BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148485.4 - phase: 0
(449 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066241 202 1e-52
AU252242 70 7e-13
AV417253 49 2e-06
TC9032 similar to GB|AAP21369.1|30102902|BT006561 At4g19200 {Ara... 42 3e-04
TC15252 similar to UP|Q7PSL3 (Q7PSL3) ENSANGP00000019748 (Fragme... 37 0.009
TC16883 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase... 36 0.011
TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-... 35 0.033
BP056337 35 0.033
TC15425 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall... 34 0.043
TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%) 34 0.043
AV411518 33 0.073
TC9920 similar to UP|Q9FF85 (Q9FF85) Emb|CAB80929.1, partial (34%) 33 0.096
TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase is... 33 0.096
TC19296 similar to UP|O22855 (O22855) At2g43410 protein, partial... 33 0.096
TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinog... 33 0.13
TC7794 homologue to UP|Q9M600 (Q9M600) Chlorophyll a/b binding p... 33 0.13
TC15554 similar to GB|AAO24533.1|27808506|BT003101 At2g41420 {Ar... 32 0.21
TC12394 weakly similar to UP|Q9FEV7 (Q9FEV7) Microtubule-associa... 32 0.21
TC14775 PIR|S14181|S14181 DNA-directed RNA polymerase largest c... 32 0.28
TC14213 32 0.28
>BP066241
Length = 531
Score = 202 bits (513), Expect = 1e-52
Identities = 97/135 (71%), Positives = 115/135 (84%)
Frame = +1
Query: 1 MSSSKQQQQFRCTQIPSKVLHFRNLPWECSDEELADLCSPFGKVMSTKCNVGVNQNQAFV 60
M+S Q QFR TQ PSKVLH RNLPWEC++EEL +L PFGKV++TKCNVG N+NQAF+
Sbjct: 82 MASVSTQPQFRYTQPPSKVLHLRNLPWECTEEELIELGKPFGKVVNTKCNVGANRNQAFI 261
Query: 61 QFAELNQAISMVSYYASSSEPAQVRGKTVYIQYSNRHHIVNSNSPGDIPGNVLLVTIEGV 120
+FA+LNQAI+M+SYYASSSEPAQVRGKTVY+QYSNR IVN+ + D+PGNVLLVTIEG
Sbjct: 262 EFADLNQAIAMISYYASSSEPAQVRGKTVYLQYSNRQEIVNNKTAADVPGNVLLVTIEGT 441
Query: 121 EAGDVSIDVIHLVFS 135
+A VSIDV+HLV S
Sbjct: 442 DARLVSIDVLHLVSS 486
>AU252242
Length = 416
Score = 70.1 bits (170), Expect = 7e-13
Identities = 41/112 (36%), Positives = 65/112 (57%), Gaps = 3/112 (2%)
Frame = +3
Query: 9 QFRCTQIPSKVLHFRNLPWECSDEELADLCSPFGKVMSTKCNVGVNQNQAFVQFAELNQA 68
QF T+ PSKV+H RN+ E S+ +L L PFG + TK + +NQA +Q +++ A
Sbjct: 75 QFAMTE-PSKVIHVRNVGHEISENDLLQLFQPFGVI--TKLVMLRAKNQALLQMDDVSSA 245
Query: 69 ISMVSYYASSSEPAQVRGKTVYIQYSNRHHIV---NSNSPGDIPGNVLLVTI 117
++ + +YA+ +RG+ VY+Q+S+ + S GD P +LLVTI
Sbjct: 246 VTAIQFYANMQ--PSIRGRNVYVQFSSHQELTTVDQSQGRGDEPNRILLVTI 395
>AV417253
Length = 323
Score = 48.5 bits (114), Expect = 2e-06
Identities = 40/120 (33%), Positives = 52/120 (43%), Gaps = 11/120 (9%)
Frame = +3
Query: 315 KLHLTYSRHTDLNVKAFSDKSRDYTVLDPSLHAAQAPAWQTTQAATMYSGSMGQMPS--W 372
KLH++YSRHTDL++K +D+SRDYT+ TT +GQ P
Sbjct: 6 KLHISYSRHTDLSIKVNNDRSRDYTI-------------PTTPVVNTQPSILGQQPGPLV 146
Query: 373 DPNQQEVTQSY--LSAPGTFPSGQA---AP----PFPGYSPAAVPPAGASPHSHMPPSSF 423
P QQ Y +S+ P QA AP P ++ VPP G P P F
Sbjct: 147 GPAQQYNGSQYTPISSQSLTPQSQAGWGAPAQSMPMQMHNNMYVPP-GTMPPQMAPAMQF 323
Score = 42.0 bits (97), Expect = 2e-04
Identities = 22/48 (45%), Positives = 28/48 (57%), Gaps = 3/48 (6%)
Frame = +3
Query: 190 LRISYSAHRDLNIKFQSNRSRDYTNPMLPV---NQAAIDSALQPAIGP 234
L ISYS H DL+IK ++RSRDYT P PV + + P +GP
Sbjct: 9 LHISYSRHTDLSIKVNNDRSRDYTIPTTPVVNTQPSILGQQPGPLVGP 152
>TC9032 similar to GB|AAP21369.1|30102902|BT006561 At4g19200 {Arabidopsis
thaliana;}, partial (50%)
Length = 502
Score = 41.6 bits (96), Expect = 3e-04
Identities = 23/64 (35%), Positives = 27/64 (41%), Gaps = 1/64 (1%)
Frame = +2
Query: 367 GQMPSWDPNQQEVTQSYLSAPGT-FPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAG 425
G P + P Q Y PG +P PP PGY P PPAG P PP+ + G
Sbjct: 176 GAYPGYPP------QGYPPQPGHGYPPQHGYPPQPGYPPQGYPPAGYPPQHGYPPAGYPG 337
Query: 426 AFPG 429
G
Sbjct: 338 HSAG 349
>TC15252 similar to UP|Q7PSL3 (Q7PSL3) ENSANGP00000019748 (Fragment),
partial (4%)
Length = 698
Score = 36.6 bits (83), Expect = 0.009
Identities = 21/53 (39%), Positives = 25/53 (46%)
Frame = +2
Query: 380 TQSYLSAPGTFPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAGAFPGSQP 432
T S S P PSG A+ P P P P AG P+S P + AF +QP
Sbjct: 14 TPSASSPPAPTPSGPASGPGPAAGPGPGPAAGPGPNSADTPPPPSAAFSLNQP 172
>TC16883 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase-like
protein, partial (24%)
Length = 602
Score = 36.2 bits (82), Expect = 0.011
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +1
Query: 386 APGTFPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAGAFP 428
APG P+G+ A P P PAAVPP + S PP+S A P
Sbjct: 382 APGAAPTGKNASPNP---PAAVPPPPSPSSSPAPPASSAAMSP 501
>TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-like
protein {Arabidopsis thaliana;}, partial (53%)
Length = 596
Score = 34.7 bits (78), Expect = 0.033
Identities = 24/83 (28%), Positives = 39/83 (46%)
Frame = +1
Query: 350 APAWQTTQAATMYSGSMGQMPSWDPNQQEVTQSYLSAPGTFPSGQAAPPFPGYSPAAVPP 409
AP+ AA+ +S S G S PNQ E + S ++P + ++PP+ P + PP
Sbjct: 145 APSSSPIPAASSFSPSGGPH-SLRPNQSEPSPSCTASPASQAGSSSSPPYTSPKPDS-PP 318
Query: 410 AGASPHSHMPPSSFAGAFPGSQP 432
A ++ P+ + P S P
Sbjct: 319 APSTTRDTASPTGSSRTSPMSTP 387
>BP056337
Length = 550
Score = 34.7 bits (78), Expect = 0.033
Identities = 24/73 (32%), Positives = 36/73 (48%), Gaps = 9/73 (12%)
Frame = +1
Query: 371 SWDPNQQEVTQSY-------LSAPGTFPSG-QAAPPFPGYSPAAVPPAG-ASPHSHMPPS 421
S +PNQQ+ T + + P + P+ ++P FP SP+ PP+ +P S PP
Sbjct: 322 SGNPNQQQQTTHFDFQKLFKATPPPSIPNPLNSSPSFPAPSPSTPPPSSYPTPSSSYPPP 501
Query: 422 SFAGAFPGSQPHY 434
+ G P PHY
Sbjct: 502 T--GTNPYHFPHY 534
>TC15425 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall structural
protein 1.8 precursor (GRP 1.8), partial (6%)
Length = 667
Score = 34.3 bits (77), Expect = 0.043
Identities = 23/68 (33%), Positives = 31/68 (44%), Gaps = 2/68 (2%)
Frame = +2
Query: 368 QMPSWDPNQQEVTQSYLSAPGTFPSGQAA--PPFPGYSPAAVPPAGASPHSHMPPSSFAG 425
Q P + P+Q+ TQ+ S P T P Q+A PP P Y P P + P + A
Sbjct: 5 QTPYYQPDQRSDTQTNPSRPQT-PYYQSAEQPPVPAYGHHPPPYGHQPPPPYQIPPTSAA 181
Query: 426 AFPGSQPH 433
+P Q H
Sbjct: 182 PYPPPQVH 205
>TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%)
Length = 814
Score = 34.3 bits (77), Expect = 0.043
Identities = 18/46 (39%), Positives = 20/46 (43%)
Frame = +2
Query: 390 FPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAGAFPGSQPHYG 435
+P Q PP GY PA PP P + P S A G Q H G
Sbjct: 257 YPPQQGYPPQQGYPPAGYPPQQGYPPAGYPGPSGAHGHGGHQGHGG 394
Score = 34.3 bits (77), Expect = 0.043
Identities = 19/56 (33%), Positives = 23/56 (40%), Gaps = 7/56 (12%)
Frame = +2
Query: 387 PGTFPSGQAAPPFPGYSP-------AAVPPAGASPHSHMPPSSFAGAFPGSQPHYG 435
PG +P PP GY P PPAG P PP+ + G G+ H G
Sbjct: 212 PGAYPPQHGYPPQQGYPPQQGYPPQQGYPPAGYPPQQGYPPAGYPGP-SGAHGHGG 376
>AV411518
Length = 360
Score = 33.5 bits (75), Expect = 0.073
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 1/83 (1%)
Frame = +3
Query: 341 LDPSLHAAQAPAWQTTQAATMYSGSMGQMPSWDPNQQEVTQSYLSAPGTFPSGQAAPPFP 400
L PS A PAW + A+ S P+ S +P PS + P P
Sbjct: 87 LPPSP*AHPTPAWAGSALASWASPCA---PTSSGPASPSPSSTAPSPRPNPSSTSGPTSP 257
Query: 401 G-YSPAAVPPAGASPHSHMPPSS 422
++P+ PP +SP S +PP+S
Sbjct: 258 TPHTPSPPPPTSSSPSSAIPPTS 326
>TC9920 similar to UP|Q9FF85 (Q9FF85) Emb|CAB80929.1, partial (34%)
Length = 825
Score = 33.1 bits (74), Expect = 0.096
Identities = 22/48 (45%), Positives = 26/48 (53%), Gaps = 5/48 (10%)
Frame = +3
Query: 392 SGQAAPPFPGY---SPAAVPPAGASPHSHMPPSSFA--GAFPGSQPHY 434
SG APP Y SP VPPA + +SH PP+ A GA P P+Y
Sbjct: 648 SGYRAPPPGAYYAASPYGVPPAQSRDYSHAPPAYGAPYGA-PPQDPYY 788
>TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase isolog,
25331-24357 (At1g11090), partial (18%)
Length = 552
Score = 33.1 bits (74), Expect = 0.096
Identities = 30/99 (30%), Positives = 41/99 (41%)
Frame = +3
Query: 348 AQAPAWQTTQAATMYSGSMGQMPSWDPNQQEVTQSYLSAPGTFPSGQAAPPFPGYSPAAV 407
A PA + ++A+ + G PS P SA + S + PP SP++
Sbjct: 228 APTPAGSSRRSAS--TSPPGATPSSPPTSSATAAPTASAATSATSTASPPP---RSPSSS 392
Query: 408 PPAGASPHSHMPPSSFAGAFPGSQPHYGWESRFRLNYNP 446
A ASP PSS A + GS P +R R Y P
Sbjct: 393 TSAAASPTPPFRPSSSASPWAGSPPSSCTSNRSR-RYGP 506
>TC19296 similar to UP|O22855 (O22855) At2g43410 protein, partial (6%)
Length = 481
Score = 33.1 bits (74), Expect = 0.096
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = +1
Query: 256 AVPLD--VLHSVFSAFGFVQKVAMFDKNGHTHALIQYPDLTIAAAAKETLEGHCIYDGGY 313
AV +D +LH+ FG ++++ F H AL+++ + A AKE L+G D
Sbjct: 238 AVQIDEQMLHNAMILFGEIERIRSFPSKNH--ALVEFRSVDEARRAKEGLQGRLFNDP-- 405
Query: 314 CKLHLTYS 321
++ +TYS
Sbjct: 406 -RITITYS 426
>TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinogalactan
protein 1 precursor, partial (43%)
Length = 890
Score = 32.7 bits (73), Expect = 0.13
Identities = 26/83 (31%), Positives = 33/83 (39%), Gaps = 1/83 (1%)
Frame = +2
Query: 344 SLHAAQAP-AWQTTQAATMYSGSMGQMPSWDPNQQEVTQSYLSAPGTFPSGQAAPPFPGY 402
S + Q+P A TT T S + Q P + + S SAP + APP P
Sbjct: 314 STNGPQSPSAPSTTPPWTTSSQNTHQSPPSRTSSPSTSSSTTSAPRSSTRSPTAPPSPPQ 493
Query: 403 SPAAVPPAGASPHSHMPPSSFAG 425
P A P S P+S AG
Sbjct: 494 CTKPPAPPRAPPDSSTSPTSAAG 562
>TC7794 homologue to UP|Q9M600 (Q9M600) Chlorophyll a/b binding protein
precursor, partial (97%)
Length = 2581
Score = 32.7 bits (73), Expect = 0.13
Identities = 24/72 (33%), Positives = 32/72 (44%)
Frame = +1
Query: 367 GQMPSWDPNQQEVTQSYLSAPGTFPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAGA 426
GQ + P+ QEV + + T ++ P Y P V G P S PPS G
Sbjct: 442 GQALNLSPSSQEVGRFSMRKAATTKKVVSSGS-PWYGPDRVKYLG--PFSGEPPSYLTGE 612
Query: 427 FPGSQPHYGWES 438
FPG YGW++
Sbjct: 613 FPGD---YGWDT 639
>TC15554 similar to GB|AAO24533.1|27808506|BT003101 At2g41420 {Arabidopsis
thaliana;}, partial (77%)
Length = 679
Score = 32.0 bits (71), Expect = 0.21
Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Frame = +1
Query: 387 PGTFPSGQAAPPFPGYSPAAVPPAGASPHSHMPPSSFAGAFP---GSQPHYGWESRF 440
P +PS A PP PGY PPAG P + P + +P QP E+ F
Sbjct: 127 PQGYPSKDAYPP-PGYPAQGYPPAGYPPQGY-PQQGYPQQYPPQYAQQPQQRQETGF 291
>TC12394 weakly similar to UP|Q9FEV7 (Q9FEV7) Microtubule-associated protein
MAP65-1c, partial (11%)
Length = 445
Score = 32.0 bits (71), Expect = 0.21
Identities = 15/41 (36%), Positives = 23/41 (55%)
Frame = +2
Query: 186 GACNLRISYSAHRDLNIKFQSNRSRDYTNPMLPVNQAAIDS 226
G N R+S +AH++ + + RD T P+ PVN AI +
Sbjct: 56 GTPNRRLSLNAHQNGSRSISKDGKRDNTRPVAPVNYVAISN 178
>TC14775 PIR|S14181|S14181 DNA-directed RNA polymerase largest chain
(isoform B1) - soybean (fragment) {Glycine
max;} , partial (22%)
Length = 848
Score = 31.6 bits (70), Expect = 0.28
Identities = 29/123 (23%), Positives = 56/123 (44%), Gaps = 6/123 (4%)
Frame = +1
Query: 330 AFSDKSRDYTVLDPSLHAAQAPAWQTTQAA-----TMYSGSMGQMPSWDPNQQEVTQSYL 384
A+S S Y+ PS ++ +P++ T + YS S+ PS P +
Sbjct: 25 AYSPTSPGYSPTSPS-YSPTSPSYSPTSPSYNPQSAKYSPSLAYSPS-SPRLSPSSPYSP 198
Query: 385 SAPGTFPSGQA-APPFPGYSPAAVPPAGASPHSHMPPSSFAGAFPGSQPHYGWESRFRLN 443
++P P+ + +P P YSP++ + +SP++ ++ + P P G+ S +
Sbjct: 199 TSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYNSGVSPDYSPSSPQFSPSTGY-SPSQPG 375
Query: 444 YNP 446
Y+P
Sbjct: 376 YSP 384
>TC14213
Length = 1284
Score = 31.6 bits (70), Expect = 0.28
Identities = 37/120 (30%), Positives = 45/120 (36%), Gaps = 2/120 (1%)
Frame = +1
Query: 307 CIYDGGYCKLHLTYSRHTDLNVKAFSDKSRDYTVLDPSLHAAQAPAWQTTQAATMYSGSM 366
C+ D YC T K DK RD + P A PA Q ++ S+
Sbjct: 643 CLADFVYC----TVCACMQTQHKVEMDK-RD-GMFGPQPAMAVPPAQQMSRIDQPVPPSV 804
Query: 367 GQMPSWDPNQQEVTQSYLSAPGTFPSGQ--AAPPFPGYSPAAVPPAGASPHSHMPPSSFA 424
G P P Q Q AP P G A PP GY PPA P + PPS ++
Sbjct: 805 GYAPQPAPYGQPAPQP---APYGQPYGYPPAPPPAQGYPATGYPPAAYPPPGY-PPSGYS 972
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.131 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,896,239
Number of Sequences: 28460
Number of extensions: 116206
Number of successful extensions: 1313
Number of sequences better than 10.0: 149
Number of HSP's better than 10.0 without gapping: 1114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1250
length of query: 449
length of database: 4,897,600
effective HSP length: 93
effective length of query: 356
effective length of database: 2,250,820
effective search space: 801291920
effective search space used: 801291920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148485.4