BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.6 + phase: 0 /partial
(310 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8429 weakly similar to UP|BAD03306 (BAD03306) MAP3K-like prote... 475 e-135
AV770798 120 2e-28
AW719881 112 6e-26
BP063326 106 4e-24
BP053195 38 0.002
TC14497 27 3.4
TC10902 similar to UP|O04794 (O04794) Acyl-ACP thioesterase, par... 27 5.8
TC7847 homologue to UP|SUSY_SOYBN (P13708) Sucrose synthase (Su... 26 7.5
AV773264 26 7.5
BP034433 26 7.5
AV418055 26 9.8
TC12582 similar to UP|Q7XYX6 (Q7XYX6) AKIN beta3, partial (42%) 26 9.8
TC14038 homologue to UP|Q9LN13 (Q9LN13) T6D22.2, partial (47%) 26 9.8
>TC8429 weakly similar to UP|BAD03306 (BAD03306) MAP3K-like protein kinase,
partial (55%)
Length = 1298
Score = 475 bits (1223), Expect = e-135
Identities = 224/310 (72%), Positives = 258/310 (82%), Gaps = 1/310 (0%)
Frame = +3
Query: 1 QIGETCSRDVNDCGTGLQCLECNSQSRCTRVRTSSPISKVMELPFNHYSWLTTHNSYASR 60
QI ETCSRD+NDC GLQCLEC+SQ+RCTR+RT SP SKVMELPFN YSWLTTHNS+A++
Sbjct: 156 QIAETCSRDINDCDLGLQCLECHSQNRCTRIRTISPTSKVMELPFNEYSWLTTHNSFAAK 335
Query: 61 AANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWLCRGPCTIFTAFQPAIN 120
N S S + + NQEDSITDQL+NGVRG+MLDM DY IWLCRGPCT +T FQPA+N
Sbjct: 336 GVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYEDTIWLCRGPCTKYTTFQPALN 515
Query: 121 VLREINTFLTRHRTEIVTVFIKDRVTSPNGVNKVFNKAGLRKFWFPVYKMPKNGSDWLTV 180
VL+E+ FL H TEI+T+FI D VTS NGVNKVF+KA LRKFWFPV KMPKNGSDW TV
Sbjct: 516 VLKEVRVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKARLRKFWFPVSKMPKNGSDWPTV 695
Query: 181 KKMLRMNHRLIVFTSNATKEASERIAYEWNYVVENKYGNDGMGRDHCLHRAESYPMNTTT 240
K M+R N+RLIVFTSNA++EASE IAYEWNYVVE+++GN G+ C +R ES PMN T
Sbjct: 696 KTMIRKNYRLIVFTSNASREASEGIAYEWNYVVESQFGNVGIKGGSCQNRPESLPMNNAT 875
Query: 241 KSLVLMNYYRNVLN-SNEACKDNSSPLIRKMHTCYKDAGNRWPNYIAVDFYKRGDGGGAP 299
KSLVLMNY+RNV N +EAC+DNSSPLI MH C++ AGNRWPN+IAVDFYKRGDGGGAP
Sbjct: 876 KSLVLMNYFRNVRNHDDEACRDNSSPLIAMMHVCFRAAGNRWPNFIAVDFYKRGDGGGAP 1055
Query: 300 EALDVANRNL 309
EALD+ANRNL
Sbjct: 1056EALDLANRNL 1085
>AV770798
Length = 503
Score = 120 bits (302), Expect = 2e-28
Identities = 63/142 (44%), Positives = 89/142 (62%), Gaps = 7/142 (4%)
Frame = +1
Query: 1 QIGETCSRDVNDCGTGLQCLECN---SQSRCTRVRTSSPISKVME-LPFNHYSWLTTHNS 56
Q+ E CS D +DCG+GL C C S+SRC R + + LPFN Y++LTTHN+
Sbjct: 79 QLLEECSSD-DDCGSGLYCFSCGAKFSRSRCVRSSITDQFKLINNSLPFNKYAFLTTHNA 255
Query: 57 YASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWLC---RGPCTIFT 113
+A +++NQEDS+T QL+NG RG+MLD +DY GD+WLC +G C +T
Sbjct: 256 FAMDGEPSHTGVPRLTIINQEDSVTQQLKNGGRGLMLDTYDYDGDVWLCHSFQGKCHDYT 435
Query: 114 AFQPAINVLREINTFLTRHRTE 135
AF+PAI+ L+E+ FL+ + E
Sbjct: 436 AFEPAIDTLKEVAAFLSANPKE 501
>AW719881
Length = 495
Score = 112 bits (281), Expect = 6e-26
Identities = 54/108 (50%), Positives = 72/108 (66%), Gaps = 4/108 (3%)
Frame = +1
Query: 1 QIGETCSRDVNDCGTGLQCLEC----NSQSRCTRVRTSSPISKVMELPFNHYSWLTTHNS 56
+IGETC C GL C C N++ RC+R++ S+P +KV LPFN YSWLT+HNS
Sbjct: 184 KIGETCG----SCDGGLTCQTCPANGNTRPRCSRIQPSNPTTKVKGLPFNRYSWLTSHNS 351
Query: 57 YASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWL 104
+A A + S I + MNQ+D++ QL+NGVRG MLDM+D+ DIWL
Sbjct: 352 FAQAGARSATGSFIIAPMNQDDTVAQQLKNGVRGFMLDMYDFQNDIWL 495
>BP063326
Length = 492
Score = 106 bits (265), Expect = 4e-24
Identities = 50/75 (66%), Positives = 58/75 (76%), Gaps = 1/75 (1%)
Frame = -3
Query: 218 GNDGMGRDHCLHRAESYPMNTTTKSLVLMNYYRNVLN-SNEACKDNSSPLIRKMHTCYKD 276
GN G+ C +R ES PMN TKSLVLMNY+RNV N +EAC+DNSSPLI MH C++
Sbjct: 394 GNVGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNHDDEACRDNSSPLIAMMHVCFRA 215
Query: 277 AGNRWPNYIAVDFYK 291
AGNRWPN+IAVDFYK
Sbjct: 214 AGNRWPNFIAVDFYK 170
>BP053195
Length = 417
Score = 38.1 bits (87), Expect = 0.002
Identities = 16/23 (69%), Positives = 20/23 (86%)
Frame = -2
Query: 287 VDFYKRGDGGGAPEALDVANRNL 309
VD+Y R DGGGAPEA+DVA+ +L
Sbjct: 416 VDYYPRSDGGGAPEAVDVASGHL 348
>TC14497
Length = 874
Score = 27.3 bits (59), Expect = 3.4
Identities = 22/85 (25%), Positives = 38/85 (43%), Gaps = 4/85 (4%)
Frame = +3
Query: 42 ELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDM----HD 97
ELP +H+ N A++AA +S+ S D+ +++ +N + H+
Sbjct: 522 ELPKSHF------NPLAAKAATISLSSFGFGGPFSFDAFSEKWKNQQKKSKSSKDGSKHE 683
Query: 98 YYGDIWLCRGPCTIFTAFQPAINVL 122
+ WL G C I +F+ NVL
Sbjct: 684 AASNEWLQNGNCPIAKSFRAVSNVL 758
>TC10902 similar to UP|O04794 (O04794) Acyl-ACP thioesterase, partial (11%)
Length = 810
Score = 26.6 bits (57), Expect = 5.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -1
Query: 160 LRKFWFPVYKMPKNGSDWLTVKKMLRMN 187
+R+FW P+ P N S + K+ML +N
Sbjct: 123 VRRFWPPLSSAPHNFSAFFKSKQMLALN 40
>TC7847 homologue to UP|SUSY_SOYBN (P13708) Sucrose synthase (Sucrose-UDP
glucosyltransferase) (Nodulin-100) , complete
Length = 2797
Score = 26.2 bits (56), Expect = 7.5
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 122 LREINTFLTRHRTEIVTVFIKDRVTSPNGVNKVFNKA 158
L + FL H T+ + DR+ +PN + V KA
Sbjct: 604 LHPLLEFLRLHNYNGKTLMLNDRIQTPNALQHVLRKA 714
>AV773264
Length = 438
Score = 26.2 bits (56), Expect = 7.5
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = -3
Query: 50 WLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLR 85
WLT H+S A+L ++S ++ QE I +R
Sbjct: 391 WLTHHSSCPFSRASLKVESLFPKILIQEPPIRIDIR 284
>BP034433
Length = 490
Score = 26.2 bits (56), Expect = 7.5
Identities = 23/105 (21%), Positives = 39/105 (36%)
Frame = +2
Query: 17 LQCLECNSQSRCTRVRTSSPISKVMELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQ 76
L+C C S S C+ + I + YS T H + S S MN
Sbjct: 173 LRC*PCPSWSSCSS*KLYLDIYRRAPKTCTDYSCWTCHGARTYSCFIWSSTSST*ESMNS 352
Query: 77 EDSITDQLRNGVRGIMLDMHDYYGDIWLCRGPCTIFTAFQPAINV 121
+ + + GV + + G++W IFT+ P+ ++
Sbjct: 353 YGNKMSKCQFGVSFMQCAVLASCGNVWCRNFR*NIFTSLSPSFHI 487
>AV418055
Length = 407
Score = 25.8 bits (55), Expect = 9.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 12 DCGTGLQCLECNSQSRCTRVRTSSP 36
D G G QCL C++ R T T+SP
Sbjct: 71 DAGIGAQCLSCSALQRLT---TASP 6
>TC12582 similar to UP|Q7XYX6 (Q7XYX6) AKIN beta3, partial (42%)
Length = 815
Score = 25.8 bits (55), Expect = 9.8
Identities = 11/31 (35%), Positives = 18/31 (57%), Gaps = 3/31 (9%)
Frame = -3
Query: 209 WNYVVE---NKYGNDGMGRDHCLHRAESYPM 236
W+ +VE NK+ + MG +C HR+ P+
Sbjct: 639 WDGLVEHDCNKF*TEAMGESYCHHRSGGLPI 547
>TC14038 homologue to UP|Q9LN13 (Q9LN13) T6D22.2, partial (47%)
Length = 1844
Score = 25.8 bits (55), Expect = 9.8
Identities = 8/12 (66%), Positives = 9/12 (74%)
Frame = -3
Query: 216 KYGNDGMGRDHC 227
KYGN G+G HC
Sbjct: 1149 KYGNQGLGHSHC 1114
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,957,382
Number of Sequences: 28460
Number of extensions: 80620
Number of successful extensions: 411
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 405
length of query: 310
length of database: 4,897,600
effective HSP length: 90
effective length of query: 220
effective length of database: 2,336,200
effective search space: 513964000
effective search space used: 513964000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148528.6