BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148486.4 - phase: 0
(340 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding prot... 280 2e-76
AV420147 134 3e-32
BP051594 80 2e-30
TC18699 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromo... 75 2e-14
TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12... 66 7e-12
TC13326 64 5e-11
TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 62 2e-10
BP077155 59 2e-09
BP030839 57 4e-09
TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 52 2e-07
TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-relate... 51 2e-07
TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (... 51 3e-07
BP059128 48 2e-06
AV779203 45 2e-05
AV766525 44 3e-05
BP071727 44 4e-05
BP043656 42 2e-04
TC14881 41 3e-04
TC17849 40 4e-04
TC15471 38 0.002
>TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding protein SBP1,
partial (48%)
Length = 611
Score = 280 bits (717), Expect = 2e-76
Identities = 132/149 (88%), Positives = 143/149 (95%)
Frame = +2
Query: 192 VLQKLREKETEVENINKRNMELEDQMEQLSVEAGAWQQRARYNENMIAALKFNLQQAYLQ 251
VLQ LR++E EVENINKRNMELED+MEQL+VEAGAWQQRARYNENMIAALK+NLQQAY+Q
Sbjct: 2 VLQILRDQEAEVENINKRNMELEDRMEQLAVEAGAWQQRARYNENMIAALKYNLQQAYVQ 181
Query: 252 GRDSKEGCGDSEVDDTASCCNGRSLDFHLLSNENSNMKDLMKCKACRVNEVTMVLLPCKH 311
RDSKEGCGDSEVDDTASCCNGR+LDFHLLS NS+MK++M CKACRVNEVTMVLLPCKH
Sbjct: 182 SRDSKEGCGDSEVDDTASCCNGRTLDFHLLSKGNSDMKEMMTCKACRVNEVTMVLLPCKH 361
Query: 312 LCLCKDCESKLSFCPLCQSSKFIGMEVYM 340
LCLCKDCESKLSFCPLCQSSKFIGMEV+M
Sbjct: 362 LCLCKDCESKLSFCPLCQSSKFIGMEVFM 448
>AV420147
Length = 274
Score = 134 bits (336), Expect = 3e-32
Identities = 65/86 (75%), Positives = 72/86 (83%), Gaps = 4/86 (4%)
Frame = +1
Query: 1 MAFLQDQFQRHYQQQQQPQPQTKSFRNLQTIEGQMSQQ--MAFYNPTDLQDQSQH--PPY 56
MAF QDQFQRHYQ QQ PQTK FR+L TI+GQM QQ +AFYNP+DLQDQ+ H PPY
Sbjct: 13 MAFPQDQFQRHYQTQQHHHPQTKPFRDLLTIDGQMQQQQQIAFYNPSDLQDQTHHHHPPY 192
Query: 57 IPPFHVVGFAPGPVIPADGSDGGVDL 82
PPFHVVGFAPGPV+PADGSDGG+DL
Sbjct: 193NPPFHVVGFAPGPVLPADGSDGGLDL 270
>BP051594
Length = 376
Score = 80.1 bits (196), Expect(2) = 2e-30
Identities = 34/38 (89%), Positives = 37/38 (96%)
Frame = -3
Query: 300 NEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGME 337
+EVT+VLLPCKHLCLCKDCESKLSFCPLC SSKFIG+E
Sbjct: 245 HEVTLVLLPCKHLCLCKDCESKLSFCPLCPSSKFIGLE 132
Score = 68.6 bits (166), Expect(2) = 2e-30
Identities = 29/41 (70%), Positives = 35/41 (84%)
Frame = -1
Query: 257 EGCGDSEVDDTASCCNGRSLDFHLLSNENSNMKDLMKCKAC 297
EGCGD EVDDTASCC+G +LDFHLLS NS++K++M KAC
Sbjct: 376 EGCGDREVDDTASCCHGPTLDFHLLSQGNSDLKEMMTSKAC 254
>TC18699 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromosome 5, TAC
clone:K17O22 (AT5g45100/K17O22_9), partial (28%)
Length = 537
Score = 75.1 bits (183), Expect = 2e-14
Identities = 42/137 (30%), Positives = 67/137 (48%), Gaps = 1/137 (0%)
Frame = +3
Query: 196 LREKETEVENINKRNMELEDQMEQLSVEAGAWQQRARYNENMIAALKFNLQQAYLQGRDS 255
++EK+ E++ + K N L +++ L E W++ A+ +E + +L+ +L+Q DS
Sbjct: 3 MKEKDDEIQKLAKVNFVLHERVNSLCEENQIWRELAQAHEAAVISLRSDLEQVLAHVSDS 182
Query: 256 KEGCGDSEVDDTASCCNGRSLDFHLLSNENSNMKDLMKCKACRVNEVTMVLLPCKHLCLC 315
G E DD S C + H E N C+ C E ++LLPC+HLCL
Sbjct: 183 HRGAAAEEEDDAESSCGS---NHHREEEEEENEAVGNLCRECGAQESGVLLLPCRHLCL* 353
Query: 316 KDC-ESKLSFCPLCQSS 331
D + CPLC S+
Sbjct: 354 YDVWVQPIRNCPLCHSA 404
>TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12920
{Arabidopsis thaliana;}, partial (27%)
Length = 730
Score = 66.2 bits (160), Expect = 7e-12
Identities = 33/108 (30%), Positives = 62/108 (56%)
Frame = +1
Query: 141 SLIGDDIDRELQQQDLEMDRFLKLQGEQLRQTILEKVQATQLQSVSIIEDKVLQKLREKE 200
S +G+DI + L++D + E++R + EK + + + IE ++++L+ KE
Sbjct: 400 SFLGEDISLHIHHHQLDIDNLISQHMEKVRMELEEKRKGQVRRLMEAIEIGMMKRLKTKE 579
Query: 201 TEVENINKRNMELEDQMEQLSVEAGAWQQRARYNENMIAALKFNLQQA 248
E+E I K N+ LE++++ L +E W++ A+ NE AL+ NL+ A
Sbjct: 580 DEIERIGKLNLALEERVKSLYIENQIWRELAQTNEATANALRANLEHA 723
>TC13326
Length = 578
Score = 63.5 bits (153), Expect = 5e-11
Identities = 38/139 (27%), Positives = 61/139 (43%), Gaps = 9/139 (6%)
Frame = +2
Query: 208 KRNMELEDQMEQLSVEAGAWQQRARYNENMIAALKFNLQQAYLQGRDSKEGCGDSEVDDT 267
K+ ELE+ + +L E W++ A+ NE ++ +L L+Q KE +D
Sbjct: 8 KKKTELEEFLIRLEAENQLWRRVAQENEAVVLSLHNTLEQV-------KERNRGLVAEDA 166
Query: 268 ASCCNGRSLDFHLLSNENSNMKDL---------MKCKACRVNEVTMVLLPCKHLCLCKDC 318
S C + ++ +++ M CK C + LPC+HLC CK C
Sbjct: 167 ESYCCDENRGNRVMQEGTGETEEIGKETTEQITMVCKCCHSRSSCFMFLPCRHLCSCKAC 346
Query: 319 ESKLSFCPLCQSSKFIGME 337
E L CP+C+ K +E
Sbjct: 347 EPFLQACPVCKMPKKTSIE 403
>TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (16%)
Length = 487
Score = 61.6 bits (148), Expect = 2e-10
Identities = 32/84 (38%), Positives = 45/84 (53%), Gaps = 7/84 (8%)
Frame = +2
Query: 264 VDDTASCCNGRSLD---FHLLSNENSNMKDLMK----CKACRVNEVTMVLLPCKHLCLCK 316
VDD SCC D + L+ E + +KD C+ C +E +++LPC+HLCLC
Sbjct: 44 VDDAESCCGSTDGDDEGWRTLAGECAGVKDKDSGSRLCRNCGKDESCVLILPCRHLCLCT 223
Query: 317 DCESKLSFCPLCQSSKFIGMEVYM 340
C S L CP+C+S K + V M
Sbjct: 224 VCGSTLHTCPICKSFKTASVHVNM 295
>BP077155
Length = 432
Score = 58.5 bits (140), Expect = 2e-09
Identities = 33/113 (29%), Positives = 59/113 (52%)
Frame = -2
Query: 228 QQRARYNENMIAALKFNLQQAYLQGRDSKEGCGDSEVDDTASCCNGRSLDFHLLSNENSN 287
Q +A+ E A+L+ LQ+A + G D G SE ++A R ++
Sbjct: 431 QAKAKEQEATAASLQAQLQRAIMSGGDG----GLSEDAESAYVDPERVVE---------- 294
Query: 288 MKDLMKCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGMEVYM 340
+ +CK C ++V+LPC+HLC+C +C+++ CP+C + K ++VY+
Sbjct: 293 VGPTRECKGCWKRVASVVVLPCRHLCVCTECDAQFRACPVCLTLKNSSVQVYL 135
>BP030839
Length = 379
Score = 57.0 bits (136), Expect = 4e-09
Identities = 38/121 (31%), Positives = 55/121 (45%), Gaps = 20/121 (16%)
Frame = -3
Query: 240 ALKFNLQQAYLQGRDSKEGCGDSEV---------DDTASCCNGRSLDFHLLSNENSNMKD 290
AL+ NL+ A Q RDS + D+ V DD SCC + D + ++D
Sbjct: 377 ALRANLEHALCQIRDSDDE--DATVVPPAMAAPEDDAESCCGSNNDDDGWRTVVQLGVQD 204
Query: 291 L-----------MKCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGMEVY 339
C+ C E +++LPC+HLC+C C S L CP+C+S K + V
Sbjct: 203 KECGGPESDRRRRLCRKCGKEESCVLILPCRHLCVCTLCGSTLHHCPICKSYKNASVHVN 24
Query: 340 M 340
M
Sbjct: 23 M 21
>TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (14%)
Length = 547
Score = 51.6 bits (122), Expect = 2e-07
Identities = 19/37 (51%), Positives = 26/37 (69%)
Frame = +3
Query: 294 CKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQS 330
C++C V E ++LLPC+HLCLC C S + CP+C S
Sbjct: 78 CRSCGVKESVVLLLPCRHLCLCTACGSTVRNCPVCDS 188
>TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-related protein
{Arabidopsis thaliana;} , partial (8%)
Length = 735
Score = 51.2 bits (121), Expect = 2e-07
Identities = 18/38 (47%), Positives = 24/38 (62%)
Frame = +1
Query: 294 CKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSS 331
CK C + +LLPC+H CLCK C S CP+C++S
Sbjct: 253 CKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRTS 366
>TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (17%)
Length = 489
Score = 50.8 bits (120), Expect = 3e-07
Identities = 17/48 (35%), Positives = 31/48 (64%)
Frame = +3
Query: 293 KCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGMEVYM 340
KC+ C ++V+LPC+H+C+C +C++ CP+C + K +EV +
Sbjct: 57 KCRGCERRVASVVVLPCRHMCICAECDAHFRACPVCLTLKNSTVEVLL 200
>BP059128
Length = 362
Score = 48.1 bits (113), Expect = 2e-06
Identities = 18/48 (37%), Positives = 28/48 (57%)
Frame = -3
Query: 290 DLMKCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGME 337
++M CK+C + + LPC+HL CK C++ L CP+C K +E
Sbjct: 348 EVMVCKSCHSRKSCFLFLPCRHLASCKSCDAILKACPVCGMQKKSSIE 205
>AV779203
Length = 487
Score = 44.7 bits (104), Expect = 2e-05
Identities = 18/46 (39%), Positives = 26/46 (56%)
Frame = -1
Query: 294 CKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGMEVY 339
C+ C E+++VLLPC+H LC C K CP+ + S + VY
Sbjct: 379 CRICFEGEISVVLLPCRHRVLCSTCSEKCKKCPISRDSISERVPVY 242
>AV766525
Length = 564
Score = 44.3 bits (103), Expect = 3e-05
Identities = 16/36 (44%), Positives = 20/36 (55%)
Frame = -1
Query: 294 CKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQ 329
C C E V +PC H+C C C S L+ CPLC+
Sbjct: 414 CVICLEKEYNSVFVPCGHMCCCTGCSSHLTSCPLCR 307
>BP071727
Length = 391
Score = 43.9 bits (102), Expect = 4e-05
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = -1
Query: 295 KACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQS 330
+ C E +++LPC+HLC+C C S L CP+C+S
Sbjct: 178 RKCGKEESCVLILPCRHLCVCTLCGSTLHNCPICKS 71
>BP043656
Length = 412
Score = 41.6 bits (96), Expect = 2e-04
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = -2
Query: 295 KACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQ 329
K C + +LLP +H CLCK C S CP+C+
Sbjct: 411 KVCFESPTAAILLPSRHFCLCKSCSLACSECPICR 307
>TC14881
Length = 609
Score = 40.8 bits (94), Expect = 3e-04
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Frame = +3
Query: 293 KCKACRVNEVTMVLLPCKHLCLCKDCESKLSF----CPLCQ 329
+C C +LPC+H+C+C DC +L CP+C+
Sbjct: 96 ECVICMTEPKDTAVLPCRHMCMCSDCAKELRLQSNKCPICR 218
>TC17849
Length = 589
Score = 40.4 bits (93), Expect = 4e-04
Identities = 17/48 (35%), Positives = 26/48 (53%)
Frame = +2
Query: 282 SNENSNMKDLMKCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQ 329
+ E K C+ C EV + ++PC H+ LC+ C S +S CP C+
Sbjct: 131 TKEADTAKAAWVCRVCLSAEVDITIVPCGHV-LCRKCSSAVSKCPFCR 271
>TC15471
Length = 642
Score = 38.1 bits (87), Expect = 0.002
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 4/61 (6%)
Frame = +3
Query: 273 GRSLDFHLLSNENSNMKDLMKCKACRVNEVTMVLLPCKHLCLCKDCESKLSF----CPLC 328
G S++ L N+ +C C +LPC+H+C+C C L F CP+C
Sbjct: 126 GNSMESDLDGNDPGK-----ECVICLSEPRDTTVLPCRHMCMCSGCAKVLRFQTNRCPIC 290
Query: 329 Q 329
+
Sbjct: 291 R 293
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,401,740
Number of Sequences: 28460
Number of extensions: 76109
Number of successful extensions: 607
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 597
length of query: 340
length of database: 4,897,600
effective HSP length: 91
effective length of query: 249
effective length of database: 2,307,740
effective search space: 574627260
effective search space used: 574627260
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148486.4