BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148343.5 - phase: 0
(443 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18785 similar to UP|Q84PA3 (Q84PA3) ADP ribosylation GTPase-li... 248 1e-66
TC13107 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13... 241 2e-64
AV428070 145 1e-35
AV408991 87 4e-18
TC9890 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M... 80 7e-16
TC16744 similar to GB|AAP37738.1|30725432|BT008379 At4g13350 {Ar... 48 3e-06
CN825243 48 4e-06
BP043685 41 3e-04
TC16606 30 0.61
BP042272 30 0.80
BI420041 30 1.0
TC13209 similar to UP|Q9LMW1 (Q9LMW1) F5M15.14, partial (58%) 30 1.0
TC15883 similar to GB|AAP42727.1|30984528|BT008714 At4g14930 {Ar... 30 1.0
TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, pa... 29 1.4
TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-l... 29 1.4
TC7999 similar to UP|BAD01556 (BAD01556) ERF-like protein, parti... 29 1.4
AV419448 29 1.4
TC9022 similar to PIR|T46225|T46225 alpha NAC-like protein - Ara... 29 1.8
TC15581 similar to UP|AAR24654 (AAR24654) At5g56120, partial (74%) 28 3.0
TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin at... 28 3.0
>TC18785 similar to UP|Q84PA3 (Q84PA3) ADP ribosylation GTPase-like protein
(Fragment), partial (37%)
Length = 453
Score = 248 bits (634), Expect = 1e-66
Identities = 123/150 (82%), Positives = 136/150 (90%), Gaps = 1/150 (0%)
Frame = +3
Query: 169 GGDIRRNQSTGDVIGFVGNGVTPSSRSKSTEDIYTRTQLEASAANKEGFFAKKMAENESR 228
GGD+RRNQSTGDV+GF G G SRSKSTEDI+TRTQLEASAANKE FFA+KMAENESR
Sbjct: 3 GGDMRRNQSTGDVLGFGGGGGVVPSRSKSTEDIHTRTQLEASAANKESFFARKMAENESR 182
Query: 229 PEGLPPSQGGKYVGFGSSPGPAQRIS-PQNDYLSVVSEGIGKLSMVAQSATKEITAKVKD 287
P+GLPPSQGGKYVGFGSSP PAQR S PQNDY VVS+GIGKLS+V Q+ TKE+T+KVK+
Sbjct: 183 PDGLPPSQGGKYVGFGSSPAPAQRSSNPQNDYFDVVSQGIGKLSLVVQAGTKELTSKVKE 362
Query: 288 GGYDHKVNETVNIVTQKTSEIGQRTWGIMK 317
GGYD+KVNETVNIV+QKTSEIGQRTWGIMK
Sbjct: 363 GGYDYKVNETVNIVSQKTSEIGQRTWGIMK 452
>TC13107 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M22.5
{Arabidopsis thaliana;}, partial (29%)
Length = 465
Score = 241 bits (615), Expect = 2e-64
Identities = 114/142 (80%), Positives = 129/142 (90%)
Frame = +2
Query: 1 MAASRRLRELQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
MAASRRLRELQS P NKICVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 41 MAASRRLRELQSMPGNKICVDCTQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 220
Query: 61 MDSWSDLQIKKMEAGGNRNLNTFLSQYGISKETDIITKYNSNAASIYRDRIQAIAEGRSW 120
MD+WS++QIKKMEAGGN +LN FL++Y I KETDI+TKYN+NAA++YRDRIQAIA+GR W
Sbjct: 221 MDAWSEIQIKKMEAGGNDSLNAFLARYSIPKETDIVTKYNTNAATLYRDRIQAIADGRPW 400
Query: 121 RDPPVVKENASTRAGKGKPPLA 142
RDPPVVKEN+S KPPL+
Sbjct: 401 RDPPVVKENSS-----AKPPLS 451
>AV428070
Length = 356
Score = 145 bits (366), Expect = 1e-35
Identities = 68/70 (97%), Positives = 70/70 (99%)
Frame = +1
Query: 1 MAASRRLRELQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
MAASRRLR+LQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 145 MAASRRLRDLQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 324
Query: 61 MDSWSDLQIK 70
MDSWS+LQIK
Sbjct: 325 MDSWSELQIK 354
>AV408991
Length = 435
Score = 87.4 bits (215), Expect = 4e-18
Identities = 37/77 (48%), Positives = 52/77 (67%)
Frame = +1
Query: 7 LRELQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSD 66
L L P N+ C DC K P+WASV+ G+F+C++CSG HR LGVHIS VRS T+D+W
Sbjct: 151 LEGLLKLPENRECADCKAKGPRWASVNLGIFICMQCSGIHRSLGVHISKVRSATLDTWLP 330
Query: 67 LQIKKMEAGGNRNLNTF 83
Q+ +++ GN N++
Sbjct: 331 EQVAFIQSMGNEKANSY 381
>TC9890 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M22.5
{Arabidopsis thaliana;}, partial (6%)
Length = 547
Score = 80.1 bits (196), Expect = 7e-16
Identities = 34/43 (79%), Positives = 38/43 (88%)
Frame = +2
Query: 400 GSAPHNNDDWAGWDDAKDDDDEFDDKSFGNNGTSGSGWTGGGF 442
GS+PHNND WAGWDDAKDDD FD+K+ +NGTSGSGWTGGGF
Sbjct: 2 GSSPHNNDSWAGWDDAKDDD--FDNKASDHNGTSGSGWTGGGF 124
>TC16744 similar to GB|AAP37738.1|30725432|BT008379 At4g13350 {Arabidopsis
thaliana;}, partial (18%)
Length = 687
Score = 48.1 bits (113), Expect = 3e-06
Identities = 22/74 (29%), Positives = 41/74 (54%)
Frame = +2
Query: 5 RRLRELQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSW 64
R +R L SN+ C++C+ PQ+ ++ F+C CSG HR V+S++M +
Sbjct: 383 RVIRGLLKLESNRRCINCNSLGPQYVCTNFWTFVCTNCSGIHREF---THRVKSISMAKF 553
Query: 65 SDLQIKKMEAGGNR 78
+ ++ ++ GGN+
Sbjct: 554 TSQEVIALQEGGNQ 595
>CN825243
Length = 315
Score = 47.8 bits (112), Expect = 4e-06
Identities = 27/78 (34%), Positives = 39/78 (49%), Gaps = 5/78 (6%)
Frame = +1
Query: 58 SVTMDSWSDLQIKKMEAGGNRNLNTFLSQYGISKETDIITKYNSNAASIYRDRI-----Q 112
+ +DSWS Q+K M GGN F Q+G + I KY S AA +YR + +
Sbjct: 1 ATNLDSWSPEQLKTMSFGGNGRAQVFFKQHGWTDGGKIEAKYTSRAAELYRQILSKEVAK 180
Query: 113 AIAEGRSWRDPPVVKENA 130
++AE S PV ++A
Sbjct: 181 SMAEEASLLSSPVASQSA 234
>BP043685
Length = 433
Score = 41.2 bits (95), Expect = 3e-04
Identities = 15/41 (36%), Positives = 23/41 (55%)
Frame = +1
Query: 7 LRELQSEPSNKICVDCSQKNPQWASVSYGVFMCLECSGKHR 47
+R L P N+ C++C PQ+ ++ F+C CSG HR
Sbjct: 298 IRGLMKLPPNRRCINCDNLGPQYVCTNFWTFICTTCSGIHR 420
>TC16606
Length = 532
Score = 30.4 bits (67), Expect = 0.61
Identities = 21/65 (32%), Positives = 28/65 (42%), Gaps = 3/65 (4%)
Frame = +1
Query: 139 PPLAAASN---GGGWDDNWDNDDGDSYGYGSRGGGDIRRNQSTGDVIGFVGNGVTPSSRS 195
P A+A+N GWD WD+D D+ +S G V G NG+T S +
Sbjct: 40 PVSASATNVESAEGWDQGWDDDWDDNVAV-----------KSPGAVRGISANGLTSRSAN 186
Query: 196 KSTED 200
K D
Sbjct: 187 KDGWD 201
Score = 30.0 bits (66), Expect = 0.80
Identities = 12/36 (33%), Positives = 17/36 (46%)
Frame = +1
Query: 120 WRDPPVVKENASTRAGKGKPPLAAASNGGGWDDNWD 155
W D VK + R + ++N GWD+NWD
Sbjct: 106 WDDNVAVKSPGAVRGISANGLTSRSANKDGWDNNWD 213
>BP042272
Length = 471
Score = 30.0 bits (66), Expect = 0.80
Identities = 19/60 (31%), Positives = 27/60 (44%)
Frame = +1
Query: 145 SNGGGWDDNWDNDDGDSYGYGSRGGGDIRRNQSTGDVIGFVGNGVTPSSRSKSTEDIYTR 204
S+ GGW D+DDG Y GGGD R G G + S S++ + ++R
Sbjct: 295 SSRGGWGGGRDSDDGFGDQYRRGGGGDQYRR----------GGGSSSSRNSRTGGNSWSR 444
>BI420041
Length = 452
Score = 29.6 bits (65), Expect = 1.0
Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = +2
Query: 403 PHNNDDWAG---WDDAKDDDDEFDDKSFGNNGTSGSGWTGGG 441
P N++D G +DD + +++EF+D+ + +G G +GGG
Sbjct: 284 PLNDEDEDGDEYYDDDEQEEEEFEDEDEDSVPYAGDGGSGGG 409
>TC13209 similar to UP|Q9LMW1 (Q9LMW1) F5M15.14, partial (58%)
Length = 750
Score = 29.6 bits (65), Expect = 1.0
Identities = 27/108 (25%), Positives = 38/108 (35%), Gaps = 6/108 (5%)
Frame = +3
Query: 290 YDHKVNETVNIVTQKTSEIGQRTWGIMKGVMALASQKVEEFTKDYPDGNSDN----WPRN 345
+D ++ TV G W I + L S ++E T D + WP
Sbjct: 297 FDQRIFSTVY---SNGESYGAAIWQIPELYGELNSPQLERITSLDTDASKIKCILWWPSG 467
Query: 346 ENDRH-DFNQENKG-WNSSTSTREGQPSSGGQTNTYHSNSWDDWDNQD 391
+D+ N+EN WN S + Q S H S WD D
Sbjct: 468 RHDKLISINEENLCLWNLDVSKKTAQVQSKDSAGVLHKLSGGAWDPHD 611
>TC15883 similar to GB|AAP42727.1|30984528|BT008714 At4g14930 {Arabidopsis
thaliana;}, partial (35%)
Length = 1011
Score = 29.6 bits (65), Expect = 1.0
Identities = 27/88 (30%), Positives = 37/88 (41%), Gaps = 8/88 (9%)
Frame = +2
Query: 347 NDRHDFNQENK-----GWNSSTSTREGQPSSGGQTNTYHSNSWDDWDNQDTRKEVPAKGS 401
ND + ++ K GW TS EG+ + TNT D D DT K V A
Sbjct: 377 NDGYKLTKQGKSIVRMGWKQVTSETEGRKMTSDMTNT-------DTDT-DTPKNVGASSV 532
Query: 402 APHNND---DWAGWDDAKDDDDEFDDKS 426
+P + + G+ DDDD+ D S
Sbjct: 533 SPEHLSFVREVRGYQ--LDDDDDTDHSS 610
>TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, partial (3%)
Length = 504
Score = 29.3 bits (64), Expect = 1.4
Identities = 28/121 (23%), Positives = 45/121 (37%), Gaps = 15/121 (12%)
Frame = +3
Query: 323 ASQKVEEFTKDYP-DGNSDNWPRNENDRHDFNQENKGWNSSTSTREGQPSSGGQTNTYHS 381
AS +V E + P D +SD D D + ++ G + G + H
Sbjct: 15 ASARVYEIGRCRPTDDDSDPDDAESEDEEDDDDDDDGDDEDPLLGPGLGRESDSDDMSHD 194
Query: 382 N--SWDDWDNQDT------------RKEVPAKGSAPHNNDDWAGWDDAKDDDDEFDDKSF 427
+ S D D++D + G ++DD + A DD++FDD F
Sbjct: 195 DYDSASDIDDEDADFILDDLDFDGGTGLLEIVGDRDEDDDDSQELESASSDDEDFDDNDF 374
Query: 428 G 428
G
Sbjct: 375 G 377
>TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-like
protein, partial (57%)
Length = 1141
Score = 29.3 bits (64), Expect = 1.4
Identities = 19/51 (37%), Positives = 21/51 (40%), Gaps = 7/51 (13%)
Frame = +3
Query: 145 SNGGGWDDNW-------DNDDGDSYGYGSRGGGDIRRNQSTGDVIGFVGNG 188
S GGG+D + D G YG G GGG STG G G G
Sbjct: 303 SGGGGYDSGYNKTAYSTDEPSGGGYGTGGAGGG-----YSTGGGYGTGGGG 440
>TC7999 similar to UP|BAD01556 (BAD01556) ERF-like protein, partial (11%)
Length = 690
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/68 (32%), Positives = 31/68 (45%), Gaps = 4/68 (5%)
Frame = +2
Query: 151 DDNWDNDDGDSYGYGSRGGG----DIRRNQSTGDVIGFVGNGVTPSSRSKSTEDIYTRTQ 206
D+N + G G GS GGG + ++ G+ G+ G G+ + K TE T
Sbjct: 50 DENSGSGSGLGSGTGSEGGGYSPMEFMGSEQIGN--GYFGGGLKEKEKEKETEQ-KTEVV 220
Query: 207 LEASAANK 214
EA AA K
Sbjct: 221 EEAEAAAK 244
>AV419448
Length = 382
Score = 29.3 bits (64), Expect = 1.4
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = -2
Query: 127 KENASTRAGKGKPPLAAASNGGGWDDNWDNDDGDSYGYGSRGGGDIR 173
K+NA+ R G G +A GGG + D G G GG+ R
Sbjct: 213 KDNAAERGGVGADGVAVGEEGGGAEAEVHEDAVGDGGVGGWSGGEGR 73
>TC9022 similar to PIR|T46225|T46225 alpha NAC-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (75%)
Length = 1083
Score = 28.9 bits (63), Expect = 1.8
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +3
Query: 390 QDTRKEVPAKGSAPHNNDDWAGWDDAKDDDDEFDDKSFGNNGTS 433
Q + P ++ D A D+ +DDDDE DDK G G S
Sbjct: 192 QPQEDDAPIVEDVKDDDKDEADDDEDEDDDDEDDDKEDGALGGS 323
>TC15581 similar to UP|AAR24654 (AAR24654) At5g56120, partial (74%)
Length = 1462
Score = 28.1 bits (61), Expect = 3.0
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +1
Query: 313 WGIMKGVMALASQKVEEFTKDYPDGNSDNWPRNENDRHDF 352
+GI + L S+ EF +++ G S W R D H F
Sbjct: 904 FGISTSIRDLLSKLALEFAENFKFGKS*GWSRCHKDHHHF 1023
>TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (3%)
Length = 1235
Score = 28.1 bits (61), Expect = 3.0
Identities = 30/113 (26%), Positives = 42/113 (36%), Gaps = 13/113 (11%)
Frame = +1
Query: 130 ASTRAGKGKPPLAAASNGGGWDDNWDNDDGDSYGYGSRGGGDIRRNQ------------- 176
+S+ GKG +A G G D+D + + GGD +Q
Sbjct: 490 SSSSGGKGGSKFGSAKKGFGGKAGKDHDGEVKEMWDTIAGGDSEDDQEGVRNLDDDNFID 669
Query: 177 STGDVIGFVGNGVTPSSRSKSTEDIYTRTQLEASAANKEGFFAKKMAENESRP 229
TG GF GN PSS ++ + E + K G KK +NE P
Sbjct: 670 DTGVEPGFYGNYNEPSSPGEAPQAEEGEEDDEINDLFKMG---KKKKKNERSP 819
Score = 27.3 bits (59), Expect = 5.2
Identities = 24/104 (23%), Positives = 37/104 (35%)
Frame = +1
Query: 326 KVEEFTKDYPDGNSDNWPRNENDRHDFNQENKGWNSSTSTREGQPSSGGQTNTYHSNSWD 385
+VEE +D+P + + R D K TR G SSGG+ + ++
Sbjct: 367 EVEE--EDFPVRSDEGEGRKRKKEKDGGSGKKEKRLKGDTRFGSSSSGGKGGSKFGSA-- 534
Query: 386 DWDNQDTRKEVPAKGSAPHNNDDWAGWDDAKDDDDEFDDKSFGN 429
+K K H+ + WD D E D + N
Sbjct: 535 -------KKGFGGKAGKDHDGEVKEMWDTIAGGDSEDDQEGVRN 645
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.129 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,485,207
Number of Sequences: 28460
Number of extensions: 104939
Number of successful extensions: 656
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of query: 443
length of database: 4,897,600
effective HSP length: 93
effective length of query: 350
effective length of database: 2,250,820
effective search space: 787787000
effective search space used: 787787000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148343.5