BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148970.7 - phase: 0
(548 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19023 similar to UP|Q9M648 (Q9M648) FKF1 (ADAGIO 3), partial (... 185 1e-47
TC12771 similar to UP|Q945N7 (Q945N7) AT5g04420/T32M21_20, parti... 67 7e-12
AU089476 47 8e-06
CN825076 38 0.004
TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protei... 36 0.018
TC8573 similar to UP|Q9SIH5 (Q9SIH5) At2g36090 protein, partial ... 34 0.070
TC15606 weakly similar to GB|AAL15406.1|16323302|AY058232 At2g02... 33 0.091
BU494440 30 1.3
AV423739 29 1.7
TC17989 29 2.2
BP034385 29 2.2
TC9554 similar to UP|Q7XYW5 (Q7XYW5) Seed specific protein Bn15D... 28 2.9
CB828581 28 3.8
TC9586 similar to UP|Q93YZ5 (Q93YZ5) AT5g51550/K17N15_10, partia... 28 3.8
TC19722 similar to UP|O24147 (O24147) Kinesin-like protein, part... 27 6.5
TC16023 27 6.5
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 27 8.5
>TC19023 similar to UP|Q9M648 (Q9M648) FKF1 (ADAGIO 3), partial (19%)
Length = 716
Score = 185 bits (470), Expect = 1e-47
Identities = 81/128 (63%), Positives = 106/128 (82%)
Frame = +3
Query: 420 DVFTMDLSEDEPCWRCVTGSGMPGAGNPEGIAPPPRLDHVAVSLPGGRILIFGGSVAGLH 479
+ +T+DL +++P WR + + G + PPPRLDHVAVS+P GRI+IFGGS+AGLH
Sbjct: 6 EAYTIDLEDEKPQWRQLDCNEFSGLASQNPDVPPPRLDHVAVSMPCGRIIIFGGSIAGLH 185
Query: 480 SASQLYILDPTDEKPTWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSDLH 539
S SQL++LDP++EKP+WRILNVPG+PP+FAWGHSTCVVGGTR +VLGG TGEEW+L++LH
Sbjct: 186 SPSQLFLLDPSEEKPSWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHTGEEWVLNELH 365
Query: 540 ELSLANSV 547
EL LA+ +
Sbjct: 366 ELCLASDL 389
Score = 58.2 bits (139), Expect = 3e-09
Identities = 38/108 (35%), Positives = 53/108 (48%), Gaps = 11/108 (10%)
Frame = +3
Query: 311 DVFVLDLDATPPTWR-----EISGLAP-----PLPRSWHSSCTLDGTKLIVSGGCADSGV 360
+ + +DL+ P WR E SGLA P PR H + ++ ++I+ GG
Sbjct: 6 EAYTIDLEDEKPQWRQLDCNEFSGLASQNPDVPPPRLDHVAVSMPCGRIIIFGGSIAGLH 185
Query: 361 LLSDTFLLDMSMENPVWREIPVTWTPPS-RLGHTLSVYGGRKILMFGG 407
S FLLD S E P WR + V PP GH+ V GG ++L+ GG
Sbjct: 186 SPSQLFLLDPSEEKPSWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGG 329
Score = 53.1 bits (126), Expect = 1e-07
Identities = 30/93 (32%), Positives = 50/93 (53%), Gaps = 3/93 (3%)
Frame = +3
Query: 228 VEPSRCNFSACAVG-NRVVLFGGEGVNMQPMNDTFVLDLNSNNPEWQHVQV-SSPPPGRW 285
V P R + A ++ R+++FGG + + F+LD + P W+ + V PP W
Sbjct: 99 VPPPRLDHVAVSMPCGRIIIFGGSIAGLHSPSQLFLLDPSEEKPSWRILNVPGQPPKFAW 278
Query: 286 GHTLSCVNGSRLVVFGG-CGTQGLLNDVFVLDL 317
GH+ V G+R++V GG G + +LN++ L L
Sbjct: 279 GHSTCVVGGTRVLVLGGHTGEEWVLNELHELCL 377
Score = 44.7 bits (104), Expect = 4e-05
Identities = 31/120 (25%), Positives = 48/120 (39%), Gaps = 10/120 (8%)
Frame = +3
Query: 364 DTFLLDMSMENPVWREIPVTW----------TPPSRLGHTLSVYGGRKILMFGGLAKSGP 413
+ + +D+ E P WR++ PP RL H +I++FGG
Sbjct: 6 EAYTIDLEDEKPQWRQLDCNEFSGLASQNPDVPPPRLDHVAVSMPCGRIIIFGGSIAG-- 179
Query: 414 LRFRSSDVFTMDLSEDEPCWRCVTGSGMPGAGNPEGIAPPPRLDHVAVSLPGGRILIFGG 473
S +F +D SE++P WR + G P P H + G R+L+ GG
Sbjct: 180 -LHSPSQLFLLDPSEEKPSWRILNVPGQP---------PKFAWGHSTCVVGGTRVLVLGG 329
>TC12771 similar to UP|Q945N7 (Q945N7) AT5g04420/T32M21_20, partial (35%)
Length = 881
Score = 67.0 bits (162), Expect = 7e-12
Identities = 54/170 (31%), Positives = 78/170 (45%), Gaps = 10/170 (5%)
Frame = +2
Query: 248 GGEGVNMQPMNDTFVLDLNSNNPEWQHVQVSSPPPG-RWGHTLSCVNGSRLVVFGGCGTQ 306
GGE + + +ND VLDL S W ++ + PP R+ H + L+VFGGC
Sbjct: 5 GGEDMGRKLLNDVHVLDLESMT--WDMIKTTQTPPAPRYDHAAAMHGERYLLVFGGCSHS 178
Query: 307 GLLNDVFVLDLD----ATPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLL 362
ND+ +LDL + P T ++ PR+ H+ T+D + IV GG SG
Sbjct: 179 VFFNDLHLLDLQTMEWSQPQTQGDLVS-----PRAGHAGITIDESWFIVGGGDNKSG--C 337
Query: 363 SDTFLLDMSMENPVWREIPVTWTPPSRLGHTLSVY-----GGRKILMFGG 407
+T +LDMS VW + V LS+ G + +L FGG
Sbjct: 338 PETLVLDMS--KLVWSVLAVVKQKDPLSSEGLSICSAWIDGEKYLLAFGG 481
Score = 30.4 bits (67), Expect = 0.77
Identities = 20/72 (27%), Positives = 31/72 (42%)
Frame = +2
Query: 472 GGSVAGLHSASQLYILDPTDEKPTWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGE 531
GG G + +++LD E TW ++ PP + H+ + G +V GG
Sbjct: 5 GGEDMGRKLLNDVHVLDL--ESMTWDMIKTTQTPPAPRYDHAAAMHGERYLLVFGG-CSH 175
Query: 532 EWMLSDLHELSL 543
+DLH L L
Sbjct: 176 SVFFNDLHLLDL 211
>AU089476
Length = 548
Score = 47.0 bits (110), Expect = 8e-06
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +3
Query: 525 LGGQTGEEWMLSDLHELSLANSVI 548
LGGQTGEEWMLS+LHELSLA+S I
Sbjct: 9 LGGQTGEEWMLSELHELSLASSAI 80
>CN825076
Length = 663
Score = 38.1 bits (87), Expect = 0.004
Identities = 16/49 (32%), Positives = 26/49 (52%)
Frame = +3
Query: 148 ILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGSETTRVLETV 196
IL RL ++ASV+ C +++ E LW VC + W S ++T+
Sbjct: 408 ILRRLDGPELASVACTCAAFCSISKEESLWENVCSSVWPSTNREDVKTL 554
>TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protein, partial
(8%)
Length = 801
Score = 35.8 bits (81), Expect = 0.018
Identities = 23/67 (34%), Positives = 31/67 (45%), Gaps = 1/67 (1%)
Frame = +2
Query: 124 LGDRNVSRGICGIFQLSDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNE-DLWRMVCQ 182
L D N S F E + L IL+ L P +IA+ + R + N+ LW +C
Sbjct: 128 LNDPNSSSSSVVSFSDFPEDVQLCILSFLGPSEIATFACTSKRFLSLCTNDAKLWFTLCH 307
Query: 183 NAWGSET 189
WGSET
Sbjct: 308 RRWGSET 328
>TC8573 similar to UP|Q9SIH5 (Q9SIH5) At2g36090 protein, partial (17%)
Length = 515
Score = 33.9 bits (76), Expect = 0.070
Identities = 18/60 (30%), Positives = 26/60 (43%)
Frame = +2
Query: 128 NVSRGICGIFQLSDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGS 187
N + I L +++ IL RL +AS SS + L + LWR +C W S
Sbjct: 122 NSTNSATTITALHSDIIQSHILTRLDGPTLASTSSSTSHLRSLCTQNPLWREICTATWPS 301
>TC15606 weakly similar to GB|AAL15406.1|16323302|AY058232
At2g02710/T20F6.15 {Arabidopsis thaliana;}, partial
(25%)
Length = 642
Score = 33.5 bits (75), Expect = 0.091
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +1
Query: 35 ISEIRKCIDEGVEFQGELLNFRKDGSPLMNRLRLTPIY-GEDDEITHVIGIQL 86
+ IR+ + E E Q LN+RKDG+P L + P++ + H + +Q+
Sbjct: 484 VMAIREAVREEKEAQVVXLNYRKDGTPFXMLLLVCPVFSASSGGVVHFVAVQV 642
>BU494440
Length = 508
Score = 29.6 bits (65), Expect = 1.3
Identities = 10/47 (21%), Positives = 24/47 (50%)
Frame = +2
Query: 141 DEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGS 187
D+ L ++L + + +A V + ++ ++E LW ++C W +
Sbjct: 200 DDNLLFEVLKHVDAKSLAMAGCVNKQWHKTAQDERLWELICTKQWAN 340
>AV423739
Length = 465
Score = 29.3 bits (64), Expect = 1.7
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +1
Query: 148 ILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWG 186
IL RL P + ++ VC L E ++ LW + WG
Sbjct: 130 ILERLPPSALCQMAGVCRSLRESCVSDHLWERHMKQKWG 246
>TC17989
Length = 496
Score = 28.9 bits (63), Expect = 2.2
Identities = 15/48 (31%), Positives = 25/48 (51%)
Frame = +2
Query: 140 SDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGS 187
S+EVL + L D+AS SV + ++ R++ W+ +C W S
Sbjct: 176 SEEVLKA-VFPFLESVDLASCMSVSKQWNDIARDDFFWKCLCGKRWPS 316
>BP034385
Length = 481
Score = 28.9 bits (63), Expect = 2.2
Identities = 17/44 (38%), Positives = 21/44 (47%)
Frame = +2
Query: 135 GIFQLSDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWR 178
G F L IL L+ D+ S S+VCT L +E LWR
Sbjct: 347 GFFSKIPPELFHHILKFLSSEDLISCSAVCTFLNYAASDEALWR 478
>TC9554 similar to UP|Q7XYW5 (Q7XYW5) Seed specific protein Bn15D1B,
partial (13%)
Length = 750
Score = 28.5 bits (62), Expect = 2.9
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 321 PPTWREISGLAPPLPRSWHS 340
PP WR G PP+ R+W S
Sbjct: 408 PPFWRRRPGFVPPVWRTWRS 467
>CB828581
Length = 537
Score = 28.1 bits (61), Expect = 3.8
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = -1
Query: 494 PTWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGEEWM 534
P+W NV G R + G S C G R+ LGG+ G +WM
Sbjct: 147 PSWLPENV-GVGKRTSRGKSGCC--GNRSFFLGGEKGVKWM 34
>TC9586 similar to UP|Q93YZ5 (Q93YZ5) AT5g51550/K17N15_10, partial (52%)
Length = 738
Score = 28.1 bits (61), Expect = 3.8
Identities = 13/51 (25%), Positives = 21/51 (40%)
Frame = +2
Query: 492 EKPTWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSDLHELS 542
++ WR+L R F W C G T + +G G W + + L+
Sbjct: 293 QRTRWRMLGTRVRTRHFRWRSPICARGFTAPVEVGHTPGRCWTVEMVPRLT 445
>TC19722 similar to UP|O24147 (O24147) Kinesin-like protein, partial (23%)
Length = 875
Score = 27.3 bits (59), Expect = 6.5
Identities = 20/46 (43%), Positives = 25/46 (53%)
Frame = -1
Query: 105 SLKSSGRFHSVLSSLQPPPLGDRNVSRGICGIFQLSDEVLSLKILA 150
S+ S G F SVL S Q LG NVS C I L +++ L +LA
Sbjct: 467 SIASQGYFLSVLISEQKVHLGVLNVSNFAC-IVYLRAQLVHLSLLA 333
>TC16023
Length = 894
Score = 27.3 bits (59), Expect = 6.5
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +2
Query: 452 PPPRLDHVAVSLPGGRILIFGGSVAG 477
P PRL H + LP G ILI G+ G
Sbjct: 53 PEPRLLHDMLILPNGNILIINGAKHG 130
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 26.9 bits (58), Expect = 8.5
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = -2
Query: 200 KRLGWGRLARELTTLEAAAWRKLTVGGG 227
+R GW RL+ E+ + A AW GGG
Sbjct: 291 RRCGWSRLS*EVVVVCAVAWWWWWCGGG 208
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.139 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,881,124
Number of Sequences: 28460
Number of extensions: 189249
Number of successful extensions: 912
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 904
length of query: 548
length of database: 4,897,600
effective HSP length: 95
effective length of query: 453
effective length of database: 2,193,900
effective search space: 993836700
effective search space used: 993836700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148970.7