BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148484.2 - phase: 0
(192 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3,... 42 7e-05
AV418280 39 8e-04
BP080173 34 0.019
AV423569 31 0.12
BP034765 31 0.16
TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular so... 31 0.16
AV411298 30 0.21
CB828953 30 0.27
BI418523 30 0.27
AV427022 29 0.46
AV780445 29 0.60
TC11323 28 0.78
BP046853 27 2.3
TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, part... 27 2.3
TC9793 27 3.0
AV412895 26 3.9
TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyprolin... 26 3.9
TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein... 26 3.9
BP033684 26 5.1
TC17298 similar to UP|Q9AT29 (Q9AT29) bZIP transcription factor,... 26 5.1
>TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3, P1 clone:
MIG10, partial (18%)
Length = 1118
Score = 42.0 bits (97), Expect = 7e-05
Identities = 32/134 (23%), Positives = 59/134 (43%)
Frame = -3
Query: 56 TVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITEWQIANTKTPAESRAATTAPPTQLLSN 115
T+W++WK+RN +++ ++ + T +QI + + A + + +
Sbjct: 981 TLWAIWKNRNRVIFQ---------LEEPNPVNTIFQIRHMQQDANLMSTSQSNKQTTRER 829
Query: 116 SAEVNSSDDGLVLWQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFVLAKMTSFPC 175
+ V S+ D W+ P G YK N DA + + +G+ I D G +++ S P
Sbjct: 828 NINVTSARDR--SWRPPPLGVYKLNSDASWKAPSPSCTVGVVIRDNLG-LLISGTASRPL 658
Query: 176 LHQVTVGEAMGLFE 189
V EA+ L E
Sbjct: 657 APSPIVSEALALRE 616
>AV418280
Length = 398
Score = 38.5 bits (88), Expect = 8e-04
Identities = 22/66 (33%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = -2
Query: 3 HLFFACSFAIQVWRLSGLWSDVSAA-NSNDDSNIG--TVFALLDMLTTKQRVVFAATVWS 59
HLFF C F++ VW+ W +S A ++ +N ++ A + ++ ATVWS
Sbjct: 352 HLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQFSITARNKNQRLGELAIWIATVWS 173
Query: 60 LWKHRN 65
LW RN
Sbjct: 172 LWIQRN 155
>BP080173
Length = 382
Score = 33.9 bits (76), Expect = 0.019
Identities = 19/68 (27%), Positives = 28/68 (40%)
Frame = -2
Query: 121 SSDDGLVLWQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFVLAKMTSFPCLHQVT 180
+S V W P G K N+D F+ + N G + D EG F+ C + +
Sbjct: 369 ASGTTFVRWPVPMEGWIKINIDGSFAPSSNCAACGGVMWDHEGTFITGASVRLGC-YFIN 193
Query: 181 VGEAMGLF 188
E G+F
Sbjct: 192 HAELWGIF 169
>AV423569
Length = 351
Score = 31.2 bits (69), Expect = 0.12
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -1
Query: 30 NDDSNIGTVFALLDMLTTKQRVVFAATVWSLWKHRNLKVWE 70
ND ++ F+ L +TT+ +VVF A VW +W ++ + E
Sbjct: 303 NDTPDVDLGFSHLAPVTTEPKVVFVALVWLVW*EESVSLSE 181
>BP034765
Length = 506
Score = 30.8 bits (68), Expect = 0.16
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = -2
Query: 55 ATVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITEWQIANTK 96
A W+LW+HRN V+ + D+A++ + +W A K
Sbjct: 493 AMAWALWEHRNWIVFNGGLLQEDSIFDKAQLKVCQWLSAKGK 368
>TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular
solute-binding family 3 protein, partial (3%)
Length = 503
Score = 30.8 bits (68), Expect = 0.16
Identities = 21/88 (23%), Positives = 36/88 (40%), Gaps = 2/88 (2%)
Frame = +1
Query: 5 FFACSFAIQVWRLSGLWSDVSAANSNDDSNIGTVFALLDMLTTKQRV--VFAATVWSLWK 62
FF S +++W W V + FA L +L K + V+ A VW +W
Sbjct: 64 FFLLSLGLEIWCRVFNWLGVCFVCPGTPVDHLLSFAGLFLLAQKDGILSVWLAVVWHVWV 243
Query: 63 HRNLKVWEDVTEVAAVVVDRARVMITEW 90
RN V+ + + + ++ + EW
Sbjct: 244 GRNEAVFREGVFLPECIFYLVKLKVWEW 327
>AV411298
Length = 429
Score = 30.4 bits (67), Expect = 0.21
Identities = 9/20 (45%), Positives = 16/20 (80%)
Frame = +1
Query: 50 RVVFAATVWSLWKHRNLKVW 69
RV++ AT W++W+HRN ++
Sbjct: 367 RVIWFATAWTIWRHRNCVIF 426
>CB828953
Length = 554
Score = 30.0 bits (66), Expect = 0.27
Identities = 14/38 (36%), Positives = 19/38 (49%)
Frame = -3
Query: 129 WQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFV 166
W+ P K NVDA + ++ IGI + DE G V
Sbjct: 540 WRPPLPRMLKLNVDASWKASSPLCSIGIAVRDEAGLLV 427
>BI418523
Length = 496
Score = 30.0 bits (66), Expect = 0.27
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = +1
Query: 129 WQKPTSGRYKCNVDAVFSSNFNRTGIGICIHD 160
W P++G KCNVD N +G+G D
Sbjct: 187 WSPPSAGFIKCNVDGASQGNPGPSGVGGVFRD 282
>AV427022
Length = 429
Score = 29.3 bits (64), Expect = 0.46
Identities = 17/71 (23%), Positives = 34/71 (46%), Gaps = 3/71 (4%)
Frame = +1
Query: 2 AHLFFACSFAIQVWRLSGLWSDVSAANSNDDSNIGTVFALLDMLTTK---QRVVFAATVW 58
+HL F+CS ++ + W VS A +D F+ + + + ++ + +W
Sbjct: 73 SHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFSSIGWSKAQKLGESAIWMSVLW 252
Query: 59 SLWKHRNLKVW 69
S+W RN+ V+
Sbjct: 253 SIWCLRNMVVF 285
>AV780445
Length = 504
Score = 28.9 bits (63), Expect = 0.60
Identities = 33/115 (28%), Positives = 45/115 (38%), Gaps = 3/115 (2%)
Frame = +1
Query: 55 ATVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITEWQIANTKTPAESRAATTAPPTQLLS 114
A VW +WK RN V+ED + VD A W+ K E T
Sbjct: 229 AGVW*VWKWRNNMVFED----SPWSVDEA------WR----KLGHEHDEFITF------- 345
Query: 115 NSAEVNSSDDGLVL---WQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFV 166
++ ++ D G L WQ P G K NVD G G I D++G ++
Sbjct: 346 --SDGDAGDPGSWLSSRWQPPRQGTVKLNVDGSGREQDRCMGAGGLIRDDQGKWL 504
>TC11323
Length = 657
Score = 28.5 bits (62), Expect = 0.78
Identities = 13/38 (34%), Positives = 16/38 (41%)
Frame = +2
Query: 129 WQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFV 166
W KP GR+K N D N G G + D F+
Sbjct: 5 WLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFI 118
>BP046853
Length = 612
Score = 26.9 bits (58), Expect = 2.3
Identities = 20/87 (22%), Positives = 40/87 (44%), Gaps = 10/87 (11%)
Frame = -1
Query: 37 TVFALLDMLTTKQRVVFAATVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITE------W 90
T+ L ++ + A+ +WS+W+ RN V++ + + +A +++ W
Sbjct: 570 TILKRLSQDFDREFSLLASLLWSIWRGRNNSVFKAICPDPEFTIQQATRLLSTMDPKLLW 391
Query: 91 QI-ANTKTPAESRA---ATTAPPTQLL 113
+ + T T S+ +TAPP LL
Sbjct: 390 EC*SGTSTLLFSQVPMLRSTAPPPSLL 310
>TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, partial (19%)
Length = 642
Score = 26.9 bits (58), Expect = 2.3
Identities = 32/149 (21%), Positives = 55/149 (36%), Gaps = 18/149 (12%)
Frame = +2
Query: 52 VFAATVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITEWQIANTKTPAESRAATTAPPTQ 111
++A V S ++ ++ +W DVT + + + V +IA + T+P T
Sbjct: 173 IWAKLVPSDSRYSDIVIWSDVTGICSEISATPSVKRCWCKIARNSDLCSATMENTSPNTI 352
Query: 112 LL--------------SNSAEVNSSDDGLVLWQ-KPTSGRYKCNVDAVFSSNFNRTGIGI 156
L+ S ++ SD G V ++ S C + + T I
Sbjct: 353 LVDGAVVCNDETIAIKDGSEIISGSDKGYVSYRFHIMSSPETCQRELKICVDVENTKCSI 532
Query: 157 CI---HDEEGAFVLAKMTSFPCLHQVTVG 182
C+ HD +T+ PCLH G
Sbjct: 533 CLNIWHD--------VVTTGPCLHNFCNG 595
>TC9793
Length = 568
Score = 26.6 bits (57), Expect = 3.0
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = +2
Query: 127 VLWQKPTSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFVLAKMTSFPCLHQVTVGEAMG 186
++W+ P G K N+DA +S + + TG + ++A + T+ EA+
Sbjct: 56 MVWEPPPEGIIKVNIDAGWSGSCS-TGFSLVARSHNAVMLVAATHLEETQIEPTLAEALA 232
Query: 187 L 187
L
Sbjct: 233 L 235
>AV412895
Length = 405
Score = 26.2 bits (56), Expect = 3.9
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +1
Query: 2 AHLFFACSFAIQVWRLSGLWSDVSAANSND 31
+HL F+C+ ++ +W W VS A +D
Sbjct: 238 SHLLFSCAESMLIWYECFAWLGVSTAQVSD 327
>TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyproline-rich
glycoprotein {Arabidopsis thaliana;} , partial (6%)
Length = 678
Score = 26.2 bits (56), Expect = 3.9
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +3
Query: 50 RVVFAATVWSLWKHRNLKVWEDV 72
+VV+ A +W++W RN V+ +V
Sbjct: 585 KVVWLAVIWTVWNGRNNLVFREV 653
>TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein MID2
(Serine-rich protein SMS1) (Protein kinase A
interference protein), partial (7%)
Length = 704
Score = 26.2 bits (56), Expect = 3.9
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = -2
Query: 133 TSGRYKCNVDAVFSSNFNRTGIGICIHDEEGAFVLAK--MTSFPCL 176
T+ +Y CN+D SSN + + I AF+L+K TSFP L
Sbjct: 532 TNNQYYCNLDLRISSNSSSSSI-------LSAFMLSKTPSTSFPHL 416
>BP033684
Length = 557
Score = 25.8 bits (55), Expect = 5.1
Identities = 27/105 (25%), Positives = 37/105 (34%)
Frame = -1
Query: 51 VVFAATVWSLWKHRNLKVWEDVTEVAAVVVDRARVMITEWQIANTKTPAESRAATTAPPT 110
++ A VWSLW R V++ + V D + I W A K E
Sbjct: 512 LILYALVWSLWLERKQSVFKGKLASSQEVWDLHLMTIFWWIKAVWK---ECPYNFDQFHR 342
Query: 111 QLLSNSAEVNSSDDGLVLWQKPTSGRYKCNVDAVFSSNFNRTGIG 155
S + L W P+ G K NVD N +G+G
Sbjct: 341 DFCMLSFKKLIPKQRLKGWTPPSRGILKFNVDGASQGNPGPSGVG 207
>TC17298 similar to UP|Q9AT29 (Q9AT29) bZIP transcription factor, partial
(39%)
Length = 484
Score = 25.8 bits (55), Expect = 5.1
Identities = 14/42 (33%), Positives = 24/42 (56%)
Frame = +2
Query: 84 RVMITEWQIANTKTPAESRAATTAPPTQLLSNSAEVNSSDDG 125
R++ ++ + +TP + AAT Q +SNS +V+SS G
Sbjct: 185 RIIFKHFKTSVLRTPHHASAATQPLMKQRISNSNKVSSSMRG 310
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.130 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,886,717
Number of Sequences: 28460
Number of extensions: 49215
Number of successful extensions: 316
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 311
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 313
length of query: 192
length of database: 4,897,600
effective HSP length: 85
effective length of query: 107
effective length of database: 2,478,500
effective search space: 265199500
effective search space used: 265199500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC148484.2