BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.2 + phase: 0
(117 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8536 weakly similar to UP|AAR92224 (AAR92224) Cystatin, partia... 134 3e-33
TC15594 similar to UP|CYTI_VIGUN (Q06445) Cysteine proteinase in... 69 2e-13
TC8168 similar to UP|O04720 (O04720) Cysteine proteinase inhibit... 68 5e-13
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 27 0.90
TC11782 similar to UP|Q9LUV6 (Q9LUV6) Genomic DNA, chromosome 3,... 26 1.5
BI419979 25 3.4
TC11370 similar to UP|Q8L539 (Q8L539) 5-formyltetrahydrofolate c... 24 5.8
BP048729 24 7.6
TC14607 24 7.6
TC17291 similar to UP|Q8LG11 (Q8LG11) Proline-rich cell wall pro... 23 9.9
AV418977 23 9.9
TC10393 23 9.9
AV428920 23 9.9
>TC8536 weakly similar to UP|AAR92224 (AAR92224) Cystatin, partial (69%)
Length = 543
Score = 134 bits (338), Expect = 3e-33
Identities = 67/112 (59%), Positives = 86/112 (75%), Gaps = 3/112 (2%)
Frame = +1
Query: 8 LVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVI 67
L++L V FS A + GG++PI D+NDPHV EIANFAV+EYD++SGAKL+ KV+
Sbjct: 82 LLILSAVSGFSAALRQKGAFVGGYTPIKDLNDPHVKEIANFAVSEYDKQSGAKLRLVKVV 261
Query: 68 NGESQVVAGTNYRLTLSASDGSYS---KNYEAVVWEKIWQHFRNLTSFVPVH 116
+G+SQVVAGTNYRL ++A GS S NYEA+V+E+ W+HFRNLTSF PVH
Sbjct: 262 SGDSQVVAGTNYRLVIAAKGGSRSAAASNYEALVYERSWEHFRNLTSFKPVH 417
>TC15594 similar to UP|CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor
(Cystatin), complete
Length = 703
Score = 68.9 bits (167), Expect = 2e-13
Identities = 34/87 (39%), Positives = 52/87 (59%), Gaps = 1/87 (1%)
Frame = +2
Query: 27 APGGWSPID-DINDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSA 85
A GG S + + N + +A FAV E++++ L+F KV++ QVVAGT Y++ L
Sbjct: 170 AVGGASEVPGNQNSLEIENLARFAVEEHNKKQNTLLEFGKVVSATQQVVAGTLYKIKLEV 349
Query: 86 SDGSYSKNYEAVVWEKIWQHFRNLTSF 112
+DG + YEA +WEK W +F+ L F
Sbjct: 350 NDGGKKQVYEAKIWEKPWLNFKELQEF 430
>TC8168 similar to UP|O04720 (O04720) Cysteine proteinase inhibitor,
partial (55%)
Length = 808
Score = 67.8 bits (164), Expect = 5e-13
Identities = 33/78 (42%), Positives = 46/78 (58%)
Frame = +1
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E++ + A L+F +V+ + QVVAG+ + LTL A DG K YEA
Sbjct: 349 NSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAK 528
Query: 98 VWEKIWQHFRNLTSFVPV 115
VW K W +F+ L F V
Sbjct: 529 VWVKSWLNFKELQEFKEV 582
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 26.9 bits (58), Expect = 0.90
Identities = 16/69 (23%), Positives = 31/69 (44%), Gaps = 3/69 (4%)
Frame = -2
Query: 6 LLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKL---K 62
+ ++ LF+++ F + N+ W + +N+P + AV E+D K+
Sbjct: 293 IFIIFLFIIIFFFFIVGLNRLGRAIWVNLRIMNEPSLK-----AVMEFDEAVKTKVVR*L 129
Query: 63 FEKVINGES 71
V+ GES
Sbjct: 128 DNHVVGGES 102
>TC11782 similar to UP|Q9LUV6 (Q9LUV6) Genomic DNA, chromosome 3, P1 clone:
MCB17, partial (12%)
Length = 468
Score = 26.2 bits (56), Expect = 1.5
Identities = 16/42 (38%), Positives = 20/42 (47%)
Frame = -3
Query: 66 VINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFR 107
VI GES+V A + DGS ++ EAV W W R
Sbjct: 445 VIRGESEVGADA----AVGEDDGSALEHVEAVGWRGWWAALR 332
>BI419979
Length = 495
Score = 25.0 bits (53), Expect = 3.4
Identities = 15/56 (26%), Positives = 25/56 (43%)
Frame = +3
Query: 36 DINDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYS 91
+++ PH N AVT + +R+ + ++F N N + S S G YS
Sbjct: 45 NLHHPHRLHHLNLAVTNFTKRTSSTIRFASSTN--------QNQSSSSSPSPGLYS 188
>TC11370 similar to UP|Q8L539 (Q8L539) 5-formyltetrahydrofolate cycloligase
, partial (65%)
Length = 653
Score = 24.3 bits (51), Expect = 5.8
Identities = 11/17 (64%), Positives = 15/17 (87%)
Frame = -2
Query: 2 RNPTLLLVLLFVVVLFS 18
R+ LLL+LLFVVV+F+
Sbjct: 115 RSSWLLLLLLFVVVIFN 65
>BP048729
Length = 543
Score = 23.9 bits (50), Expect = 7.6
Identities = 12/38 (31%), Positives = 17/38 (44%)
Frame = -3
Query: 66 VINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIW 103
V N Q NY+L + +DG S + + KIW
Sbjct: 307 VPNLGKQKTRSNNYKLLSAVTDGHCSGSVAVSIGRKIW 194
>TC14607
Length = 582
Score = 23.9 bits (50), Expect = 7.6
Identities = 15/47 (31%), Positives = 29/47 (60%), Gaps = 3/47 (6%)
Frame = -1
Query: 70 ESQVVAGTNYRLTLSASDGSYSKNYE-AVVWEKIWQ--HFRNLTSFV 113
ES+++ +N+R+ L + + + K Y+ AV+WEK + H ++ T V
Sbjct: 417 ESKIMGASNHRM-LPSYNTHFHKIYKLAVLWEKAYMSLHSQHQTMIV 280
>TC17291 similar to UP|Q8LG11 (Q8LG11) Proline-rich cell wall protein-like,
partial (33%)
Length = 429
Score = 23.5 bits (49), Expect = 9.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = -3
Query: 19 YAAARNQFAPGGWSPI 34
Y R APGGW PI
Sbjct: 223 YDEGRPTAAPGGWGPI 176
>AV418977
Length = 415
Score = 23.5 bits (49), Expect = 9.9
Identities = 12/31 (38%), Positives = 15/31 (47%)
Frame = +2
Query: 70 ESQVVAGTNYRLTLSASDGSYSKNYEAVVWE 100
+ +VAGT RL L G YS +WE
Sbjct: 209 DGYLVAGTQKRLFLVGRIGLYSTLQSMRIWE 301
>TC10393
Length = 1063
Score = 23.5 bits (49), Expect = 9.9
Identities = 10/13 (76%), Positives = 12/13 (91%)
Frame = -3
Query: 5 TLLLVLLFVVVLF 17
TLLL LLFV+V+F
Sbjct: 146 TLLLYLLFVIVIF 108
>AV428920
Length = 439
Score = 23.5 bits (49), Expect = 9.9
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +1
Query: 69 GESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVPVH 116
GE + GT ++ SD ++ + ++ + IW+ F F P H
Sbjct: 271 GEEDIYHGTPIYISTWRSDTLWTW*WVFILHDGIWRRFX*SIQFCPKH 414
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,036,018
Number of Sequences: 28460
Number of extensions: 21426
Number of successful extensions: 108
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of query: 117
length of database: 4,897,600
effective HSP length: 78
effective length of query: 39
effective length of database: 2,677,720
effective search space: 104431080
effective search space used: 104431080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)
Medicago: description of AC148653.2