BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148994.4 - phase: 0
(520 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV418028 207 3e-54
TC9223 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol 3-O-gluc... 150 5e-37
AV777407 120 5e-28
BP057349 57 1e-08
TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose glucosyl... 31 0.56
CB829041 30 0.96
BU494422 28 2.8
TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%) 28 2.8
AW428931 28 4.8
TC12152 weakly similar to UP|C7DA_SOYBN (O48923) Cytochrome P450... 28 4.8
TC8211 homologue to UP|PDX1_PHAVU (Q9FT25) Probable pyridoxin bi... 28 4.8
TC8600 similar to UP|O80798 (O80798) T8F5.4 protein (Expressed p... 28 4.8
BP072267 28 4.8
TC16316 homologue to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DN... 27 6.2
TC15121 27 8.1
>AV418028
Length = 366
Score = 207 bits (528), Expect = 3e-54
Identities = 93/120 (77%), Positives = 105/120 (87%)
Frame = +2
Query: 168 ESNEPFKADAIIANPPAYGHTHVAEYLNVPLHIFFTMPWTPTSDFPHPLSRVRQPIGYRL 227
+S FKADAIIANPPAYGHTHVAE L +P+HIFFTMPWTPT++FPHPLSRV+QP GYRL
Sbjct: 5 DSGVDFKADAIIANPPAYGHTHVAEALKIPIHIFFTMPWTPTAEFPHPLSRVKQPAGYRL 184
Query: 228 SYQIVDALIWLGIRDLINEFRKKKLKLRAVTYLRGSYTFPPDMPYGYIWSPHLVPKPKDW 287
SYQIVD+LIWLGIRD+IN+ RKK+LKLR VTYL GS D+P+ YIWSPHLVPKPKDW
Sbjct: 185 SYQIVDSLIWLGIRDMINDLRKKRLKLRPVTYLSGSQGSDTDIPHAYIWSPHLVPKPKDW 364
>TC9223 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol
3-O-glucosyltransferase, partial (16%)
Length = 659
Score = 150 bits (379), Expect = 5e-37
Identities = 70/111 (63%), Positives = 88/111 (79%)
Frame = +1
Query: 405 RAECPTTVVPFFGDQPFWGERVHARGVGPAPIRVEEFTLERLVDAIRFMLNPEVKKRAVE 464
+A CPTT+VPFFGDQPFWG+RVH RGVGP PI V+EF+L +LV+AI FML+P+VK+RA+E
Sbjct: 1 KAACPTTIVPFFGDQPFWGDRVHDRGVGPPPIPVDEFSLPKLVNAINFMLDPKVKERAIE 180
Query: 465 LANAMKNEDGVAGAVNAFYKHYPREKPDTEAEPRPVPSVHKHLSIRGCFGC 515
LA AM+NEDGV GAV AF+K P+ + TE + +P+PS SI CFGC
Sbjct: 181 LAKAMENEDGVTGAVKAFFKQLPQTRNKTEPDQQPLPS--SVFSISRCFGC 327
>AV777407
Length = 520
Score = 120 bits (301), Expect = 5e-28
Identities = 65/136 (47%), Positives = 78/136 (56%), Gaps = 30/136 (22%)
Frame = -2
Query: 200 IFFTMPWTPTSDFPHPLSRVRQPIGYR------------------------------LSY 229
+F P PT++FPHPLSRV+QP GYR LSY
Sbjct: 519 VFHIFPPRPTAEFPHPLSRVKQPAGYRVSSFI*PGSDLALNFVAFMCD*ILIIYNSQLSY 340
Query: 230 QIVDALIWLGIRDLINEFRKKKLKLRAVTYLRGSYTFPPDMPYGYIWSPHLVPKPKDWGP 289
QIVD+LIWLGIRD+IN+ RKK+LKLR VTYL GS D+P+ YIWSPHLVPKPK
Sbjct: 339 QIVDSLIWLGIRDMINDLRKKRLKLRPVTYLSGSQGSDTDIPHAYIWSPHLVPKPKG--- 169
Query: 290 NIDIVGFCFLDLASNY 305
CF L+S++
Sbjct: 168 -------CFRSLSSHF 142
>BP057349
Length = 493
Score = 56.6 bits (135), Expect = 1e-08
Identities = 33/95 (34%), Positives = 49/95 (50%), Gaps = 12/95 (12%)
Frame = -2
Query: 397 AGTTAAGLRAECPTTVVPFFGDQPFWGERVHARGVGPAPIRVEEFTLERLVD-------- 448
+GTTAA L+A P V PF DQ +W ER++ G+ P P+ ++ D
Sbjct: 453 SGTTAAALQAGTPQVVCPFMLDQFYWAERMYWLGISPEPLSRHHLLPDKNDDRSVQEAAH 274
Query: 449 ----AIRFMLNPEVKKRAVELANAMKNEDGVAGAV 479
AI L+ VK +A+E+A + EDGV+ A+
Sbjct: 273 VLSLAIHDALSSRVKTQAIEIAERLSLEDGVSEAI 169
>TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose
glucosyltransferase, partial (65%)
Length = 1585
Score = 30.8 bits (68), Expect = 0.56
Identities = 31/131 (23%), Positives = 50/131 (37%), Gaps = 11/131 (8%)
Frame = +1
Query: 301 LASN-YEPPKSLVDWLEEGE--NPIYVGFGSLPLQEPEKMTRII------VQALEQTGQR 351
L SN ++ + WLE E + +YV FGS+ + PE++ + +
Sbjct: 811 LGSNLWKEDTKCLQWLESKEPGSVVYVNFGSITVMSPEQLLEFAWGLANGKKPFLWIIRP 990
Query: 352 GIINKGWGGLGNLAELNTSKSVYLLDNCPHDWLF--PRCAAVVHHGGAGTTAAGLRAECP 409
++ G L TS + CP + + P + H G +T + A P
Sbjct: 991 DLVADGSAILSPKFVDETSNRGLIASWCPQEKVLNHPSVGGFLTHCGWNSTIESICAGVP 1170
Query: 410 TTVVPFFGDQP 420
PF DQP
Sbjct: 1171MLCWPFVADQP 1203
>CB829041
Length = 505
Score = 30.0 bits (66), Expect = 0.96
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2
Query: 10 QLRWLKNAFMVKDDGT 25
+LRWLK A +VKDDGT
Sbjct: 458 RLRWLKKASLVKDDGT 505
>BU494422
Length = 564
Score = 28.5 bits (62), Expect = 2.8
Identities = 16/48 (33%), Positives = 26/48 (53%)
Frame = -1
Query: 367 LNTSKSVYLLDNCPHDWLFPRCAAVVHHGGAGTTAAGLRAECPTTVVP 414
L+ + ++ L + P WL CAA+ HHG G+T + C +T+ P
Sbjct: 267 LSEASTLLLQPSFPSKWL-AFCAALQHHGPFGSTCS---CSCHSTLSP 136
>TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%)
Length = 799
Score = 28.5 bits (62), Expect = 2.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 160 PACNSRYPESNEPFKADAIIANPPA 184
P NS YP +N P A +I +PPA
Sbjct: 262 PTSNSPYPSTNPPSLAPSISTSPPA 336
>AW428931
Length = 284
Score = 27.7 bits (60), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (62%)
Frame = -3
Query: 408 CPTTVVPFFGDQPFWGERV 426
CPT ++ FF PFW R+
Sbjct: 222 CPTLILSFFSSCPFWPSRL 166
>TC12152 weakly similar to UP|C7DA_SOYBN (O48923) Cytochrome P450 71D10 ,
partial (13%)
Length = 429
Score = 27.7 bits (60), Expect = 4.8
Identities = 17/54 (31%), Positives = 27/54 (49%)
Frame = -2
Query: 132 KNKGFLPSGPSEIHLQRSQIRAIIHSLLPACNSRYPESNEPFKADAIIANPPAY 185
+ KG SG S + +++ ++H LLPA N RY P K + + N P +
Sbjct: 173 RGKGNWASGSSSVG*--GKVQGLVHILLPAANGRY-SGVVPLKVNWRMRNLPGF 21
>TC8211 homologue to UP|PDX1_PHAVU (Q9FT25) Probable pyridoxin biosynthesis
protein PDX1 (pvPDX1), partial (24%)
Length = 602
Score = 27.7 bits (60), Expect = 4.8
Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = +2
Query: 315 LEEGENPIYVGFGSLPLQEPEKMTRIIVQALEQTGQRGIINKGWGGLGN-LAELNTSKS 372
++ G + ++VG G +P K R IVQA+ G++ + GLG + LN + S
Sbjct: 20 MQLGCDGVFVGSGVFKSGDPAKRARAIVQAVTHYSDPGLLAEISCGLGEAMVGLNLNDS 196
>TC8600 similar to UP|O80798 (O80798) T8F5.4 protein (Expressed protein)
(At1g65270), partial (33%)
Length = 642
Score = 27.7 bits (60), Expect = 4.8
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -1
Query: 255 RAVTYLRGSYTFPPDMPYGYIWSPHLVPKPKDWGPNIDIVGFCFL 299
R + Y++ Y D+ G+I SP + P NI ++G C L
Sbjct: 216 RGIRYIQ--YLAQKDLSIGFIVSPSIPSPPSTSSANIGVLGACSL 88
>BP072267
Length = 373
Score = 27.7 bits (60), Expect = 4.8
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 352 GIINKGWGGLGNLAELNTSKSVYLL 376
G ++ WGG+G +AEL + VYLL
Sbjct: 186 GALSSDWGGMGQMAEL---RGVYLL 251
>TC16316 homologue to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DNA-binding
protein, partial (7%)
Length = 488
Score = 27.3 bits (59), Expect = 6.2
Identities = 20/51 (39%), Positives = 25/51 (48%)
Frame = -2
Query: 357 GWGGLGNLAELNTSKSVYLLDNCPHDWLFPRCAAVVHHGGAGTTAAGLRAE 407
GW G+ +LN +SV WL RCAAVVH GG G +R +
Sbjct: 475 GWTTXGSPHDLN-GRSV-----AGGVWLL-RCAAVVHGGGVGELEGVVRED 344
>TC15121
Length = 585
Score = 26.9 bits (58), Expect = 8.1
Identities = 14/37 (37%), Positives = 19/37 (50%)
Frame = +1
Query: 457 EVKKRAVELANAMKNEDGVAGAVNAFYKHYPREKPDT 493
E +++A+E M E A FYK YP + PDT
Sbjct: 43 EARRKAIE---EMSEETKAAFESMKFYKFYPVQSPDT 144
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.141 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,481,564
Number of Sequences: 28460
Number of extensions: 163268
Number of successful extensions: 734
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 733
length of query: 520
length of database: 4,897,600
effective HSP length: 94
effective length of query: 426
effective length of database: 2,222,360
effective search space: 946725360
effective search space used: 946725360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148994.4