BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148969.6 + phase: 0
(280 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV428617 57 3e-09
TC12347 similar to GB|AAG33973.1|11320952|AF250959 peptide defor... 55 2e-08
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 32 0.12
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 32 0.16
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 31 0.21
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 31 0.27
TC8544 31 0.27
TC16026 similar to UP|Q9SGZ3 (Q9SGZ3) F28K19.29, partial (32%) 30 0.46
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 30 0.46
BP034655 30 0.46
BP043430 29 0.78
TC16467 UP|NUCC_LOTJA (Q9BBN8) NAD(P)H-quinone oxidoreductase ch... 29 1.0
TC12795 UP|Q9FMV5 (Q9FMV5) Transcription factor Hap5a-like prote... 29 1.0
TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, pa... 28 1.3
TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylas... 28 1.3
BP036655 28 1.7
TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratas... 28 1.7
TC17462 similar to PIR|A86383|A86383 76.4K protein kinase homolo... 28 2.3
BP030794 28 2.3
AV780096 28 2.3
>AV428617
Length = 355
Score = 57.0 bits (136), Expect = 3e-09
Identities = 35/69 (50%), Positives = 45/69 (64%), Gaps = 9/69 (13%)
Frame = +3
Query: 51 PPSNTKLSSFSSASSETA------FLSKTL---KSELPHIVQAGDPVLHEPAREVDHSEI 101
PP + LSS S A++ TA FL T K +LP V+AGDPVLHEPA+EV+ SEI
Sbjct: 147 PPLSLSLSSSS*AAAVTATARAGWFLGLTADNKKMKLPDTVKAGDPVLHEPAQEVNPSEI 326
Query: 102 NSDKIQKII 110
S+++ KII
Sbjct: 327 KSERVHKII 353
>TC12347 similar to GB|AAG33973.1|11320952|AF250959 peptide deformylase-like
protein {Arabidopsis thaliana;} , partial (14%)
Length = 517
Score = 54.7 bits (130), Expect = 2e-08
Identities = 22/31 (70%), Positives = 28/31 (89%)
Frame = +1
Query: 229 ECDHLDGTLYVDKMVPRTFRSWENINMSIAR 259
ECDHLDGTLYVDKMVPRTFR+ +N+N+ + +
Sbjct: 1 ECDHLDGTLYVDKMVPRTFRTVDNLNLPLGQ 93
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 32.0 bits (71), Expect = 0.12
Identities = 22/61 (36%), Positives = 37/61 (60%), Gaps = 2/61 (3%)
Frame = -3
Query: 21 LASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSS--ASSETAFLSKTLKSEL 78
L R + ++L+F + + SSSSSS ++ + S++ SS SS +SS ++ SK+L S
Sbjct: 921 LPGRALLPLLLLLFPSPSSSSSSSSSSSSSSSSSSSSSSSSSSPSSSSSSISSKSLCSAS 742
Query: 79 P 79
P
Sbjct: 741 P 739
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 31.6 bits (70), Expect = 0.16
Identities = 16/39 (41%), Positives = 28/39 (71%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKT 73
++A+ SSSSSS ++ + PS++ SS SS+S+ ++ S T
Sbjct: 320 ASASTSSSSSSSSSSSSPSSSSSSSSSSSSTSSSTSSST 204
Score = 28.9 bits (63), Expect = 1.0
Identities = 18/44 (40%), Positives = 28/44 (62%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSEL 78
S+++ SSSSSS ++ + S++ S+ SS SS T+ S T S L
Sbjct: 302 SSSSSSSSSSSPSSSSSSSSSSSSTSSSTSSSTSSYS*TSASNL 171
Score = 26.2 bits (56), Expect = 6.6
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = -3
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSETAFLSKT 73
SSSSSS + T S++ SS SS SS ++ S +
Sbjct: 338 SSSSSSASASTSSSSSSSSSSSSPSSSSSSSSSS 237
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 31.2 bits (69), Expect = 0.21
Identities = 19/48 (39%), Positives = 28/48 (57%)
Frame = -3
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELPHIVQAGDP 87
SSSSSS ++ +P S++ SS SS+SS ++ S + S L G P
Sbjct: 364 SSSSSSSSSSSPSSSSSSSSSSSSSSSSSSSSSSSSSSLSV*TGLGGP 221
Score = 29.3 bits (64), Expect = 0.78
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = -3
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
SSSSSS ++ + PS++ SS SS+SS ++ S + S
Sbjct: 367 SSSSSSSSSSSSPSSSSSSSSSSSSSSSSSSSSSSSS 257
Score = 27.7 bits (60), Expect = 2.3
Identities = 16/40 (40%), Positives = 27/40 (67%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTL 74
S+++ SSSSSS + + S++ SS SS+SS ++ S +L
Sbjct: 367 SSSSSSSSSSSSPSSSSSSSSSSSSSSSSSSSSSSSSSSL 248
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 30.8 bits (68), Expect = 0.27
Identities = 19/59 (32%), Positives = 32/59 (54%)
Frame = +1
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELPHIVQAGDPVLHEPA 93
S+++ SSSSSS ++ + PS++ S SS+SS ++ S + P + P PA
Sbjct: 277 SSSSSSSSSSSLSSSSTPSSSSSSPPSSSSSSSSSSSSSSSWSSPSSSSSSSPFSSGPA 453
>TC8544
Length = 1278
Score = 30.8 bits (68), Expect = 0.27
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Frame = -1
Query: 3 KLQGSSSLFETEMEAARRLASRLR-----VVPMMLVFSNATVSSSSSSCNTETPPSNTKL 57
KL+ +SS E E++ L ++ LV S T SSSS N+ +PPS + L
Sbjct: 312 KLRSTSSEEELELQLELELGVESMEK*SGLMRARLVASETTGSSSSLWINSHSPPSPSPL 133
Query: 58 SSFSSASSETA 68
SS + E A
Sbjct: 132 SSIDAKQREEA 100
>TC16026 similar to UP|Q9SGZ3 (Q9SGZ3) F28K19.29, partial (32%)
Length = 1183
Score = 30.0 bits (66), Expect = 0.46
Identities = 20/58 (34%), Positives = 29/58 (49%), Gaps = 6/58 (10%)
Frame = +2
Query: 34 FSNATVSSSSSSCNTETPPSNTKL------SSFSSASSETAFLSKTLKSELPHIVQAG 85
FS ++ SSSSSS + SN+ L +SF S+ S T F S + + H+ G
Sbjct: 632 FSPSSSSSSSSSSSPSPSSSNSSLPCSSPPNSFYSSPSVTPFFSDGCSTSMTHVFANG 805
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 30.0 bits (66), Expect = 0.46
Identities = 24/69 (34%), Positives = 34/69 (48%), Gaps = 10/69 (14%)
Frame = -2
Query: 6 GSSSLFETEMEAARRLASRLRVVPMMLVFSNATVSSSSSSCNT----------ETPPSNT 55
GS SL + AA + VP S+++ SSSSSS ++ TPPS++
Sbjct: 306 GSKSLDASSSSAAATVRRTRYSVPKSSSSSSSSSSSSSSSSSSSGSPPPPRPPSTPPSSS 127
Query: 56 KLSSFSSAS 64
SS SS+S
Sbjct: 126 SSSSSSSSS 100
Score = 27.3 bits (59), Expect = 3.0
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -2
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSET 67
SSSSSS ++ +PP SS SS SS +
Sbjct: 132 SSSSSSSSSSSPPPGASSSSSSSPSSSS 49
Score = 26.2 bits (56), Expect = 6.6
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = -2
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETA 68
S+++ SSSS S PPS SS SS+SS ++
Sbjct: 201 SSSSSSSSSGSPPPPRPPSTPPSSSSSSSSSSSS 100
>BP034655
Length = 517
Score = 30.0 bits (66), Expect = 0.46
Identities = 17/42 (40%), Positives = 30/42 (70%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
S+++ SSSSSS ++ + S++ SS SS+SS ++ S++ KS
Sbjct: 245 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSESPKS 120
Score = 30.0 bits (66), Expect = 0.46
Identities = 17/40 (42%), Positives = 26/40 (64%)
Frame = -3
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELP 79
SSSSSS ++ + S++ SS SS+SS ++ S + SE P
Sbjct: 245 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSESP 126
Score = 28.5 bits (62), Expect = 1.3
Identities = 16/42 (38%), Positives = 29/42 (68%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
S+++ SSSSSS ++ + S++ SS SS+SS ++ S + +S
Sbjct: 254 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSES 129
Score = 25.8 bits (55), Expect = 8.6
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -3
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETA 68
S+++ SSSSSS ++ + S++ SS SS S ++A
Sbjct: 218 SSSSSSSSSSSSSSSSSSSSSSSSSSSSESPKSA 117
>BP043430
Length = 493
Score = 29.3 bits (64), Expect = 0.78
Identities = 19/44 (43%), Positives = 27/44 (61%), Gaps = 2/44 (4%)
Frame = +3
Query: 40 SSSSSSCNTETPPSNTKLSSFSSASSET--AFLSKTLKSELPHI 81
SSSS + + P S++ LSS SS+SS T +F S +L LP +
Sbjct: 243 SSSSLNSSLSLPSSSSLLSSSSSSSSSTSLSFPSSSLSLPLPSL 374
>TC16467 UP|NUCC_LOTJA (Q9BBN8) NAD(P)H-quinone oxidoreductase chain H,
chloroplast (NAD(P)H dehydrogenase, chain H)
(NADH-plastoquinone oxidoreductase 49 kDa subunit) ,
complete
Length = 2407
Score = 28.9 bits (63), Expect = 1.0
Identities = 17/49 (34%), Positives = 30/49 (60%), Gaps = 1/49 (2%)
Frame = -3
Query: 125 LSAQKIGIPLRIIVL-EEPKENLYNYTEEVNKIIDRRPFDLLVILNPKL 172
L+ + + +PL I L + N +NY +V+ I+ R+P + LV++NP L
Sbjct: 860 LNNEPMNLPLFAIELPNQILHNTHNYQLDVDPIVFRKP-EALVLINPNL 717
>TC12795 UP|Q9FMV5 (Q9FMV5) Transcription factor Hap5a-like protein, partial
(40%)
Length = 405
Score = 28.9 bits (63), Expect = 1.0
Identities = 27/79 (34%), Positives = 40/79 (50%), Gaps = 4/79 (5%)
Frame = +3
Query: 29 PMMLVFSNATVSSSSSSCNTE-TPPSNTKLSSFSSASSETAFLS-KTLKSELPHIVQAGD 86
P L+ + T SSSS+S + + P+N K SS S+ S T+ LS + +S P + A
Sbjct: 78 PTPLLQPHFTTSSSSNSSSFRCSGPTNAKRSSTSTTSRTTSCLSLASRRS*RPTKMSA*S 257
Query: 87 PVLHEPA--REVDHSEINS 103
P H + R S +NS
Sbjct: 258 PPKHPSSSPRHASFSSLNS 314
>TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, partial (3%)
Length = 504
Score = 28.5 bits (62), Expect = 1.3
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 5/46 (10%)
Frame = -3
Query: 31 MLVFSNATVSSSSSS-----CNTETPPSNTKLSSFSSASSETAFLS 71
+L S++ +SSSSSS N PPS +K S SASS + L+
Sbjct: 343 LLADSSS*LSSSSSSRSPTISNKPVPPSKSKSSKMKSASSSSMSLA 206
>TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylase, complete
Length = 1917
Score = 28.5 bits (62), Expect = 1.3
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = +1
Query: 37 ATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELP 79
A +SSS+ C++ +PPS + SS S+S S ++ P
Sbjct: 106 AAATSSSTPCSSSSPPSPSPSSSSPSSSPNRRKTSPQFSADFP 234
>BP036655
Length = 556
Score = 28.1 bits (61), Expect = 1.7
Identities = 22/76 (28%), Positives = 38/76 (49%), Gaps = 3/76 (3%)
Frame = +1
Query: 7 SSSLFETEMEAARRLASRLRVVPMMLVFSNATVSSS---SSSCNTETPPSNTKLSSFSSA 63
SSS F T ++ + + + + +++++SSS SSS ++ T S++ +S SS
Sbjct: 208 SSSTFSTPHSSSSSSSFSVSISVSLSSSTSSSLSSSLISSSSLHSSTTSSSSSTTSSSSP 387
Query: 64 SSETAFLSKTLKSELP 79
SSE S LP
Sbjct: 388 SSERPSSSNRCSLSLP 435
>TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratase-like
protein, partial (29%)
Length = 731
Score = 28.1 bits (61), Expect = 1.7
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = +1
Query: 38 TVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELP 79
+VSSSSSS ++ PPS++ +++ T L+ T S LP
Sbjct: 328 SVSSSSSSESSSAPPSSSSNPLSPASALTTPHLTPTPSSPLP 453
>TC17462 similar to PIR|A86383|A86383 76.4K protein kinase homolog F4F7.29 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(14%)
Length = 549
Score = 27.7 bits (60), Expect = 2.3
Identities = 20/69 (28%), Positives = 33/69 (46%)
Frame = -1
Query: 21 LASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELPH 80
L S L + + +F A SSS S+ ++ SNT S F S+ ++++ SE+P
Sbjct: 345 LFSGLLLTQAIAIFKTAITSSSLSASSSAKYGSNTSESGFLSSMQN*IHCTRSI-SEIPT 169
Query: 81 IVQAGDPVL 89
PV+
Sbjct: 168 WTTGNRPVI 142
>BP030794
Length = 513
Score = 27.7 bits (60), Expect = 2.3
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = -2
Query: 20 RLASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSS----ASSETAFLSKTL 74
RL+S + ++P FS++ +SSS S + + SFSS +SS AF+S +
Sbjct: 371 RLSSIVLILPSTDSFSSSNISSSWLSS*ISSTKARNSFRSFSSSGRRSSSSNAFVSSCI 195
>AV780096
Length = 564
Score = 27.7 bits (60), Expect = 2.3
Identities = 18/40 (45%), Positives = 26/40 (65%)
Frame = -2
Query: 37 ATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
AT SSSSSS + + S++ SS SS+ SE L+ +L+S
Sbjct: 134 ATASSSSSSSSIWS*TSSSCCSSSSSSESEPFRLAMSLRS 15
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,302,556
Number of Sequences: 28460
Number of extensions: 58644
Number of successful extensions: 759
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 668
length of query: 280
length of database: 4,897,600
effective HSP length: 89
effective length of query: 191
effective length of database: 2,364,660
effective search space: 451650060
effective search space used: 451650060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148969.6