BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.1 + phase: 0
(244 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9040 similar to UP|RL71_ARATH (P60040) 60S ribosomal protein L... 441 e-125
TC11805 weakly similar to SP|Q42208|RL7_ARATH 60S ribosomal prot... 211 9e-56
TC13051 weakly similar to PIR|S04849|R5DO7 ribosomal protein L7 ... 132 7e-32
TC11712 similar to SP|Q42208|RL7_ARATH 60S ribosomal protein L7.... 69 2e-20
BP085298 72 9e-14
BP065779 69 6e-13
AV767352 64 3e-11
TC13389 similar to SP|Q42208|RL7_ARATH 60S ribosomal protein L7.... 39 7e-07
BP075055 35 0.012
TC12630 similar to PIR|T39903|T39903 serine-rich protein - fissi... 30 0.49
TC17076 29 0.84
TC8505 similar to UP|Q8LG02 (Q8LG02) Nitrate transporter, partia... 27 4.2
AV423294 26 7.1
TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), p... 25 9.3
TC14869 25 9.3
TC11158 similar to UP|Q39682 (Q39682) Glycine-rich protein (Frag... 25 9.3
>TC9040 similar to UP|RL71_ARATH (P60040) 60S ribosomal protein L7-1,
complete
Length = 941
Score = 441 bits (1133), Expect = e-125
Identities = 215/244 (88%), Positives = 232/244 (94%)
Frame = +1
Query: 1 MAKEEVKTVVPESVLKKQKRNEEWALVKKQEQESAKKKRSETRKLIWSRAKQYAKEYDDS 60
++KEEVK VVPESVLKKQKRNEEWAL KQ+ E+AKKKR+ +RKLIWSRA+QYAKEYD+
Sbjct: 52 VSKEEVKAVVPESVLKKQKRNEEWALATKQQLEAAKKKRAVSRKLIWSRAQQYAKEYDEQ 231
Query: 61 QKELIALKREAKLKGGFYVDPEAKLLFIIRIRGINAMDPKSRKILQLLRLRQICNGVFLK 120
QKEL+ LKREAKLKGGFYV+PEAKLLFI+RIRGINAM PK+RKILQLLRLRQI NGVFLK
Sbjct: 232 QKELVRLKREAKLKGGFYVNPEAKLLFIVRIRGINAMHPKTRKILQLLRLRQIFNGVFLK 411
Query: 121 VNKATVNMLHRVEPYVTYGYPNLKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGI 180
VNKATVNML RVEPYVTYGYPNLKS+RELIYKRGYGKV++QRIALTDNS+IEQ LGKHGI
Sbjct: 412 VNKATVNMLQRVEPYVTYGYPNLKSIRELIYKRGYGKVNKQRIALTDNSVIEQTLGKHGI 591
Query: 181 ICIEDLIHEILTVGPHFREANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELI 240
ICIEDLIHEILTVGPHFREANNFLWPFKLKAPLGG+KKKRNHYVEGGDAG REDYINELI
Sbjct: 592 ICIEDLIHEILTVGPHFREANNFLWPFKLKAPLGGLKKKRNHYVEGGDAGXREDYINELI 771
Query: 241 RRMN 244
RRMN
Sbjct: 772 RRMN 783
>TC11805 weakly similar to SP|Q42208|RL7_ARATH 60S ribosomal protein L7.
{Arabidopsis thaliana;}, partial (43%)
Length = 829
Score = 211 bits (537), Expect = 9e-56
Identities = 106/242 (43%), Positives = 155/242 (63%)
Frame = +2
Query: 3 KEEVKTVVPESVLKKQKRNEEWALVKKQEQESAKKKRSETRKLIWSRAKQYAKEYDDSQK 62
K ++ T+ ++ K NE AL KK + + + I + + + EY + +
Sbjct: 80 KPKLSTLFQRLHSRRGKNNEALALRKKAQFQYKNFQIRNNNDSI-KKPEDFIFEYRNQEL 256
Query: 63 ELIALKREAKLKGGFYVDPEAKLLFIIRIRGINAMDPKSRKILQLLRLRQICNGVFLKVN 122
+LI +KR K + P +K + +IRI+G M P +RK L L LR+I + VF+K +
Sbjct: 257 DLIRMKRRVKRNPSELLTPNSKPIIVIRIQGKKDMHPVTRKALFSLGLRKIFSAVFVKPS 436
Query: 123 KATVNMLHRVEPYVTYGYPNLKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIIC 182
+ + L RVEP+VTYGYPNLKS++EL+YK+G+ K+D++++ LTDN+IIEQ LGK GI+C
Sbjct: 437 EGIMAKLQRVEPFVTYGYPNLKSIKELLYKKGHAKIDKRKVPLTDNNIIEQELGKFGIVC 616
Query: 183 IEDLIHEILTVGPHFREANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRR 242
+ED++HEI GPHF+E F+WPF L P G+K + Y +GGDAGNRED INELI +
Sbjct: 617 MEDMVHEIENTGPHFKEVVRFMWPFDLNKPSDGLKGSKTAYKDGGDAGNREDSINELINK 796
Query: 243 MN 244
MN
Sbjct: 797 MN 802
>TC13051 weakly similar to PIR|S04849|R5DO7 ribosomal protein L7 - slime
mold (Dictyostelium discoideum) {Dictyostelium
discoideum;}, partial (35%)
Length = 517
Score = 132 bits (331), Expect = 7e-32
Identities = 58/104 (55%), Positives = 80/104 (76%)
Frame = +3
Query: 140 YPNLKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEILTVGPHFRE 199
YPNL+S++EL+YK+G+ K+D ++++LTD SII Q LGK GI+C+ED++H+I VGPHF+E
Sbjct: 63 YPNLESIKELLYKKGHAKIDERKVSLTD-SIIGQELGKFGILCMEDMVHQIYNVGPHFKE 239
Query: 200 ANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRRM 243
F+WPF+L P G K + + GGDAGNRED INELI +M
Sbjct: 240 VVRFIWPFELNKPSDGFKGSKTLFKNGGDAGNREDSINELINKM 371
>TC11712 similar to SP|Q42208|RL7_ARATH 60S ribosomal protein L7.
{Arabidopsis thaliana;}, partial (21%)
Length = 408
Score = 68.9 bits (167), Expect(2) = 2e-20
Identities = 41/74 (55%), Positives = 49/74 (65%)
Frame = +2
Query: 20 RNEEWALVKKQEQESAKKKRSETRKLIWSRAKQYAKEYDDSQKELIALKREAKLKGGFYV 79
RN W +KK E E+AKKKR+E+ KL R KE D+ + LI LK + KLK FYV
Sbjct: 68 RNVPW--LKK*ELEAAKKKRAESCKLAPPRLSSMPKECDEQTQ*LILLKCKGKLKS*FYV 241
Query: 80 DPEAKLLFIIRIRG 93
DP+AKLLFIIRI G
Sbjct: 242 DPDAKLLFIIRIPG 283
Score = 45.8 bits (107), Expect(2) = 2e-20
Identities = 22/42 (52%), Positives = 31/42 (73%)
Frame = +3
Query: 93 GINAMDPKSRKILQLLRLRQICNGVFLKVNKATVNMLHRVEP 134
G+N + PK+R+IL LLRLRQI NGVFLKV++ + ++ P
Sbjct: 282 GLNPLHPKTRRILLLLRLRQIFNGVFLKVSQLHHEPVPKLSP 407
Score = 28.1 bits (61), Expect = 1.4
Identities = 20/52 (38%), Positives = 28/52 (53%)
Frame = +1
Query: 4 EEVKTVVPESVLKKQKRNEEWALVKKQEQESAKKKRSETRKLIWSRAKQYAK 55
EEVK +PES K K EE AL +K S +++S ++ KQYA+
Sbjct: 13 EEVKASIPESAPAKHKWVEECALAEKVGA*SC*EEKS*EL*AGPAQVKQYAQ 168
>BP085298
Length = 393
Score = 72.0 bits (175), Expect = 9e-14
Identities = 46/106 (43%), Positives = 64/106 (59%), Gaps = 2/106 (1%)
Frame = -3
Query: 22 EEWALVKKQEQESAKKKRSETRKLIWSRAK--QYAKEYDDSQKELIALKREAKLKGGFYV 79
EE + ++ + +R E+R + S + + + + + ELI LK E KLKG FYV
Sbjct: 334 EEECRISEKVRSLKLLRRKESRVVP*STVELTRMPRSRHEPENELI*LKCEEKLKGRFYV 155
Query: 80 DPEAKLLFIIRIRGINAMDPKSRKILQLLRLRQICNGVFLKVNKAT 125
D AKLLF IR+ GIN M PK+R+IL+L RLR GVFL V ++T
Sbjct: 154 DSNAKLLFNIRLCGINPMHPKTRRILKLSRLRPFFYGVFL*VYQST 17
Score = 27.7 bits (60), Expect = 1.9
Identities = 16/26 (61%), Positives = 19/26 (72%)
Frame = -1
Query: 4 EEVKTVVPESVLKKQKRNEEWALVKK 29
EEV+T VP S +K+KRN ALVKK
Sbjct: 378 EEVRTFVPPSFPEKRKRNV--ALVKK 307
>BP065779
Length = 424
Score = 69.3 bits (168), Expect = 6e-13
Identities = 38/80 (47%), Positives = 50/80 (62%), Gaps = 1/80 (1%)
Frame = -1
Query: 161 QRIALTDNSIIEQVLGKHGIICIEDLIHEILTVGPH-FREANNFLWPFKLKAPLGGMKKK 219
+R AL DNS+I + LGK+GI+ PH + FLWPFK K PLG +KK+
Sbjct: 382 KRTALIDNSVIGEALGKYGIM-------RPYPWDPHCWASXTTFLWPFKXKVPLG*LKKE 224
Query: 220 RNHYVEGGDAGNREDYINEL 239
R++YVE GDA R +Y+NEL
Sbjct: 223 RSYYVEEGDAIIRGNYVNEL 164
>AV767352
Length = 236
Score = 63.5 bits (153), Expect = 3e-11
Identities = 30/38 (78%), Positives = 33/38 (85%)
Frame = -1
Query: 207 FKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRRMN 244
FKLKAPLGG+ KKRN Y GGDAG+R+D INELIRRMN
Sbjct: 236 FKLKAPLGGLNKKRNLYF*GGDAGHRKDVINELIRRMN 123
>TC13389 similar to SP|Q42208|RL7_ARATH 60S ribosomal protein L7.
{Arabidopsis thaliana;}, partial (7%)
Length = 544
Score = 39.3 bits (90), Expect(2) = 7e-07
Identities = 18/34 (52%), Positives = 20/34 (57%)
Frame = +3
Query: 193 VGPHFREANNFLWPFKLKAPLGGMKKKRNHYVEG 226
+G R NFLWPFK KAPL K+ YVEG
Sbjct: 267 LGLILRRQTNFLWPFKFKAPLSRXKEXGTTYVEG 368
Score = 33.5 bits (75), Expect = 0.034
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +2
Query: 185 DLIHEILTVGPHFREANNFLWPFKLKAPLGGMKKKRNH 222
D IHEI+TV PHF+EAN ++ + RNH
Sbjct: 242 DRIHEIMTVRPHFKEANKLPLAI*VQGSFVAX*RXRNH 355
Score = 29.3 bits (64), Expect(2) = 7e-07
Identities = 11/20 (55%), Positives = 17/20 (85%)
Frame = +1
Query: 162 RIALTDNSIIEQVLGKHGII 181
R +L DNS+IE+ +GK+GI+
Sbjct: 181 RTSLIDNSVIEEAIGKYGIM 240
>BP075055
Length = 470
Score = 35.0 bits (79), Expect = 0.012
Identities = 26/74 (35%), Positives = 38/74 (51%)
Frame = -2
Query: 15 LKKQKRNEEWALVKKQEQESAKKKRSETRKLIWSRAKQYAKEYDDSQKELIALKREAKLK 74
LKK KR E ALV+ + E ++K + + KE ++ ++E+I +LK
Sbjct: 241 LKK*KRGEGCALVQSKSLELLRRK**RIVR*STIGLSRALKECNEKEREMI------QLK 80
Query: 75 GGFYVDPEAKLLFI 88
G FYVDP KL I
Sbjct: 79 G*FYVDPNDKLSLI 38
>TC12630 similar to PIR|T39903|T39903 serine-rich protein - fission yeast
(Schizosaccharomyces pombe)
{Schizosaccharomyces pombe;}, partial (6%)
Length = 652
Score = 29.6 bits (65), Expect = 0.49
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 3/46 (6%)
Frame = -2
Query: 19 KRNEEWALVKKQ---EQESAKKKRSETRKLIWSRAKQYAKEYDDSQ 61
KRNEE A +KKQ E S K++ + L+ S KQ +D+ +
Sbjct: 594 KRNEEIAAIKKQHSDEMTSVKREMEGLKFLVKSLLKQQNPNFDEGE 457
>TC17076
Length = 644
Score = 28.9 bits (63), Expect = 0.84
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 10/50 (20%)
Frame = +3
Query: 139 GYPNLKSVRELIYKRGYG----------KVDRQRIALTDNSIIEQVLGKH 178
G NL++ L+ ++GYG + + +R LTD +IEQ KH
Sbjct: 177 GLHNLRNAEMLVMEKGYGGRYSLLMHHDEQELRRFGLTDEMLIEQEWHKH 326
>TC8505 similar to UP|Q8LG02 (Q8LG02) Nitrate transporter, partial (23%)
Length = 1495
Score = 26.6 bits (57), Expect = 4.2
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = -3
Query: 86 LFIIRIRGINAMDPKSRKILQLLRLRQICNGVFLKVNKATV-NMLHRVEPYV 136
L+I +RGI R+ L+ LRL + CN ++ N TV ML +V+ V
Sbjct: 1331 LYITLVRGIPGTRRLHRRTLEALRLGK-CNRTVMRWNTPTVRGMLQQVKRLV 1179
>AV423294
Length = 459
Score = 25.8 bits (55), Expect = 7.1
Identities = 13/40 (32%), Positives = 22/40 (54%)
Frame = +3
Query: 154 GYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEILTV 193
G+GKV R I + + + L HG+ I + + E+LT+
Sbjct: 9 GFGKVYRGFIERINQVVAIKQLNPHGLQGIREFVVEVLTL 128
>TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), partial
(63%)
Length = 1130
Score = 25.4 bits (54), Expect = 9.3
Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +3
Query: 12 ESVLKKQKRNEEWALVKKQEQESAKKKRSETRKLIWSR--AKQYAKE 56
E+V KK+K+N + K+ K +S TRK+ ++ AK+ KE
Sbjct: 654 ENVQKKKKKNTKGEKQNKKRLGKKKSDKSNTRKMRSTKRQAKKSRKE 794
>TC14869
Length = 828
Score = 25.4 bits (54), Expect = 9.3
Identities = 15/51 (29%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = -3
Query: 23 EWALVKKQEQESAKKKRSETRKLIWSRAKQYAKEYDDSQKELIALK-REAK 72
EW+ V + +E +++ ETR+ I KQ + ++ ++ ++A K RE K
Sbjct: 265 EWSFVIGRRREIGEEEEEETRERI---EKQSRRRREEEEEAMVAEKGREGK 122
>TC11158 similar to UP|Q39682 (Q39682) Glycine-rich protein (Fragment),
partial (22%)
Length = 357
Score = 25.4 bits (54), Expect = 9.3
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = -2
Query: 21 NEEWALVKKQEQESAKKKRSET 42
+EEW V+ + +E KKK+ ET
Sbjct: 290 DEEWGNVEMRRKEKKKKKKVET 225
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.138 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,546,190
Number of Sequences: 28460
Number of extensions: 41847
Number of successful extensions: 242
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of query: 244
length of database: 4,897,600
effective HSP length: 88
effective length of query: 156
effective length of database: 2,393,120
effective search space: 373326720
effective search space used: 373326720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC148819.1