +2
Query: 76 ASSSLPMNIPDWSKILKEEYKKKKESSD---DEDEGDYD------GVVQLPPHEYLARTR 126
A SSLP+N+PDWSKIL +EY++ + ++ DED+GD D G ++PPHE+LAR R
Sbjct: 62 APSSLPVNVPDWSKILGDEYRQSRRRNNHHHDEDDGDDDDGEEFHGGGRVPPHEFLARNR 241
Query: 127 GASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED 160
ASLSVHEG GRTLKGRDL VRNA+W K GF+D
Sbjct: 242 MASLSVHEGIGRTLKGRDLSRVRNAVWAKTGFQD 343
>TC8503 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 and
gb|T44635 come from this gene. {Arabidopsis thaliana;},
partial (26%)
Length = 1151
Score = 60.1 bits (144), Expect(2) = 2e-13
Identities = 33/57 (57%), Positives = 40/57 (69%), Gaps = 1/57 (1%)
Frame = +2
Query: 103 DDEDEGDYDGVVQLPPHEYLARTRG-ASLSVHEGKGRTLKGRDLRSVRNAIWKKVGF 158
DD DE + + V PPHEYLAR+R A+ SV G GRTLKGRDL VR+A+W + GF
Sbjct: 557 DDLDESELEVV---PPHEYLARSRKKAADSVFVGVGRTLKGRDLSRVRDAVWSQTGF 718
Score = 30.0 bits (66), Expect(2) = 2e-13
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3
Query: 77 SSSLPMNIPDWSKI 90
+SS PMN+PDWSKI
Sbjct: 474 ASSAPMNVPDWSKI 515
>TC18353 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 and
gb|T44635 come from this gene. {Arabidopsis thaliana;},
partial (15%)
Length = 656
Score = 44.3 bits (103), Expect = 1e-05
Identities = 20/32 (62%), Positives = 24/32 (74%)
Frame = +2
Query: 128 ASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFE 159
A+ SV EG GRTLKGRDL VR+A+W GF+
Sbjct: 5 AANSVFEGVGRTLKGRDLSRVRDAVWSPTGFD 100
>TC12542 similar to GB|AAD30253.1|4835787|F25C20 ESTs gb|R65381 and
gb|T44635 come from this gene. {Arabidopsis thaliana;},
partial (12%)
Length = 541
Score = 33.1 bits (74), Expect = 0.023
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = +3
Query: 77 SSSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPP 118
++S P+N+PDWSKIL+ K ES D + D D ++ P
Sbjct: 423 ATSAPVNVPDWSKILR---VKSVESLHDGFDDDGDNEPEMVP 539
>TC9150 similar to UP|Q9M6E4 (Q9M6E4) Poly(A)-binding protein (Fragment),
partial (65%)
Length = 1191
Score = 28.1 bits (61), Expect = 0.75
Identities = 20/69 (28%), Positives = 36/69 (51%)
Frame = +3
Query: 28 GKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMEANNKVNPLASSSLPMNIPDW 87
G LE D+ +V ++ S + + + L+ V+++ +ANN + LAS SL N+
Sbjct: 795 GMLLEMDQPEVLHLIESPDALKAKVAEALDVLRNVAQQ-QANNPADQLASLSLNDNLAS* 971
Query: 88 SKILKEEYK 96
++K E K
Sbjct: 972 ILLVKHESK 998
>AV428942
Length = 362
Score = 27.3 bits (59), Expect = 1.3
Identities = 13/35 (37%), Positives = 21/35 (59%)
Frame = +3
Query: 89 KILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLA 123
K+L+E+ K + DD+D+G +D + P E LA
Sbjct: 219 KLLEEDLKNDDLTIDDDDDGSFDDI----PEEELA 311
>AV408845
Length = 429
Score = 26.6 bits (57), Expect = 2.2
Identities = 9/26 (34%), Positives = 20/26 (76%)
Frame = -3
Query: 92 KEEYKKKKESSDDEDEGDYDGVVQLP 117
KEE ++++E ++E+EG++ G+ + P
Sbjct: 142 KEEEEEEEEEEEEEEEGEFVGLEKSP 65
>TC10235
Length = 1116
Score = 26.6 bits (57), Expect = 2.2
Identities = 25/84 (29%), Positives = 38/84 (44%), Gaps = 8/84 (9%)
Frame = +1
Query: 81 PMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPP-------HEYLARTRG-ASLSV 132
P++ P ++K EE KKK+++SD V QLPP E LA + A+ V
Sbjct: 703 PIHFPSFTK---EEEKKKQDNSDP--------VPQLPPLCIFHFSKEKLAELKAKANAEV 849
Query: 133 HEGKGRTLKGRDLRSVRNAIWKKV 156
G L+++ IW+ V
Sbjct: 850 DNGMHENRVISSLQALLTHIWRSV 921
>AV777131
Length = 408
Score = 26.6 bits (57), Expect = 2.2
Identities = 14/49 (28%), Positives = 20/49 (40%)
Frame = +2
Query: 63 SRKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYD 111
SRK + ++ + P+N + E K DEDEGD D
Sbjct: 194 SRKRRKDRSMSKKQDLTKPVNFVSTGTFMPNEEIDKNSREKDEDEGDGD 340
>AU251444
Length = 362
Score = 26.6 bits (57), Expect = 2.2
Identities = 15/42 (35%), Positives = 22/42 (51%)
Frame = +3
Query: 92 KEEYKKKKESSDDEDEGDYDGVVQLPPHEYLARTRGASLSVH 133
++EY DD+D+G+ G Q E+LA ASL+ H
Sbjct: 54 RQEYATDDYGEDDDDDGELYGNYQ-AEDEFLAEKLKASLNNH 176
>CB829244
Length = 478
Score = 26.2 bits (56), Expect = 2.9
Identities = 12/38 (31%), Positives = 23/38 (59%)
Frame = -3
Query: 121 YLARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGF 158
YL+R R +EG+ R K ++LR ++ ++W+ + F
Sbjct: 245 YLSRKR------NEGRKRRRKMKELRRMKRSVWRSLYF 150
>TC15900 similar to UP|Q8GU54 (Q8GU54) SMC3 protein, partial (15%)
Length = 881
Score = 26.2 bits (56), Expect = 2.9
Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +1
Query: 97 KKKESSDDEDEGDYDGVVQLPPHEYLARTRGASLSV-HEGKGRTLKGRDL 145
KKK+ D+D+ D DG + P + + G + V G+G T + L
Sbjct: 91 KKKDGDHDDDDQDEDGPREANPEGRVEKYIGVKVKVSFTGQGETQSMKQL 240
>AV422226
Length = 429
Score = 25.8 bits (55), Expect = 3.7
Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 5/47 (10%)
Frame = +3
Query: 58 GLKRVSRKMEANNKVNPLASSSLPMNIPDWSKIL-----KEEYKKKK 99
GLK +SR ++ N N L+SSS + +K+L KE+Y+K+K
Sbjct: 255 GLKSLSRNLQ*LNNKNLLSSSSRIILTSLKTKLLRLQNVKEKYQKQK 395
>AV407759
Length = 429
Score = 25.8 bits (55), Expect = 3.7
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 93 EEYKKKKESSDDEDEGDYDGVVQ 115
EE +++ S DE+EG +DGVVQ
Sbjct: 361 EEIPEQQSESSDEEEG-FDGVVQ 426
>TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (43%)
Length = 613
Score = 25.8 bits (55), Expect = 3.7
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 92 KEEYKKKKESSDDEDEGDYDGVVQLPPH 119
+ E + +E+ ++EDEGD G Q P H
Sbjct: 168 EREDTENEENENEEDEGDEQGEGQCP*H 85
>TC10129 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (10%)
Length = 562
Score = 25.4 bits (54), Expect = 4.9
Identities = 19/77 (24%), Positives = 32/77 (40%)
Frame = +3
Query: 3 SSRKSFLSRTSYIFPETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRV 62
SS SF ++S I+P T+ N + L + +SN +T+ P +
Sbjct: 132 SSESSFPKQSSSIYPNTSKNSFPNISTTLHSITS*---NPFSNFSTSTCPNTSKHSFPNI 302
Query: 63 SRKMEANNKVNPLASSS 79
S + +N NP + S
Sbjct: 303 STSLHSNTSKNPFSHFS 353
>TC15003 weakly similar to UP|Q7X9I9 (Q7X9I9) Caffeic acid
O-methyltransferase, partial (26%)
Length = 797
Score = 25.4 bits (54), Expect = 4.9
Identities = 9/26 (34%), Positives = 18/26 (68%)
Frame = +3
Query: 134 EGKGRTLKGRDLRSVRNAIWKKVGFE 159
+G+ + L+ R++R R+A+W GF+
Sbjct: 354 DGEKKDLQCREVREWRDALWLNTGFK 431
>AV424522
Length = 276
Score = 25.4 bits (54), Expect = 4.9
Identities = 16/43 (37%), Positives = 19/43 (43%)
Frame = +1
Query: 66 MEANNKVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDEDEG 108
+E N V P S+P IPD KK DDED+G
Sbjct: 139 IEDPNAVKPEGYDSIPAEIPD-------PKAKKPIDWDDEDDG 246
>AV776842
Length = 380
Score = 25.4 bits (54), Expect = 4.9
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = -3
Query: 57 PGLKRVSRKMEANNKVNPLASSSLPMN--IPDWSKILKEEYKKKKES 101
P + SRK K++P S+S+P N + K LK + +K KES
Sbjct: 378 PSINSFSRKTLTLFKLSPSTSNSIPNNEVTRRYFKTLKFQPEKGKES 238
>BP034655
Length = 517
Score = 25.0 bits (53), Expect = 6.4
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = +1
Query: 92 KEEYKKKKESSDDEDEGDYDGVVQLPPH 119
+EE +++++ DDE++ D D + P H
Sbjct: 196 EEEEEEEEDEEDDEEDEDEDYIPVAPSH 279
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.308 0.128 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,568,588
Number of Sequences: 28460
Number of extensions: 29688
Number of successful extensions: 189
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of query: 160
length of database: 4,897,600
effective HSP length: 83
effective length of query: 77
effective length of database: 2,535,420
effective search space: 195227340
effective search space used: 195227340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148995.13