BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.4 - phase: 0
(1009 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV778022 204 4e-53
TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , par... 88 6e-18
AV406876 33 0.17
TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prot... 33 0.23
BP048490 32 0.50
TC16796 similar to UP|Q7XYX7 (Q7XYX7) AKIN beta2, partial (55%) 31 0.86
TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,... 31 0.86
TC16774 31 0.86
TC8156 UP|AAR99188 (AAR99188) ATP synthase F0 subunit 8, partial... 31 0.86
TC15185 30 1.5
BP061810 30 1.5
TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine ... 30 2.5
TC10820 weakly similar to UP|Q9M9Y0 (Q9M9Y0) F4H5.19 protein, pa... 29 3.3
AV776363 29 3.3
TC18008 weakly similar to UP|O49609 (O49609) Beta-galactosidase ... 29 4.3
BP050564 29 4.3
TC8599 homologue to UP|Q40478 (Q40478) EREBP-4, partial (26%) 29 4.3
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 29 4.3
AV778797 29 4.3
BP055885 28 5.6
>AV778022
Length = 554
Score = 204 bits (520), Expect = 4e-53
Identities = 110/162 (67%), Positives = 128/162 (78%)
Frame = +3
Query: 1 MRSGAYTLQETLTAEAASILKHSLGLARRRGHAQITPLHVAATLLSLPSSSLRNACLKSQ 60
MR+G T+Q+TLT EAA++L HS+ A RR H Q TPLHVAATLL+ PS LR AC+KS
Sbjct: 90 MRAGLSTIQQTLTPEAANVLNHSIAEAGRRNHGQTTPLHVAATLLASPSGYLRQACIKSH 269
Query: 61 QQNNHPLQCRALELCFNVALNRLPTTTTSPLLQPQHVPSLSNALIAALKRAQAHQRRGCI 120
++HPLQCRALELCF+VAL RLPT+ +S P P +SNAL+AALKRAQAHQRRG
Sbjct: 270 PNSSHPLQCRALELCFSVALERLPTSQSS---SPGMEPPISNALMAALKRAQAHQRRGYP 440
Query: 121 EQNQQQQQQPLLSVKVELDQLILSILDDPSVSRVMREAGFSS 162
E QQQQPLL+VKVEL+QLI+SILDDPSVSRVMREA FSS
Sbjct: 441 E----QQQQPLLAVKVELEQLIISILDDPSVSRVMREASFSS 554
>TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , partial (28%)
Length = 1001
Score = 88.2 bits (217), Expect = 6e-18
Identities = 74/247 (29%), Positives = 119/247 (47%), Gaps = 1/247 (0%)
Frame = +3
Query: 26 LARRRGHAQITPLHVAATLLSLPSSSLRNACLKSQQQNNHPLQCRALELCFNVALNRLPT 85
LA GHAQ+TPLH+A+TL+S P+ A S + + RA+E N AL +LP+
Sbjct: 258 LAMSSGHAQMTPLHLASTLISDPNGIFFQAISNSSGEES----ARAVERVLNQALKKLPS 425
Query: 86 TTTSPLLQPQHVPSLSNALIAALKRAQAHQR-RGCIEQNQQQQQQPLLSVKVELDQLILS 144
+ P +P+ S LI A++RAQA Q+ RG + +DQLIL
Sbjct: 426 QSP----PPDEIPA-STTLIKAIRRAQAAQKSRG--------------DTHLAVDQLILG 548
Query: 145 ILDDPSVSRVMREAGFSSPSVKNNLENSSTLINSSSVFHSSPSPLSHNHFLSSYGYGSVL 204
IL+D + +++EAG ++ VK+ L+ + S S + L +YG ++
Sbjct: 549 ILEDSQIGDLLKEAGVAAAKVKSELDKLRGKVGKKV---ESASGDTTFQALKTYG-RDLV 716
Query: 205 FSSQKKEQVVYHPFLKSSESNKEDINLVFDVLLRKKKKNTVIVGDTVSLTEGLVSEIMKR 264
+ K + V+ E+I V +L R+ K N V++G+ +V + +R
Sbjct: 717 EQAGKLDPVI---------GRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQR 869
Query: 265 FERGEVP 271
RG+VP
Sbjct: 870 IVRGDVP 890
>AV406876
Length = 234
Score = 33.5 bits (75), Expect = 0.17
Identities = 27/73 (36%), Positives = 38/73 (51%), Gaps = 3/73 (4%)
Frame = +3
Query: 316 LGVGAIFYVGDLKWIVD-DNDGSLNEKE-VVDYVVEEIGKLFGEEGNKNGKIWLV-ATAS 372
+G G GDLK V D+DG E V +VV E+ KL ++ WL+ A AS
Sbjct: 21 VGPGVAVNFGDLKGFVGGDSDGCGGSGEDAVRFVVGELAKLLKVHCDR---FWLLGAAAS 191
Query: 373 YQSYMRCQMRVPT 385
Y+SY++ R P+
Sbjct: 192 YESYLKFLGRFPS 230
>TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (33%)
Length = 908
Score = 33.1 bits (74), Expect = 0.23
Identities = 44/172 (25%), Positives = 74/172 (42%), Gaps = 12/172 (6%)
Frame = +1
Query: 624 TLQRDHIYKV---LQENIPWHCETVSSIAEALVDSK-----SSKECATWLFLQGNDSVGK 675
T + D + K+ L + I E V +I+ A+ ++ ++ A+++F G VGK
Sbjct: 406 TDESDRLLKMEDTLHKRIIGQDEAVKAISRAIRRARVGLKNPNRPIASFIF-SGPTGVGK 582
Query: 676 KRLALAIAESVFGSVEMFSHVDMMKRENSETPFSEKVVGPLKNNEKFVVLVENADFGDTL 735
LA A+A FGS E +DM E E K++G + +V E + +
Sbjct: 583 SELAKALAAYYFGSEEAMIRLDM--SEFMERHTVSKLIG---SPPGYVGYTEGGQLTEAV 747
Query: 736 IRK----MLADEFEIAKFGTLGQKIFILSNGGSMVSEDQKKDSVMKLVLKIS 783
R+ +L DE E A + IL +G S+ + D L++ S
Sbjct: 748 RRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTS 903
>BP048490
Length = 452
Score = 32.0 bits (71), Expect = 0.50
Identities = 26/112 (23%), Positives = 46/112 (40%)
Frame = +3
Query: 833 KREFSFSRQSSFNNTLDLNMKADEEDNEDYDEGENSPISSDLTRETLGEHLISNESLDSI 892
K+ S ++ D +K D+ D ++ D E+S + + +T + + E +D+
Sbjct: 108 KKSASSDSDDEDSSDSDSEVKKDKMDVDESDSSEDSDEEDEKSTKTPQKKVKDVEMVDA- 284
Query: 893 ENLFEFNQSPAKNKEMTQMFMSRVKESFEEVLGNVKFSVQDKVIEEIGVGCG 944
S KN T + + S +GN+ FSVQ +E CG
Sbjct: 285 --------SSGKNAPKTPAAQTENEGSKTLFVGNLSFSVQRSDVENFFKDCG 416
>TC16796 similar to UP|Q7XYX7 (Q7XYX7) AKIN beta2, partial (55%)
Length = 746
Score = 31.2 bits (69), Expect = 0.86
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 299 EMNVIRVLKRKVSDYVALGVGAIFYVGDLKWIVDDNDGSLNEKEVVDYVVEEIGKLFGEE 358
+ +++VL V Y + G Y +L W DD + N ++ +YV E+IG + E
Sbjct: 23 DFTIMKVLPSGVYQYRFVVDGQWRYAPELPWAQDDAGNAYNILDLQEYVPEDIGGISSFE 202
Query: 359 GNKN 362
K+
Sbjct: 203 PPKS 214
>TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1, partial
(27%)
Length = 1289
Score = 31.2 bits (69), Expect = 0.86
Identities = 27/83 (32%), Positives = 36/83 (42%), Gaps = 2/83 (2%)
Frame = +3
Query: 470 QSHSTEARQKDELTQLNKKWNRLCQCLHQNKQPQNHWSNNHSSNAKIYPYNSSYPYWPNQ 529
QSHS DE+T + R Q +++ Q WS + SS +K P P W
Sbjct: 363 QSHSHLPNTSDEIT-----YRRGPQPDLRSRACQPPWSTSSSSRSKTTPTPPRSPPWSTA 527
Query: 530 GSSILPDTSSSIS--FADSATKP 550
+ P T+SS S SAT P
Sbjct: 528 SAPSSP*TTSSTSRWAQSSATAP 596
>TC16774
Length = 529
Score = 31.2 bits (69), Expect = 0.86
Identities = 25/75 (33%), Positives = 37/75 (49%)
Frame = +1
Query: 174 TLINSSSVFHSSPSPLSHNHFLSSYGYGSVLFSSQKKEQVVYHPFLKSSESNKEDINLVF 233
+ ++ SS+F + S LS NH L G + F S+ +YHP KS N + I
Sbjct: 94 SFLSLSSIFLAPRSTLSSNHGLIFSGKFRLQF-SRPSSFPIYHPSSKSL-PNSDFIVSTI 267
Query: 234 DVLLRKKKKNTVIVG 248
D ++RK + V VG
Sbjct: 268 DSVIRKLQFKCVCVG 312
>TC8156 UP|AAR99188 (AAR99188) ATP synthase F0 subunit 8, partial (24%)
Length = 635
Score = 31.2 bits (69), Expect = 0.86
Identities = 19/58 (32%), Positives = 33/58 (56%), Gaps = 5/58 (8%)
Frame = +2
Query: 76 FNVALNRLPTTTTSPLLQP-QHVPSLSNALIAALKRAQA----HQRRGCIEQNQQQQQ 128
F++A N TTTSP +P +HVP + A ++ R+QA + G + + +Q++Q
Sbjct: 389 FSMATNENAATTTSPSRKPHRHVPPSTQATTSSSSRSQATTTNREHHGELHE*EQRKQ 562
>TC15185
Length = 876
Score = 30.4 bits (67), Expect = 1.5
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Frame = +3
Query: 934 KVIEEIGVGCGSFTNN---MFEKWLKGIFQTSLERV--NGGDKNGIVYTL 978
++ E++ G+F N M E+W G+F+++L RV NG ++ I Y L
Sbjct: 114 QIWEDVAPLKGAFIVNLGDMLERWSNGVFKSTLHRVLGNGQERYSIAYFL 263
>BP061810
Length = 498
Score = 30.4 bits (67), Expect = 1.5
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Frame = -1
Query: 934 KVIEEIGVGCGSFTNN---MFEKWLKGIFQTSLERV--NGGDKNGIVYTL 978
++ E++ G+F N M E+W G+F+++L RV NG ++ I Y L
Sbjct: 474 QIWEDVAPLKGAFIVNLGDMLERWSNGVFKSTLHRVLGNGQERYSIAYFL 325
>TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine synthetase
1 (Methionine adenosyltransferase 1) (AdoMet synthetase
1) , partial (67%)
Length = 862
Score = 29.6 bits (65), Expect = 2.5
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = -3
Query: 467 SWLQSHSTEARQKDELTQLNKKWNRLCQCLHQNKQPQNHWSNNHS 511
+W Q H +EA + ++ ++ C HQ++ PQ WSN H+
Sbjct: 506 AW*QGHGSEASAQGS----HR*HSQTCDPGHQHQFPQASWSNGHA 384
>TC10820 weakly similar to UP|Q9M9Y0 (Q9M9Y0) F4H5.19 protein, partial (7%)
Length = 619
Score = 29.3 bits (64), Expect = 3.3
Identities = 17/45 (37%), Positives = 25/45 (54%)
Frame = -1
Query: 163 PSVKNNLENSSTLINSSSVFHSSPSPLSHNHFLSSYGYGSVLFSS 207
P++KNN+ ++ T SS+ + S L HFL+ SVL SS
Sbjct: 358 PAIKNNVHDAQTA*RSSNEINFGYSSL*SPHFLTRLVLCSVLLSS 224
>AV776363
Length = 427
Score = 29.3 bits (64), Expect = 3.3
Identities = 19/57 (33%), Positives = 30/57 (52%)
Frame = -3
Query: 933 DKVIEEIGVGCGSFTNNMFEKWLKGIFQTSLERVNGGDKNGIVYTLCWGGKEDRKWD 989
+++ E V G T N+ +FQTS ++GG + G + LCWGG+ R+ D
Sbjct: 302 ERLPEPGAVAIGPRTRNL-------VFQTSFLYLDGG*EPG--WCLCWGGRC*RRLD 159
>TC18008 weakly similar to UP|O49609 (O49609) Beta-galactosidase - like
protein (Beta-galactosidase-like protein), partial (8%)
Length = 301
Score = 28.9 bits (63), Expect = 4.3
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 507 SNNHSSNAKIYPYNSSYPYWPNQGSSILPDTSSSI 541
+NNH+ AK + S Y P + SILPD + +
Sbjct: 136 TNNHTKTAKSINFKGSKYYLPPRSISILPDCKTVV 240
>BP050564
Length = 490
Score = 28.9 bits (63), Expect = 4.3
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 56 CLKSQQQNNHPLQCRALELCFNVALNRLPTTTTSPLL 92
CLK + HP+ + F++ P TT SPL+
Sbjct: 27 CLKEMCSHLHPISSFSFSFSFSITNRTSPPTTASPLI 137
>TC8599 homologue to UP|Q40478 (Q40478) EREBP-4, partial (26%)
Length = 632
Score = 28.9 bits (63), Expect = 4.3
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 3/77 (3%)
Frame = +3
Query: 789 PTFELSPSSSSSSKSPCLGNKRSAELDLFSKIKIPRIE---ENEGNKKREFSFSRQSSFN 845
P+F P SSS+ L N++ + I IP I ++ ++ +E SFS QSS +
Sbjct: 192 PSFSKPPRSSSN-----LSNRKPS-----LNISIPSINSGLKSNISQSKETSFSHQSSSS 341
Query: 846 NTLDLNMKADEEDNEDY 862
++ + +E++N+ Y
Sbjct: 342 SSSSDHQTQEEDNNKHY 392
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 28.9 bits (63), Expect = 4.3
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = +1
Query: 114 HQRRGCIEQNQQQQQQPLLSVKVELDQLILSILDDPSVSRVMREAGFSSPSVKNN 168
HQ + I+Q QQQQQQP+ + ++ Q S MR A SSPS K+N
Sbjct: 136 HQFQQPIQQQQQQQQQPIQQPQQQMQQQ-QQEQHQQSGDLKMRSA-TSSPSPKDN 294
>AV778797
Length = 558
Score = 28.9 bits (63), Expect = 4.3
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 56 CLKSQQQNNHPLQCRALELCFNVALNRLPTTTTSPLL 92
CLK + HP+ + F++ P TT SPL+
Sbjct: 51 CLKEMCSHLHPISSFSFSFSFSITNRTSPPTTASPLI 161
>BP055885
Length = 464
Score = 28.5 bits (62), Expect = 5.6
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = +3
Query: 494 QCLHQNKQPQNHWSNNHSSNAKIYP 518
+C H + P + W+ NH KI+P
Sbjct: 135 ECYHPH*LPSSQWAQNHQRGPKIHP 209
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.129 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,923,128
Number of Sequences: 28460
Number of extensions: 256455
Number of successful extensions: 1596
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 1552
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1587
length of query: 1009
length of database: 4,897,600
effective HSP length: 99
effective length of query: 910
effective length of database: 2,080,060
effective search space: 1892854600
effective search space used: 1892854600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148819.4