BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148758.4 - phase: 0 /pseudo
(618 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12586 weakly similar to PIR|C86410|C86410 protein F3M18.18 [im... 201 2e-52
AV419667 183 6e-47
TC15080 126 1e-29
BP084865 102 1e-22
AV769794 102 2e-22
TC17932 89 2e-18
AV407953 54 3e-17
TC15498 weakly similar to PIR|C86410|C86410 protein F3M18.18 [im... 81 6e-16
TC17979 79 3e-15
AU252041 67 8e-12
BG662361 45 4e-05
AV428425 39 0.003
BP072007 33 0.18
TC19435 similar to UP|Q39036 (Q39036) NADPH-ferrihemoprotein red... 32 0.30
BI420016 31 0.67
CB827528 28 3.3
TC14946 similar to UP|Q9SXS8 (Q9SXS8) Ethylene responsive elemen... 28 4.3
TC19974 weakly similar to UP|O48721 (O48721) Expressed protein, ... 28 5.7
TC10427 similar to UP|Q9AU06 (Q9AU06) NADPH-cytochrome P450 oxyd... 28 5.7
TC15782 homologue to PIR|T44259|T44259 lipoic acid synthase [im... 28 5.7
>TC12586 weakly similar to PIR|C86410|C86410 protein F3M18.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(17%)
Length = 622
Score = 201 bits (512), Expect = 2e-52
Identities = 120/234 (51%), Positives = 146/234 (62%), Gaps = 6/234 (2%)
Frame = +1
Query: 177 KIGGTDIIYAKQQYSSPLQPSDVQKKLKDMADELFRGQAGQNNANDGTFNSKEKFMRDNG 236
K+GGTDIIYAKQQYSSP+QP +V+K LKDMAD F GQAGQ+NA+DG N+KEKFM DNG
Sbjct: 1 KLGGTDIIYAKQQYSSPVQPVEVRKLLKDMADNNFLGQAGQHNAHDGKSNTKEKFMMDNG 180
Query: 237 LGFLDIQAQSYRETEKNTTNVLA------IYPLLVKHSRCRI*SLCARGKVEMGNKISAT 290
L F+ IQ +SY E E + + + + C+ L + M +
Sbjct: 181 LDFMGIQPRSYYEAEVQDIKFMCKRKGGNLKKSMTHNDWCQT-VLSQPDLIAMSFIPITS 357
Query: 291 MSGAKLFCLNLM*YQCHSYQLHLYLVE*MGVDI*LMP*ISIYDTADKPAIEELHQFLEFQ 350
+ G +N Y H+ L+L KP IEELHQFLEFQ
Sbjct: 358 LLGG----VNGSGYLTHAINLYLRY---------------------KPPIEELHQFLEFQ 462
Query: 351 LPRQWAPVFGELALGPDRKKSQSSSSLQFSFMGPKLYVNTSPVVVGMKPVTGLR 404
LPRQWAPVFGELALGP+R KSQ+++SLQFSF+GPKLYVNT+PV VG KPVTGLR
Sbjct: 463 LPRQWAPVFGELALGPER-KSQNTASLQFSFLGPKLYVNTTPVDVGKKPVTGLR 621
>AV419667
Length = 406
Score = 183 bits (465), Expect = 6e-47
Identities = 95/120 (79%), Positives = 102/120 (84%)
Frame = +1
Query: 385 KLYVNTSPVVVGMKPVTGLRLYLEGKKSNCLAIHLQHLSSLPKTFQLKDETNRNVSDASS 444
+L +N V VG KPVTGLRLYLEGKK+NCLAIHLQHLSSLPKTFQL DE N NV SS
Sbjct: 46 RLTLNYLQVDVGKKPVTGLRLYLEGKKNNCLAIHLQHLSSLPKTFQLMDEPNGNVPH-SS 222
Query: 445 ERKYYEKVQWKSFSHICTAPVESYDDNAVVTGAHFEVGETGLKKVLFLRLHFCKVADATR 504
ERKYYEKVQWKSFSH+CTAPVES +N+VVTGA F VGE+GLKKVLFLRLHF KVA ATR
Sbjct: 223 ERKYYEKVQWKSFSHVCTAPVESDGENSVVTGARF*VGESGLKKVLFLRLHFSKVAGATR 402
>TC15080
Length = 716
Score = 126 bits (316), Expect = 1e-29
Identities = 62/69 (89%), Positives = 64/69 (91%)
Frame = +1
Query: 549 SALYPGGPPVPAQAPKLLKFVDTTEMTRGPQDLPGYWVVSGARLYVEKGKISLKVKYSLL 608
SALYPGGPPV AQ+ KLL+FVDTTEMTRGPQD PGYWVVSGARLYVEKGKISLKVKYSL
Sbjct: 1 SALYPGGPPVXAQSTKLLRFVDTTEMTRGPQDSPGYWVVSGARLYVEKGKISLKVKYSLK 180
Query: 609 TVILQDEET 617
TVIL DEET
Sbjct: 181 TVILPDEET 207
>BP084865
Length = 484
Score = 102 bits (255), Expect = 1e-22
Identities = 53/108 (49%), Positives = 66/108 (61%)
Frame = -3
Query: 508 PEWDGSPGLTQKSGMISTFISTRFSGPQKLPPPQPSDVNVNSALYPGGPPVPAQAPKLLK 567
PEWD S L Q +I ++ +K P+ +V + SA YP GPP Q P LL+
Sbjct: 479 PEWDRSSNLGQLRNII---FASSVHEKEKKYTPKGGEVTIGSAAYPTGPPSRVQTPSLLR 309
Query: 568 FVDTTEMTRGPQDLPGYWVVSGARLYVEKGKISLKVKYSLLTVILQDE 615
FVDT E+ RGP+ PGYWVVSGAR V+ GKI L VKYSLL ++Q E
Sbjct: 308 FVDTAEIIRGPEHTPGYWVVSGARFSVQNGKIYLSVKYSLLNFVVQCE 165
>AV769794
Length = 559
Score = 102 bits (253), Expect = 2e-22
Identities = 53/120 (44%), Positives = 78/120 (64%), Gaps = 3/120 (2%)
Frame = -2
Query: 492 LRLHFCKVADATRVRAPEWDGSPGLTQKSGMISTFISTRFSGPQKLP---PPQPSDVNVN 548
LRL F + + + +R EW +P ++KS + T +ST FS Q+ PP+ + +N
Sbjct: 555 LRLLFTNIPNCS-IRKTEWAAAPEASRKSSFL-TNLSTTFSFTQQSATSGPPKQAPAMLN 382
Query: 549 SALYPGGPPVPAQAPKLLKFVDTTEMTRGPQDLPGYWVVSGARLYVEKGKISLKVKYSLL 608
S +YP GPPVP +A KLLK+V+ E+ RGP D PG+W+V+ A+L E GKI L+VK++LL
Sbjct: 381 SGIYPDGPPVPVRAGKLLKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKIGLQVKFALL 202
>TC17932
Length = 572
Score = 89.4 bits (220), Expect = 2e-18
Identities = 41/51 (80%), Positives = 48/51 (93%)
Frame = +1
Query: 566 LKFVDTTEMTRGPQDLPGYWVVSGARLYVEKGKISLKVKYSLLTVILQDEE 616
LKFVDTTEM+RGPQ+ PGYWVVSGARL VEKG++SL+VKYSLLT++L DEE
Sbjct: 1 LKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVSLRVKYSLLTMVLPDEE 153
>AV407953
Length = 416
Score = 53.9 bits (128), Expect(3) = 3e-17
Identities = 31/56 (55%), Positives = 36/56 (63%)
Frame = +2
Query: 278 RGKVEMGNKISATMSGAKLFCLNLM*YQCHSYQLHLYLVE*MGVDI*LMP*ISIYD 333
RG E+ I MSG +LF +LM* QC S+QLHLY +E GVD * MP* IYD
Sbjct: 203 RGGAEVMTGICPIMSGCRLFRWSLM*SQCPSFQLHLY*MECQGVDS*AMP*TFIYD 370
Score = 40.4 bits (93), Expect(3) = 3e-17
Identities = 27/70 (38%), Positives = 42/70 (59%), Gaps = 2/70 (2%)
Frame = +1
Query: 194 LQPSDVQKKLKDMADELFRGQAGQNN-ANDGTFNSKEKFMRDNGLGFLDIQ-AQSYRETE 251
LQP+DVQK+LK+MAD F GQ + A+D F + + +R+ L F +I + SY E
Sbjct: 4 LQPADVQKRLKEMADRRFMDANGQYSVASDQVFPNDKFGVREQRLTFANISPSSSYSHKE 183
Query: 252 KNTTNVLAIY 261
++++IY
Sbjct: 184 ----DIVSIY 201
Score = 30.8 bits (68), Expect(3) = 3e-17
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +1
Query: 337 KPAIEELHQFLEFQ 350
KP IEELHQFLEFQ
Sbjct: 373 KPPIEELHQFLEFQ 414
>TC15498 weakly similar to PIR|C86410|C86410 protein F3M18.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(7%)
Length = 515
Score = 80.9 bits (198), Expect = 6e-16
Identities = 51/124 (41%), Positives = 68/124 (54%)
Frame = +3
Query: 485 GLKKVLFLRLHFCKVADATRVRAPEWDGSPGLTQKSGMISTFISTRFSGPQKLPPPQPSD 544
G + VL L+L F KV T +R WD +P + S S+ L D
Sbjct: 12 GAQNVLHLKLLFSKVPGCT-IRRSVWDHNPSTPATAAQRSDGASS------SLTKKTSDD 170
Query: 545 VNVNSALYPGGPPVPAQAPKLLKFVDTTEMTRGPQDLPGYWVVSGARLYVEKGKISLKVK 604
+S+++ G KL K VD TEM RGPQD+PG+W+V+GA+L VEKGKI L++K
Sbjct: 171 KKEDSSIHIG---------KLAKIVDMTEMFRGPQDIPGHWLVTGAKLGVEKGKIVLRIK 323
Query: 605 YSLL 608
YSLL
Sbjct: 324 YSLL 335
>TC17979
Length = 777
Score = 78.6 bits (192), Expect = 3e-15
Identities = 47/136 (34%), Positives = 73/136 (53%)
Frame = +3
Query: 473 VVTGAHFEVGETGLKKVLFLRLHFCKVADATRVRAPEWDGSPGLTQKSGMISTFISTRFS 532
VVTGA V + G + VL+++L + ++ T +R WD P + + +
Sbjct: 9 VVTGAQLGVWDFGSRNVLYMKLLYSRLPGCT-IRRSLWDHIPNQPLPTVSVGNTSN---- 173
Query: 533 GPQKLPPPQPSDVNVNSALYPGGPPVPAQAPKLLKFVDTTEMTRGPQDLPGYWVVSGARL 592
P S ++ PG +L+K+VD +EM++GPQD PG+W+V+G +L
Sbjct: 174 -------PDESSISFRENTTPGN--------ELVKYVDLSEMSKGPQDPPGHWLVTGGKL 308
Query: 593 YVEKGKISLKVKYSLL 608
VEKGKI L++KYSLL
Sbjct: 309 GVEKGKIVLRLKYSLL 356
>AU252041
Length = 326
Score = 67.0 bits (162), Expect = 8e-12
Identities = 33/93 (35%), Positives = 56/93 (59%), Gaps = 2/93 (2%)
Frame = +3
Query: 14 IKSVGLGYDLTNDLRLKFCKY--DSKLIAIDHDNLRTVELPGRVSIPNVPKSINCDKGDR 71
I+++G G+D+T+D+RL +CK S+L+ D ++ R + + V +PNV I C G
Sbjct: 48 IQALGRGFDVTSDIRLLYCKGAPGSRLVRFDEEHTRDLAISHGVVVPNVSGDIVCSLGKS 227
Query: 72 MRLCSDVLSFQQMSEQFNQEVSLSGKIPTGHFN 104
+ V SF +M++ FN+ ++G+IP G FN
Sbjct: 228 ALEKTPVCSFHEMAKHFNERSGITGQIPLGSFN 326
>BG662361
Length = 330
Score = 44.7 bits (104), Expect = 4e-05
Identities = 30/73 (41%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = +1
Query: 336 DKPAIEELHQFLEFQLPRQWAPVFGELALGPDRKKSQSSSSLQFSFMGPKLYVNT-SPVV 394
DKP IE+L FL+FQ+ R WAP L + K S G + + V
Sbjct: 127 DKPPIEDLQYFLDFQITRVWAPEQNNL-----QTKGACLSIPSVQLDGT*AFCSVPDQVT 291
Query: 395 VGMKPVTGLRLYL 407
VG KPVTGLRL L
Sbjct: 292 VGRKPVTGLRLSL 330
>AV428425
Length = 422
Score = 38.5 bits (88), Expect = 0.003
Identities = 21/46 (45%), Positives = 26/46 (55%)
Frame = +3
Query: 337 KPAIEELHQFLEFQLPRQWAPVFGELALGPDRKKSQSSSSLQFSFM 382
KP IE+L FL+FQ+ R WAP L ++K SLQFS M
Sbjct: 300 KPPIEDLQYFLDFQITRVWAPEQNNL-----QRKEPVCPSLQFSLM 422
>BP072007
Length = 362
Score = 32.7 bits (73), Expect = 0.18
Identities = 19/35 (54%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = -3
Query: 576 RGPQDL--PGYWVVSGARLYVEKGKISLKVKYSLL 608
RGP L + V +G L VEKGKI L++KYSLL
Sbjct: 264 RGPPRLFLVTWLVTTGLELGVEKGKIVLRIKYSLL 160
>TC19435 similar to UP|Q39036 (Q39036) NADPH-ferrihemoprotein reductase ,
partial (12%)
Length = 459
Score = 32.0 bits (71), Expect = 0.30
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 2/57 (3%)
Frame = +3
Query: 528 STRFSGPQKLPPPQPSDVNVNSALYPGGPPVPAQAPKLLKFVDTT--EMTRGPQDLP 582
S+R S PPP PS +S GPPV +P L +T +T P+ P
Sbjct: 186 SSRMSSSWSSPPPSPSSSAASSFSSGAGPPVTMLSPSSLPSASSTRLRLTTAPKRSP 356
>BI420016
Length = 559
Score = 30.8 bits (68), Expect = 0.67
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = +2
Query: 532 SGPQKLPPPQPSDVNVNSALYPGGPPVPAQAP 563
S P PPP P N+ L P PP P P
Sbjct: 251 SSPTLTPPPSPPSPTPNAPLSPSSPPSPPTPP 346
>CB827528
Length = 510
Score = 28.5 bits (62), Expect = 3.3
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 6/36 (16%)
Frame = +1
Query: 534 PQKLPPPQPSDVNVNSALYP------GGPPVPAQAP 563
P LPPP+P+ VN + + +P PP Q+P
Sbjct: 109 PTALPPPKPTSVNPSPSHFPPLDQNRASPPCSLQSP 216
>TC14946 similar to UP|Q9SXS8 (Q9SXS8) Ethylene responsive element binding
factor, partial (26%)
Length = 631
Score = 28.1 bits (61), Expect = 4.3
Identities = 18/39 (46%), Positives = 20/39 (51%)
Frame = +3
Query: 520 SGMISTFISTRFSGPQKLPPPQPSDVNVNSALYPGGPPV 558
SGM ST S FSGP+ + PP P YP PPV
Sbjct: 78 SGMSSTVES--FSGPRPVRPPAPPVAAARR--YPRSPPV 182
>TC19974 weakly similar to UP|O48721 (O48721) Expressed protein, partial
(29%)
Length = 444
Score = 27.7 bits (60), Expect = 5.7
Identities = 12/41 (29%), Positives = 23/41 (55%)
Frame = +1
Query: 421 HLSSLPKTFQLKDETNRNVSDASSERKYYEKVQWKSFSHIC 461
+L +L +TF + T N+S S+ R + + ++ SH+C
Sbjct: 31 YLFALSRTFFVSSSTPPNLSRFSTHRNRFHRHRFHRHSHVC 153
>TC10427 similar to UP|Q9AU06 (Q9AU06) NADPH-cytochrome P450 oxydoreductase
isoform 3, partial (17%)
Length = 565
Score = 27.7 bits (60), Expect = 5.7
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 537 LPPPQPSDVNVNSALYPGGPPVPAQAPKLL 566
LPPP PS + +S + PPV +P L
Sbjct: 268 LPPPSPSSSDASSFSFGADPPVTRLSPSSL 357
>TC15782 homologue to PIR|T44259|T44259 lipoic acid synthase [imported] -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (38%)
Length = 544
Score = 27.7 bits (60), Expect = 5.7
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 538 PPPQPSDVNVNSALYPGGPPVPAQAPKLLK 567
P P+PS+ N +L P PP P P+ K
Sbjct: 18 PSPEPSNGPPNPSLQPQSPPPPPTPPRRRK 107
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,874,001
Number of Sequences: 28460
Number of extensions: 133360
Number of successful extensions: 750
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of query: 618
length of database: 4,897,600
effective HSP length: 96
effective length of query: 522
effective length of database: 2,165,440
effective search space: 1130359680
effective search space used: 1130359680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148758.4