BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148347.5 - phase: 0
(118 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8536 weakly similar to UP|AAR92224 (AAR92224) Cystatin, partia... 86 2e-18
TC15594 similar to UP|CYTI_VIGUN (Q06445) Cysteine proteinase in... 39 3e-04
TC8168 similar to UP|O04720 (O04720) Cysteine proteinase inhibit... 38 5e-04
TC12363 similar to UP|O61067 (O61067) Circumsporozoite protein (... 25 4.4
TC14686 similar to UP|PORA_CUCSA (Q41249) Protochlorophyllide re... 25 4.4
BP066038 24 5.7
TC14635 similar to GB|AAO44074.1|28466931|BT004808 At5g11090 {Ar... 23 9.8
TC14093 similar to UP|Q940F8 (Q940F8) Sedoheptulose-bisphosphata... 23 9.8
AV771181 23 9.8
AV411094 23 9.8
TC10281 23 9.8
>TC8536 weakly similar to UP|AAR92224 (AAR92224) Cystatin, partial (69%)
Length = 543
Score = 85.5 bits (210), Expect = 2e-18
Identities = 51/123 (41%), Positives = 82/123 (66%), Gaps = 9/123 (7%)
Frame = +1
Query: 1 MRLESMVLVLVVLLA----FTAT--KQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRR 54
MR + +++V +++L+ F+A ++ +G +PI ++NDP V ++ANFAV EY+
Sbjct: 52 MRFDRVLIVSLLILSAVSGFSAALRQKGAFVGGYTPIKDLNDPHVKEIANFAVSEYD--- 222
Query: 55 RKPEEKLRLWKVIKGESQIVADGVNYRLTLSA---TKVYTSNTYEAIVLEWSLQHLRNLT 111
++ KLRL KV+ G+SQ+VA G NYRL ++A ++ ++ YEA+V E S +H RNLT
Sbjct: 223 KQSGAKLRLVKVVSGDSQVVA-GTNYRLVIAAKGGSRSAAASNYEALVYERSWEHFRNLT 399
Query: 112 SFK 114
SFK
Sbjct: 400 SFK 408
>TC15594 similar to UP|CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor
(Cystatin), complete
Length = 703
Score = 38.5 bits (88), Expect = 3e-04
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 1/93 (1%)
Frame = +2
Query: 25 IGNLSPI-NNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLT 83
+G S + N N ++ ++A FAV+E+N +K L KV+ Q+VA G Y++
Sbjct: 173 VGGASEVPGNQNSLEIENLARFAVEEHN---KKQNTLLEFGKVVSATQQVVA-GTLYKIK 340
Query: 84 LSATKVYTSNTYEAIVLEWSLQHLRNLTSFKLI 116
L YEA + E + + L F L+
Sbjct: 341 LEVNDGGKKQVYEAKIWEKPWLNFKELQEFNLV 439
>TC8168 similar to UP|O04720 (O04720) Cysteine proteinase inhibitor,
partial (55%)
Length = 808
Score = 37.7 bits (86), Expect = 5e-04
Identities = 28/80 (35%), Positives = 39/80 (48%)
Frame = +1
Query: 35 NDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATKVYTSNT 94
N ++ +A FAV E+NN K L +V+K + Q+VA G + LTL A
Sbjct: 349 NSLEIDALARFAVDEHNN---KQNALLEFGRVVKAQEQVVA-GSMHHLTLEAIDGGEKKI 516
Query: 95 YEAIVLEWSLQHLRNLTSFK 114
YEA V S + + L FK
Sbjct: 517 YEAKVWVKSWLNFKELQEFK 576
>TC12363 similar to UP|O61067 (O61067) Circumsporozoite protein (Fragment),
partial (4%)
Length = 719
Score = 24.6 bits (52), Expect = 4.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +2
Query: 53 RRRKPEEKLRLWKVIKGESQIVADG 77
RRR P K + W++I G + DG
Sbjct: 539 RRRTPGRKRQAWRIIPGLRRHALDG 613
>TC14686 similar to UP|PORA_CUCSA (Q41249) Protochlorophyllide reductase,
chloroplast precursor (PCR) (NADPH-protochlorophyllide
oxidoreductase) (POR) , partial (51%)
Length = 706
Score = 24.6 bits (52), Expect = 4.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -3
Query: 46 AVKEYNNRRRKPEEKLRLWK 65
A KE +++PEE L LWK
Sbjct: 143 AWKEKQEPKKQPEEPLELWK 84
>BP066038
Length = 445
Score = 24.3 bits (51), Expect = 5.7
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -1
Query: 36 DPKVIDVANFAVKEYNNRRRKPEEKLRLWK 65
D ++ID A KEY + K EEK R W+
Sbjct: 127 DSEIIDEILVAKKEYGKLKAKGEEKER-WR 41
>TC14635 similar to GB|AAO44074.1|28466931|BT004808 At5g11090 {Arabidopsis
thaliana;}, partial (71%)
Length = 1048
Score = 23.5 bits (49), Expect = 9.8
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = -1
Query: 53 RRRKPEEKLRLWKVIKG 69
R RK E++L W V+KG
Sbjct: 58 RERKREKRLGFWLVLKG 8
>TC14093 similar to UP|Q940F8 (Q940F8) Sedoheptulose-bisphosphatase, partial
(46%)
Length = 678
Score = 23.5 bits (49), Expect = 9.8
Identities = 8/23 (34%), Positives = 16/23 (68%)
Frame = +1
Query: 55 RKPEEKLRLWKVIKGESQIVADG 77
++PE KL +W++ KG ++ +G
Sbjct: 220 QEPEIKLFVWRITKGVIKVNTEG 288
>AV771181
Length = 435
Score = 23.5 bits (49), Expect = 9.8
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = +2
Query: 96 EAIVLEWSLQHLRNLTSFKLI 116
+A++ EW QHL+ T+ L+
Sbjct: 182 KALIREWHHQHLQTFTNHSLL 244
>AV411094
Length = 144
Score = 23.5 bits (49), Expect = 9.8
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = -2
Query: 22 AIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQ 72
++PI N +P P +A FAV ++ RRRK +++L+ + Q
Sbjct: 140 SLPIPNPTPT-----PPPTPMAAFAVPKWRTRRRK--ARIQLFSIFSSRYQ 9
>TC10281
Length = 510
Score = 23.5 bits (49), Expect = 9.8
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +2
Query: 7 VLVLVVLLAFTATKQAIPIGNLSPINNIN 35
VLV VVL+ TA +IP+ P NN++
Sbjct: 194 VLVSVVLMIVTALNTSIPL----PANNLD 268
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,683,061
Number of Sequences: 28460
Number of extensions: 16958
Number of successful extensions: 103
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of query: 118
length of database: 4,897,600
effective HSP length: 79
effective length of query: 39
effective length of database: 2,649,260
effective search space: 103321140
effective search space used: 103321140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)
Medicago: description of AC148347.5