BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.1 - phase: 0
(693 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417841 72 3e-13
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 67 1e-11
AV427422 57 1e-08
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 55 3e-08
BF177840 47 1e-05
TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylas... 30 1.7
AV422889 29 2.2
AV409405 27 8.4
>BI417841
Length = 617
Score = 72.0 bits (175), Expect = 3e-13
Identities = 39/123 (31%), Positives = 68/123 (54%), Gaps = 2/123 (1%)
Frame = +1
Query: 121 LIFDGAV--NVYGSGIGAIIVTPEGAHIPFTARLTFDCTNNIAEYEACIMGIEEAIDLRI 178
L FDG+ N +G GA++ +G+ + + + TNN AEY I+G++ A +
Sbjct: 142 LEFDGSSKGNPGSAGAGAVLRAEDGSKV-YLREGVGNQTNNQAEYRGLILGLKHAHEQGY 318
Query: 179 KNLDIYGDSALVINQITGEWETRHPGLVPYKDYARRLLTFFNKVELHHIPRDENQMADAL 238
+++++ GDS LV Q+ G W+ R+P + + A+ L + F +++H+PR N AD
Sbjct: 319 QHINVKGDSQLVCKQVEGSWKARNPNIASLCNEAKELKSKFQSFDINHVPRQYNSEADVQ 498
Query: 239 ATL 241
A L
Sbjct: 499 ANL 507
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 66.6 bits (161), Expect = 1e-11
Identities = 36/96 (37%), Positives = 52/96 (53%), Gaps = 1/96 (1%)
Frame = +2
Query: 475 DNGTNLNNKMMKELCDDFKIEHHNSSPYRPQMNGAVEAANKNIKKIIQKMVVTYKD-WHE 533
+NG +K ++ CD I+ SS PQ NG EAANK I K I++ + + W +
Sbjct: 8 ENGIQFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWID 187
Query: 534 MLPFALHGYRTSIRTSTGATPFSLVYGMEAVLPIEV 569
LP L Y T ++S TPF L YG + +LP+E+
Sbjct: 188 ELPIVLWSYNTMPQSSIKETPF*LTYGADTMLPVEI 295
>AV427422
Length = 417
Score = 56.6 bits (135), Expect = 1e-08
Identities = 34/133 (25%), Positives = 61/133 (45%), Gaps = 2/133 (1%)
Frame = +1
Query: 424 SNGHRFILVAIDYFTKWVEAASYAN-VTKQVVVKFIKNHIICRYGVPSRIITDNGTNLNN 482
S G+ +LV +D +K+ + T +V+ ++ +GVP I++D +
Sbjct: 19 SKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDPLFMS 198
Query: 483 KMMKELCDDFKIEHHNSSPYRPQMNGAVEAANKNIKKIIQKMVVTY-KDWHEMLPFALHG 541
KEL + S+ Y P+ +G E N+ ++ ++ + K W +P+A +
Sbjct: 199 NFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPWAEYW 378
Query: 542 YRTSIRTSTGATP 554
Y TS STG TP
Sbjct: 379 YNTSYHVSTGQTP 417
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 55.5 bits (132), Expect = 3e-08
Identities = 28/91 (30%), Positives = 46/91 (49%)
Frame = +2
Query: 163 YEACIMGIEEAIDLRIKNLDIYGDSALVINQITGEWETRHPGLVPYKDYARRLLTFFNKV 222
Y I+G++ AI K++ + GDS LV NQ+ G W+ ++ + A+ L F
Sbjct: 2 YRGLILGLKHAIKEGYKHIQVKGDSMLVCNQVQGLWKIKNQNIASLCSEAKELKNKFLSF 181
Query: 223 ELHHIPRDENQMADALATLSSMYQVNRWNEV 253
+++HIPR+ N AD A + + EV
Sbjct: 182 KINHIPREYNSEADVQANFGISLRAGQVEEV 274
>BF177840
Length = 410
Score = 47.0 bits (110), Expect = 1e-05
Identities = 29/129 (22%), Positives = 63/129 (48%), Gaps = 3/129 (2%)
Frame = +2
Query: 472 IITDNGTNLNNKMMKELCDDFKIEHHNSSPYRPQMNGAVEAANKNIKKIIQKMVV-TYKD 530
I++D T + + L + S+ PQ +G E NK + +++ ++ K
Sbjct: 17 IVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNLKM 196
Query: 531 WHEMLPFALHGYRTSIRTSTGATPFSLVYGMEAVLPIE-VEIPSIRVLMEAE-LSEAEWV 588
W LP Y + ++T +PF +VYG + P++ + +P+ +L + ++AE+V
Sbjct: 197 WETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPNTYLLKHKDGKAKAEFV 376
Query: 589 QSRYDQLNL 597
+ ++++ L
Sbjct: 377 KRLHEKIKL 403
>TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylase, complete
Length = 1917
Score = 29.6 bits (65), Expect = 1.7
Identities = 28/123 (22%), Positives = 53/123 (42%), Gaps = 11/123 (8%)
Frame = +3
Query: 418 RIEPKASNGHRFILVAIDYFTKWVEAASYANVTKQVVVKFIKNHIICRYGVPSRIITDNG 477
R+ S ++ + A DYF+KW + +V +K+ H+I I+T +
Sbjct: 513 RVNKLKSYVNQMVAEAEDYFSKWGSSG-------EVDLKYELEHLI--------ILTASR 647
Query: 478 TNLNNKMMKELCDDFKIEHHNSS----------PYRP-QMNGAVEAANKNIKKIIQKMVV 526
+ L ++ +L DD H+ PY P + + A K + +I +K++V
Sbjct: 648 SLLGREVRDKLFDDVSALFHDLDNGMLPISVLFPYLPIPAHKRRDQARKKLSEIFEKIIV 827
Query: 527 TYK 529
+ K
Sbjct: 828 SRK 836
>AV422889
Length = 427
Score = 29.3 bits (64), Expect = 2.2
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 258 IRHLERPAHVFAIEEVVDDKPWFHDIKCFL 287
+R L R +H + + + +KPW H IKC L
Sbjct: 384 LRKLRRGSH*ISFSKCLPNKPWGHCIKCRL 295
>AV409405
Length = 424
Score = 27.3 bits (59), Expect = 8.4
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +2
Query: 378 HAKRCYKCQIYADRIHVPPTALNVLSSPW 406
H K+C C + +I V P LN L S W
Sbjct: 35 HCKKCCACHLIYRKISVLPGNLNNLPSNW 121
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,607,703
Number of Sequences: 28460
Number of extensions: 176907
Number of successful extensions: 731
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 724
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 729
length of query: 693
length of database: 4,897,600
effective HSP length: 97
effective length of query: 596
effective length of database: 2,136,980
effective search space: 1273640080
effective search space used: 1273640080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148653.1