BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148969.3 + phase: 2 /pseudo
(595 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV774902 220 4e-58
BI420067 110 8e-25
BP039700 101 4e-22
TC19829 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {A... 97 6e-21
TC17136 weakly similar to UP|AAR18373 (AAR18373) Nucleobase-asco... 94 8e-20
TC18124 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {A... 88 4e-18
TC10527 76 2e-14
TC14907 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {A... 75 4e-14
TC18156 73 1e-13
TC15289 weakly similar to UP|AAR18373 (AAR18373) Nucleobase-asco... 70 1e-12
TC12599 similar to AAR18374 (AAR18374) Nucleobase-ascorbate tran... 66 2e-11
BP056642 51 6e-07
BP052176 47 1e-05
BP054886 44 6e-05
TC9110 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {Ar... 44 6e-05
BP052070 43 2e-04
BG662162 37 0.009
BP066916 33 0.17
TC10182 similar to UP|O63832 (O63832) Cytochrome c oxidase subun... 30 0.85
TC16112 similar to GB|AAL87371.1|19548015|AY081718 AT4g33920/F17... 30 1.5
>AV774902
Length = 594
Score = 220 bits (561), Expect = 4e-58
Identities = 104/179 (58%), Positives = 135/179 (75%)
Frame = +2
Query: 316 WFRFPYPLQWGTPVFNWKMAIVMCVVSLISSVDSVGTYHTSSLLAASGPPTPGVLSRGIG 375
W RFPYP QWG P+F++K +I+M +VSL++SVDSVGTY T+S PPTP V+SRG+
Sbjct: 41 WVRFPYP*QWGIPIFHFKTSIIMVIVSLVASVDSVGTYRTASQQVDLRPPTPRVVSRGMA 220
Query: 376 LEGFSSLLAGLWGTGMGSTTLTENVHTIAGTKMGSRRPVQLGACLLIVLSLFGKVGGFIA 435
LEGF S+ AGLWG+G GSTTLTEN+HTI TK+ SR+ V LGA LI+ S GKVG +A
Sbjct: 221 LEGFCSIWAGLWGSGTGSTTLTENIHTINSTKVASRKAVVLGAAFLILFS*IGKVGALLA 400
Query: 436 SIPEAMVAGLLCIMWAMLTALGLSNLRYTETGSSRNIIIVGLSLFFSLSIPAYFQQYES 494
SIP ++ A +LC MWA+ LGLS L+Y ++ S RN+ IVG+SLF +SIP+Y QQY++
Sbjct: 401 SIPLSLAASVLCFMWALTVGLGLSTLQYGQSASFRNMTIVGVSLFLGMSIPSYIQQYQA 577
>BI420067
Length = 499
Score = 110 bits (274), Expect = 8e-25
Identities = 56/159 (35%), Positives = 86/159 (53%), Gaps = 8/159 (5%)
Frame = +1
Query: 257 LLFFVLYLRKISVFGHHIFQIYAVPLGLAVTWTFAFLLTENGRMKH--------CQVNTS 308
L+F Y+ G H+F +AV + +A+ W +A++LT G H C+ + +
Sbjct: 22 LVFVSQYVPHALHSGKHVFDRFAVLITIAIVWLYAYILTVGGAYNHAARKTQTTCRTDRA 201
Query: 309 DTMTSPPWFRFPYPLQWGTPVFNWKMAIVMCVVSLISSVDSVGTYHTSSLLAASGPPTPG 368
+ + PW R PYP QWG P F+ A M + S ++ V+S G + A++ P P
Sbjct: 202 GLIDAAPWIRVPYPFQWGAPSFDAGEAFAMMMASFVALVESSGAFTAVYRFASATPLPPS 381
Query: 369 VLSRGIGLEGFSSLLAGLWGTGMGSTTLTENVHTIAGTK 407
+LSRGIG +G LL+GL+GTG GS+ EN +A T+
Sbjct: 382 ILSRGIGWQGVGILLSGLFGTGNGSSVSVENAGLLALTR 498
>BP039700
Length = 506
Score = 101 bits (251), Expect = 4e-22
Identities = 53/151 (35%), Positives = 87/151 (57%), Gaps = 2/151 (1%)
Frame = +2
Query: 72 PVMVDHDDLVLRRRPSPLNYELTDSPALVFLAVYGIQHYLSIIGSLILTPLVIAPAMGAS 131
P+M D L + + L Y L +P + Q+Y+ ++G+ ++ P +I PAMG S
Sbjct: 53 PLMADITHLPMEQLQD-LEYCLDSNPPWAETVLLAFQNYILMLGTSVMIPSLIVPAMGGS 229
Query: 132 HDETAAMVCTVLLVSGVTTLLHTIFGSRLPLIQGPSFVYLAPVLAIINSPEFQELNE--N 189
+ A ++ T+L VSG+ TLL +FG+RLP + G SF Y+ PV II Q++ +
Sbjct: 230 DGDKARVIQTLLFVSGINTLLQALFGTRLPAVVGGSFAYVVPVAYIITDSSLQKITDPHE 409
Query: 190 KFKHIMKELQGAIIIGSAFQTLLGYTGLMSL 220
+F M+ +QGA+I+ S+ Q +LGY+ + L
Sbjct: 410 RFIQTMRAIQGALIVASSIQIILGYSQVWGL 502
>TC19829 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {Arabidopsis
thaliana;} , partial (37%)
Length = 600
Score = 97.4 bits (241), Expect = 6e-21
Identities = 53/178 (29%), Positives = 90/178 (49%), Gaps = 14/178 (7%)
Frame = +3
Query: 190 KFKHIMKELQGAIIIGSAFQTLLGYTGLMSLLVRFINPVVVSSTIAAVGL------FL*L 243
KFK IM+ +QGA+I+ S Q +LG++GL + RF++P+ ++ VG F +
Sbjct: 42 KFKKIMRAIQGALIVASTLQIVLGFSGLWRNVARFLSPLSAVPLVSLVGFGLYELGFPGV 221
Query: 244 ARVLRSVQYRY*CLLFFVLYLRKISVFGHHIFQIYAVPLGLAVTWTFAFLLTENGRMKH- 302
A+ + ++F Y+ + G HIF +AV + + W +A LLT G +
Sbjct: 222 AKCIEIGLPELVLIVFVSQYVPHVLHKGKHIFDRFAVIFTIVIVWIYAHLLTVGGAYNNA 401
Query: 303 -------CQVNTSDTMTSPPWFRFPYPLQWGTPVFNWKMAIVMCVVSLISSVDSVGTY 353
C+ + + + + PW R PYP QWG P F+ A M + + ++ V+S G +
Sbjct: 402 AHKTQISCRTDRAGLIDNAPWIRVPYPFQWGAPSFDAGEAFAMMMAAFVALVESTGAF 575
>TC17136 weakly similar to UP|AAR18373 (AAR18373) Nucleobase-ascorbate
transporter 11, partial (11%)
Length = 512
Score = 93.6 bits (231), Expect = 8e-20
Identities = 43/90 (47%), Positives = 57/90 (62%)
Frame = +3
Query: 503 PSYFQPYIVTSHGPFRSKYEELNYVLNMIFSLHMVIAFLVALILDNTVPGSKQERELYGW 562
PSY PY S GP S + L++ +N + S++MV+ LVA +LDNTVPGS++ER +Y W
Sbjct: 3 PSYLIPYAAASSGPSHSGIKPLDFAINALLSMNMVVTLLVAFLLDNTVPGSQEERGVYTW 182
Query: 563 SKPNDAREDPFIVSEYGLPARVGRCFRWVK 592
SK D DP + SEY LP +V C W K
Sbjct: 183 SKAEDITADPSLPSEYSLPNQVACCCCWFK 272
>TC18124 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {Arabidopsis
thaliana;} , partial (26%)
Length = 836
Score = 87.8 bits (216), Expect = 4e-18
Identities = 44/124 (35%), Positives = 76/124 (60%), Gaps = 3/124 (2%)
Frame = +1
Query: 89 LNYELTDSPALV-FLAVYGIQHYLSIIGSLILTPLVIAPAMGASHDETAAMVCTVLLVSG 147
++Y +T P++ + G QHYL ++G+ +L P + P MG +DE A ++ T+L V+G
Sbjct: 463 VSYCITSPPSMA*GHSCLGFQHYLVMLGTTVLIPTALVPQMGGGNDEKAKVIQTLLFVAG 642
Query: 148 VTTLLHTIFGSRLPLIQGPSFVYLAPVLAIINSPEFQELNE--NKFKHIMKELQGAIIIG 205
+ T L ++FG+RLP + G S+ Y+ ++II + F + + KFK I++ QGA+I+
Sbjct: 643 INT*LQSMFGTRLPAVMGGSYTYVPTTISIILAGRFSDEPDPIEKFKRIVRASQGALIVA 822
Query: 206 SAFQ 209
S Q
Sbjct: 823 STLQ 834
>TC10527
Length = 529
Score = 75.9 bits (185), Expect = 2e-14
Identities = 36/116 (31%), Positives = 69/116 (59%), Gaps = 2/116 (1%)
Frame = +2
Query: 89 LNYELTDSPALVFLAVYGIQHYLSIIGSLILTPLVIAPAMGASHDETAAMVCTVLLVSGV 148
L+Y + +P+ + G QHY+ ++G+ +L + MG +H + A ++ T+L++S +
Sbjct: 182 LDYCIHSNPSWPEALLLGFQHYIVMLGTTVLIATSLVNQMGGTHGDKARVIQTMLIMSAI 361
Query: 149 TTLLHTIFGSRLPLIQGPSFVYLAPVLAIIN--SPEFQELNENKFKHIMKELQGAI 202
TLL T FGSRLP + G S Y+ PV+++IN + + +F + ++ +QG++
Sbjct: 362 NTLLQTWFGSRLPTVMGGSVAYILPVMSVINDYTDKTFSSEHERFTYTIRTVQGSL 529
>TC14907 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {Arabidopsis
thaliana;} , partial (28%)
Length = 734
Score = 74.7 bits (182), Expect = 4e-14
Identities = 47/164 (28%), Positives = 74/164 (44%), Gaps = 4/164 (2%)
Frame = +3
Query: 429 KVGGFIASIPEAMVAGLLCIMWAMLTALGLSNLRYTETGSSRNIIIVGLSLFFSLSIPAY 488
K G ASIP M+A L C+ + + A GLS L++ S R ++G S+F LSIP Y
Sbjct: 3 KFGAVFASIPPPMIAALYCLFFGYVGAGGLSFLQFCNLNSFRTKFVLGFSIFLGLSIPQY 182
Query: 489 FQQYESSPESNFSVPSYFQPYIVTSHGPFRSKYEELNYVLNMIFSLHMVIAFLVALILDN 548
F +Y + + +GP + N ++N+ F +A +VA LDN
Sbjct: 183 FNEYTA----------------INGYGPVHTGARWFNDIVNVPFQSKAFVAGVVAYFLDN 314
Query: 549 TV----PGSKQERELYGWSKPNDAREDPFIVSEYGLPARVGRCF 588
T+ +++R + W K + D Y LP + + F
Sbjct: 315 TLHKKESAIRKDRGKHWWDKYRSFKTDTRSEEFYSLPFNLNKYF 446
>TC18156
Length = 531
Score = 73.2 bits (178), Expect = 1e-13
Identities = 51/160 (31%), Positives = 77/160 (47%), Gaps = 6/160 (3%)
Frame = +1
Query: 436 SIPEAMVAGLLCIMWAMLTALGLSNLRYTETGSSRNIIIVGLSLFFSLSIPAYFQQYESS 495
SIP ++ A + C+++ ++ A+G+S ++++ S NI I+GLSLF +SIP YF S
Sbjct: 1 SIPLSIFAAIYCVLFGIVAAIGISFIQFSYYDSMSNIYILGLSLFLGISIPLYFIM-NIS 177
Query: 496 PESNFSVPSYFQPYIVTSHGPFRSKYEELNYVLNMIFSLHMVIAFLVALILDNT--VPGS 553
P+ GP R+ N +LN IFS +A +V +LDNT V
Sbjct: 178 PD---------------GRGPVRTNGGWFNDILNTIFSSPPTVAIIVGTVLDNTLDVKHK 312
Query: 554 KQERELYGW----SKPNDAREDPFIVSEYGLPARVGRCFR 589
+ +R L W + D R D F GL + FR
Sbjct: 313 EDDRGLTWWRPFQHRKGDVRNDEFYRFPLGLTEYIPSRFR 432
>TC15289 weakly similar to UP|AAR18373 (AAR18373) Nucleobase-ascorbate
transporter 11, partial (9%)
Length = 583
Score = 69.7 bits (169), Expect = 1e-12
Identities = 32/59 (54%), Positives = 44/59 (74%)
Frame = +2
Query: 128 MGASHDETAAMVCTVLLVSGVTTLLHTIFGSRLPLIQGPSFVYLAPVLAIINSPEFQEL 186
MG + TA ++ T+L +SG+TT+LH+ FG+RLPL+QG SFVYL P L IIN+ E+ L
Sbjct: 2 MGGTDTNTANVIPTMLFLSGITTILHSYFGTRLPLVQGGSFVYLEPALVIINAQEYCNL 178
>TC12599 similar to AAR18374 (AAR18374) Nucleobase-ascorbate transporter 12,
partial (6%)
Length = 527
Score = 65.9 bits (159), Expect = 2e-11
Identities = 27/44 (61%), Positives = 35/44 (79%)
Frame = +2
Query: 552 GSKQERELYGWSKPNDAREDPFIVSEYGLPARVGRCFRWVKWVG 595
GSKQER +Y WS+P+ AR +P + ++Y LP RVG+ FRWVKWVG
Sbjct: 2 GSKQERGVYVWSEPDVARREPAVANDYELPLRVGKIFRWVKWVG 133
>BP056642
Length = 495
Score = 50.8 bits (120), Expect = 6e-07
Identities = 23/54 (42%), Positives = 38/54 (69%)
Frame = +1
Query: 132 HDETAAMVCTVLLVSGVTTLLHTIFGSRLPLIQGPSFVYLAPVLAIINSPEFQE 185
++E A ++ T+L V+G+ TLL T+FGSRLP + G S+ Y+A ++II + F +
Sbjct: 19 NNEKARVIETLLXVAGINTLLQTLFGSRLPAVIGGSYTYVATTISIILAGRFSD 180
>BP052176
Length = 529
Score = 46.6 bits (109), Expect = 1e-05
Identities = 33/134 (24%), Positives = 58/134 (42%), Gaps = 4/134 (2%)
Frame = -2
Query: 459 SNLRYTETGSSRNIIIVGLSLFFSLSIPAYFQQYESSPESNFSVPSYFQPYIVTSHGPFR 518
S L++ S R I+G S+F SIP YF++Y + + +GP
Sbjct: 528 SFLQFCNLNSFRTKFILGFSIFMGFSIPQYFKEYTA----------------LKHYGPVH 397
Query: 519 SKYEELNYVLNMIFSLHMVIAFLVALILDNTV----PGSKQERELYGWSKPNDAREDPFI 574
+ N ++N+ FS +A +AL LD T+ ++++R + W + + D
Sbjct: 396 THARWFNDMINVPFSSEAFVAGSLALFLDATLRKNDSQTRKDRGAHWWDRFRSYKTDTRS 217
Query: 575 VSEYGLPARVGRCF 588
Y LP + + F
Sbjct: 216 EEFYSLPFNLNKFF 175
>BP054886
Length = 601
Score = 44.3 bits (103), Expect = 6e-05
Identities = 48/132 (36%), Positives = 54/132 (40%), Gaps = 37/132 (28%)
Frame = +3
Query: 1 WAQKTGFKPVFSAETKA---GQSLSRQ---------PDLEASPVVSTPSQAVNDVPHGDK 48
WA+KTGF+P FS ET A GQ Q PDLE V P N V G K
Sbjct: 183 WAKKTGFRPKFSGETNAAHSGQITGPQPKPRESQPVPDLEMGRVRPPP----NGVAQGQK 350
Query: 49 VP----------PSPSDGVPTNN-------ARVEE-----RTTRLPVMVDH---DDLVLR 83
VP SDGVP+ N A VEE RT R +V+ DD
Sbjct: 351 VPVPAKDQSVKKRRDSDGVPSTNGQATPAAAVVEEPSQPRRTARHEELVEGLTVDDEGFA 530
Query: 84 RRPSPLNYELTD 95
R + YEL D
Sbjct: 531 SRHLHMKYELRD 566
>TC9110 similar to GB|BAB10858.1|10177467|AB009053 permease 1 {Arabidopsis
thaliana;} , partial (20%)
Length = 595
Score = 44.3 bits (103), Expect = 6e-05
Identities = 30/119 (25%), Positives = 49/119 (40%), Gaps = 4/119 (3%)
Frame = +1
Query: 474 IVGLSLFFSLSIPAYFQQYESSPESNFSVPSYFQPYIVTSHGPFRSKYEELNYVLNMIFS 533
I+G S+F LSIP YF +Y + + GP + N ++N+ F
Sbjct: 7 ILGFSIFLGLSIPQYFNEYTA----------------INGFGPVHTGARWFNDIINVPFQ 138
Query: 534 LHMVIAFLVALILDNTV----PGSKQERELYGWSKPNDAREDPFIVSEYGLPARVGRCF 588
+ +VA LDNT+ +++R + W K + D Y LP + + F
Sbjct: 139 SKPFVGGVVAYFLDNTLYKRDSAIRKDRGKHWWDKYKSFKGDTRSEEFYSLPFNLNKYF 315
>BP052070
Length = 480
Score = 42.7 bits (99), Expect = 2e-04
Identities = 29/115 (25%), Positives = 50/115 (43%), Gaps = 4/115 (3%)
Frame = -2
Query: 478 SLFFSLSIPAYFQQYESSPESNFSVPSYFQPYIVTSHGPFRSKYEELNYVLNMIFSLHMV 537
SLF LS+P YF + +++ HGP + N ++ +IFS
Sbjct: 479 SLFLGLSVPQYFN----------------EQLLLSGHGPVHTGSIAFNNIVQVIFSSPAT 348
Query: 538 IAFLVALILDNTV----PGSKQERELYGWSKPNDAREDPFIVSEYGLPARVGRCF 588
+A +VA +LD T+ ++++ + W K + +D Y LP + R F
Sbjct: 347 VAIIVAYLLDVTLSRGHSSTRRDSGRHWWEKFRNFDQDHRNEEFYSLPMNLNRYF 183
>BG662162
Length = 204
Score = 37.0 bits (84), Expect = 0.009
Identities = 16/43 (37%), Positives = 28/43 (64%)
Frame = +3
Query: 199 QGAIIIGSAFQTLLGYTGLMSLLVRFINPVVVSSTIAAVGLFL 241
+GA+I+ S Q +LG++GL + RF++P+ V ++ VG L
Sbjct: 12 EGALIVASTLQIVLGFSGLWRNVARFLSPLSVVPLVSLVGFGL 140
>BP066916
Length = 529
Score = 32.7 bits (73), Expect = 0.17
Identities = 21/59 (35%), Positives = 29/59 (48%), Gaps = 17/59 (28%)
Frame = -3
Query: 110 YLSIIGSLILTPLVIAPAMG---ASHDE--------------TAAMVCTVLLVSGVTTL 151
YLS+ GSL+L PLV+ P MG S +E +A +C V+ G+ TL
Sbjct: 527 YLSLXGSLVLIPLVMVPVMGGTDGSQEEVADTEQAENWFSLASAQRICVVVARGGMETL 351
>TC10182 similar to UP|O63832 (O63832) Cytochrome c oxidase subunit I
(Cytochrome c oxidase polypeptide I) (Fragment) ,
partial (10%)
Length = 879
Score = 30.4 bits (67), Expect = 0.85
Identities = 23/79 (29%), Positives = 32/79 (40%), Gaps = 24/79 (30%)
Frame = +3
Query: 2 AQKTGFKPV------------FSAETKAGQSLSRQP----------DLEASPVVSTPSQA 39
AQ+TG+ P + + G L R P DL++ V P A
Sbjct: 333 AQRTGYDPQRGQGYDPQRGQGYDPQIGQGYDLQRGPAYDPSRAAGYDLQSRGVAGPPGHA 512
Query: 40 --VNDVPHGDKVPPSPSDG 56
VN++P+G PPS S G
Sbjct: 513 PPVNNMPYGSSTPPSHSGG 569
>TC16112 similar to GB|AAL87371.1|19548015|AY081718 AT4g33920/F17I5_110
{Arabidopsis thaliana;}, partial (21%)
Length = 612
Score = 29.6 bits (65), Expect = 1.5
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -2
Query: 473 IIVGLSLFFSLSIPAYFQQYESSPESNFSVPSY 505
+I L LF IP +QQY SSP SNF +PSY
Sbjct: 473 VISNLCLFSR--IPFIYQQYSSSPISNF-LPSY 384
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,767,089
Number of Sequences: 28460
Number of extensions: 162854
Number of successful extensions: 1107
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1094
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1099
length of query: 595
length of database: 4,897,600
effective HSP length: 95
effective length of query: 500
effective length of database: 2,193,900
effective search space: 1096950000
effective search space used: 1096950000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148969.3