BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148918.3 + phase: 0
(1351 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 130 4e-37
BP066094 139 7e-36
AV780009 144 7e-35
AV427921 113 2e-25
BP084332 106 2e-23
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 89 5e-18
BP075943 87 2e-17
BP057233 81 1e-15
BP063694 74 2e-13
AU088980 70 2e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 70 2e-12
BP043850 69 6e-12
AV424544 68 1e-11
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 66 4e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 43 1e-10
AV766088 62 5e-10
BP052632 62 5e-10
TC9278 59 7e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 53 3e-07
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 52 5e-07
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 130 bits (327), Expect(2) = 4e-37
Identities = 81/148 (54%), Positives = 95/148 (63%), Gaps = 6/148 (4%)
Frame = -1
Query: 1090 QKKYASDILKKFKMEHSKPISTPVEEK-LKLTRESDGKRVDSTHYKSLIGSLRYLTATRP 1148
QK Y DILKKFKM +SK IST + K ++ R + GKRVDST+YKSLIGS+RYL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1149 DIVYGVGLLSRYMEDPCVSHLQGAKRILRYIKGTLTEGIF-----YGNNSDVKLVGYTDS 1203
DIV GVGL SR+ME P H QGA+R LRYIKGTL +GIF N+ D ++V
Sbjct: 559 DIVCGVGLRSRFME-P*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLDTQIV----- 398
Query: 1204 DWAGDTETRKSTSGYAFHLGTGAISWSS 1231
+ GYAFHLG GAISWSS
Sbjct: 397 ----------TRPGYAFHLGRGAISWSS 344
Score = 43.1 bits (100), Expect(2) = 4e-37
Identities = 23/36 (63%), Positives = 25/36 (68%)
Frame = -2
Query: 1234 QHVVALSTAEAEYITATSCATQTVWLRRILEVMHHE 1269
Q VVAL EA YI A SCATQ + L RILEVMH +
Sbjct: 342 QSVVALYANEA*YIAAESCATQAI*LTRILEVMHQK 235
>BP066094
Length = 532
Score = 139 bits (351), Expect(2) = 7e-36
Identities = 71/150 (47%), Positives = 99/150 (65%)
Frame = +2
Query: 929 ARLDTIRMLISLSAQNNWKIHQMDVKSAFLNGTLEEEVYVEQPAGYVVRGKEDKVYRLKK 988
++ + IR+LIS S +N +HQM+VKSAFLNG + EEVYV QP G D +++LKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 989 ALYGLKQAPRAWYKKIDSYFIQNGFQRCPFEHTLYIKFIDPGDVLIVCLYVDDLIFTGNN 1048
+LYGLKQAPRAWY+++ S+ ++N R + TL+ K D+LIV +YVDD+IF N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTY-KDDILIVQIYVDDIIFGSAN 442
Query: 1049 SKMIAEFRGAMISYFEMTDLGLMSYFLGIE 1078
+ EF M + FEM +G + YFLGI+
Sbjct: 443 PSLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 29.6 bits (65), Expect(2) = 7e-36
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = +3
Query: 913 YKQKPGIDYFEVFAPVAR 930
Y Q+ GIDY E FAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV780009
Length = 529
Score = 144 bits (364), Expect = 7e-35
Identities = 71/166 (42%), Positives = 105/166 (62%), Gaps = 2/166 (1%)
Frame = -1
Query: 1170 QGAKRILRYIKGTLTEGIFYGNNSDVKLVGYTDSDWAGDTETRKSTSGYAFHLGTGAISW 1229
Q A R+LRY+KG +G+F+ +S +KL Y+DSDWAG +TR+S +GY+ LGT ISW
Sbjct: 529 QAATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISW 350
Query: 1230 SSKKQHVVALSTAEAEY--ITATSCATQTVWLRRILEVMHHEQNTPTKIYCDNKSAIALS 1287
+KKQ V+ S++EAEY + AT C Q WL + + + P ++CDN+SA+ ++
Sbjct: 349 RTKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYLFQFLKLNVPLPVPLFCDNQSALHIA 176
Query: 1288 KNPVFHGRSKHIDIRFHKIRELIAEKEVVIEYCPTKEQIADIFTKP 1333
NP FH R+KHI++ H +R + + + T Q+ADIFTKP
Sbjct: 175 HNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>AV427921
Length = 284
Score = 113 bits (282), Expect = 2e-25
Identities = 55/93 (59%), Positives = 68/93 (72%)
Frame = +1
Query: 629 QLTTRYTPQQNGVAERKNRTIMDMVRCMLKAKQMPKEFWAEAVATAVYILNRCPTKSVQE 688
Q T YTPQQNGV+ERKNRTI++MVR +L +PK F EAV +++ILNR PT VQ
Sbjct: 4 Q*TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQN 183
Query: 689 KTPEEAGSGRRPSIRHLRVFGCIAYAHVPDQIR 721
PEEA SGR+P++ H R+ C+AYAHVPDQ R
Sbjct: 184 MMPEEAWSGRQPAVDHFRISRCLAYAHVPDQKR 282
>BP084332
Length = 368
Score = 106 bits (265), Expect = 2e-23
Identities = 54/114 (47%), Positives = 78/114 (68%), Gaps = 1/114 (0%)
Frame = +2
Query: 1234 QHVVALSTAEAEYITATSCATQTVWLRRILEVMHH-EQNTPTKIYCDNKSAIALSKNPVF 1292
Q +ALSTAEAEYI+A C+TQ +W++ LE E N P IYCDN +AI+LSKNP+
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIP--IYCDNTAAISLSKNPIL 205
Query: 1293 HGRSKHIDIRFHKIRELIAEKEVVIEYCPTKEQIADIFTKPLKIESFYKLKKML 1346
H R+KHI++++H IR+ + + +++++ T Q ADIFTKPL + F + K L
Sbjct: 206 HSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 89.0 bits (219), Expect = 5e-18
Identities = 48/146 (32%), Positives = 80/146 (53%), Gaps = 8/146 (5%)
Frame = +2
Query: 1005 DSYFIQNGFQRCPFEHTLYIKFIDPGDVLIVCLYVDDLIFTGNNSKMIAEFRGAMISYFE 1064
DS+ + G+ R +H Y K D D +I+ LYVDD++ G N + E + + F+
Sbjct: 5 DSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFD 184
Query: 1065 MTDLGLMSYFLGIEVIQQKDG--IFISQKKYASDILKKFKMEHSKPISTPVEEKLKLTR- 1121
M DLG + LG+++ + + I++SQK Y +L++F M+ PISTP+ KL+
Sbjct: 185 MKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSS 364
Query: 1122 ---ESDGKRVDSTH--YKSLIGSLRY 1142
S+ +R++ + Y S +GSL Y
Sbjct: 365 MIPSSEAERMEMSRVPYASAVGSLMY 442
>BP075943
Length = 547
Score = 86.7 bits (213), Expect = 2e-17
Identities = 50/165 (30%), Positives = 81/165 (48%), Gaps = 1/165 (0%)
Frame = -3
Query: 1030 GDVLIVCLYVDDLIFTGNNSKMIAEFRGAMISYFEMTDLGLMSYFLGIEVIQQKDGIFIS 1089
G + LYVDD++ GN+ I + ++ F + DLG YFLG+E+ + GI ++
Sbjct: 536 GSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLN 357
Query: 1090 QKKYASDILKKFKMEHSKP-ISTPVEEKLKLTRESDGKRVDSTHYKSLIGSLRYLTATRP 1148
Q+KYA ++ +P + L + D Y+ ++G L YL TRP
Sbjct: 356 QRKYALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRP 177
Query: 1149 DIVYGVGLLSRYMEDPCVSHLQGAKRILRYIKGTLTEGIFYGNNS 1193
DI + V LS+++ P H Q L+Y+ G+ G+FY +S
Sbjct: 176 DITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>BP057233
Length = 473
Score = 81.3 bits (199), Expect = 1e-15
Identities = 38/74 (51%), Positives = 51/74 (68%)
Frame = -2
Query: 1276 IYCDNKSAIALSKNPVFHGRSKHIDIRFHKIRELIAEKEVVIEYCPTKEQIADIFTKPLK 1335
++CDN SA AL+ NPV H RSKHI+I H IR+ + + EVV+ Y PT +QIAD TKPL
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1336 IESFYKLKKMLGMM 1349
F +L+ LG++
Sbjct: 265 HTRFSQLRDKLGVI 224
>BP063694
Length = 511
Score = 73.9 bits (180), Expect = 2e-13
Identities = 45/129 (34%), Positives = 66/129 (50%), Gaps = 2/129 (1%)
Frame = -2
Query: 472 SSIIPNDDW-LWHMRFGHFHFSGLNYLSRKEYVSGLPVVKIPSGVCETCQMGKKHRESFP 530
SS +P + LWH R GH +F + L + + ++ P C +CQM K R F
Sbjct: 390 SSPLPRASFELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPI-CCTSCQMAKSKRLVFH 214
Query: 531 TGKSWRAKKLLEIVHSDLCSVE-IPTPGGCRYFITFIDDFSRKAWVYFLKQKSEAVDSFK 589
RA +L+++H DL + + G YF+ F+DDFSR W Y LK+KS+ D
Sbjct: 213 DNNK-RASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLL 37
Query: 590 TFKAFVEKQ 598
FK F+E +
Sbjct: 36 RFKVFMENR 10
>AU088980
Length = 360
Score = 70.5 bits (171), Expect = 2e-12
Identities = 30/105 (28%), Positives = 59/105 (55%)
Frame = +2
Query: 639 NGVAERKNRTIMDMVRCMLKAKQMPKEFWAEAVATAVYILNRCPTKSVQEKTPEEAGSGR 698
N + ERK+R I+++ R ++ +PK W AV A Y++N P+ ++ + P + +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 699 RPSIRHLRVFGCIAYAHVPDQIRKKLDDKGERCIFIGYCSNSKAY 743
P++ L+VFG + Y+ +KLD + +C+F+G+ + + Y
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 70.1 bits (170), Expect = 2e-12
Identities = 34/92 (36%), Positives = 56/92 (59%)
Frame = +1
Query: 1258 WLRRILEVMHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDIRFHKIRELIAEKEVVI 1317
WL +L+ + P +YCDN SA ++ NPVFH R+KHI+I H +RE I + + +
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1318 EYCPTKEQIADIFTKPLKIESFYKLKKMLGMM 1349
+ E +ADI+TK L ++F+++ LG++
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGLI 279
>BP043850
Length = 515
Score = 68.6 bits (166), Expect = 6e-12
Identities = 30/81 (37%), Positives = 50/81 (61%)
Frame = -1
Query: 1254 TQTVWLRRILEVMHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDIRFHKIRELIAEK 1313
++ +WLR +L + Q+ PT ++ DN SAI ++ NPV+H ++HI++ H +RE +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1314 EVVIEYCPTKEQIADIFTKPL 1334
+ + + T QIADI TK L
Sbjct: 335 VITLPHVSTSVQIADILTKSL 273
>AV424544
Length = 276
Score = 67.8 bits (164), Expect = 1e-11
Identities = 34/91 (37%), Positives = 53/91 (57%)
Frame = +3
Query: 1003 KIDSYFIQNGFQRCPFEHTLYIKFIDPGDVLIVCLYVDDLIFTGNNSKMIAEFRGAMISY 1062
K+ SY G+ + +H+L+ KF D ++ +YVDDLI GN+ I + +
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRD-ASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQ 182
Query: 1063 FEMTDLGLMSYFLGIEVIQQKDGIFISQKKY 1093
F + DLG + YFLG+EV + G+F+SQ+KY
Sbjct: 183 FRIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 65.9 bits (159), Expect = 4e-11
Identities = 35/92 (38%), Positives = 54/92 (58%)
Frame = +1
Query: 1258 WLRRILEVMHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDIRFHKIRELIAEKEVVI 1317
WL IL+ + +P+ +YCD++SA ++ N VFH R+KH+DI H +RE + K +
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 1318 EYCPTKEQIADIFTKPLKIESFYKLKKMLGMM 1349
+ +Q ADI TKPL+ F L LG++
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGVL 288
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 43.1 bits (100), Expect(2) = 1e-10
Identities = 17/52 (32%), Positives = 35/52 (66%)
Frame = +3
Query: 1298 HIDIRFHKIRELIAEKEVVIEYCPTKEQIADIFTKPLKIESFYKLKKMLGMM 1349
+I+ ++H +R+ + + ++ +++C T Q+ADI TK LK E F ++ ML ++
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNVV 224
Score = 40.8 bits (94), Expect(2) = 1e-10
Identities = 18/22 (81%), Positives = 20/22 (90%)
Frame = +2
Query: 1279 DNKSAIALSKNPVFHGRSKHID 1300
DNKSAI L+KNPV HGRSKHI+
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV766088
Length = 501
Score = 62.4 bits (150), Expect = 5e-10
Identities = 35/103 (33%), Positives = 54/103 (51%)
Frame = -3
Query: 481 LWHMRFGHFHFSGLNYLSRKEYVSGLPVVKIPSGVCETCQMGKKHRESFPTGKSWRAKKL 540
LWH R GH S L YL +++S + P VCE+C GK R F + +
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKFISYELLNYSP--VCESCVFGKHVRLPFVSSNNVTVMPF 134
Query: 541 LEIVHSDLCSVEIPTPGGCRYFITFIDDFSRKAWVYFLKQKSE 583
+I+HSDL + + + G R+++ F+DDF+ W + L KS+
Sbjct: 133 -DILHSDLWTSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>BP052632
Length = 489
Score = 62.4 bits (150), Expect = 5e-10
Identities = 41/119 (34%), Positives = 62/119 (51%)
Frame = -3
Query: 689 KTPEEAGSGRRPSIRHLRVFGCIAYAHVPDQIRKKLDDKGERCIFIGYCSNSKAYKLYNP 748
KTP E GRRP++ + FGC + + K D+K ++ I +GY SNSKAY ++N
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 749 ETKKVIISRDVTFDEGGMWNWSSKSQKEPIVTPNDYEEEDEHVDTTPDEPDEPETSNRE 807
T+ V S +V FD+ + + + E D ++DE V T +P ETS+ E
Sbjct: 307 RTQVVEESINVKFDD*SI---EATYRLEKDFLNLDLSDQDEEVPTNEGQPS--ETSHEE 146
>TC9278
Length = 743
Score = 58.5 bits (140), Expect = 7e-09
Identities = 31/58 (53%), Positives = 40/58 (68%)
Frame = +1
Query: 835 NFALFADCEPVTFEEASRDENWIKAMDEEINAIEKNKTWELTELPPDKKPIGVKWVYK 892
N FA+CE VTFE A ++W K M EI+ IEKN+T ELT+L D++ I VKWV+K
Sbjct: 541 NLHFFANCELVTFE-ALNGQHWRKVMAGEIHVIEKNET*ELTDLLGDQRLISVKWVHK 711
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 53.1 bits (126), Expect = 3e-07
Identities = 34/126 (26%), Positives = 59/126 (45%)
Frame = +3
Query: 1225 GAISWSSKKQHVVALSTAEAEYITATSCATQTVWLRRILEVMHHEQNTPTKIYCDNKSAI 1284
GA++W S+ Q VALSTAEAE+I AT + +W++ L+ + K+
Sbjct: 21 GAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIVITKALF 200
Query: 1285 ALSKNPVFHGRSKHIDIRFHKIRELIAEKEVVIEYCPTKEQIADIFTKPLKIESFYKLKK 1344
L++ +F + +R+++ K + +E T + AD+ TK L E
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLEVCDM 380
Query: 1345 MLGMMK 1350
+ GM +
Sbjct: 381 IAGMAR 398
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 52.4 bits (124), Expect = 5e-07
Identities = 26/80 (32%), Positives = 46/80 (57%)
Frame = +3
Query: 1255 QTVWLRRILEVMHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDIRFHKIRELIAEKE 1314
+ +WL L + IYCDN+SA+ L+ N VFH R+++I+I H + +
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1315 VVIEYCPTKEQIADIFTKPL 1334
+ + + P+ +Q+AD+FTK +
Sbjct: 201 LHLLHVPSSDQVADVFTKTI 260
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,495,410
Number of Sequences: 28460
Number of extensions: 340295
Number of successful extensions: 1957
Number of sequences better than 10.0: 115
Number of HSP's better than 10.0 without gapping: 1719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1853
length of query: 1351
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1250
effective length of database: 2,023,140
effective search space: 2528925000
effective search space used: 2528925000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148918.3