BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.4 - phase: 0
(427 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP040145 276 6e-75
TC15298 homologue to UP|QUA1_ARATH (Q9LSG3) Glycosyltransferase ... 230 3e-61
BU494183 158 1e-39
AU089457 145 1e-35
TC12928 126 6e-30
TC9237 weakly similar to UP|GLTR_ARATH (Q9FWA4) Probable glycosy... 99 1e-21
TC19099 similar to UP|QUA1_ARATH (Q9LSG3) Glycosyltransferase QU... 93 1e-19
TC10723 similar to GB|AAB61117.1|2194142|F20P5 ESTs gb|N38288,gb... 91 4e-19
TC19756 similar to UP|GLTR_ARATH (Q9FWA4) Probable glycosyltrans... 84 5e-18
AU088971 75 2e-14
TC10573 similar to UP|Q9FIK3 (Q9FIK3) Emb|CAB71043.1, partial (10%) 71 3e-13
AV775940 60 1e-10
AV769006 44 5e-05
AV426189 40 0.001
BI418492 38 0.003
TC15131 similar to UP|Q9FX71 (Q9FX71) T6J4.1 protein, partial (40%) 33 0.069
TC10761 similar to UP|Q9SXL7 (Q9SXL7) Csf-3 protein, partial (47%) 30 0.99
TC16973 weakly similar to UP|Q96477 (Q96477) LRR protein, partia... 28 2.2
TC12123 similar to GB|AAO39931.1|28372898|BT003703 At4g28730 {Ar... 28 2.9
TC17672 similar to UP|Q93X83 (Q93X83) Hsp70 interacting protein/... 27 6.4
>BP040145
Length = 530
Score = 276 bits (705), Expect = 6e-75
Identities = 120/176 (68%), Positives = 149/176 (84%)
Frame = +1
Query: 215 ALSPTYSSVMNHIRIYIPELFPSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCK 274
A SP Y S++NH+RIYIPELFP+LDKVVFLD+D+V+Q DLSPLW+ID+ GKVNGAVETCK
Sbjct: 1 ARSPKYISLLNHLRIYIPELFPNLDKVVFLDDDVVIQRDLSPLWEIDLEGKVNGAVETCK 180
Query: 275 GKDKRVMSKMLKSYLNFSHPLISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQ 334
G+D+ VMSK ++Y NFSHPL++++ DP ECAWAYGMNI DL AWR+TNI TYH W+++
Sbjct: 181 GEDEWVMSKRFRNYFNFSHPLVAEHLDPEECAWAYGMNIFDLSAWRRTNIRETYHSWLKE 360
Query: 335 NIKSDLSLWHLGTLPPGLIAFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHF 390
N+KS+L++W LGTLPP LIAF G+VH IDP WHMLGLGYQ NTN+ K A V+H+
Sbjct: 361 NLKSNLTMWKLGTLPPALIAFKGHVHPIDPSWHMLGLGYQNNTNIERVKKAAVIHY 528
>TC15298 homologue to UP|QUA1_ARATH (Q9LSG3) Glycosyltransferase QUASIMODO1
, partial (33%)
Length = 818
Score = 230 bits (587), Expect = 3e-61
Identities = 105/189 (55%), Positives = 131/189 (68%)
Frame = +2
Query: 236 PSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSHPL 295
P L K++FLD+DIVVQ DL+ LW IDM+GKVNGAVETC G R Y+NFSHPL
Sbjct: 2 PKLHKILFLDDDIVVQKDLTGLWKIDMDGKVNGAVETCFGSFHRYAQ-----YMNFSHPL 166
Query: 296 ISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPGLIAF 355
I F+P CAWAYGMN DL+AWR+ + YHYW QN+ + +LW LGTLPPGLI +
Sbjct: 167 IKAKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYW--QNLNENRTLWKLGTLPPGLITY 340
Query: 356 HGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTKYVDF 415
+ +D WH+LGLGY + ++ + KNA VVHFNG KPWLDIA Q KPLW KYVD+
Sbjct: 341 YATTKPLDKSWHVLGLGYNPSISMDEIKNAAVVHFNGNMKPWLDIAMTQFKPLWAKYVDY 520
Query: 416 SDNFIESCH 424
F+++C+
Sbjct: 521 ELEFVQACN 547
>BU494183
Length = 460
Score = 158 bits (400), Expect = 1e-39
Identities = 76/142 (53%), Positives = 91/142 (63%)
Frame = +2
Query: 237 SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSHPLI 296
S V+FLD+DIVVQ DL+ LW D+ G VNGAVETC R YLNFS+PLI
Sbjct: 17 SSTNVLFLDDDIVVQKDLTGLWSTDLKGNVNGAVETCGESFHR-----FDRYLNFSNPLI 181
Query: 297 SKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPGLIAFH 356
+KNFDP C WAYGMN+ DL W++ NI+ YH W Q + D LW LGTLPPGLI F
Sbjct: 182 AKNFDPPACGWAYGMNVFDLVEWKRQNITEVYHNW--QKLNHDRQLWKLGTLPPGLITFW 355
Query: 357 GNVHTIDPFWHMLGLGYQENTN 378
++ +WH+LGLGY N N
Sbjct: 356 KRTFPLNRYWHVLGLGYNPNVN 421
>AU089457
Length = 505
Score = 145 bits (366), Expect = 1e-35
Identities = 64/76 (84%), Positives = 70/76 (91%)
Frame = +1
Query: 352 LIAFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTK 411
LIAFHG VHTIDPFWHMLGLGYQENT+ DA++A V+HFNG AKPWLDIAF QL+PLWTK
Sbjct: 10 LIAFHGYVHTIDPFWHMLGLGYQENTSFADAESAAVIHFNGRAKPWLDIAFSQLRPLWTK 189
Query: 412 YVDFSDNFIESCHIRA 427
YVDFSDNFI+SCHIRA
Sbjct: 190 YVDFSDNFIKSCHIRA 237
>TC12928
Length = 755
Score = 126 bits (317), Expect = 6e-30
Identities = 54/140 (38%), Positives = 88/140 (62%)
Frame = +3
Query: 288 YLNFSHPLISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGT 347
++NFS P I+K FD N C WA+GMN+ DL+ W++ +++ YH ++E K + L + G+
Sbjct: 45 FINFSDPFIAKRFDVNACTWAFGMNLFDLQQWKRHDLTAVYHKYLEMGAK--MPLLNSGS 218
Query: 348 LPPGLIAFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKP 407
LP G + F+ +D WH+LGLGY + + + A V+H++G KPWLDIA + K
Sbjct: 219 LPLGWLTFYEKTLILDRRWHILGLGYDSIVDTNEIERAAVIHYDGIRKPWLDIAVGRYKS 398
Query: 408 LWTKYVDFSDNFIESCHIRA 427
WTK++ F ++ C+++A
Sbjct: 399 YWTKFMKFDLPILQRCNLQA 458
>TC9237 weakly similar to UP|GLTR_ARATH (Q9FWA4) Probable
glycosyltransferase At3g02350 , partial (4%)
Length = 912
Score = 99.0 bits (245), Expect = 1e-21
Identities = 48/144 (33%), Positives = 73/144 (50%), Gaps = 1/144 (0%)
Frame = +1
Query: 285 LKSYLNFSHPLISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWV-EQNIKSDLSLW 343
LKSYL K F N CAW G+NI+DL WR+ ++ TY + E NI+ W
Sbjct: 1 LKSYLG------EKGFSHNSCAWMSGLNIIDLVRWRELGLTQTYRRLIKEMNIQEGSGAW 162
Query: 344 HLGTLPPGLIAFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFP 403
L+AF ++ +D W + GLG+ + K + V+H+NG KPWLD+ P
Sbjct: 163 RAS-----LLAFENKIYPLDKSWVLSGLGHDYTIDTHAIKTSPVLHYNGKMKPWLDLGIP 327
Query: 404 QLKPLWTKYVDFSDNFIESCHIRA 427
K W K+++ D + C++ +
Sbjct: 328 NYKSSWKKFLNKEDQLLSECNVNS 399
>TC19099 similar to UP|QUA1_ARATH (Q9LSG3) Glycosyltransferase QUASIMODO1 ,
partial (35%)
Length = 597
Score = 92.8 bits (229), Expect = 1e-19
Identities = 49/128 (38%), Positives = 73/128 (56%)
Frame = +2
Query: 55 SNIASNSIPEQLHCLDLTLANEHANNAAARLRLPSADLVPALVDNSYFHFVLASDNVLAA 114
S IA+ SIP+ LHCL + L E +A + P + D + +H+ L SDNV+AA
Sbjct: 173 SLIAAKSIPKSLHCLSMRLMEERIAHAE-KYSDEGKPTPPEVEDPNLYHYALFSDNVVAA 349
Query: 115 SVVAKSLVQNSLQPWKVVLHIITDKKTYNSMQAWFSLHSLSPAIIEVKSLQDFDWFREGK 174
SVV S +N+ +PWK V H++TDK +MQ F L + A IEVK+++D+ +
Sbjct: 350 SVVVNSATKNAQEPWKHVFHVVTDKMNLGAMQVMFKLKDYNGAHIEVKAVEDYKFLNSSY 529
Query: 175 VSLLEAME 182
V +L +E
Sbjct: 530 VPVLRQLE 553
>TC10723 similar to GB|AAB61117.1|2194142|F20P5 ESTs
gb|N38288,gb|T43486,gb|AA395242 come from this gene.
{Arabidopsis thaliana;}, partial (49%)
Length = 1008
Score = 90.9 bits (224), Expect = 4e-19
Identities = 58/187 (31%), Positives = 95/187 (50%), Gaps = 8/187 (4%)
Frame = +1
Query: 234 LFPSLDKVVFLDNDIVVQTDLSPLWDIDM-NGKVNGAVETCKGKDKRVMSKMLKSYLNFS 292
L P +D+V++LD+D+VV D+S LW++ + +V GA E C + + +
Sbjct: 22 LDPCVDRVIYLDSDLVVVDDVSKLWNVTLAESRVIGAPEYCHANFTKYFTDEF-----WV 186
Query: 293 HPLISKNFDPNE-CAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPG 351
P++S+ F C + G+ ++DL WR+ N W+E K ++ LG+LPP
Sbjct: 187 DPVLSRVFSSRRPCYFNTGVMVMDLVRWREGNYRKRIENWMELQRKK--RIYELGSLPPF 360
Query: 352 LIAFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAG---VVHFNGWAKPWLDIAFPQ---L 405
L+ F GNV ID W+ GLG + + + G ++H++G KPW+ + Q L
Sbjct: 361 LLVFAGNVEAIDHRWNQHGLGGDNLNGVCRSLHPGPVSLLHWSGKGKPWVRLDNKQPCPL 540
Query: 406 KPLWTKY 412
LW Y
Sbjct: 541 DRLWEPY 561
>TC19756 similar to UP|GLTR_ARATH (Q9FWA4) Probable glycosyltransferase
At3g02350 , partial (18%)
Length = 396
Score = 84.0 bits (206), Expect(2) = 5e-18
Identities = 41/111 (36%), Positives = 69/111 (61%)
Frame = +3
Query: 159 IEVKSLQDFDWFREGKVSLLEAMEKDQHVRSRFRGGSSAIISNTAEKPEDIAAKLKALSP 218
+E+K+L++F + V +L +E + + R+ N AE D A +K S
Sbjct: 87 LEIKTLEEFTFLNSSYVPVLRQLESAK-IHQRY-------FENPAENGTDDAHDMKFKSA 242
Query: 219 TYSSVMNHIRIYIPELFPSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGA 269
Y S+++H+R Y+P+++P+L ++ LD+D+VVQ DL+ LW ID++GKVNGA
Sbjct: 243 KYLSMLDHLRFYLPQMYPNLHHILLLDDDVVVQKDLTGLWKIDLDGKVNGA 395
Score = 23.5 bits (49), Expect(2) = 5e-18
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +2
Query: 132 VLHIITDKKTYNSMQAWFSL 151
V HI+T++ +M+ WF +
Sbjct: 2 VFHIVTNRMNVAAMKVWFKI 61
>AU088971
Length = 218
Score = 75.5 bits (184), Expect = 2e-14
Identities = 32/69 (46%), Positives = 44/69 (63%)
Frame = +3
Query: 310 GMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPGLIAFHGNVHTIDPFWHML 369
GMN+ DL+ W++ +I+ YH W N+ LW LGTLPPGL+ F+G H + WH+L
Sbjct: 6 GMNMFDLKVWKQKDITGIYHKWX--NMNEXXVLWKLGTLPPGLMTFYGMTHPLXXSWHVL 179
Query: 370 GLGYQENTN 378
GLGY N +
Sbjct: 180 GLGYNPNVD 206
>TC10573 similar to UP|Q9FIK3 (Q9FIK3) Emb|CAB71043.1, partial (10%)
Length = 605
Score = 71.2 bits (173), Expect = 3e-13
Identities = 26/60 (43%), Positives = 40/60 (66%)
Frame = +2
Query: 366 WHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTKYVDFSDNFIESCHI 425
WH+LGLGY N N D + + V+H+NG KPWL+I+ P+ + W KYV++ ++ C+I
Sbjct: 5 WHVLGLGYNTNVNPKDIERSAVIHYNGNLKPWLEISIPKFRSYWVKYVNYDHAYMRECNI 184
>AV775940
Length = 341
Score = 59.7 bits (143), Expect(2) = 1e-10
Identities = 24/47 (51%), Positives = 33/47 (70%)
Frame = -2
Query: 377 TNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTKYVDFSDNFIESC 423
+ L + KNA VVH +G KPWLD+A Q +PLW KYVD+ F+++C
Sbjct: 301 SRLDEIKNAAVVHVHGNMKPWLDLAMTQFQPLWAKYVDYELEFVQAC 161
Score = 22.7 bits (47), Expect(2) = 1e-10
Identities = 7/8 (87%), Positives = 8/8 (99%)
Frame = -1
Query: 366 WHMLGLGY 373
WH+LGLGY
Sbjct: 335 WHVLGLGY 312
>AV769006
Length = 286
Score = 43.9 bits (102), Expect = 5e-05
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = -2
Query: 387 VVHFNGWAKPWLDIAFPQLKPLWTKYVDFSDNFIESCHI 425
VV +NG KPWLDIA + + WTKYV ++ ++ +C +
Sbjct: 285 VVLYNGNMKPWLDIAMQKYRRYWTKYVKYNHPYLRNCKL 169
>AV426189
Length = 408
Score = 39.7 bits (91), Expect = 0.001
Identities = 17/38 (44%), Positives = 29/38 (75%), Gaps = 1/38 (2%)
Frame = +2
Query: 229 IYIPELFP-SLDKVVFLDNDIVVQTDLSPLWDIDMNGK 265
+++ +FP SL+KV+F+D D VV+ D+ L+D+D+ GK
Sbjct: 182 LFLDVIFPLSLEKVIFVDADQVVRADMGELYDMDLKGK 295
>BI418492
Length = 537
Score = 38.1 bits (87), Expect = 0.003
Identities = 17/46 (36%), Positives = 29/46 (62%), Gaps = 1/46 (2%)
Frame = +3
Query: 224 MNHIRIYIPELFPS-LDKVVFLDNDIVVQTDLSPLWDIDMNGKVNG 268
+N+ R Y+ +L S + +V++LD+D+VV D+S LW + K G
Sbjct: 399 LNYARNYLADLLESCVGRVIYLDSDLVVVDDISKLWSTSLGSKTIG 536
>TC15131 similar to UP|Q9FX71 (Q9FX71) T6J4.1 protein, partial (40%)
Length = 553
Score = 33.5 bits (75), Expect = 0.069
Identities = 11/36 (30%), Positives = 27/36 (74%), Gaps = 1/36 (2%)
Frame = +1
Query: 224 MNHIRIYIPELFPS-LDKVVFLDNDIVVQTDLSPLW 258
+N+ R+Y+ +L P+ + ++++ D+D++V D++ LW
Sbjct: 424 LNYARMYLADLVPATVRRIIYFDSDLIVVDDVAKLW 531
>TC10761 similar to UP|Q9SXL7 (Q9SXL7) Csf-3 protein, partial (47%)
Length = 554
Score = 29.6 bits (65), Expect = 0.99
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -3
Query: 43 NAVDCRNGENKCSNIASNSIPEQLHCLDL--TLANEHANNAA 82
N + NG+NKCSN+ +N + H L N++ N+AA
Sbjct: 489 NLIQSENGKNKCSNLKANQQKQNHHRLTRW*KETNKYLNSAA 364
>TC16973 weakly similar to UP|Q96477 (Q96477) LRR protein, partial (30%)
Length = 555
Score = 28.5 bits (62), Expect = 2.2
Identities = 22/87 (25%), Positives = 40/87 (45%)
Frame = -1
Query: 90 ADLVPALVDNSYFHFVLASDNVLAASVVAKSLVQNSLQPWKVVLHIITDKKTYNSMQAWF 149
+D V ALV H L S K + + Q W V L I KT+ ++
Sbjct: 330 SDTVSALV*RKPLHSSLRMIGEFLLSSAPKIIRNQTFQLWAVELVIF---KTFRWERSEV 160
Query: 150 SLHSLSPAIIEVKSLQDFDWFREGKVS 176
+++ S + V++++DF+ F+ ++S
Sbjct: 159 AIYWYSSTEVIVRNIKDFEIFKMSELS 79
>TC12123 similar to GB|AAO39931.1|28372898|BT003703 At4g28730 {Arabidopsis
thaliana;}, partial (47%)
Length = 511
Score = 28.1 bits (61), Expect = 2.9
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = -2
Query: 404 QLKPLWTKYVDFSDNFI 420
QL+PLWT +V F D +I
Sbjct: 456 QLRPLWTHFVQFEDQWI 406
>TC17672 similar to UP|Q93X83 (Q93X83) Hsp70 interacting protein/thioredoxin
chimera, partial (33%)
Length = 709
Score = 26.9 bits (58), Expect = 6.4
Identities = 29/122 (23%), Positives = 44/122 (35%), Gaps = 15/122 (12%)
Frame = +1
Query: 179 EAMEKDQHVRSRFRGGSSAIISNTAEKPEDIAAKLKA-------LSPTYSSVMNHIRIYI 231
+A +DQ S + G I + E + A K + T+ I
Sbjct: 40 QAEAQDQEALSALKDGQVISIHSGGELETKLGAASKTSRLAILYFTATWCGPCRFISPIY 219
Query: 232 PELFPSLDKVVFLDNDIVVQTDLSPLWDID--------MNGKVNGAVETCKGKDKRVMSK 283
L KVVFL DI D++ W+I NGK V++ G DK + +
Sbjct: 220 TSLAEKYPKVVFLKVDIDEARDVAARWNISSVPSFFFVKNGK---EVDSAVGADKSTLER 390
Query: 284 ML 285
+
Sbjct: 391 KI 396
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,289,203
Number of Sequences: 28460
Number of extensions: 120826
Number of successful extensions: 649
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of query: 427
length of database: 4,897,600
effective HSP length: 93
effective length of query: 334
effective length of database: 2,250,820
effective search space: 751773880
effective search space used: 751773880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148653.4