BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148482.6 - phase: 0
(402 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14164 weakly similar to UP|Q8VYH6 (Q8VYH6) AT4g17280/dl4675c, ... 607 e-174
TC17875 weakly similar to UP|Q8VYH6 (Q8VYH6) AT4g17280/dl4675c, ... 145 1e-35
AV424389 140 4e-34
TC12675 similar to UP|Q8HBE5 (Q8HBE5) Cytochrome b (Fragment), p... 85 1e-22
TC19486 similar to UP|Q9SJ74 (Q9SJ74) Expressed protein, partial... 100 3e-22
AU251853 100 6e-22
TC8982 similar to UP|Q84QW4 (Q84QW4) OJ1191_A10.2 protein, parti... 76 9e-15
AV771599 50 9e-07
TC14165 41 4e-04
TC13507 37 0.004
BF177747 37 0.008
BP055490 33 0.11
AV778440 30 0.93
TC10233 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (32%) 29 1.2
AV777294 28 2.7
AV781302 28 3.6
TC11753 weakly similar to AAS55571 (AAS55571) At3g09690, partial... 28 3.6
TC17008 similar to UP|Q9LK21 (Q9LK21) Genomic DNA, chromosome 3,... 28 3.6
BG662473 27 4.6
>TC14164 weakly similar to UP|Q8VYH6 (Q8VYH6) AT4g17280/dl4675c, partial
(44%)
Length = 1606
Score = 607 bits (1565), Expect = e-174
Identities = 295/397 (74%), Positives = 338/397 (84%), Gaps = 1/397 (0%)
Frame = +2
Query: 6 RFMFLISILMFVTTTLAQTTTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQTTGKLD 65
RF+ IS+L V T ++ QTC SQ FTNN FTSCRDLP LTSYLHWT+DQ TGKLD
Sbjct: 131 RFVLAISVLCSVFLT---SSAQTCNSQTFTNNKQFTSCRDLPHLTSYLHWTFDQATGKLD 301
Query: 66 IAFRHKGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSSGTPKAYTSSIANSRTQLA 125
IAFRH GIT T++WV+WAINP+++L S+M GAQALVAI QSSG+PK YT+SIAN +A
Sbjct: 302 IAFRHTGITSTDKWVSWAINPSSNLNSAMVGAQALVAIPQSSGSPKVYTTSIANYDPNMA 481
Query: 126 ESNISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQ 185
E NISYPH+GL AT+ N+E+TIYA++TLP GT SLVHLWQDGA+SGST Q H +AN Q
Sbjct: 482 EGNISYPHTGLSATYSNSELTIYATLTLPSGTTSLVHLWQDGAVSGSTLQAHARGTANLQ 661
Query: 186 SKESLDLRSGASEQGSGGGSLSRRRNTHGVLNAISWGILMPLGAVIARYLKVFKSADPAW 245
+KESLDL SG ++ GS G S+ RRRN HGVLN +SWGILMPLGAVIARYLKVFKSADPAW
Sbjct: 662 AKESLDLASGTTQAGSSGNSVRRRRNVHGVLNTVSWGILMPLGAVIARYLKVFKSADPAW 841
Query: 246 FYLHVTCQSAAYIVGVAGWGTGLKLGSDSAGVTYSTHRTLGIVIFCLGTLQVFALLLRPK 305
FYLHVTCQ+AAYIVGVAGWGTGLKLGSDSAG+ YSTHR LGI +FCLGTLQVFALLLRP
Sbjct: 842 FYLHVTCQTAAYIVGVAGWGTGLKLGSDSAGIEYSTHRALGITLFCLGTLQVFALLLRPN 1021
Query: 306 KDHKIRFYWNLYHWGVGYATIIISIINIFKGFEALEVSAADRYDNWKHAYTGIIAALGGV 365
KDHKIRFYWNLYHWG+GYATIIISI+NIFKGF+A+E S DRYD+WK+AY GIIAALGG+
Sbjct: 1022KDHKIRFYWNLYHWGIGYATIIISIVNIFKGFDAMEKSVGDRYDDWKNAYIGIIAALGGI 1201
Query: 366 AVLLEAYTWIIVIKRKKSENKLQGMNGTNG-NGYGSR 401
AVLLE YTWIIV+KR+KSEN++ +G NG NGYGSR
Sbjct: 1202AVLLEVYTWIIVLKRRKSENQM--AHGANGVNGYGSR 1306
>TC17875 weakly similar to UP|Q8VYH6 (Q8VYH6) AT4g17280/dl4675c, partial
(19%)
Length = 567
Score = 145 bits (366), Expect = 1e-35
Identities = 69/98 (70%), Positives = 82/98 (83%), Gaps = 1/98 (1%)
Frame = +1
Query: 299 ALLLRPKKDHKIRFYWNLYHWGVGYATIIISIINIFKGFEALEVSAADRYDNWKHAYTGI 358
AL LR KDHK RFYWN+YH +GY+TIIISIIN+FKGF+ LE DRYDNWK+AY GI
Sbjct: 1 ALFLRLNKDHKHRFYWNIYHHLIGYSTIIISIINVFKGFDTLENYVGDRYDNWKNAYIGI 180
Query: 359 IAALGGVAVLLEAYTWIIVIKRKKSENKL-QGMNGTNG 395
IAALGG+AVLLEAYTWIIV+KR++SE+K G+NG +G
Sbjct: 181 IAALGGIAVLLEAYTWIIVLKRRQSESKTSHGINGAHG 294
>AV424389
Length = 309
Score = 140 bits (353), Expect = 4e-34
Identities = 72/101 (71%), Positives = 84/101 (82%), Gaps = 1/101 (0%)
Frame = +2
Query: 64 LDIAFRHKGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSS-GTPKAYTSSIANSRT 122
LDIA+RH GIT TNRWVAWAINPNN L +M GAQALVAI QSS G+P+AYTSSIA++ T
Sbjct: 5 LDIAYRHTGITSTNRWVAWAINPNNKLNPAMVGAQALVAIPQSSDGSPRAYTSSIASTNT 184
Query: 123 QLAESNISYPHSGLIATHENNEVTIYASITLPVGTPSLVHL 163
QL E ISYP SGL A ++NNEVTI+A++TLP GT SLVH+
Sbjct: 185 QLQEGTISYPVSGLSAEYQNNEVTIFATLTLPNGTTSLVHV 307
>TC12675 similar to UP|Q8HBE5 (Q8HBE5) Cytochrome b (Fragment), partial (5%)
Length = 524
Score = 84.7 bits (208), Expect(2) = 1e-22
Identities = 40/68 (58%), Positives = 53/68 (77%), Gaps = 2/68 (2%)
Frame = +3
Query: 4 NLRFMFLISIL--MFVTTTLAQTTTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQTT 61
NL+ + +SIL + +TT+LAQT CK+Q F+ N +FT+CRDLP+LTSYLHW+YDQ T
Sbjct: 273 NLKLVLALSILSSVLLTTSLAQT----CKTQTFSTNKVFTTCRDLPELTSYLHWSYDQAT 440
Query: 62 GKLDIAFR 69
G LDIA+R
Sbjct: 441 GNLDIAYR 464
Score = 38.5 bits (88), Expect(2) = 1e-22
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1
Query: 72 GITDTNRWVAWAINPNN 88
GIT TNRWV WAINPNN
Sbjct: 472 GITSTNRWVDWAINPNN 522
>TC19486 similar to UP|Q9SJ74 (Q9SJ74) Expressed protein, partial (31%)
Length = 510
Score = 100 bits (250), Expect = 3e-22
Identities = 47/132 (35%), Positives = 79/132 (59%), Gaps = 1/132 (0%)
Frame = +1
Query: 267 GLKLGSDSAGVTYSTHRTLGIVIFCLGTLQVFALLLRPKKDHKIRFYWNLYHWGVGYATI 326
G++LG S GV Y HR LGI +FCLG +Q ALL RP + ++ R YW YH VGY+ +
Sbjct: 4 GIRLGELSPGVEYRLHRKLGIAVFCLGAMQTLALLFRPNERNRFRKYWKSYHHFVGYSCV 183
Query: 327 IISIINIFKGFEALEVSAADRYDNWKHAYTGIIAALGGVAVLLEAYTWIIVIKRKKSEN- 385
++ +N+F+GFE + S + K +Y ++ + GV + LE +W++ ++ K +
Sbjct: 184 VLGFVNVFQGFEVMGASRSYA----KLSYCLGLSTMIGVCIALEVNSWVVFCRKSKEDKM 351
Query: 386 KLQGMNGTNGNG 397
+ +G+ G++ G
Sbjct: 352 RREGLIGSSDKG 387
>AU251853
Length = 286
Score = 100 bits (248), Expect = 6e-22
Identities = 48/91 (52%), Positives = 58/91 (62%), Gaps = 1/91 (1%)
Frame = +3
Query: 1 MVKNLRFMFLISILMFVTTTLAQTTTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQT 60
M+ + F F + + +TTT A T T C F N +F +CRDLP S+LHWTY
Sbjct: 15 MLMRVAFCFSVFTSLILTTT-AHTQTCRCSKYTFPNKEVFATCRDLPHSPSFLHWTYQPA 191
Query: 61 TGKLDIAFRHKGITD-TNRWVAWAINPNNDL 90
TG LDIAFRH GI + TN WVAWAINPNN+L
Sbjct: 192 TGNLDIAFRHSGIENPTNTWVAWAINPNNNL 284
>TC8982 similar to UP|Q84QW4 (Q84QW4) OJ1191_A10.2 protein, partial (10%)
Length = 901
Score = 76.3 bits (186), Expect = 9e-15
Identities = 55/206 (26%), Positives = 90/206 (42%), Gaps = 1/206 (0%)
Frame = +2
Query: 8 MFLISILMFVTTTLAQTTTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQTTGKLDIA 67
+F ISI +F++ + C +Q + + SC +L L + LH+TY++T + +A
Sbjct: 20 IFAISISIFISLFSPTHSAINCATQKLPSGRNYASCTNLSSLGATLHFTYNETNSSIAVA 199
Query: 68 FRHKGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSSGTPKAYTSSIANSRTQLAE- 126
F WVAW +N M G QALVA S ++LAE
Sbjct: 200 F--SAAPPKGGWVAWGLNL---AGGGMAGTQALVAYKGGDKNVVGVHLYNITSYSELAEV 364
Query: 127 SNISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQS 186
+ S+ L A + +TI+A++ +P ++ +WQ G + HD N +
Sbjct: 365 KSFSFETWDLSAEESSAAITIFAAVKVPENADNISQIWQVGPVVSGKINKHDFKPENLAA 544
Query: 187 KESLDLRSGASEQGSGGGSLSRRRNT 212
K L + + + G GG + S NT
Sbjct: 545 KAPLSVVATTAVVG-GGNTTSGSGNT 619
>AV771599
Length = 535
Score = 49.7 bits (117), Expect = 9e-07
Identities = 20/28 (71%), Positives = 25/28 (88%)
Frame = -3
Query: 328 ISIINIFKGFEALEVSAADRYDNWKHAY 355
ISI+NIFKGF+A+E S DRYD+WK+AY
Sbjct: 533 ISIVNIFKGFDAMEKSVGDRYDDWKYAY 450
>TC14165
Length = 431
Score = 40.8 bits (94), Expect = 4e-04
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = +3
Query: 355 YTGIIAALGGVAVLLEAYTWIIVIKRKK 382
Y GIIAALGG+AVLLE YTWI ++ ++
Sbjct: 3 YIGIIAALGGLAVLLEVYTWIPCLEEEE 86
>TC13507
Length = 413
Score = 37.4 bits (85), Expect = 0.004
Identities = 17/49 (34%), Positives = 29/49 (58%)
Frame = +3
Query: 349 DNWKHAYTGIIAALGGVAVLLEAYTWIIVIKRKKSENKLQGMNGTNGNG 397
+ ++ Y ++ LG + ++LE TWI+V+KRK +K +G N NG
Sbjct: 9 EKYRAIYLDVLLILGALVLVLEVLTWIVVLKRKYC-SKSNTYDGYNNNG 152
>BF177747
Length = 264
Score = 36.6 bits (83), Expect = 0.008
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = +2
Query: 135 GLIAT-HENNEVTIYASITLPVGTPSLVHLWQDGA-MSGSTPQMHDMTSANTQSKESLDL 192
G +AT H + IYA+I L + ++W G + G +P +H TS++ S + D+
Sbjct: 20 GKMATIHNGATIQIYATIKLQPNRTKIHYVWNRGLYVQGYSPTIHPTTSSDLSSTATFDV 199
Query: 193 RSGAS 197
SG+S
Sbjct: 200 LSGSS 214
>BP055490
Length = 547
Score = 32.7 bits (73), Expect = 0.11
Identities = 30/111 (27%), Positives = 47/111 (42%)
Frame = -1
Query: 141 ENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQSKESLDLRSGASEQG 200
+NN ++ Y S + PS+ HL+ ++ + Q + + S SL LRS AS
Sbjct: 388 KNNFLSNYYSPSTYSSNPSIAHLFTAPSVQNGSLQSSPSLTFSQVSLPSLHLRSPASLSF 209
Query: 201 SGGGSLSRRRNTHGVLNAISWGILMPLGAVIARYLKVFKSADPAWFYLHVT 251
S S R +PL ++++ F S P +FYLH T
Sbjct: 208 SPSPSNPSR---------------LPL----PKFVEKFNSKSPNFFYLHFT 113
>AV778440
Length = 489
Score = 29.6 bits (65), Expect = 0.93
Identities = 25/79 (31%), Positives = 38/79 (47%), Gaps = 4/79 (5%)
Frame = -1
Query: 86 PNNDLASSMNGAQALVAILQSSGTPKAYTSSIANSRTQL----AESNISYPHSGLIATHE 141
P D+ S+ + L A+ + + A SI SR +L E N P+SG++ E
Sbjct: 351 PVADVESASVHLKHLAAVAKDA---VAEGGSIPGSRAKLWRRIREFNACIPYSGVLPNIE 181
Query: 142 NNEVTIYASITLPVGTPSL 160
EVT+ A IT+ P+L
Sbjct: 180 VPEVTLMALITMLPAAPNL 124
>TC10233 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (32%)
Length = 889
Score = 29.3 bits (64), Expect = 1.2
Identities = 12/39 (30%), Positives = 22/39 (55%)
Frame = -2
Query: 277 VTYSTHRTLGIVIFCLGTLQVFALLLRPKKDHKIRFYWN 315
V + HR LG + CLG + + +L++ P+ + + Y N
Sbjct: 753 VANTRHRALGYITSCLGNIAILSLIICPQLINTLPQYLN 637
>AV777294
Length = 628
Score = 28.1 bits (61), Expect = 2.7
Identities = 15/45 (33%), Positives = 27/45 (59%), Gaps = 3/45 (6%)
Frame = +3
Query: 77 NRWVAWAINPNNDLASSMNGAQALVAI---LQSSGTPKAYTSSIA 118
++W+A ++ L S A+ + A L++SGTPK+YT ++A
Sbjct: 354 SKWIASILSNRIALPSQEEMAKDIEAFYVSLEASGTPKSYTHNLA 488
>AV781302
Length = 533
Score = 27.7 bits (60), Expect = 3.6
Identities = 31/134 (23%), Positives = 53/134 (39%), Gaps = 1/134 (0%)
Frame = -3
Query: 119 NSRTQLAESNISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHD 178
N Q ++S + PH + E TI+ ++PV TP+L + G+ P
Sbjct: 378 NHNNQFSDSRYNQPH-------RDGEGTIHHHHSIPVATPALRGRFL--RRRGAIPATDQ 226
Query: 179 MTSANTQSKESLDLRSGASEQGSGGGSLSRRRNTHGVLNAISW-GILMPLGAVIARYLKV 237
+A ++ GA GS + + R + N + + G+ + L A V
Sbjct: 225 TATATRRTGPLYGRNRGARGFGSESETQNGRFRSRVCENGLGFSGVSLSLSLSHAHTGSV 46
Query: 238 FKSADPAWFYLHVT 251
F +FY+H T
Sbjct: 45 F-----LFFYIHTT 19
>TC11753 weakly similar to AAS55571 (AAS55571) At3g09690, partial (9%)
Length = 630
Score = 27.7 bits (60), Expect = 3.6
Identities = 11/43 (25%), Positives = 26/43 (59%)
Frame = -3
Query: 2 VKNLRFMFLISILMFVTTTLAQTTTQTCKSQNFTNNAIFTSCR 44
V+ L+ +++ I +V+ + + T TCK+ + +++ +SCR
Sbjct: 268 VEYLQPTYVVYISSYVSFHIRNSQTDTCKTSSLVSSSAVSSCR 140
>TC17008 similar to UP|Q9LK21 (Q9LK21) Genomic DNA, chromosome 3, TAC
clone:K17E12, partial (11%)
Length = 516
Score = 27.7 bits (60), Expect = 3.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 235 LKVFKSADPAWFYLHVTCQSAAYI 258
L V K D WF+L TCQSA ++
Sbjct: 286 LMVDKVLDVDWFHLGTTCQSAIFL 357
>BG662473
Length = 434
Score = 27.3 bits (59), Expect = 4.6
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Frame = +2
Query: 366 AVLLEAYTW---IIVIKRKKSENKLQGMNGTNGNGYGS 400
A+ +EA+ W I+ K+ + + NG NG+GYGS
Sbjct: 320 ALEMEAFDWDCGILGDAPKRISDGFEVYNGVNGSGYGS 433
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.133 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,136,526
Number of Sequences: 28460
Number of extensions: 97007
Number of successful extensions: 548
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 544
length of query: 402
length of database: 4,897,600
effective HSP length: 92
effective length of query: 310
effective length of database: 2,279,280
effective search space: 706576800
effective search space used: 706576800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148482.6