BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148349.13 - phase: 0
(348 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10824 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransf... 231 1e-61
AV765294 198 1e-51
TC19305 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransf... 182 9e-47
TC19931 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransf... 139 7e-34
TC10362 similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-l... 133 4e-32
TC13838 weakly similar to UP|F3ST_FLABI (P52835) Flavonol 3-sulf... 118 1e-27
BP056308 28 1.7
TC12913 homologue to UP|Q84L76 (Q84L76) Expansin precursor, part... 28 1.7
TC9508 weakly similar to UP|Q7XQG9 (Q7XQG9) OJ000114_01.8 protei... 27 5.1
BP040968 27 5.1
BP044824 27 6.6
TC15913 26 8.7
>TC10824 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (39%)
Length = 604
Score = 231 bits (589), Expect = 1e-61
Identities = 106/149 (71%), Positives = 130/149 (87%)
Frame = +1
Query: 197 KSLTELTLEESFERYCKGICLFGPFWDNMLGYLKESIERPDRVLFLKYEDLKEDVNFHTK 256
+ L E TLEE+FERYCKG+ +GP WD+MLGYLKES+ RP++VLFLKYEDLKEDVNFH K
Sbjct: 13 EELPEFTLEEAFERYCKGVTEYGPTWDHMLGYLKESVARPNKVLFLKYEDLKEDVNFHVK 192
Query: 257 RIAEFVGIPFTQEEENNGVIENIIKLCSFESMKEIEGNQSGTISGDIEKEFYFRKGEIGD 316
RI+EF+G+PFTQEEE+ V+ENIIKLCSFE MK++E N+SGT+ + EK+++FRK EIGD
Sbjct: 193 RISEFLGVPFTQEEESGEVVENIIKLCSFEKMKDLEVNKSGTLGLNFEKKYFFRKAEIGD 372
Query: 317 WANYLSSSMVEKLSKVMEEKLNGSSLSFK 345
W NYLS SMVEKLSK+MEEKL+GS LSF+
Sbjct: 373 WVNYLSPSMVEKLSKIMEEKLSGSGLSFR 459
>AV765294
Length = 530
Score = 198 bits (504), Expect = 1e-51
Identities = 87/138 (63%), Positives = 115/138 (83%)
Frame = -3
Query: 211 YCKGICLFGPFWDNMLGYLKESIERPDRVLFLKYEDLKEDVNFHTKRIAEFVGIPFTQEE 270
+C G +GPFW+++LGY KESI RP++VLFLKYEDLK+DVNF+ KR+ EF+G PFT EE
Sbjct: 528 HCNGTTRYGPFWNHILGYWKESIARPEKVLFLKYEDLKDDVNFYVKRVGEFLGCPFTHEE 349
Query: 271 ENNGVIENIIKLCSFESMKEIEGNQSGTISGDIEKEFYFRKGEIGDWANYLSSSMVEKLS 330
E++GVIE+I++LCSFE MKE+E N+ GT + D+ + +FRKGE+GDW NYLS SMVEKLS
Sbjct: 348 ESSGVIEDIVQLCSFEKMKELEVNKCGTFTRDVGNKHFFRKGEVGDWVNYLSPSMVEKLS 169
Query: 331 KVMEEKLNGSSLSFKVCA 348
K++EEK GS LSF++C+
Sbjct: 168 KIIEEKFRGSGLSFRMCS 115
>TC19305 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (33%)
Length = 567
Score = 182 bits (461), Expect = 9e-47
Identities = 83/122 (68%), Positives = 105/122 (86%)
Frame = +3
Query: 226 LGYLKESIERPDRVLFLKYEDLKEDVNFHTKRIAEFVGIPFTQEEENNGVIENIIKLCSF 285
LGY KES+ RPD+VLFLKYEDLKEDV+FH +R+AEF+ FTQEE + GVIENIIKLCSF
Sbjct: 3 LGYWKESMARPDKVLFLKYEDLKEDVDFHVRRVAEFLDCGFTQEEVSGGVIENIIKLCSF 182
Query: 286 ESMKEIEGNQSGTISGDIEKEFYFRKGEIGDWANYLSSSMVEKLSKVMEEKLNGSSLSFK 345
E MK +E N+SGT+ ++E +++FRKG++GDW NYLS+SMVEKLSK++EEKL+G LSF+
Sbjct: 183 EKMKALEVNKSGTVGRNVENKYFFRKGQVGDWVNYLSTSMVEKLSKIIEEKLSGEGLSFR 362
Query: 346 VC 347
VC
Sbjct: 363 VC 368
>TC19931 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (29%)
Length = 539
Score = 139 bits (350), Expect = 7e-34
Identities = 69/133 (51%), Positives = 90/133 (66%)
Frame = +3
Query: 1 MASTDLTNMKEDQSRDGQETTSKVNKVSHDYNNLILSLPRENGCETQYMYFFHGFWCPST 60
MASTD T++ E+QSR+ + +S + LI +LPRE G +Y+Y F GFWC
Sbjct: 147 MASTDPTHVTENQSREEES-------LSRECKELIPTLPREKGWTVRYLYLFQGFWCNPH 305
Query: 61 LIQSVNSFQNNFHAKDSDIVVASIPKSGTTWLKGLAYAIVNRQHFTSLENNHPLLLFNPH 120
IQ + +FQ F AKDSD++VA+ PKSGTTWLK L +A+VNR +F S E NHPLL NPH
Sbjct: 306 RIQPIYTFQKQFQAKDSDVIVATYPKSGTTWLKALTFAVVNRHNFPSSE-NHPLLTSNPH 482
Query: 121 ELVPQFEVNLYGD 133
LVP FE ++YG+
Sbjct: 483 HLVPFFETSIYGE 521
>TC10362 similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like protein
(At5g07010), partial (23%)
Length = 425
Score = 133 bits (335), Expect = 4e-32
Identities = 61/120 (50%), Positives = 81/120 (66%)
Frame = +3
Query: 16 DGQETTSKVNKVSHDYNNLILSLPRENGCETQYMYFFHGFWCPSTLIQSVNSFQNNFHAK 75
+G E ++S + +LILSLPRE G T+Y+Y F GFWC IQ++++FQ +F AK
Sbjct: 51 NGDEGAIDQEELSEEMKDLILSLPRERGWRTKYLYLFQGFWCQPAEIQAISTFQKHFQAK 230
Query: 76 DSDIVVASIPKSGTTWLKGLAYAIVNRQHFTSLENNHPLLLFNPHELVPQFEVNLYGDKD 135
SD+ VA++PKSGTTWLK L +AIVNRQ + +HPLL NPH+LVP E +YG D
Sbjct: 231 HSDVFVATVPKSGTTWLKALTFAIVNRQKHLTFSKSHPLLNSNPHDLVPFIEYTVYGKHD 410
>TC13838 weakly similar to UP|F3ST_FLABI (P52835) Flavonol
3-sulfotransferase (F3-ST) , partial (13%)
Length = 484
Score = 118 bits (296), Expect = 1e-27
Identities = 59/91 (64%), Positives = 72/91 (78%)
Frame = +3
Query: 256 KRIAEFVGIPFTQEEENNGVIENIIKLCSFESMKEIEGNQSGTISGDIEKEFYFRKGEIG 315
K+IAEF+G F+ EEE+ GVIENII LCSFE MKE+E N+SGT + + + +F FRK EIG
Sbjct: 6 KKIAEFLGCSFSLEEESGGVIENIIDLCSFEKMKELEVNKSGTFARNFKNKFLFRKAEIG 185
Query: 316 DWANYLSSSMVEKLSKVMEEKLNGSSLSFKV 346
D+ N+LS MVEKLSKVMEEKL G LSF+V
Sbjct: 186 DFVNHLSPFMVEKLSKVMEEKLGGCGLSFRV 278
>BP056308
Length = 549
Score = 28.5 bits (62), Expect = 1.7
Identities = 17/57 (29%), Positives = 27/57 (46%), Gaps = 3/57 (5%)
Frame = -2
Query: 99 IVNRQHFTSLENNHPLLLFNP---HELVPQFEVNLYGDKDGPLPQIDVSNMTEPRLF 152
++ R T LE L +P + + F+++L D+DGPLP D P L+
Sbjct: 473 LLERSRITGLEVEEKLKGLSPVMFEDFIKPFQIDL--DEDGPLPHNDRVRYQTPNLY 309
>TC12913 homologue to UP|Q84L76 (Q84L76) Expansin precursor, partial (78%)
Length = 606
Score = 28.5 bits (62), Expect = 1.7
Identities = 18/50 (36%), Positives = 33/50 (66%), Gaps = 1/50 (2%)
Frame = -3
Query: 292 EGNQSGTISGDIEKEFYFRKGEIGDWANYLS-SSMVEKLSKVMEEKLNGS 340
+G+ SGT+ D+E+ ++ GEI A +++ +++V LSKV K++GS
Sbjct: 292 DGDDSGTVLSDLEEHWH---GEIEVRARWVAPATIVTGLSKVGWAKVSGS 152
>TC9508 weakly similar to UP|Q7XQG9 (Q7XQG9) OJ000114_01.8 protein, partial
(6%)
Length = 556
Score = 26.9 bits (58), Expect = 5.1
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 1/62 (1%)
Frame = -3
Query: 56 WCPSTLIQSVN-SFQNNFHAKDSDIVVASIPKSGTTWLKGLAYAIVNRQHFTSLENNHPL 114
W P + Q + FQ+ H + S I G+ + + A +NRQH T + HP
Sbjct: 305 WQPQSKYQQLPLPFQSEHHLQLSPEPFLLISSHGSHFPQAQLLA*LNRQH*TPSQTQHPQ 126
Query: 115 LL 116
L+
Sbjct: 125 LV 120
>BP040968
Length = 483
Score = 26.9 bits (58), Expect = 5.1
Identities = 16/35 (45%), Positives = 19/35 (53%)
Frame = -1
Query: 268 QEEENNGVIENIIKLCSFESMKEIEGNQSGTISGD 302
+EEE NG II+ S E + EG GTI GD
Sbjct: 408 EEEEVNGEGRLIIQKESGEESSQAEGASFGTIVGD 304
>BP044824
Length = 502
Score = 26.6 bits (57), Expect = 6.6
Identities = 14/46 (30%), Positives = 27/46 (58%), Gaps = 3/46 (6%)
Frame = +1
Query: 282 LCSFESMK---EIEGNQSGTISGDIEKEFYFRKGEIGDWANYLSSS 324
LC+ + +K E+EG +G +SGD++ F R+ + N +++S
Sbjct: 76 LCASKHVKIQCELEGKINGALSGDLDPRFIDRQKALEAAMNDINNS 213
>TC15913
Length = 471
Score = 26.2 bits (56), Expect = 8.7
Identities = 14/44 (31%), Positives = 20/44 (44%)
Frame = -3
Query: 29 HDYNNLILSLPRENGCETQYMYFFHGFWCPSTLIQSVNSFQNNF 72
H Y+NLI++ + +F+H F P L S F NF
Sbjct: 388 HHYSNLIIATKYSSQINI*NSHFYHSFSFPLFLFPSPPKFFLNF 257
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,622,085
Number of Sequences: 28460
Number of extensions: 102128
Number of successful extensions: 511
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 508
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 509
length of query: 348
length of database: 4,897,600
effective HSP length: 91
effective length of query: 257
effective length of database: 2,307,740
effective search space: 593089180
effective search space used: 593089180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148349.13