BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148404.4 - phase: 0
(385 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP050885 34 0.047
TC9840 UP|R11D_LOTJA (Q40194) Ras-related protein Rab11D, complete 32 0.23
BI420443 31 0.30
TC11251 UP|R11E_LOTJA (Q40195) Ras-related protein Rab11E, complete 31 0.30
BI417933 31 0.40
AV421223 29 1.5
TC13465 similar to UP|O23110 (O23110) AP2 domain containing prot... 28 3.4
BI420710 27 5.7
TC17486 similar to UP|RU17_ARATH (Q42404) U1 small nuclear ribon... 23 6.1
TC10231 similar to UP|O23110 (O23110) AP2 domain containing prot... 27 7.5
TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , com... 27 7.5
TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fra... 27 7.5
TC8890 26 9.8
>BP050885
Length = 559
Score = 33.9 bits (76), Expect = 0.047
Identities = 25/78 (32%), Positives = 35/78 (44%)
Frame = -1
Query: 22 DNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGWQRFVKDHSLKENDFLVFKYNGE 81
DNL K+ +TL G + AR + + GW+ F H L + D L+F+
Sbjct: 511 DNLPKQ-DEMITLIDEDGDEYPTVYLARKKGL--SGGWRGFAIAHDLADGDALIFQLTNR 341
Query: 82 SLFEVLIFHGDSFCEKAA 99
S F+V I DS E A
Sbjct: 340 STFKVYIIRVDSPSEDTA 287
Score = 32.7 bits (73), Expect = 0.10
Identities = 29/87 (33%), Positives = 37/87 (42%), Gaps = 1/87 (1%)
Frame = -1
Query: 171 LALPKTFS-DNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFLNGWQRFVNDHSLKDND 229
L LP F DNL K+ E +TL G + + R + GW+ F H L D D
Sbjct: 538 LGLPCHFCIDNLPKQ-DEMITLIDEDGDEYPTVYLARKKGLS--GGWRGFAIAHDLADGD 368
Query: 230 FLVFNYNGESHFDVLIFDGESFCEKEA 256
L+F S F V I +S E A
Sbjct: 367 ALIFQLTNRSTFKVYIIRVDSPSEDTA 287
>TC9840 UP|R11D_LOTJA (Q40194) Ras-related protein Rab11D, complete
Length = 1302
Score = 31.6 bits (70), Expect = 0.23
Identities = 31/118 (26%), Positives = 47/118 (39%), Gaps = 15/118 (12%)
Frame = +2
Query: 204 ITRDDTVYFLNGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIF----DGESFCEKEASYF 259
+TR T W + + DH+ D + +V +S L+ DG+SF E+E+ YF
Sbjct: 530 VTRRATFENAARWLKELRDHT--DPNIVVMLIGNKSDLRHLVAVPTEDGKSFAERESLYF 703
Query: 260 VGKCSHTQTELGGSKANETNNSIEEVNT-----------ASSDGGVECGLYEKFQDLN 306
+ E +A N+ EV T + D G GL K Q +N
Sbjct: 704 M--------ETSALEATNVENAFTEVLTQIYRIVSKRAVEAGDSGSSSGLPSKGQTIN 853
>BI420443
Length = 493
Score = 31.2 bits (69), Expect = 0.30
Identities = 26/85 (30%), Positives = 43/85 (50%), Gaps = 3/85 (3%)
Frame = +3
Query: 168 KQQLALPKTFSDNLNKKLP-ENVTLKGPGGVAWKIGLITRDDT-VYFLNGWQRFVNDHSL 225
+ + LP+ F D+ + L E L P G + L+TR T V F +QR + H+L
Sbjct: 84 RNSMDLPRQFIDSHSHVLENEMAVLFDPVGNVFHC-LVTRCGTGVQFEEVFQRICHKHNL 260
Query: 226 KDNDFLVFNYNGESHFDVLIF-DGE 249
+ + + ++H+D+ IF DGE
Sbjct: 261 EGTSIIHLCHIHDNHYDIRIFGDGE 335
>TC11251 UP|R11E_LOTJA (Q40195) Ras-related protein Rab11E, complete
Length = 950
Score = 31.2 bits (69), Expect = 0.30
Identities = 24/88 (27%), Positives = 38/88 (42%), Gaps = 4/88 (4%)
Frame = +2
Query: 204 ITRDDTVYFLNGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIF----DGESFCEKEASYF 259
+TR T W + + DH+ D + +V +S L+ DG++F EKE+ YF
Sbjct: 344 VTRSTTFETAGRWLKELRDHT--DPNIVVMLIGNKSDLRHLVTVSTEDGKAFAEKESLYF 517
Query: 260 VGKCSHTQTELGGSKANETNNSIEEVNT 287
+ E +A N+ EV T
Sbjct: 518 M--------ETSALEATNVENAFSEVLT 577
>BI417933
Length = 568
Score = 30.8 bits (68), Expect = 0.40
Identities = 25/111 (22%), Positives = 46/111 (40%)
Frame = +2
Query: 171 LALPKTFSDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFLNGWQRFVNDHSLKDNDF 230
+ +PK F + + ++ V L+ P G + + G ++ H ++
Sbjct: 245 IVVPKRFHEPVGYEINNVVQLQDPVGNTFNLSYYFNQGDHRIGAGLFNILSVHEIQHTVM 424
Query: 231 LVFNYNGESHFDVLIFDGESFCEKEASYFVGKCSHTQTELGGSKANETNNS 281
+ F Y G++ F++ IFD S +E Y V Q L A + +NS
Sbjct: 425 VHFLYRGQARFNIHIFDPFS---REIRYNVAPQLPPQINLIIDDAEDNDNS 568
>AV421223
Length = 414
Score = 28.9 bits (63), Expect = 1.5
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = +2
Query: 129 STPSNGSVECSLPEKSWEEDIYWTHFQFIHF 159
S P+NG VE E S E++YW F + F
Sbjct: 140 SGPNNGDVETKEQEVSILENVYWKEFGLLAF 232
>TC13465 similar to UP|O23110 (O23110) AP2 domain containing protein
RAP2.8 (Fragment), partial (18%)
Length = 609
Score = 27.7 bits (60), Expect = 3.4
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +1
Query: 56 TNGWQRFVKDHSLKENDFLVF 76
T GW RFVK+ LK D + F
Sbjct: 43 TKGWSRFVKEKCLKAGDSVCF 105
>BI420710
Length = 629
Score = 26.9 bits (58), Expect = 5.7
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = +1
Query: 89 FHGDSFCEKAASYFVGKCGQAHTEQVCNKGKSSNNSVEEVSTPSNGSVECSLPEKSWEED 148
FHGD + + G A T + K SSN S++ N ++ KS ED
Sbjct: 286 FHGDDYLNPEDN---DHFGYARTNRQYRKASSSNLSIQSDDHNDNLNLNDWTTGKSAGED 456
Query: 149 IYWT 152
I++T
Sbjct: 457 IFFT 468
>TC17486 similar to UP|RU17_ARATH (Q42404) U1 small nuclear
ribonucleoprotein 70 kDa (U1 snRNP 70 kDa) (snRNP70)
(U1-70K), partial (39%)
Length = 595
Score = 23.5 bits (49), Expect(2) = 6.1
Identities = 8/22 (36%), Positives = 13/22 (58%)
Frame = +1
Query: 196 GVAWKIGLITRDDTVYFLNGWQ 217
G+ W I L+ R++ V GW+
Sbjct: 232 GLHWSISLLRRNENVLPYQGWR 297
Score = 21.6 bits (44), Expect(2) = 6.1
Identities = 9/29 (31%), Positives = 13/29 (44%), Gaps = 2/29 (6%)
Frame = +2
Query: 131 PSNGSVECSLP--EKSWEEDIYWTHFQFI 157
P GS P + W E YW+H + +
Sbjct: 128 PCQGSEPRQRPPAQADWTESSYWSHCESV 214
>TC10231 similar to UP|O23110 (O23110) AP2 domain containing protein RAP2.8
(Fragment), partial (22%)
Length = 707
Score = 26.6 bits (57), Expect = 7.5
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +1
Query: 56 TNGWQRFVKDHSLKENDFLVF 76
T GW RFVK+ +L+ D + F
Sbjct: 133 TKGWSRFVKEKNLRAGDAVRF 195
>TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , complete
Length = 3376
Score = 26.6 bits (57), Expect = 7.5
Identities = 18/59 (30%), Positives = 28/59 (46%), Gaps = 3/59 (5%)
Frame = +3
Query: 116 NKGKSSNNSVEEVSTPSNGSVECSLPEKSWEEDIYWTHFQFI---HFTQLLPEDFKQQL 171
N K S+ + VS +NG K + +++WTHF + H T L P ++ QL
Sbjct: 1524 NGRKKSDRNGCWVS*AANGHCSDLTSPKLRKSEMFWTHFMSLPNFHRTTLEPISYQWQL 1700
>TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fragment),
partial (48%)
Length = 679
Score = 26.6 bits (57), Expect = 7.5
Identities = 17/51 (33%), Positives = 24/51 (46%), Gaps = 4/51 (7%)
Frame = -2
Query: 109 AHTEQVCNKGKSSNNSVEEVSTPSNGSVEC----SLPEKSWEEDIYWTHFQ 155
+HTE + +K +N V+ +S S E S P K W I+W H Q
Sbjct: 411 SHTEILHHK---PDNQVDRMSPSSQNCTEALQSRSTPSKDWRNHIWW-HLQ 271
>TC8890
Length = 410
Score = 26.2 bits (56), Expect = 9.8
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +3
Query: 90 HGDSFCEKAASYFVGKCGQAHT 111
HG S C K AS F +C + HT
Sbjct: 201 HG*SLCLKRASLFNARCTRIHT 266
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.133 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,298,129
Number of Sequences: 28460
Number of extensions: 103167
Number of successful extensions: 521
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 521
length of query: 385
length of database: 4,897,600
effective HSP length: 92
effective length of query: 293
effective length of database: 2,279,280
effective search space: 667829040
effective search space used: 667829040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148404.4