BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148483.19 - phase: 0
(230 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18452 weakly similar to GB|AAO64875.1|29028992|BT005940 At5g27... 57 3e-09
TC12475 weakly similar to UP|O22512 (O22512) Grr1, partial (23%) 41 2e-04
TC14746 weakly similar to GB|AAG21976.1|10716947|AF263377 SKP1 i... 36 0.005
BI418203 34 0.024
TC12566 similar to UP|Q84XI1 (Q84XI1) RCa1 (Fragment), partial (... 33 0.041
TC13534 similar to UP|Q9FE83 (Q9FE83) Emb|CAB62116.1 (SKP1 inter... 33 0.054
TC15634 similar to UP|Q8LGK0 (Q8LGK0) F-box protein family, AtFB... 33 0.054
TC8778 similar to UP|O22512 (O22512) Grr1, partial (31%) 32 0.092
TC14328 homologue to GB|AAG21976.1|10716947|AF263377 SKP1 intera... 30 0.46
TC9950 similar to UP|AAR08678 (AAR08678) EIN2, partial (11%) 28 1.3
BP049329 28 1.3
BP055837 28 1.7
TC17230 homologue to UP|O23635 (O23635) Transcription factor (Fr... 27 2.3
TC16939 homologue to UP|O23140 (O23140) AP47/50P, partial (32%) 27 3.0
TC8980 similar to UP|Q7X998 (Q7X998) mRNA-binding protein (Fragm... 27 3.0
AV770499 27 3.0
TC15963 similar to GB|AAF24126.1|6690399|AF121673 soluble starch... 27 3.9
TC19321 weakly similar to UP|MT70_ARATH (O82486) Probable N6-ade... 27 3.9
TC18501 26 5.1
BP059240 26 6.6
>TC18452 weakly similar to GB|AAO64875.1|29028992|BT005940 At5g27920
{Arabidopsis thaliana;}, partial (11%)
Length = 698
Score = 57.0 bits (136), Expect = 3e-09
Identities = 48/164 (29%), Positives = 81/164 (49%), Gaps = 13/164 (7%)
Frame = +2
Query: 54 KC--LASLDLLSSNISDELLSSIATERLPLTRLVLQYCIGI----VQVLGICCN-IRHLN 106
KC L LD+ + + DE L SI+ L+ L + C+ I V +G+CC+ ++ L+
Sbjct: 62 KCHYLEELDITDNEVDDEGLKSISNCSR-LSSLKVGICLNITDRGVAYIGMCCSKLKELD 238
Query: 107 LSKCSMVKLEMNFEV-----PKLEVLNLSY-TNVDDEALYMISKSCCGLLKLSLENCNDF 160
L + + + ++ P LE++N SY TN+ D +L +SK C L L + C
Sbjct: 239 LYRSTGIT-DLGISAIACGCPGLEIINTSYCTNITDGSLISLSK-CRNLKTLEIRGCLLV 412
Query: 161 TKKGVNHVVENCTQLRKISLDGCHKVHANIVSSMVSSRPSLRQI 204
T G+ + NC QL + + C+ + + + + SLRQI
Sbjct: 413 TSIGLASIAMNCKQLICVDIKKCYNIDDSGMIPLAYFSKSLRQI 544
>TC12475 weakly similar to UP|O22512 (O22512) Grr1, partial (23%)
Length = 501
Score = 40.8 bits (94), Expect = 2e-04
Identities = 36/133 (27%), Positives = 58/133 (43%), Gaps = 8/133 (6%)
Frame = +2
Query: 81 LTRLVLQYCIGIVQVLGI------CCNIRHLNLSKCSMVKLEMNFEVPKLEVLNLSYTNV 134
L L + C G+ V GI C N++ ++L KC+ +
Sbjct: 95 LKSLTIASCRGVTDV-GIEAVGKGCPNLKSVHLHKCAFLS-------------------- 211
Query: 135 DDEALYMISKSCCGLLKLSLENCNDFTKKGVNHVVENC-TQLRKISLDGCHKV-HANIVS 192
D L +K+ L L LE C+ T+ G V+ NC +L+ IS+ C+ + N+V
Sbjct: 212 -DNGLISFTKAASSLENLQLEECHRITQFGFFGVLFNCGAKLKAISMVSCYGIKDQNLVL 388
Query: 193 SMVSSRPSLRQIT 205
S +S SLR ++
Sbjct: 389 SPISPCESLRSLS 427
Score = 30.4 bits (67), Expect = 0.27
Identities = 16/65 (24%), Positives = 29/65 (44%), Gaps = 1/65 (1%)
Frame = +2
Query: 123 KLEVLNL-SYTNVDDEALYMISKSCCGLLKLSLENCNDFTKKGVNHVVENCTQLRKISLD 181
KL+ L + S V D + + K C L + L C + G+ + + L + L+
Sbjct: 92 KLKSLTIASCRGVTDVGIEAVGKGCPNLKSVHLHKCAFLSDNGLISFTKAASSLENLQLE 271
Query: 182 GCHKV 186
CH++
Sbjct: 272 ECHRI 286
>TC14746 weakly similar to GB|AAG21976.1|10716947|AF263377 SKP1 interacting
partner 1 {Arabidopsis thaliana;}, partial (62%)
Length = 719
Score = 36.2 bits (82), Expect = 0.005
Identities = 23/77 (29%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +3
Query: 102 IRHLNLSKCSMVKLEMNFE-VPKLEVLNL-SYTNVDDEALYMISKSCCGLLKLSLENCND 159
+ + + CS L + E P LEVL++ S +V D+++ I+ +C L +L + C D
Sbjct: 354 LTQIRIRHCSDRSLALVAERCPNLEVLSIRSCPHVTDDSISRIAVNCPKLRELDISYCYD 533
Query: 160 FTKKGVNHVVENCTQLR 176
T K + + NC L+
Sbjct: 534 VTHKSLALIGRNCPNLK 584
>BI418203
Length = 681
Score = 33.9 bits (76), Expect = 0.024
Identities = 19/59 (32%), Positives = 31/59 (52%)
Frame = +3
Query: 122 PKLEVLNLSYTNVDDEALYMISKSCCGLLKLSLENCNDFTKKGVNHVVENCTQLRKISL 180
P L+ L + +DD A+ ++ K L +L L NC DF+ K ++ + C LR + L
Sbjct: 195 PYLQSLKVDCGRLDDSAIALLVKP--SLHELCLHNCADFSGKLLSEIGTRCKDLRSLYL 365
>TC12566 similar to UP|Q84XI1 (Q84XI1) RCa1 (Fragment), partial (28%)
Length = 918
Score = 33.1 bits (74), Expect = 0.041
Identities = 27/104 (25%), Positives = 47/104 (44%), Gaps = 10/104 (9%)
Frame = +1
Query: 79 LPLTRLVLQYCIGI---VQVLGICCNIRHLNLSKCSMVKLEMNFEVPKLEVLNLSYTNVD 135
+ L++ +L Y GI ++VLG+ R + +VKLE + + VL LSY +D
Sbjct: 100 IDLSKKLLSYAKGIPLALKVLGLFLYGRERKAWESQLVKLEKLPDPEIINVLKLSYDGLD 279
Query: 136 DEALYMISKSCCGLL-------KLSLENCNDFTKKGVNHVVENC 172
D+ + C L +L++C F G+ + + C
Sbjct: 280 DDQKDIFLDIACFYLGHLEESVAQTLDSCGFFADIGMEVLKDRC 411
>TC13534 similar to UP|Q9FE83 (Q9FE83) Emb|CAB62116.1 (SKP1 interacting
partner 2) (SKIP2) (At5g67250), partial (27%)
Length = 524
Score = 32.7 bits (73), Expect = 0.054
Identities = 19/56 (33%), Positives = 28/56 (49%)
Frame = +1
Query: 127 LNLSYTNVDDEALYMISKSCCGLLKLSLENCNDFTKKGVNHVVENCTQLRKISLDG 182
++L V D L +SK C L L L + + G++ V E C LRK+ +DG
Sbjct: 283 IHLEKVQVSDVGLAGVSK-CFNLETLHLVKTPECSDSGLSGVAERCKMLRKLHIDG 447
>TC15634 similar to UP|Q8LGK0 (Q8LGK0) F-box protein family, AtFBL4, partial
(13%)
Length = 613
Score = 32.7 bits (73), Expect = 0.054
Identities = 15/59 (25%), Positives = 35/59 (58%), Gaps = 1/59 (1%)
Frame = +1
Query: 147 CGLLK-LSLENCNDFTKKGVNHVVENCTQLRKISLDGCHKVHANIVSSMVSSRPSLRQI 204
C L++ + + +C T G+ H+V++CT L + C + + V+++VSS +++++
Sbjct: 1 CPLIREIVISHCRQITDVGLAHLVKSCTMLESCHMVYCSGITSAGVATVVSSCLNIKKV 177
>TC8778 similar to UP|O22512 (O22512) Grr1, partial (31%)
Length = 877
Score = 32.0 bits (71), Expect = 0.092
Identities = 27/119 (22%), Positives = 54/119 (44%), Gaps = 5/119 (4%)
Frame = +3
Query: 82 TRLVLQYCIGIVQVLGICCNIRHLNLSKCSMVK----LEMNFEVPKLEVLNLSYTNVDDE 137
T+L Q + +V++ G C++ LNL+ C + + + P L L++S + D
Sbjct: 432 TKLTDQVVLSLVKLHG--CSLEVLNLNGCKKISDASLIAIAGSCPLLSDLDVSRCGITDT 605
Query: 138 ALYMISKSCCGLLK-LSLENCNDFTKKGVNHVVENCTQLRKISLDGCHKVHANIVSSMV 195
+ ++ LK LSL C + KGV + + + L +++ C + + +V
Sbjct: 606 GIAALAHGKQLNLKVLSLAGCTSVSNKGVRALKKLGSSLDGLNIKNCKGISYRSLDMLV 782
Score = 28.5 bits (62), Expect = 1.0
Identities = 15/54 (27%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +3
Query: 134 VDDEALYMISKSCCGLLKLSLENCNDFTKKGVNHVVENC-TQLRKISLDGCHKV 186
+ D L +K+ + L LE C+ T+ G ++ NC +L+ +SL C+ +
Sbjct: 39 LSDNGLVSFAKAAPSIESLYLEECHRITQLGFFGLLFNCAAKLKVLSLASCYGI 200
>TC14328 homologue to GB|AAG21976.1|10716947|AF263377 SKP1 interacting
partner 1 {Arabidopsis thaliana;}, partial (5%)
Length = 620
Score = 29.6 bits (65), Expect = 0.46
Identities = 13/45 (28%), Positives = 23/45 (50%)
Frame = +2
Query: 163 KGVNHVVENCTQLRKISLDGCHKVHANIVSSMVSSRPSLRQITAP 207
KG+N + + C L + L GC + + +++ SS L+ I P
Sbjct: 62 KGLNLICQRCPNLEFLDLSGCANLTSRDIANASSSLAHLKDIKKP 196
>TC9950 similar to UP|AAR08678 (AAR08678) EIN2, partial (11%)
Length = 853
Score = 28.1 bits (61), Expect = 1.3
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 2/85 (2%)
Frame = +3
Query: 119 FEVPKLEVLNLSYTNVDDEALYMISKSCCGL-LKLSLENCNDFTKKGVNHVVENCTQLRK 177
+EV E+ +S+ EA Y S G +K + NC+ F+ + H E C
Sbjct: 588 YEVETTEMNRVSHMG---EARYFSSDGKSGSSMKNNELNCSSFSVSSIPHCGEGCVWRAD 758
Query: 178 ISLD-GCHKVHANIVSSMVSSRPSL 201
+ + G +H + S++ SRP L
Sbjct: 759 LIISFGVWCIHRVLDLSLMESRPEL 833
>BP049329
Length = 511
Score = 28.1 bits (61), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (55%)
Frame = -3
Query: 100 CNIRHLNLSKCSMVKLEMNFEVPKLEVLNLSY 131
C+I H N+S +VK+ N V E + +S+
Sbjct: 131 CSIEHFNMSLIKLVKIAYNLRVKYFEFIFISF 36
>BP055837
Length = 528
Score = 27.7 bits (60), Expect = 1.7
Identities = 27/95 (28%), Positives = 47/95 (49%), Gaps = 2/95 (2%)
Frame = -1
Query: 111 SMVKLEMNFEVPKLEVLNLSYTNVDDE--ALYMISKSCCGLLKLSLENCNDFTKKGVNHV 168
S+V+ E EV LE++ Y ++++E AL I+ +C + + ++HV
Sbjct: 357 SVVREEWTAEVFDLELMR--YKDIEEEMVALLQIAVACTAAVPDQ--------RPRMSHV 208
Query: 169 VENCTQLRKISLDGCHKVHANIVSSMVSSRPSLRQ 203
V+ +LR + + CH + V S VS PSL +
Sbjct: 207 VKMIEELRGVQVSPCH----DTVDS-VSESPSLSE 118
>TC17230 homologue to UP|O23635 (O23635) Transcription factor (Fragment),
partial (37%)
Length = 554
Score = 27.3 bits (59), Expect = 2.3
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = +1
Query: 175 LRKISLDGCHKVHANIVSSMVSSRPSLRQITAPPTRTGA 213
LR I L GC H + + S+ P + +TAPP G+
Sbjct: 118 LRWILLLGCMFSHHHRLGSLFGRLPPMTALTAPPAPVGS 234
>TC16939 homologue to UP|O23140 (O23140) AP47/50P, partial (32%)
Length = 525
Score = 26.9 bits (58), Expect = 3.0
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +1
Query: 47 FISSLSLKCLASLDLLSSNISDELLSSIAT 76
F+ +L L+C L L +S+ S E+ SSIA+
Sbjct: 85 FVHTLDLRCRWLLPLYTSSTSAEMFSSIAS 174
>TC8980 similar to UP|Q7X998 (Q7X998) mRNA-binding protein (Fragment),
partial (40%)
Length = 598
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/48 (31%), Positives = 24/48 (49%)
Frame = +1
Query: 149 LLKLSLENCNDFTKKGVNHVVENCTQLRKISLDGCHKVHANIVSSMVS 196
+L L++EN + NH + NC R ++LDG K+ A V+
Sbjct: 118 MLTLAVENPD-----AANHTIFNCVSDRAVTLDGMAKLCAQAAGRPVN 246
>AV770499
Length = 491
Score = 26.9 bits (58), Expect = 3.0
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 167 HVVENCTQLRKISLDGCHKVHANIVSSMVSSRPS 200
H V + I++ G H VHAN +S V RPS
Sbjct: 380 HGVNGYGKTEPINILGFHDVHANNLSIHVEKRPS 481
>TC15963 similar to GB|AAF24126.1|6690399|AF121673 soluble starch synthase
{Arabidopsis thaliana;} , partial (24%)
Length = 699
Score = 26.6 bits (57), Expect = 3.9
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -1
Query: 86 LQYCIGIVQVLGICCNIRHLNLSKCSMVKLE 116
L Y VQ IC I +L KC MVKL+
Sbjct: 633 LSYYQSTVQDRNICAAINKNSLKKCLMVKLQ 541
>TC19321 weakly similar to UP|MT70_ARATH (O82486) Probable
N6-adenosine-methyltransferase MT-A70-like protein ,
partial (8%)
Length = 541
Score = 26.6 bits (57), Expect = 3.9
Identities = 20/47 (42%), Positives = 23/47 (48%)
Frame = -2
Query: 35 KVRYESAYTTSHFISSLSLKCLASLDLLSSNISDELLSSIATERLPL 81
K+RY Y T H SL L AS LL NI + S + T RL L
Sbjct: 324 KIRYYGLYITKHLHVSLVLGIDASFKLL-PNIFNGSYSILITIRLSL 187
>TC18501
Length = 591
Score = 26.2 bits (56), Expect = 5.1
Identities = 10/44 (22%), Positives = 24/44 (53%), Gaps = 2/44 (4%)
Frame = -1
Query: 134 VDDEALYMISKSC--CGLLKLSLENCNDFTKKGVNHVVENCTQL 175
+ D ++Y ++ C ++++ L CN F +H+ ++C Q+
Sbjct: 150 IKDNSIYALNSFVVICNIVEIHLLTCNFFPIGSGSHLFDSCAQI 19
>BP059240
Length = 365
Score = 25.8 bits (55), Expect = 6.6
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -1
Query: 68 DELLSSIATERLPLTRLVLQYCIGIVQVLGICC 100
+ LLSS + L L++ C+ + VL +CC
Sbjct: 254 ETLLSSTCVPYILLFWLIVLSCLDAISVLNLCC 156
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.136 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,460,225
Number of Sequences: 28460
Number of extensions: 60290
Number of successful extensions: 463
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 462
length of query: 230
length of database: 4,897,600
effective HSP length: 87
effective length of query: 143
effective length of database: 2,421,580
effective search space: 346285940
effective search space used: 346285940
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 54 (25.4 bits)
Medicago: description of AC148483.19