BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148469.4 - phase: 0 /pseudo
(312 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19803 similar to UP|O65568 (O65568) Serine carboxypeptidase II... 70 6e-13
TC11375 similar to UP|Q8L7B2 (Q8L7B2) Serine carboxypeptidase 1-... 60 4e-10
AW720368 52 1e-07
TC8804 similar to UP|Q8L6A7 (Q8L6A7) Carboxypeptidase type III, ... 49 1e-06
TC15868 weakly similar to UP|KEX1_YEAST (P09620) Carboxypeptidas... 36 0.007
BP042048 29 0.90
TC14107 homologue to UP|Q42966 (Q42966) Nitrilase , partial (39%) 29 1.2
TC13907 similar to UP|Q94JR4 (Q94JR4) AT4g29590/T16L4_100, parti... 28 2.0
TC11855 similar to UP|Q9C5V6 (Q9C5V6) Topoisomerase 6 subunit B,... 28 2.6
AV426950 27 3.4
TC14106 similar to UP|Q42966 (Q42966) Nitrilase , partial (87%) 27 3.4
TC8589 UP|Q40210 (Q40210) RAB5B, complete 27 3.4
TC14902 27 4.4
TC7983 weakly similar to GB|AAM19921.1|20453365|AY097405 AT3g236... 27 4.4
TC13554 27 5.8
TC17920 26 7.6
AV428764 26 9.9
>TC19803 similar to UP|O65568 (O65568) Serine carboxypeptidase II - like
protein (Serine carboxypeptidase II-like protein),
partial (36%)
Length = 593
Score = 69.7 bits (169), Expect = 6e-13
Identities = 33/60 (55%), Positives = 43/60 (71%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L +++PAGVGFSYS +SD D++TA D LIFL W +F +Y+ DFFITGESYA
Sbjct: 413 ILSVDAPAGVGFSYSNTSSDLLNHGDKKTAEDSLIFLLKWFERFPQYKGRDFFITGESYA 592
>TC11375 similar to UP|Q8L7B2 (Q8L7B2) Serine carboxypeptidase 1-like
protein (Serine carboxypeptidase 1 precursor-like
protein), partial (35%)
Length = 874
Score = 60.5 bits (145), Expect = 4e-10
Identities = 32/70 (45%), Positives = 48/70 (67%), Gaps = 1/70 (1%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDE-RTARDVLIFLQGWVTKFQKYQNSDFFITGESY 59
++YL+SPAGVG SYS N+S Y VTD+ +TA D FL W +F ++ + F+++GESY
Sbjct: 474 IIYLDSPAGVGLSYSKNSSKY--VTDDLQTASDTHAFLFKWFEQFPEFLANPFYLSGESY 647
Query: 60 AVWNLPLITI 69
A +P ++I
Sbjct: 648 AGVYVPTLSI 677
>AW720368
Length = 570
Score = 52.4 bits (124), Expect = 1e-07
Identities = 25/67 (37%), Positives = 41/67 (60%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+++ P G GFSY+ + SD +E + D+ FLQ + + ++ +DFFITGESYA
Sbjct: 155 ILFVDQPTGTGFSYTTDESD-IRHDEEGVSNDLYDFLQAFFKEHSQFTKNDFFITGESYA 331
Query: 61 VWNLPLI 67
+P +
Sbjct: 332 GHYIPAL 352
>TC8804 similar to UP|Q8L6A7 (Q8L6A7) Carboxypeptidase type III, partial
(63%)
Length = 1210
Score = 48.5 bits (114), Expect = 1e-06
Identities = 25/59 (42%), Positives = 35/59 (58%)
Frame = +3
Query: 7 PAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYAVWNLP 65
P G GFSYS + D + + D+ FLQ + T+ +Y ++DFFITGESYA +P
Sbjct: 3 PTGTGFSYSTDKRD-IRHDEAGVSNDLYDFLQAFFTEHPEYASNDFFITGESYAGHYIP 176
>TC15868 weakly similar to UP|KEX1_YEAST (P09620) Carboxypeptidase KEX1
precursor (Carboxypeptidase D) , partial (8%)
Length = 780
Score = 36.2 bits (82), Expect = 0.007
Identities = 20/67 (29%), Positives = 35/67 (51%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+L+SP G GFS T + A+ + ++G++ +++ +ITGESYA
Sbjct: 395 LLFLDSPIGTGFSVGT-TPEEIPADQTGVAKHLYAAIKGFIQLDPVFKDRPIYITGESYA 571
Query: 61 VWNLPLI 67
+P I
Sbjct: 572 GKYVPAI 592
>BP042048
Length = 520
Score = 29.3 bits (64), Expect = 0.90
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 38 QGWVTKFQKYQNSDFFITGESYAVWNLPLI 67
Q + + ++ +DFFITGESYA +P +
Sbjct: 137 QAFFKEHSQFTKNDFFITGESYAGHYIPAL 226
>TC14107 homologue to UP|Q42966 (Q42966) Nitrilase , partial (39%)
Length = 666
Score = 28.9 bits (63), Expect = 1.2
Identities = 16/28 (57%), Positives = 16/28 (57%)
Frame = +1
Query: 193 SSTTGRILRIRLYLYWGDLLGPGYELWC 220
SSTT L IRL W LLG G LWC
Sbjct: 280 SSTTLLPLSIRLRDCWLKLLGLGQSLWC 363
>TC13907 similar to UP|Q94JR4 (Q94JR4) AT4g29590/T16L4_100, partial (9%)
Length = 513
Score = 28.1 bits (61), Expect = 2.0
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +1
Query: 86 TGCCGLSATTPKL*VKT*MGHLVPFVIECFISLLLRLVHLLIL 128
T C +S +T L + *+ L+PF C +S+ L +V LIL
Sbjct: 211 TSLCNVSYSTKVLPLLK*IEGLMPFTFFCRLSIYLLIVDALIL 339
>TC11855 similar to UP|Q9C5V6 (Q9C5V6) Topoisomerase 6 subunit B, partial
(11%)
Length = 518
Score = 27.7 bits (60), Expect = 2.6
Identities = 12/39 (30%), Positives = 22/39 (55%)
Frame = -2
Query: 28 RTARDVLIFLQGWVTKFQKYQNSDFFITGESYAVWNLPL 66
RT ++L+ L ++T + N+ FITG+ +W + L
Sbjct: 508 RTHSNILLILLAFLTNKKNSYNNRSFITGDINLIWKINL 392
>AV426950
Length = 413
Score = 27.3 bits (59), Expect = 3.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 134 TFASHLNFSWFMRLQWKLERREMYVWKVKPPHT 166
T SH N SW ++ K ++ W V+PP T
Sbjct: 254 TRLSHTNVSWRVQPSLK*AAQKQIAWVVRPPST 156
>TC14106 similar to UP|Q42966 (Q42966) Nitrilase , partial (87%)
Length = 1286
Score = 27.3 bits (59), Expect = 3.4
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +3
Query: 193 SSTTGRILRIRLYLYWGDLLGPGYELWC 220
SSTT +L IRL W LLG G+ L C
Sbjct: 87 SSTTLPLLSIRLRDCWLKLLGMGHSLLC 170
>TC8589 UP|Q40210 (Q40210) RAB5B, complete
Length = 1241
Score = 27.3 bits (59), Expect = 3.4
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = -1
Query: 149 WKLERREMYVW 159
WKLER EMYVW
Sbjct: 80 WKLERVEMYVW 48
>TC14902
Length = 708
Score = 26.9 bits (58), Expect = 4.4
Identities = 18/44 (40%), Positives = 22/44 (49%), Gaps = 2/44 (4%)
Frame = -3
Query: 12 FSYSANTSDYFMVTDERT--ARDVLIFLQGWVTKFQKYQNSDFF 53
F Y+ + S YF DER A + L L W + K Q SDFF
Sbjct: 679 FQYNKSNSWYFSFLDERATGASETLQKLITWYSSLNKNQ-SDFF 551
>TC7983 weakly similar to GB|AAM19921.1|20453365|AY097405 AT3g23600/MDB19_9
{Arabidopsis thaliana;}, partial (94%)
Length = 1088
Score = 26.9 bits (58), Expect = 4.4
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = +3
Query: 243 RISVWTLVTTTGPGLMDHRLLDGLKLMVTSS 273
R+S WTL T TGP + +++D K++ S
Sbjct: 321 RVSPWTLGTLTGPFPLG*KIMDRTKVLKIQS 413
>TC13554
Length = 441
Score = 26.6 bits (57), Expect = 5.8
Identities = 13/41 (31%), Positives = 19/41 (45%)
Frame = -3
Query: 207 YWGDLLGPGYELWCIVETRIRSFH*LGQSLC*RGWPRISVW 247
YWG W I +IR+F + + G+PR+S W
Sbjct: 223 YWG--------AWLITMVQIRTFSLIRELQMSEGFPRLSTW 125
>TC17920
Length = 415
Score = 26.2 bits (56), Expect = 7.6
Identities = 9/20 (45%), Positives = 16/20 (80%)
Frame = +2
Query: 107 LVPFVIECFISLLLRLVHLL 126
L+P+VI+C L+LR++ L+
Sbjct: 215 LIPYVIKCICILMLRVIRLV 274
>AV428764
Length = 212
Score = 25.8 bits (55), Expect = 9.9
Identities = 13/34 (38%), Positives = 17/34 (49%), Gaps = 8/34 (23%)
Frame = +1
Query: 64 LPLITIPGLNFYGPMD*YPW--------KHTGCC 89
LPL T+P +GP+ YP +H GCC
Sbjct: 109 LPLTTVPNPRHHGPL--YPHDRRCGGCRRHDGCC 204
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.340 0.150 0.525
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,754,688
Number of Sequences: 28460
Number of extensions: 108698
Number of successful extensions: 790
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 784
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 787
length of query: 312
length of database: 4,897,600
effective HSP length: 90
effective length of query: 222
effective length of database: 2,336,200
effective search space: 518636400
effective search space used: 518636400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148469.4