BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148485.5 + phase: 0
(102 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP046665 127 2e-31
TC8084 similar to UP|PIF1_YEAST (P07271) DNA repair and recombin... 117 2e-28
TC15490 weakly similar to UP|Q9LTU4 (Q9LTU4) Helicase-like prote... 89 5e-27
TC8086 similar to UP|DMC1_HUMAN (Q14565) Meiotic recombination p... 82 2e-17
AU089042 62 1e-11
BP065488 54 5e-09
TC16561 similar to PIR|E86431|E86431 T5I8.7 protein - Arabidopsi... 27 0.42
BP050969 27 0.72
TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-lik... 27 0.72
BP042340 25 2.1
BP042870 25 2.7
TC14118 homologue to UP|AAS18240 (AAS18240) Enolase , complete 25 2.7
TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate... 25 2.7
TC14280 weakly similar to UP|Q94IC5 (Q94IC5) BURP domain-contain... 24 4.7
AV776118 23 6.1
TC13549 similar to UP|Q9STG6 (Q9STG6) dUTP pyrophosphatase-like ... 23 8.0
>BP046665
Length = 524
Score = 127 bits (320), Expect = 2e-31
Identities = 64/102 (62%), Positives = 77/102 (74%)
Frame = -1
Query: 1 MLLRNVDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPSGANVSITF 60
MLLRN+ QA G CNGTRLI+ LG NVI A VI + G+ ++IPRM+++PS + F
Sbjct: 464 MLLRNIYQAVGFCNGTRLIVADLGTNVIKATVIT*TNIGDDIFIPRMDMVPSDSGYPFKF 285
Query: 61 ERRQFPLVLSFAMTINKSQGQTLTSVGLYLPRPVFTHGQLYV 102
ERRQFP+ L AMTINKSQGQ+L+ VGLYL R VFTHGQLYV
Sbjct: 284 ERRQFPISLCSAMTINKSQGQSLSHVGLYLSRHVFTHGQLYV 159
>TC8084 similar to UP|PIF1_YEAST (P07271) DNA repair and recombination
protein PIF1, mitochondrial precursor, partial (4%)
Length = 560
Score = 117 bits (294), Expect = 2e-28
Identities = 57/97 (58%), Positives = 72/97 (73%)
Frame = +1
Query: 6 VDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPSGANVSITFERRQF 65
+ + LCNGTRLI+V LG VI A VI G + G+ ++IPR++++PS + FERR F
Sbjct: 43 IQKFENLCNGTRLIVVDLGTYVIKATVITGTNIGDDIFIPRLDMVPSDSGYPFKFERR*F 222
Query: 66 PLVLSFAMTINKSQGQTLTSVGLYLPRPVFTHGQLYV 102
P+ L FAMTINKSQGQ+L+ V LYL RPVFTHGQLYV
Sbjct: 223 PISLCFAMTINKSQGQSLSHVSLYLSRPVFTHGQLYV 333
>TC15490 weakly similar to UP|Q9LTU4 (Q9LTU4) Helicase-like protein, partial
(8%)
Length = 634
Score = 89.0 bits (219), Expect(2) = 5e-27
Identities = 43/71 (60%), Positives = 54/71 (75%)
Frame = +2
Query: 32 VIGGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVGLYLP 91
VI G H G+ + IPRM+++PS ++ FERRQ P+ L FAMTINKSQG++L+ VGLYL
Sbjct: 149 VITGTHIGDDISIPRMDMVPSDSSYPFKFERRQSPISLCFAMTINKSQGRSLSHVGLYLS 328
Query: 92 RPVFTHGQLYV 102
RPV THG LYV
Sbjct: 329 RPVSTHG*LYV 361
Score = 45.4 bits (106), Expect(2) = 5e-27
Identities = 21/26 (80%), Positives = 23/26 (87%)
Frame = +1
Query: 1 MLLRNVDQASGLCNGTRLIIVSLGKN 26
MLLRN+ QASGLCNGTRLI+V LG N
Sbjct: 61 MLLRNIVQASGLCNGTRLIVVDLGIN 138
>TC8086 similar to UP|DMC1_HUMAN (Q14565) Meiotic recombination protein
DMC1/LIM15 homolog, partial (12%)
Length = 628
Score = 81.6 bits (200), Expect = 2e-17
Identities = 39/75 (52%), Positives = 53/75 (70%)
Frame = +1
Query: 6 VDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPSGANVSITFERRQF 65
+ + LC+GTRLI+V LG VI A VI G + G+ ++IPR++++PS + FERR F
Sbjct: 151 IQKFENLCHGTRLIVVDLGTYVIKATVITGTNIGDDIFIPRLDMVPSDSGYPFKFERR*F 330
Query: 66 PLVLSFAMTINKSQG 80
P+ L FAMTINKSQG
Sbjct: 331 PISLCFAMTINKSQG 375
>AU089042
Length = 191
Score = 62.4 bits (150), Expect = 1e-11
Identities = 30/52 (57%), Positives = 37/52 (70%)
Frame = +3
Query: 1 MLLRNVDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPS 52
ML+ N+ ++GLCNGTRLI+ LG NVI A ++ G H G VVYI MNL PS
Sbjct: 30 MLMXNLXISTGLCNGTRLIVDYLGPNVIGATILSGTHIGXVVYISMMNLXPS 185
>BP065488
Length = 439
Score = 53.5 bits (127), Expect = 5e-09
Identities = 24/41 (58%), Positives = 32/41 (77%)
Frame = -1
Query: 39 GEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQ 79
G+ ++IPRMN++PS + + FER QFP+ L FAMTINKSQ
Sbjct: 379 GDDIFIPRMNMVPSVSGDPLKFERCQFPISLCFAMTINKSQ 257
>TC16561 similar to PIR|E86431|E86431 T5I8.7 protein - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (20%)
Length = 511
Score = 27.3 bits (59), Expect = 0.42
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 3/41 (7%)
Frame = -1
Query: 56 VSITFERRQFPLVLSFAMTINKSQG---QTLTSVGLYLPRP 93
V +T+ RR F LVL + T+N S +T G +LP P
Sbjct: 343 VQLTWIRRGFLLVLGYVRTLNHSNYGI*NPITITG*FLPSP 221
>BP050969
Length = 557
Score = 26.6 bits (57), Expect = 0.72
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -1
Query: 10 SGLCNGTRLIIVSLGKNVICARV 32
S CN R++I+SLG +++ V
Sbjct: 377 SSYCNSCRIVIISLGMDIVMVNV 309
>TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-like protein,
partial (21%)
Length = 1193
Score = 26.6 bits (57), Expect = 0.72
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +1
Query: 68 VLSFAMTINKSQGQTLTSVGLYLPRPVFTH 97
VLS +TINKS+G T +YLPR H
Sbjct: 232 VLSKDLTINKSKG---TWARIYLPRKALDH 312
>BP042340
Length = 453
Score = 25.0 bits (53), Expect = 2.1
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 37 HAGEVVYIPRMNLIPSGANV 56
HA E+ PRMN P+ ANV
Sbjct: 190 HAAELRRSPRMNSAPAAANV 249
>BP042870
Length = 503
Score = 24.6 bits (52), Expect = 2.7
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = -1
Query: 73 MTINKSQGQTLTSVGLYLPRPVFTHGQLY 101
M I + + +T S LYLP P +HG Y
Sbjct: 329 MNI*RKRPRTDMSFNLYLPLPEKSHGTYY 243
>TC14118 homologue to UP|AAS18240 (AAS18240) Enolase , complete
Length = 1696
Score = 24.6 bits (52), Expect = 2.7
Identities = 14/54 (25%), Positives = 23/54 (41%)
Frame = +2
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P GA+ + + I K GQ +VG
Sbjct: 557 GGSHAGNKLAMQEFMVLPVGASSFKEAMKMGVEVYHHLKAVIKKKYGQDAVNVG 718
>TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate transporter
3.5, partial (39%)
Length = 1616
Score = 24.6 bits (52), Expect = 2.7
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = -1
Query: 37 HAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKS 78
H G+ V++P P A +S ER+ F LS + +N++
Sbjct: 1532 HQGKYVFVPIGLHSPKPAFISTIPERQTFIKALSISSLLNRN 1407
>TC14280 weakly similar to UP|Q94IC5 (Q94IC5) BURP domain-containing protein
(Fragment), partial (54%)
Length = 723
Score = 23.9 bits (50), Expect = 4.7
Identities = 13/40 (32%), Positives = 18/40 (44%)
Frame = +2
Query: 5 NVDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYI 44
NVD G T++ + G NV + GG H G V +
Sbjct: 212 NVDAGKGKPGHTKVDVGKGGVNVNAGKGSGGGHGGTNVNV 331
>AV776118
Length = 334
Score = 23.5 bits (49), Expect = 6.1
Identities = 9/24 (37%), Positives = 18/24 (74%)
Frame = +3
Query: 63 RQFPLVLSFAMTINKSQGQTLTSV 86
R+ P+VLSF+ + + S+G+ T++
Sbjct: 171 RRAPIVLSFSSSYSSSKGRVDTAI 242
>TC13549 similar to UP|Q9STG6 (Q9STG6) dUTP pyrophosphatase-like protein ,
partial (76%)
Length = 597
Score = 23.1 bits (48), Expect = 8.0
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -3
Query: 50 IPSGANVSITFERRQFPLVLSFAMT 74
+PSG + I+ R FPL +F T
Sbjct: 127 VPSGIEMLISVATRAFPLAGTFVST 53
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.141 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,622,007
Number of Sequences: 28460
Number of extensions: 18252
Number of successful extensions: 85
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 85
length of query: 102
length of database: 4,897,600
effective HSP length: 78
effective length of query: 24
effective length of database: 2,677,720
effective search space: 64265280
effective search space used: 64265280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 47 (22.7 bits)
Medicago: description of AC148485.5