BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148349.9 - phase: 0
(149 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9783 similar to UP|R191_ARATH (Q9SGA6) 40S ribosomal protein S... 271 4e-74
TC9478 similar to UP|R193_ARATH (Q9FNP8) 40S ribosomal protein S... 260 6e-71
TC19069 similar to UP|R191_ARATH (Q9SGA6) 40S ribosomal protein ... 216 2e-57
AU088865 150 9e-38
AV767447 37 0.001
AV765124 28 0.66
BP061925 27 1.5
TC10372 27 1.5
BP042660 26 3.3
AV781307 26 3.3
TC18660 25 4.3
TC20255 weakly similar to UP|Q8L9U0 (Q8L9U0) Coclaurine N-methyl... 25 4.3
TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase (AT3G267... 25 5.6
TC8694 similar to UP|IF38_MEDTR (Q9XHM1) Eukaryotic translation ... 25 7.3
BP075774 25 7.3
AV773361 24 9.5
TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 ho... 24 9.5
>TC9783 similar to UP|R191_ARATH (Q9SGA6) 40S ribosomal protein S19-1,
complete
Length = 772
Score = 271 bits (692), Expect = 4e-74
Identities = 130/144 (90%), Positives = 141/144 (97%)
Frame = +2
Query: 1 MASSKTVKDVSPREFVNAYSAHLKRSGKLELPEWTDLVKTAKFKELAPYDPDWYYVRAAS 60
MAS+KTVKDVSP EFV AYSAHLKRSGK+ELPEWTD+VKTA+FKELAPYDPDWYYVRAAS
Sbjct: 86 MASAKTVKDVSPHEFVKAYSAHLKRSGKMELPEWTDIVKTARFKELAPYDPDWYYVRAAS 265
Query: 61 MARKIYVRGGLGVGSFQRIYGGSQRNGSRPPHFCKSSGAIARHILQQLENMNLIEMDTKG 120
MARKIY+RGGLGVG+FQRIYGGSQRNGSRPPHFCKSSGAIAR+ILQQL+ MN+IEMDTKG
Sbjct: 266 MARKIYLRGGLGVGAFQRIYGGSQRNGSRPPHFCKSSGAIARNILQQLQEMNIIEMDTKG 445
Query: 121 GRKITSSGRRDLDQVAGRILVAP* 144
GR+ITSSGRRDLDQVAGRI+VAP*
Sbjct: 446 GRRITSSGRRDLDQVAGRIVVAP* 517
>TC9478 similar to UP|R193_ARATH (Q9FNP8) 40S ribosomal protein S19-3,
partial (97%)
Length = 706
Score = 260 bits (665), Expect = 6e-71
Identities = 124/150 (82%), Positives = 143/150 (94%), Gaps = 1/150 (0%)
Frame = +1
Query: 1 MASSKTVKDVSPREFVNAYSAHLKRSGKLELPEWTDLVKTAKFKELAPYDPDWYYVRAAS 60
M++++TVKDVSP EFV AY+AHLKRSGK+ELPEWTD+VKTA+FKELAPYDPDWYY+RAAS
Sbjct: 61 MSTARTVKDVSPHEFVKAYAAHLKRSGKMELPEWTDIVKTARFKELAPYDPDWYYIRAAS 240
Query: 61 MARKIYVRGGLGVGSFQRIYGGSQRNGSRPPHFCKSSGAIARHILQQLENMNLIEMDTKG 120
MARKIY+RGGLGVG+FQRIYGGSQRNGSRPPHFCKSSG+IARHILQQL+ M++IE+D KG
Sbjct: 241 MARKIYLRGGLGVGAFQRIYGGSQRNGSRPPHFCKSSGSIARHILQQLQTMHIIELDNKG 420
Query: 121 GRKITSSGRRDLDQVAGRI-LVAP*FVSKS 149
GRKITSSGRRDLDQVAGRI +VAP* V+ +
Sbjct: 421 GRKITSSGRRDLDQVAGRIVVVAP*SVTST 510
>TC19069 similar to UP|R191_ARATH (Q9SGA6) 40S ribosomal protein S19-1,
partial (78%)
Length = 360
Score = 216 bits (549), Expect = 2e-57
Identities = 100/112 (89%), Positives = 110/112 (97%)
Frame = +2
Query: 1 MASSKTVKDVSPREFVNAYSAHLKRSGKLELPEWTDLVKTAKFKELAPYDPDWYYVRAAS 60
MA++KTVKDVSP EFV AYS+HLKRSGK+ELPEWTD+VKTAKFKELAPYDPDWYYVRAAS
Sbjct: 23 MATAKTVKDVSPHEFVKAYSSHLKRSGKMELPEWTDIVKTAKFKELAPYDPDWYYVRAAS 202
Query: 61 MARKIYVRGGLGVGSFQRIYGGSQRNGSRPPHFCKSSGAIARHILQQLENMN 112
MARKIY+RGGLGVG+F+RIYGGS+RNGSRPPHFCKSSGAIARHILQQL+NMN
Sbjct: 203 MARKIYLRGGLGVGAFRRIYGGSKRNGSRPPHFCKSSGAIARHILQQLQNMN 358
>AU088865
Length = 443
Score = 150 bits (379), Expect = 9e-38
Identities = 80/129 (62%), Positives = 86/129 (66%), Gaps = 4/129 (3%)
Frame = +3
Query: 1 MASSKTVKDVSPREFVNAYSAHLKRSGKLELPEWTDLVKTAKFKELAPYDPDWYYVRAAS 60
MAS+KTVKDVSP EFV AYSAHLKRSGK+ELPEWTD+VKTA+FKELAPYDPDWYYV AS
Sbjct: 72 MASAKTVKDVSPHEFVKAYSAHLKRSGKMELPEWTDIVKTARFKELAPYDPDWYYVXXAS 251
Query: 61 MARKIYVRGGLGVGSFQRIYGGSQRNGSRP----PHFCKSSGAIARHILQQLENMNLIEM 116
MARKIY+ G YGG NG+RP H C S N EM
Sbjct: 252 MARKIYLXRGSWCWCISXDYGGXXXNGTRPLXLQNHRCHCS*X-----SPPFAGNNFFEM 416
Query: 117 DTKGGRKIT 125
D KGGRKIT
Sbjct: 417 DXKGGRKIT 443
>AV767447
Length = 259
Score = 37.0 bits (84), Expect = 0.001
Identities = 19/23 (82%), Positives = 22/23 (95%), Gaps = 1/23 (4%)
Frame = -1
Query: 124 ITSSGRRDLDQVAGR-ILVAP*F 145
ITSSGRRDLDQVAGR ++VAP*+
Sbjct: 259 ITSSGRRDLDQVAGRTVVVAP*Y 191
>AV765124
Length = 532
Score = 28.1 bits (61), Expect = 0.66
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 5/34 (14%)
Frame = +2
Query: 81 GGSQRNG-----SRPPHFCKSSGAIARHILQQLE 109
GGSQ G SRPPHF ++ H L Q++
Sbjct: 329 GGSQGTGTFHSVSRPPHFRDKKNEVSNH*LNQIK 430
>BP061925
Length = 356
Score = 26.9 bits (58), Expect = 1.5
Identities = 18/33 (54%), Positives = 21/33 (63%), Gaps = 5/33 (15%)
Frame = +1
Query: 83 SQRNGS--RPPHFCKSSGAIA---RHILQQLEN 110
+Q NGS RP HF KSS AIA H L+QL +
Sbjct: 214 TQLNGSYKRPLHFRKSSSAIASLTTHSLKQLRS 312
>TC10372
Length = 612
Score = 26.9 bits (58), Expect = 1.5
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = +3
Query: 78 RIYGGSQRNGSRPPHFCKSSGAIARHI 104
R+YG +Q NG +PPH A H+
Sbjct: 330 RVYGIAQYNGGQPPHNLDMGVAAPAHL 410
>BP042660
Length = 474
Score = 25.8 bits (55), Expect = 3.3
Identities = 11/38 (28%), Positives = 25/38 (64%), Gaps = 1/38 (2%)
Frame = -2
Query: 101 ARHILQQLENMNLIEMDT-KGGRKITSSGRRDLDQVAG 137
++ + ++E+ NL+E+DT K G++ T + D+ ++ G
Sbjct: 392 SKSLTSRIESSNLVELDTCKLGKEATQNNVTDMAKLCG 279
>AV781307
Length = 710
Score = 25.8 bits (55), Expect = 3.3
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +3
Query: 33 EWTDLVKTAKFKELAPYDPDW 53
+W D V+ A+ K P +P+W
Sbjct: 531 KWCDTVRIARHKRSRPTEPEW 593
>TC18660
Length = 893
Score = 25.4 bits (54), Expect = 4.3
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +2
Query: 75 SFQRIYGGSQRNGSRPPHFCKS-SGAIARHI 104
SF I GG+ G+R PH S SG++A +I
Sbjct: 401 SFGEIRGGAAMIGNRNPHMGPSQSGSVAHNI 493
>TC20255 weakly similar to UP|Q8L9U0 (Q8L9U0) Coclaurine
N-methyltransferase, partial (38%)
Length = 419
Score = 25.4 bits (54), Expect = 4.3
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -3
Query: 93 FCKSSGAIARHILQQLENMNLIEMDTKGGRKITSS 127
FC+ S RHILQ + +N + GR +T +
Sbjct: 222 FCRISADGNRHILQSMHKVNKVRELNLRGRFVTGA 118
>TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase
(AT3G26720/MLJ15_12), partial (14%)
Length = 547
Score = 25.0 bits (53), Expect = 5.6
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +1
Query: 43 FKELAPYDPDWYYVRAASMARKIYVRGGLGVGSFQRIYGGSQRNGSRPPHF 93
+ E+A + W R S ARKI V+ + G + I GG + PH+
Sbjct: 322 YVEMAFFQRWW---RQQSKARKIKVKELVNSGQLEFINGGMCMHDEATPHY 465
>TC8694 similar to UP|IF38_MEDTR (Q9XHM1) Eukaryotic translation initiation
factor 3 subunit 8 (eIF3 p110) (eIF3c), partial (23%)
Length = 986
Score = 24.6 bits (52), Expect = 7.3
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +2
Query: 68 RGGLGVGSFQRIYGGSQR 85
RGG G G +Q YGGS R
Sbjct: 638 RGGGGGGGYQSQYGGSGR 691
>BP075774
Length = 391
Score = 24.6 bits (52), Expect = 7.3
Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 8/45 (17%)
Frame = -2
Query: 69 GGLGVGSFQRIY--------GGSQRNGSRPPHFCKSSGAIARHIL 105
GG G G +Q Y GGS G+ PH+ S G +R ++
Sbjct: 315 GGGGGGGYQSPYRGSGRAHPGGSALQGTPCPHWIFSPGLASRELV 181
>AV773361
Length = 555
Score = 24.3 bits (51), Expect = 9.5
Identities = 15/46 (32%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = +1
Query: 91 PHFCKSSGAIAR-HILQQLENMNLIEMDTKGGRKITSSGRRDLDQV 135
P F S+ A+ H +Q+ M L+ + + R+ TSS LDQ+
Sbjct: 397 PLFLPSTPAVVY*HYREQVLQMQLVSVQFQEKRQQTSSSPLHLDQI 534
>TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 homolog
(At3g57490), partial (91%)
Length = 1076
Score = 24.3 bits (51), Expect = 9.5
Identities = 21/68 (30%), Positives = 29/68 (41%)
Frame = +2
Query: 68 RGGLGVGSFQRIYGGSQRNGSRPPHFCKSSGAIARHILQQLENMNLIEMDTKGGRKITSS 127
RGG G F R +GG R G R G R ++ E + + TK GR +
Sbjct: 89 RGGGDRGGFGRGFGGRGRGGDR------GRGGRRRGAGRRDEEEKWVPV-TKLGRLVRDG 247
Query: 128 GRRDLDQV 135
R L+Q+
Sbjct: 248 KIRSLEQI 271
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,158,828
Number of Sequences: 28460
Number of extensions: 24700
Number of successful extensions: 137
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of query: 149
length of database: 4,897,600
effective HSP length: 82
effective length of query: 67
effective length of database: 2,563,880
effective search space: 171779960
effective search space used: 171779960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148349.9