BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148291.4 + phase: 0 /pseudo
(659 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089582 107 8e-41
AV427422 140 6e-34
BP055130 110 7e-26
BF177840 90 1e-18
BP054462 67 7e-12
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 56 2e-08
BP046863 44 8e-05
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 42 2e-04
BP048192 31 0.55
TC13084 30 1.2
AV418386 28 6.1
TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehyd... 27 8.0
>AU089582
Length = 383
Score = 107 bits (268), Expect(2) = 8e-41
Identities = 49/83 (59%), Positives = 64/83 (77%)
Frame = +2
Query: 447 AQLAEIYIHSVVKLHGVPASIVSDRDPRFTSRFWKRLQDALGSKLRLSSAYHPQTDGQSE 506
+Q A+IY+ +V LHGVP SI+SDR +FTS FW+ Q ALG++L++S+A+HPQTDGQSE
Sbjct: 14 SQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSE 193
Query: 507 RTIQSLEDLLRVCVLEQGGAWDS 529
RTIQ LED+LR CV + G S
Sbjct: 194 RTIQILEDMLRACVXDLRGXLGS 262
Score = 77.0 bits (188), Expect(2) = 8e-41
Identities = 33/45 (73%), Positives = 38/45 (84%)
Frame = +3
Query: 525 GAWDSHLPLIEFTYNNSYHSSIGMAPFEALYGRRCRTPLCWFESG 569
G+WD +L L+EF YNNSY SSI MAPFEALYGRRCR+P+ WFE G
Sbjct: 249 GSWDQYLSLMEFAYNNSYRSSI*MAPFEALYGRRCRSPIGWFEVG 383
>AV427422
Length = 417
Score = 140 bits (353), Expect = 6e-34
Identities = 67/139 (48%), Positives = 94/139 (67%)
Frame = +1
Query: 412 TSLPNTPRGHDAIWVVVDRLTKSAHFIPININYPVAQLAEIYIHSVVKLHGVPASIVSDR 471
T LP + +G++A+ VVVDRL+K +HF+P+ Y +A+I++ VV+LHGVP SIVSDR
Sbjct: 4 TGLPKS-KGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDR 180
Query: 472 DPRFTSRFWKRLQDALGSKLRLSSAYHPQTDGQSERTIQSLEDLLRVCVLEQGGAWDSHL 531
DP F S FWK L G+KL++S+AYHP++DGQ+E + LE LR + +Q +W +
Sbjct: 181 DPLFMSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWV 360
Query: 532 PLIEFTYNNSYHSSIGMAP 550
P E+ YN SYH S G P
Sbjct: 361 PWAEYWYNTSYHVSTGQTP 417
>BP055130
Length = 567
Score = 110 bits (275), Expect(2) = 7e-26
Identities = 57/112 (50%), Positives = 72/112 (63%)
Frame = +2
Query: 420 GHDAIWVVVDRLTKSAHFIPININYPVAQLAEIYIHSVVKLHGVPASIVSDRDPRFTSRF 479
G I VVVDRL+K HF P NY +Q+AE ++ ++VKLHG+P +IVSDRD FTS F
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 480 WKRLQDALGSKLRLSSAYHPQTDGQSERTIQSLEDLLRVCVLEQGGAWDSHL 531
WK G+ L +SS+YHPQTDGQ+E + LE LR V E W S+L
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
Score = 24.3 bits (51), Expect(2) = 7e-26
Identities = 9/17 (52%), Positives = 13/17 (75%)
Frame = +3
Query: 531 LPLIEFTYNNSYHSSIG 547
LP E+ YN+S+ +SIG
Sbjct: 516 LPWAEYCYNSSFQTSIG 566
>BF177840
Length = 410
Score = 90.1 bits (222), Expect = 1e-18
Identities = 51/129 (39%), Positives = 71/129 (54%)
Frame = +2
Query: 466 SIVSDRDPRFTSRFWKRLQDALGSKLRLSSAYHPQTDGQSERTIQSLEDLLRVCVLEQGG 525
SIVSDRD +F S FW+ L +G+KL S+ HPQTDGQ+E ++L LLR +
Sbjct: 14 SIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNLK 193
Query: 526 AWDSHLPLIEFTYNNSYHSSIGMAPFEALYGRRCRTPLCWFESGESVVLGPDLVHETTEK 585
W++ LP IEF YN HS+ +PFE +YG TPL + +L E
Sbjct: 194 MWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPNTYLLKHKDGKAKAEF 373
Query: 586 VRLIRERMK 594
V+ + E++K
Sbjct: 374 VKRLHEKIK 400
>BP054462
Length = 422
Score = 67.4 bits (163), Expect = 7e-12
Identities = 38/116 (32%), Positives = 64/116 (54%), Gaps = 1/116 (0%)
Frame = +1
Query: 539 NNSYHSSIGMAPFEALYGRRCRTPLCWFESGESVVLGPDLVHETTEKVRLIRERMKASQS 598
N++Y+ S M+PF+ALYGR L + DL E + +R + SQ
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 599 RQKSYHDKRRKELEFQEGDHVFLRVTPLTGVGRALK-SRKLTPKFIGPYQISDRVG 653
++Y +K+R+++++Q GD VFL++ P A K + KL+P++ GPY I ++G
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIG 348
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 56.2 bits (134), Expect = 2e-08
Identities = 30/90 (33%), Positives = 48/90 (53%)
Frame = +2
Query: 474 RFTSRFWKRLQDALGSKLRLSSAYHPQTDGQSERTIQSLEDLLRVCVLEQGGAWDSHLPL 533
+FTS+ + D +G ++R SS HPQT+GQ+E + + ++ + E G W LP+
Sbjct: 20 QFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPI 199
Query: 534 IEFTYNNSYHSSIGMAPFEALYGRRCRTPL 563
+ ++YN SSI PF YG P+
Sbjct: 200 VLWSYNTMPQSSIKETPF*LTYGADTMLPV 289
>BP046863
Length = 580
Score = 43.9 bits (102), Expect = 8e-05
Identities = 22/76 (28%), Positives = 46/76 (59%), Gaps = 1/76 (1%)
Frame = +1
Query: 578 LVHETTEKVRLIRERMKASQSRQKSYHDKRRKELEFQEGDHVFLRVTPLTGVGRAL-KSR 636
L+ E + +R + +Q + ++ +K R+ +++Q G+ VFL++ P A K++
Sbjct: 274 LIEERDALLLELRGNLLKAQDQMRAQANKHRRYVDYQVGNWVFLKLQPYKLQNLAQRKNQ 453
Query: 637 KLTPKFIGPYQISDRV 652
KL+P+F GP+++ +RV
Sbjct: 454 KLSPRFYGPFKVLERV 501
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 42.4 bits (98), Expect = 2e-04
Identities = 22/45 (48%), Positives = 30/45 (65%), Gaps = 7/45 (15%)
Frame = +3
Query: 622 RVTPLTGVGRALKSRKLTPKFIGPYQISDRVG-------LPPHLS 659
RV+P+ GV R K KL+P+FIGP+++ +RVG LPP LS
Sbjct: 3 RVSPMKGVLRFGKKGKLSPRFIGPFEVLERVGSVSYRLALPPDLS 137
>BP048192
Length = 551
Score = 31.2 bits (69), Expect = 0.55
Identities = 13/40 (32%), Positives = 24/40 (59%), Gaps = 3/40 (7%)
Frame = +3
Query: 183 FEVFSDHKSLKYLFDQ---KELNMRQRRWLELLKDYDFGL 219
F ++D+ ++ Y Q KE+++ + RW +KDYD G+
Sbjct: 198 FLTYADNNAVGYFIKQGFTKEIHLEKERWQGYIKDYDGGI 317
>TC13084
Length = 508
Score = 30.0 bits (66), Expect = 1.2
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +1
Query: 301 NDTEDSDFKVDDQGVLRFRGKVCI 324
N TEDSD+ +++ RFRG+ CI
Sbjct: 160 NMTEDSDYDEEEEDYPRFRGRYCI 231
>AV418386
Length = 352
Score = 27.7 bits (60), Expect = 6.1
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -1
Query: 125 LGLGGVLMQDGKVVAYASRQ 144
LGLGG L +G VVAY+ R+
Sbjct: 148 LGLGGFLSTEGNVVAYSHRR 89
>TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (62%)
Length = 809
Score = 27.3 bits (59), Expect = 8.0
Identities = 11/46 (23%), Positives = 23/46 (49%)
Frame = -2
Query: 560 RTPLCWFESGESVVLGPDLVHETTEKVRLIRERMKASQSRQKSYHD 605
+ LCWF ++++ PD + +RL A+ +++ S H+
Sbjct: 268 KATLCWFSIYINIIVIPDFLFAVLLNIRLACRAFFAASNKEASSHN 131
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,754,744
Number of Sequences: 28460
Number of extensions: 161056
Number of successful extensions: 717
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 716
length of query: 659
length of database: 4,897,600
effective HSP length: 96
effective length of query: 563
effective length of database: 2,165,440
effective search space: 1219142720
effective search space used: 1219142720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148291.4