BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148349.4 - phase: 0
(151 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP036178 43 2e-05
TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutat... 41 8e-05
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 39 3e-04
BP049972 39 5e-04
TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17... 30 0.18
TC11204 similar to GB|AAO64909.1|29029060|BT005974 At1g77410 {Ar... 28 0.52
TC13987 similar to UP|Q45307 (Q45307) 5A2 divIB (Fragment), part... 25 0.53
BI418474 27 1.5
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 27 2.0
TC7995 similar to PIR|S63686|S63686 sterol 24-C-methyltransferas... 27 2.0
TC9391 homologue to UP|MSK1_MEDSA (P51137) Glycogen synthase kin... 26 2.6
TC19465 similar to AAR92318 (AAR92318) At5g10310, partial (16%) 26 2.6
TC10612 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protei... 26 2.6
BP045380 26 2.6
TC17929 26 2.6
TC14160 homologue to GB|BAB90396.1|20161472|AP003570 ADP-ribosyl... 26 2.6
AV425893 26 3.4
AU251926 26 3.4
BP078534 26 3.4
NP645867 proliferating floral organs protein [Lotus japonicus] 25 4.4
>BP036178
Length = 561
Score = 43.1 bits (100), Expect = 2e-05
Identities = 27/89 (30%), Positives = 35/89 (38%), Gaps = 11/89 (12%)
Frame = -2
Query: 63 YRATYNYFIQPVNSQIYWEPTPYEKSA-----------PPKVRRAAGRPKKNRRKDGNKE 111
YR Y+ I P+ + W PPK RR GRPKK + N
Sbjct: 560 YRMAYSQIIIPIPDRSQWREHGEGAEGVGGARVDIVIRPPKTRRPPGRPKKKVLRVEN-- 387
Query: 112 PIGRSQMKRTYNDTQCGRCGLIGHNLRGC 140
KR QCGRC ++GH+ + C
Sbjct: 386 ------FKRPKRIVQCGRCHMLGHSQKKC 318
>TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (4%)
Length = 513
Score = 41.2 bits (95), Expect = 8e-05
Identities = 27/94 (28%), Positives = 40/94 (41%), Gaps = 3/94 (3%)
Frame = +3
Query: 52 EHCHAWLTLGSYRATYNYFIQPVNSQIYWE---PTPYEKSAPPKVRRAAGRPKKNRRKDG 108
++C + T SYR TY+ + P+ E P PP RR GRP
Sbjct: 12 DYCSRYCTTESYRLTYSERVNPIPIMAVPESKDSQPVVTVTPPFTRRPPGRP-------A 170
Query: 109 NKEPIGRSQMKRTYNDTQCGRCGLIGHNLRGCNK 142
K + ++ +KR + C RC +GHN C +
Sbjct: 171 TKRYVSQNIVKR---ELHCSRCKGLGHNKSTCKE 263
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 39.3 bits (90), Expect = 3e-04
Identities = 28/92 (30%), Positives = 42/92 (45%), Gaps = 10/92 (10%)
Frame = +3
Query: 59 TLGSYRATYNYFIQPVNSQIYW-EPTPYEKSA---------PPKVRRAAGRPKKNRRKDG 108
T+ +YR TY+ I P+ + W E + +++A PPK R GRP+K R +
Sbjct: 15 TVATYRKTYSQTIHPIPDKSLWKELSEGDENAAKADQVLINPPKSLRPPGRPRKKRVRAE 194
Query: 109 NKEPIGRSQMKRTYNDTQCGRCGLIGHNLRGC 140
+ R ++KR C RC GH C
Sbjct: 195 D-----RGRVKRV---VHCSRCNQTGHFRTTC 266
>BP049972
Length = 496
Score = 38.5 bits (88), Expect = 5e-04
Identities = 27/101 (26%), Positives = 39/101 (37%), Gaps = 19/101 (18%)
Frame = -1
Query: 59 TLGSYRATYNYFIQPVNSQIYWEPTPYEKS-------------------APPKVRRAAGR 99
T+ +YR TY+ I P+ + +W E + PPK R GR
Sbjct: 472 TVATYRKTYSQTIHPIPDRSFWNELSIEDANANKAVEDANASQSIEIVINPPKSLRPPGR 293
Query: 100 PKKNRRKDGNKEPIGRSQMKRTYNDTQCGRCGLIGHNLRGC 140
P+K R + + R ++KR C RC GH C
Sbjct: 292 PRKKRVRAED-----RGRVKRV---VHCSRCNQTGHFRTTC 194
>TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;}, partial (6%)
Length = 527
Score = 30.0 bits (66), Expect = 0.18
Identities = 16/51 (31%), Positives = 23/51 (44%)
Frame = +3
Query: 90 PPKVRRAAGRPKKNRRKDGNKEPIGRSQMKRTYNDTQCGRCGLIGHNLRGC 140
PP +R GRPK +P+ M + QC +C +GHN + C
Sbjct: 42 PPPTKRPPGRPKM--------KPVESIDMIK--RQLQCSKCKELGHNKKTC 164
>TC11204 similar to GB|AAO64909.1|29029060|BT005974 At1g77410 {Arabidopsis
thaliana;}, partial (13%)
Length = 568
Score = 28.5 bits (62), Expect = 0.52
Identities = 14/37 (37%), Positives = 21/37 (55%), Gaps = 6/37 (16%)
Frame = +1
Query: 57 WLTLGSYRAT-YNYFIQ-----PVNSQIYWEPTPYEK 87
W LG + T +N+F + P + +IYWEPT Y +
Sbjct: 283 WPILGIFPHTRWNFFTELVSHTPFHPKIYWEPTSYTR 393
>TC13987 similar to UP|Q45307 (Q45307) 5A2 divIB (Fragment), partial (8%)
Length = 491
Score = 25.0 bits (53), Expect(2) = 0.53
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = +2
Query: 33 SHNWTPLWSGDTPMQRYQIEHCHAW 57
+H W+ WS P ++ H HAW
Sbjct: 83 AHAWSYYWSNLMPTHVRRLIHEHAW 157
Score = 21.9 bits (45), Expect(2) = 0.53
Identities = 8/28 (28%), Positives = 13/28 (45%)
Frame = +2
Query: 53 HCHAWLTLGSYRATYNYFIQPVNSQIYW 80
H HAW+T G+ + F + +W
Sbjct: 227 HAHAWMTAGNSISDLGTF*TKLKGLGFW 310
>BI418474
Length = 479
Score = 26.9 bits (58), Expect = 1.5
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +3
Query: 18 LCPWQQSRLEKEKNASHNWTPLWSGDTPMQRYQIEHCHAW 57
L P Q +L+KE N + + G P RY++ H W
Sbjct: 192 LTPLQVEKLKKEANGN-----VLEGSRPYSRYEVIGAHIW 296
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 26.6 bits (57), Expect = 2.0
Identities = 17/48 (35%), Positives = 19/48 (39%)
Frame = +2
Query: 93 VRRAAGRPKKNRRKDGNKEPIGRSQMKRTYNDTQCGRCGLIGHNLRGC 140
V A G P+ NR G P G + C CGL GH R C
Sbjct: 296 VEFAKGGPRGNREYLGRGPPPGSGR---------CFNCGLDGHWARDC 412
>TC7995 similar to PIR|S63686|S63686 sterol 24-C-methyltransferase -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (98%)
Length = 1524
Score = 26.6 bits (57), Expect = 2.0
Identities = 22/73 (30%), Positives = 29/73 (39%)
Frame = -2
Query: 19 CPWQQSRLEKEKNASHNWTPLWSGDTPMQRYQIEHCHAWLTLGSYRATYNYFIQPVNSQI 78
C S+ ++ ASH P S PM+R Q C + T +T PV S
Sbjct: 1163 CHLLWSKSQRS*TASHEHPPSPSARFPMRREQSPQCGSSSTRSYPSSTGPTTEAPVVSP- 987
Query: 79 YWEPTPYEKSAPP 91
+PTP PP
Sbjct: 986 --DPTPSPPRTPP 954
>TC9391 homologue to UP|MSK1_MEDSA (P51137) Glycogen synthase kinase-3
homolog MsK-1 , partial (48%)
Length = 841
Score = 26.2 bits (56), Expect = 2.6
Identities = 9/40 (22%), Positives = 18/40 (44%)
Frame = +3
Query: 19 CPWQQSRLEKEKNASHNWTPLWSGDTPMQRYQIEHCHAWL 58
C W + + + ++W WS + + Y +C AW+
Sbjct: 351 C*W*RCNGDWTYHCHYHWGQKWSAEADYKLYGRAYCRAWI 470
>TC19465 similar to AAR92318 (AAR92318) At5g10310, partial (16%)
Length = 547
Score = 26.2 bits (56), Expect = 2.6
Identities = 10/23 (43%), Positives = 15/23 (64%), Gaps = 2/23 (8%)
Frame = -3
Query: 43 DTPMQRYQIE--HCHAWLTLGSY 63
D P+ Q+ HCHAW+TL ++
Sbjct: 188 DNPVMARQVGNLHCHAWMTLVAF 120
>TC10612 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protein L10 (QM
protein homolog), partial (68%)
Length = 474
Score = 26.2 bits (56), Expect = 2.6
Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Frame = -2
Query: 80 WEPTPYEKSAPPKV---RRAAGRPKKNRRKDGNKEPIGRSQMKRTYNDTQCGRCGLIG 134
W T +SAPP+V R + ++ R+ G + + S +R + GR G G
Sbjct: 257 WPCTCCRQSAPPRVLHWRHSPSPMRRGGRRRGTRLRLSSSSRRRRFGSLGPGRRGRSG 84
>BP045380
Length = 558
Score = 26.2 bits (56), Expect = 2.6
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = -1
Query: 82 PTPYEKSAPPKVRRAAGRPKKNRRKDGNKEPIGRSQMKRT 121
PTP + + PP+ R + P K +++ P+ +++R+
Sbjct: 531 PTPMKPNPPPQPPRRSQEPPKRKQEQQVLRPVNLLRLERS 412
>TC17929
Length = 791
Score = 26.2 bits (56), Expect = 2.6
Identities = 9/17 (52%), Positives = 11/17 (63%)
Frame = +2
Query: 127 CGRCGLIGHNLRGCNKQ 143
C RCG GH +R C K+
Sbjct: 62 CYRCGESGHKMRNCPKE 112
>TC14160 homologue to GB|BAB90396.1|20161472|AP003570 ADP-ribosylation
factor {Oryza sativa (japonica cultivar-group);},
partial (48%)
Length = 1105
Score = 26.2 bits (56), Expect = 2.6
Identities = 17/59 (28%), Positives = 24/59 (39%), Gaps = 19/59 (32%)
Frame = +2
Query: 105 RKDGNKEPIG-----------------RSQMKRTYNDTQCGRCGLIGHNLR--GCNKQG 144
R++GN P G R + +Y+ QCG CG+ H L GC + G
Sbjct: 245 REEGNANPHGWSRCGW*DHHPLQTQAWRDRHHNSYHWVQCGDCGIQEH*LHCLGCRRPG 421
>AV425893
Length = 414
Score = 25.8 bits (55), Expect = 3.4
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -2
Query: 94 RRAAGRPKKNRRKDGNKEPIGRSQMKRTYNDTQCGRCGLI 133
+R+ + K G+ +P GR + +R ND Q G CGL+
Sbjct: 155 KRSQAQDKGKPLPVGDNKP-GRRRDRRGGNDVQDGGCGLV 39
>AU251926
Length = 335
Score = 25.8 bits (55), Expect = 3.4
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -3
Query: 26 LEKEKNASHNWTPLWSGDTPMQRYQIEHCHAWLTL 60
L K+ NW P S T ++R+ IE +W+ L
Sbjct: 333 LPKDAGRDGNWFPRRSR*TRLRRFPIESGSSWILL 229
>BP078534
Length = 410
Score = 25.8 bits (55), Expect = 3.4
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +3
Query: 24 SRLEKEKNASHNWTPLWSGDTPMQRYQIEH 53
+ LE N SHN T LW + P + H
Sbjct: 318 THLELSYNCSHNLTTLWCPEAPSDAL*LAH 407
>NP645867 proliferating floral organs protein [Lotus japonicus]
Length = 1350
Score = 25.4 bits (54), Expect = 4.4
Identities = 11/17 (64%), Positives = 12/17 (69%), Gaps = 3/17 (17%)
Frame = +2
Query: 127 CGRCGLIGH---NLRGC 140
CGRC L+G NLRGC
Sbjct: 1025 CGRCRLVGPCGWNLRGC 1075
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,231,353
Number of Sequences: 28460
Number of extensions: 47975
Number of successful extensions: 340
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 329
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 334
length of query: 151
length of database: 4,897,600
effective HSP length: 82
effective length of query: 69
effective length of database: 2,563,880
effective search space: 176907720
effective search space used: 176907720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148349.4