BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148775.4 - phase: 0
(287 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 162 1e-42
TC17355 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (34%) 128 1e-30
TC8690 similar to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase precu... 107 2e-24
AV426815 100 2e-22
CN825195 83 6e-17
TC9501 weakly similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesteras... 80 4e-16
BP039243 73 5e-14
TC19984 similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase , pa... 69 1e-12
BP033848 63 5e-11
TC10949 similar to UP|PME3_PHAVU (Q43111) Pectinesterase 3 precu... 52 1e-07
AU089161 35 3e-05
AU088824 42 9e-05
AV426324 40 4e-04
AU088938 36 0.009
AV776418 28 1.4
TC8721 28 1.8
AV771126 27 4.0
AV776199 27 5.2
TC18302 similar to UP|Q8L6T7 (Q8L6T7) Ring-H2 zinc finger protei... 26 6.8
TC17888 similar to UP|Q9FJA0 (Q9FJA0) Gb|AAD21756.1, partial (9%) 26 8.9
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 162 bits (409), Expect(2) = 1e-42
Identities = 94/192 (48%), Positives = 125/192 (64%), Gaps = 5/192 (2%)
Frame = +2
Query: 57 DGSGNYMKIMDAVMAAPNGSKKRYVIHIKKGVYNEHVMINNSKSNLMMIGDGMGATVITG 116
DGSGNY +M+AV+AAP+ S KR+VI++KKGVY E+ I K N+MM+GDGM TVI+G
Sbjct: 29 DGSGNYTTVMEAVLAAPDYSMKRFVIYVKKGVYEEYAEIKKKKWNIMMVGDGMDVTVISG 208
Query: 117 DLSWGRDKLDTSYTYTFGVEGLGFSAQDISFRNTAWPENHQAVALLSDSDTSVFYRCEIS 176
+ S+G D T + TF V G GF A+ I+F+NTA PE HQAVAL SDSD +VF+RC I+
Sbjct: 209 NRSFG-DGWTTFKSATFAVSGRGFIARGITFQNTAGPEKHQAVALRSDSDLAVFFRCSIT 385
Query: 177 GFQDSLCANIKHHSIRIAKSEARLT---SYLVRQLSSF--KTDILVRKGPTGQQNTITAQ 231
G+QDSL H++R E R+T ++ ++ +I RKG Q+NTITAQ
Sbjct: 386 GYQDSLYP----HTMRQFYKECRITGTVDFIFGDATAVFQNCNIQARKGLQDQKNTITAQ 553
Query: 232 GGPEKPNLPFGF 243
G + P GF
Sbjct: 554 GRKD-PGQSSGF 586
Score = 27.3 bits (59), Expect(2) = 1e-42
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +3
Query: 244 AFQFCNVCADPEFLPFV 260
+FQ+CN+ AD E LP V
Sbjct: 588 SFQYCNITADSELLPSV 638
>TC17355 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (34%)
Length = 848
Score = 128 bits (321), Expect = 1e-30
Identities = 78/217 (35%), Positives = 121/217 (54%), Gaps = 6/217 (2%)
Frame = +1
Query: 51 DAVVAS--DGSGNYMKIMDAVMAAPNGSKKR---YVIHIKKGVYNEHVMINNSKSNLMMI 105
D V+ S +G+ NY I DA+ AAPN +K + ++I++++G Y E+V++ K N++++
Sbjct: 148 DFVIVSPYNGTDNYTSIGDAIAAAPNHTKPQDGYFLIYVREGYYEEYVIVPKEKKNILLV 327
Query: 106 GDGMGATVITGDLSWGRDKLDTSYTYTFGVEGLGFSAQDISFRNTAWPENHQAVALLSDS 165
GDG+ T+ITG+ S D T + TF V G F+A D++FRNTA P HQAVA+ +++
Sbjct: 328 GDGINKTIITGNHSV-IDGWTTFNSSTFAVSGERFTAVDVTFRNTAGPAKHQAVAVRNNA 504
Query: 166 DTSVFYRCEISGFQDSLCANIKHHSIRIAKSEARLTSYLVRQLSSFKT-DILVRKGPTGQ 224
D S FYRC G+QD+L + R + F+ +I RK Q
Sbjct: 505 DLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNIYARKPLPFQ 684
Query: 225 QNTITAQGGPEKPNLPFGFAFQFCNVCADPEFLPFVN 261
+N +TAQG + PN G + Q C + A P+ +N
Sbjct: 685 KNAVTAQGRTD-PNQNTGISIQNCTIDAAPDLAAELN 792
>TC8690 similar to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase precursor
(Pectinesterase) , partial (59%)
Length = 1082
Score = 107 bits (268), Expect = 2e-24
Identities = 59/122 (48%), Positives = 80/122 (65%), Gaps = 5/122 (4%)
Frame = +3
Query: 29 GKFPSWVKPGDRKLLQASA--VPADAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKK 86
G FPSWV DR+LL++S V A+ VVA DGSG + + +AV +AP+ K RYVI++KK
Sbjct: 729 GDFPSWVTRKDRRLLESSVGDVNANVVVAKDGSGRFKTVAEAVASAPDSGKTRYVIYVKK 908
Query: 87 GVYNEHVMINNSKSNLMMIGDGMGATVITGDLSWGRDKLDTSYTY---TFGVEGLGFSAQ 143
G Y E++ I K+N+M+ GDGM AT+ITG+L + +D S T+ T G GF AQ
Sbjct: 909 GTYKENIEIGKKKTNVMLTGDGMDATIITGNL----NVIDGSTTFKSATVAAVGDGFIAQ 1076
Query: 144 DI 145
DI
Sbjct: 1077DI 1082
>AV426815
Length = 391
Score = 100 bits (250), Expect = 2e-22
Identities = 51/101 (50%), Positives = 66/101 (64%)
Frame = +3
Query: 31 FPSWVKPGDRKLLQASAVPADAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKKGVYN 90
+P W+ DR+LLQA V AD VVA+DGSGN+ + AV AAP SK RYVI IK GVY
Sbjct: 63 WPEWISAADRRLLQAGTVKADVVVAADGSGNFKTVSAAVAAAPEKSKTRYVIKIKAGVYR 242
Query: 91 EHVMINNSKSNLMMIGDGMGATVITGDLSWGRDKLDTSYTY 131
E+V + K+N+M +GDG T+ITG R+ +D S T+
Sbjct: 243 ENVEVPKKKTNIMFLGDGRTTTIITG----SRNVVDGSTTF 353
>CN825195
Length = 551
Score = 82.8 bits (203), Expect = 6e-17
Identities = 44/113 (38%), Positives = 66/113 (57%), Gaps = 3/113 (2%)
Frame = +2
Query: 31 FPSWVKPGDRKLLQ--ASAVPADAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKKGV 88
FP W+ DR+LL SA+ AD VV+ DG+G I +A+ P +R++I+++ G
Sbjct: 209 FPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGR 388
Query: 89 YNEH-VMINNSKSNLMMIGDGMGATVITGDLSWGRDKLDTSYTYTFGVEGLGF 140
Y E+ + I ++N+M+IGDG G TVITG + L T +T +F G GF
Sbjct: 389 YEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMYGLLTTFHTASFAASGAGF 547
>TC9501 weakly similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase ,
partial (29%)
Length = 934
Score = 80.1 bits (196), Expect = 4e-16
Identities = 51/148 (34%), Positives = 70/148 (46%), Gaps = 1/148 (0%)
Frame = +2
Query: 112 TVITGDLSWGRDKLDTSYTYTFGVEGLGFSAQDISFRNTAWPENHQAVALLSDSDTSVFY 171
T+ITG ++ D + T T TF + GF A+ I+F NTA P HQAVAL + D S F
Sbjct: 14 TIITGRKNFA-DGVKTMQTATFANQAPGFIAKAITFENTAGPNGHQAVALRNQGDQSAFV 190
Query: 172 RCEISGFQDSLCANIKHHSIRIAKSEARLTSYLVRQLSSFKTDILVRKGPTGQQ-NTITA 230
C I G+QD+L R + + + I+V + P Q NT+TA
Sbjct: 191 GCHILGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSPTLIQHSIIVVRKPNDNQFNTVTA 370
Query: 231 QGGPEKPNLPFGFAFQFCNVCADPEFLP 258
G P K N+P G Q C + + P
Sbjct: 371 DGTPTK-NMPTGIVIQDCEIVPEAALFP 451
>BP039243
Length = 462
Score = 73.2 bits (178), Expect = 5e-14
Identities = 34/64 (53%), Positives = 45/64 (70%)
Frame = -2
Query: 51 DAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKKGVYNEHVMINNSKSNLMMIGDGMG 110
D VVA DG+GN+ I +AV AAPN R+VIHIK G Y E+V + K+N+M +GDG+G
Sbjct: 443 DLVVAKDGTGNFAPIGEAVAAAPNSRATRFVIHIKAGAYFENVEVIRKKTNVMFVGDGIG 264
Query: 111 ATVI 114
TV+
Sbjct: 263 KTVV 252
>TC19984 similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase , partial (11%)
Length = 300
Score = 68.6 bits (166), Expect = 1e-12
Identities = 34/80 (42%), Positives = 49/80 (60%), Gaps = 2/80 (2%)
Frame = +3
Query: 31 FPSWVKPGDRKLLQAS--AVPADAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKKGV 88
FPSWV DR+LL+A+ + VA DGSG++ I +A+ P K RYV+++K+GV
Sbjct: 51 FPSWVHVDDRRLLKAADNRPKPNVTVAKDGSGDFKTISEALNKIPATYKGRYVVYVKEGV 230
Query: 89 YNEHVMINNSKSNLMMIGDG 108
Y+E V + N+ M GDG
Sbjct: 231 YDEQVTVTKKMQNVTMYGDG 290
>BP033848
Length = 525
Score = 63.2 bits (152), Expect = 5e-11
Identities = 31/75 (41%), Positives = 44/75 (58%), Gaps = 4/75 (5%)
Frame = +2
Query: 32 PSWVKPGDRKLL----QASAVPADAVVASDGSGNYMKIMDAVMAAPNGSKKRYVIHIKKG 87
PSW DRKLL + V +AVVA DGSG + I A+ + P G K RY+I+++ G
Sbjct: 299 PSWFSGADRKLLGKMKKGGKVTPNAVVAKDGSGKFKTIASAIASYPKGFKGRYIIYVRAG 478
Query: 88 VYNEHVMINNSKSNL 102
VY+E++ + NL
Sbjct: 479 VYDEYITVPKQAVNL 523
>TC10949 similar to UP|PME3_PHAVU (Q43111) Pectinesterase 3 precursor
(Pectin methylesterase 3) (PE 3) , partial (34%)
Length = 773
Score = 52.0 bits (123), Expect = 1e-07
Identities = 36/92 (39%), Positives = 44/92 (47%), Gaps = 1/92 (1%)
Frame = +3
Query: 156 HQAVALLSDSDTSVFYRCEISGFQDSLCANIKHHSIRIAKSEARLTSYLVRQLSSFKT-D 214
HQAVA+ S SD SVFYRC GFQD+L A+ R + S F+
Sbjct: 9 HQAVAMRSGSDRSVFYRCSFDGFQDTLYAHSNRQFYRECDITGTIDFIFGNAASIFQNCK 188
Query: 215 ILVRKGPTGQQNTITAQGGPEKPNLPFGFAFQ 246
I+ R+ Q TITAQ G + PN G Q
Sbjct: 189 IMPRQPMPNQFYTITAQ-GKKDPNQNTGIVIQ 281
>AU089161
Length = 400
Score = 35.4 bits (80), Expect(2) = 3e-05
Identities = 23/56 (41%), Positives = 29/56 (51%)
Frame = +1
Query: 106 GDGMGATVITGDLSWGRDKLDTSYTYTFGVEGLGFSAQDISFRNTAWPENHQAVAL 161
G G TVITG+ ++ D T + TF F A+ + F NTA HQAVAL
Sbjct: 85 GXGPKKTVITGNKNFV-DGYKTMRSATFTTVAPDFMAKSMGFENTAGASKHQAVAL 249
Score = 27.7 bits (60), Expect(2) = 3e-05
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 166 DTSVFYRCEISGFQDSLCAN 185
D S F+ C ISG+QD+L A+
Sbjct: 263 DRSAFFDCAISGYQDTLYAH 322
>AU088824
Length = 421
Score = 42.4 bits (98), Expect = 9e-05
Identities = 23/54 (42%), Positives = 33/54 (60%)
Frame = +1
Query: 100 SNLMMIGDGMGATVITGDLSWGRDKLDTSYTYTFGVEGLGFSAQDISFRNTAWP 153
+N++M GDG T+ITG ++ D + T T TF + GF A+ I+F NTA P
Sbjct: 7 ANILMYGDGPTKTIITGRKNFA-DGVKTMQTATFANQAPGFIAKAITFENTAGP 165
>AV426324
Length = 393
Score = 40.4 bits (93), Expect = 4e-04
Identities = 22/37 (59%), Positives = 23/37 (61%), Gaps = 2/37 (5%)
Frame = +1
Query: 28 KGKFPSWVKPGDRKLLQASA--VPADAVVASDGSGNY 62
K FPSWV DRKLLQAS D V A DGSGN+
Sbjct: 280 KNGFPSWVSNKDRKLLQASVSETKFDLVDAKDGSGNF 390
>AU088938
Length = 641
Score = 35.8 bits (81), Expect = 0.009
Identities = 22/67 (32%), Positives = 37/67 (54%), Gaps = 1/67 (1%)
Frame = +1
Query: 4 SRIFFLFCFFFRTSSSDILDTITQKGKFPSWVKPGDRKLLQA-SAVPADAVVASDGSGNY 62
S++ F F + L I +G +P+W+ DRKL+++ S V +A+VA DG G +
Sbjct: 343 SKVLGAFDFNLHLKPARRLLEIDAEG-YPTWLSFADRKLVESKSNVLPNAIVAKDGXGXF 519
Query: 63 MKIMDAV 69
I +A+
Sbjct: 520 KNITEAI 540
>AV776418
Length = 399
Score = 28.5 bits (62), Expect = 1.4
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = +3
Query: 218 RKGPTGQQNTITAQGGPEKPNLPF 241
R G Q+ I GGPE PNLPF
Sbjct: 174 RSGMPYCQHCIRGPGGPETPNLPF 245
>TC8721
Length = 670
Score = 28.1 bits (61), Expect = 1.8
Identities = 15/33 (45%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -3
Query: 73 PNGSKKRYVIHIKKGVYNEH-VMINNSKSNLMM 104
P GS K++ IK G YN H MI SK L +
Sbjct: 437 PRGSDKKHQFSIKGGTYNSHQFMILTSKQALSL 339
>AV771126
Length = 270
Score = 26.9 bits (58), Expect = 4.0
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +3
Query: 231 QGGPEKPNLPF 241
QGGPE PNLPF
Sbjct: 225 QGGPETPNLPF 257
>AV776199
Length = 441
Score = 26.6 bits (57), Expect = 5.2
Identities = 13/25 (52%), Positives = 15/25 (60%), Gaps = 5/25 (20%)
Frame = +2
Query: 232 GGPEKPNLPFGFAFQF-----CNVC 251
GGPE PNLPF F+ F C +C
Sbjct: 215 GGPETPNLPF-FSSHFPTLNECGIC 286
>TC18302 similar to UP|Q8L6T7 (Q8L6T7) Ring-H2 zinc finger protein,
partial (6%)
Length = 304
Score = 26.2 bits (56), Expect = 6.8
Identities = 15/36 (41%), Positives = 16/36 (43%), Gaps = 1/36 (2%)
Frame = +1
Query: 3 PSRIFFLFCFFFRTSSSDI-LDTITQKGKFPSWVKP 37
PS+ FFL C F T SS L F WV P
Sbjct: 19 PSQTFFLLCIFTPTCSSPFPLQNSEVSECFSIWVSP 126
>TC17888 similar to UP|Q9FJA0 (Q9FJA0) Gb|AAD21756.1, partial (9%)
Length = 487
Score = 25.8 bits (55), Expect = 8.9
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = +2
Query: 178 FQDSLCANIKHHSIRIAKSEARLTSYLVRQ 207
+ DSLC NI+ H+I +S S L+++
Sbjct: 2 YLDSLCGNIRSHTITHVQSHDDKVSLLLKE 91
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,976,892
Number of Sequences: 28460
Number of extensions: 64981
Number of successful extensions: 395
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 385
length of query: 287
length of database: 4,897,600
effective HSP length: 89
effective length of query: 198
effective length of database: 2,364,660
effective search space: 468202680
effective search space used: 468202680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148775.4