BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.3 + phase: 0 /pseudo
(456 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor,... 449 e-127
TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02 ... 375 e-104
TC9432 weakly similar to UP|Q9M3X6 (Q9M3X6) Convicilin precursor... 76 1e-14
AU089496 43 3e-10
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 47 5e-06
TC10913 similar to UP|Q8VZH3 (Q8VZH3) At2g13540/T10F5.8, partial... 33 0.13
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 31 0.37
AV772572 30 0.63
TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like pr... 30 0.83
TC9585 similar to UP|O24078 (O24078) Protein phosphatase 2C, par... 30 1.1
TC13685 similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800),... 29 1.4
BP038960 29 1.8
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 29 1.8
AV411485 28 2.4
BP040798 28 2.4
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 28 2.4
AI967315 28 3.1
BP058107 28 4.1
CN825310 28 4.1
TC14892 similar to UP|Q9Z0W6 (Q9Z0W6) Pax transcription activati... 28 4.1
>TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor, partial
(74%)
Length = 1591
Score = 449 bits (1155), Expect = e-127
Identities = 224/378 (59%), Positives = 298/378 (78%)
Frame = +2
Query: 76 QENPFFFNANRFQTLFENENGHIRLLQRFDKRSKIFENLQNYRLLEYHSKPHTLFLPQHN 135
+ NPF F ++RFQT FENE+GH+R+LQRFD+RSK+FENLQNYR+LE+ +KP TL LP HN
Sbjct: 467 RRNPFHFRSSRFQTHFENEHGHVRVLQRFDERSKLFENLQNYRILEFKAKPQTLVLPHHN 646
Query: 136 DADFILAVLSGKAILTVLNPNDRNSFNLERGDTIKLPAGSIAYLANRADNEDLRVLDLAI 195
DAD I+ +LSG+AILT++NPNDR+S+NLE GD + +PAG+ AYLANR ++E+LRV+ L I
Sbjct: 647 DADSIIVILSGRAILTIVNPNDRDSYNLESGDALVIPAGATAYLANRDNDENLRVVKLLI 826
Query: 196 PVNRPGQFQSFSLSGNQNQQSFFSGFSKNILEAAFNSNYEEIERVLIEEQEQEPRHRRGL 255
P+NRPGQ+Q F S ++ Q+S+ +GFS+NILEA+FN+ Y+EIERVL++ +EQ
Sbjct: 827 PINRPGQYQPFFPSSSETQESYLNGFSRNILEASFNAGYDEIERVLLQREEQ-------- 982
Query: 256 RDRRHKQSQEANVIVKVSREQIEELSRHAKSSSRRSASSESASRRSASSESAPFNLRSRE 315
R +QSQE VIVK S++QI++LSRHAKSS SR+ +SS+S PFNLRS +
Sbjct: 983 ---RGEQSQEQGVIVKASQDQIQQLSRHAKSS----------SRKRSSSKSEPFNLRSSK 1123
Query: 316 PIYSNEFGNFFEITPEKNPQLQDLDILVNYAEIREGSLLLPHFNSRATVIVAVEEGKGEF 375
PI SN+FG FEITPEKN QL+DLDIL++ A+I+EGS+ LPH++S +T+I+ V EG+GE
Sbjct: 1124PISSNKFGKLFEITPEKNQQLRDLDILLSEAQIKEGSIFLPHYHSTSTLILVVNEGRGEL 1303
Query: 376 ELVGQRNENQQEQREYEEDEQEEERSQQVQRYRARLTPGDVYVIPAGYPNVVKASSDLSL 435
ELV QR +Q+QR EE+++EE+ + QR+RARL+PGDV VIPA +P V ASSDL+L
Sbjct: 1304ELVAQR---RQQQRGQEEEQEEEQPRIEAQRFRARLSPGDVIVIPASHPFAVTASSDLNL 1474
Query: 436 LGFGINAENNQRSFLAAK 453
L FGINAENNQR+FLA +
Sbjct: 1475LAFGINAENNQRNFLAGR 1528
Score = 33.5 bits (75), Expect = 0.075
Identities = 21/42 (50%), Positives = 29/42 (69%), Gaps = 3/42 (7%)
Frame = +2
Query: 41 IIQIIMA---IKAPFQLLMLLGIFFLASVCVSSRSDHDQENP 79
+IQ+IMA IKA LL+LLGI FL SV +++ S +E+P
Sbjct: 23 LIQLIMASAKIKARLPLLLLLGILFLVSVSLATAS-RQREDP 145
>TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02
(Fragment), partial (67%)
Length = 1328
Score = 375 bits (962), Expect = e-104
Identities = 190/332 (57%), Positives = 246/332 (73%), Gaps = 2/332 (0%)
Frame = +2
Query: 76 QENPFFF--NANRFQTLFENENGHIRLLQRFDKRSKIFENLQNYRLLEYHSKPHTLFLPQ 133
+ NPF+F +++RFQT F+NE G++R+LQRFD+RSK+FENLQNYR+ E+ +KPHT+ LP
Sbjct: 410 RRNPFYFRSSSSRFQTRFQNEYGYVRVLQRFDERSKLFENLQNYRIFEFKAKPHTVVLPH 589
Query: 134 HNDADFILAVLSGKAILTVLNPNDRNSFNLERGDTIKLPAGSIAYLANRADNEDLRVLDL 193
HNDAD I+ +LSGKAI+T++NPNDR SFNLERGD + PAG+IAY+AN DNE+LR+ +
Sbjct: 590 HNDADSIVVILSGKAIITLVNPNDRESFNLERGDVLVHPAGTIAYVANHDDNENLRIAKI 769
Query: 194 AIPVNRPGQFQSFSLSGNQNQQSFFSGFSKNILEAAFNSNYEEIERVLIEEQEQEPRHRR 253
IPVNRPG+FQ+F S + Q+S+ +GFS+NILEA+FN+ Y EIERVL+
Sbjct: 770 IIPVNRPGEFQAFYPSNTEPQESYLNGFSRNILEASFNAEYNEIERVLL----------- 916
Query: 254 GLRDRRHKQSQEANVIVKVSREQIEELSRHAKSSSRRSASSESASRRSASSESAPFNLRS 313
R +Q QE +IVKVSR+ I++LSRHAKSS SR+ SSE PFNLRS
Sbjct: 917 ----RGGEQRQEQGLIVKVSRDLIQQLSRHAKSS----------SRKRTSSEPEPFNLRS 1054
Query: 314 REPIYSNEFGNFFEITPEKNPQLQDLDILVNYAEIREGSLLLPHFNSRATVIVAVEEGKG 373
R+PIYSNEFG FEI P +N Q D DI ++ EIREGS+ LPH+NSR+TVI+ V EG+G
Sbjct: 1055RDPIYSNEFGKHFEINPNRNSQPTDFDIFLSSTEIREGSIFLPHYNSRSTVILVVNEGRG 1234
Query: 374 EFELVGQRNENQQEQREYEEDEQEEERSQQVQ 405
EFELV QR QQ+QR EEDE+EEE +++
Sbjct: 1235EFELVAQR--KQQQQRRNEEDEEEEEEQPRIE 1324
Score = 35.4 bits (80), Expect = 0.020
Identities = 54/225 (24%), Positives = 77/225 (34%), Gaps = 46/225 (20%)
Frame = +2
Query: 244 EQEQEPRHRRGLRDRRHKQSQE-------------------ANVIVKVSREQIEELSRHA 284
E+ QEP HR R K+ E + RE++ E + A
Sbjct: 110 ERHQEPGHRERARQEGEKEEDERQPWWQPGRGEEEGEWRGSRRLEDPDERERVRERTERA 289
Query: 285 KSSSR-------------RSASSESASRRSASSESA-------PFNLRSREPIYSNEFGN 324
K R R + E S S+SSES+ PF RS + F N
Sbjct: 290 KKWRRETEERDTPRRPHHRESEEEEGSSSSSSSESSRRSQRRNPFYFRSSSSRFQTRFQN 469
Query: 325 ---FFEITPEKNPQLQDLDILVNYA----EIREGSLLLPHFNSRATVIVAVEEGKGEFEL 377
+ + + + + + L NY + + +++LPH N A IV + GK L
Sbjct: 470 EYGYVRVLQRFDERSKLFENLQNYRIFEFKAKPHTVVLPHHND-ADSIVVILSGKAIITL 646
Query: 378 VGQRNENQQEQREYEEDEQEEERSQQVQRYRARLTPGDVYVIPAG 422
V N N +E E GDV V PAG
Sbjct: 647 V---NPNDRESFNLER--------------------GDVLVHPAG 712
>TC9432 weakly similar to UP|Q9M3X6 (Q9M3X6) Convicilin precursor, partial
(18%)
Length = 516
Score = 75.9 bits (185), Expect = 1e-14
Identities = 35/60 (58%), Positives = 47/60 (78%)
Frame = +1
Query: 392 EEDEQEEERSQQVQRYRARLTPGDVYVIPAGYPNVVKASSDLSLLGFGINAENNQRSFLA 451
+E+E+E + + QR+RARL+PGDV VIPAG+P + ASSDL+ + FGI+AENN R FLA
Sbjct: 1 DEEEEEVQPRIEAQRFRARLSPGDVVVIPAGHPVAINASSDLNFIAFGIDAENNPRHFLA 180
>AU089496
Length = 438
Score = 42.7 bits (99), Expect(2) = 3e-10
Identities = 19/61 (31%), Positives = 36/61 (58%)
Frame = +3
Query: 131 LPQHNDADFILAVLSGKAILTVLNPNDRNSFNLERGDTIKLPAGSIAYLANRADNEDLRV 190
+PQ+ D+ ++ V SG+A + + + L+RGD ++PAGS YL N +++ L +
Sbjct: 9 VPQYLDSSLVMFVRSGEAKVAFIYKDKLAERKLKRGDVYEIPAGSAFYLVNTGESQKLHI 188
Query: 191 L 191
+
Sbjct: 189 I 191
Score = 38.5 bits (88), Expect(2) = 3e-10
Identities = 17/46 (36%), Positives = 24/46 (51%)
Frame = +1
Query: 201 GQFQSFSLSGNQNQQSFFSGFSKNILEAAFNSNYEEIERVLIEEQE 246
G F+SF + G N S SGF ILE FN+ E+ ++ + E
Sbjct: 226 GIFESFYIGGGANPASVLSGFEPEILETVFNATGPELRKIFTRQHE 363
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 47.4 bits (111), Expect = 5e-06
Identities = 39/144 (27%), Positives = 58/144 (40%), Gaps = 8/144 (5%)
Frame = +1
Query: 243 EEQEQEPRHRRGLRDR-RHKQSQEANVIVKVSREQIEELSRHAKSSSRRSASSESASRRS 301
E +E+E R RG R R +H Q +E E+ EE SR S R S +
Sbjct: 682 EREEEEEREERGPRGRGKHWQREE---------EEEEEESRRTIPSRRESRGRGGCRTSN 834
Query: 302 ASSES-------APFNLRSREPIYSNEFGNFFEITPEKNPQLQDLDILVNYAEIREGSLL 354
E+ N SR +Y+ G +I P L+ L + Y + + +
Sbjct: 835 GLEENFCTLKIHENINRPSRADLYNPRAGRISDINSLTLPILRFLGLSAEYVNLYQNGIY 1014
Query: 355 LPHFNSRATVIVAVEEGKGEFELV 378
PH+N A I+ V G+G +V
Sbjct: 1015GPHWNINANSIIYVVRGRGRVRIV 1086
>TC10913 similar to UP|Q8VZH3 (Q8VZH3) At2g13540/T10F5.8, partial (18%)
Length = 560
Score = 32.7 bits (73), Expect = 0.13
Identities = 16/39 (41%), Positives = 26/39 (66%)
Frame = -3
Query: 281 SRHAKSSSRRSASSESASRRSASSESAPFNLRSREPIYS 319
S HA+++S+ S+ S SRRSA + + ++L+S E YS
Sbjct: 261 SEHARTNSKISSWERSGSRRSAPKQVSMWSLKSEEAPYS 145
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 31.2 bits (69), Expect = 0.37
Identities = 15/65 (23%), Positives = 35/65 (53%)
Frame = +1
Query: 342 LVNYAEIREGSLLLPHFNSRATVIVAVEEGKGEFELVGQRNENQQEQREYEEDEQEEERS 401
++NYA G PH +S T++ A + + ++ Q+++ Q Q + +Q++++
Sbjct: 7 MMNYASFGGGQQFYPHNHSMHTLLAA--QQFQQLQIHPQKHQQHQHQFQQPIQQQQQQQQ 180
Query: 402 QQVQR 406
Q +Q+
Sbjct: 181 QPIQQ 195
>AV772572
Length = 491
Score = 30.4 bits (67), Expect = 0.63
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = -1
Query: 284 AKSSSRRSASSESASRRSASSESAPFNLRSRE 315
A S + RS S+ES RRS S SAP N +S +
Sbjct: 275 ATSPASRSTSAESTVRRSGSVRSAPRNTQSHQ 180
>TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like protein
{Arabidopsis thaliana;} , partial (56%)
Length = 434
Score = 30.0 bits (66), Expect = 0.83
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +3
Query: 331 EKNPQLQDLDILVNYAEIREGSLLLPHFNSRATVIVAVEEGK 372
EK P L L + ++ + G L PH + RAT +V V EG+
Sbjct: 285 EKIPGLNTLGVSISRIDYAPGGLNPPHTHPRATEVVFVLEGQ 410
>TC9585 similar to UP|O24078 (O24078) Protein phosphatase 2C, partial (31%)
Length = 502
Score = 29.6 bits (65), Expect = 1.1
Identities = 16/36 (44%), Positives = 22/36 (60%)
Frame = +3
Query: 281 SRHAKSSSRRSASSESASRRSASSESAPFNLRSREP 316
S A S + SAS+ S S ++SS S+PF LR +P
Sbjct: 204 SSTASSCASASASASSPSAAASSSPSSPFRLRLPKP 311
>TC13685 similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800), partial
(37%)
Length = 585
Score = 29.3 bits (64), Expect = 1.4
Identities = 15/36 (41%), Positives = 22/36 (60%)
Frame = +1
Query: 365 IVAVEEGKGEFELVGQRNENQQEQREYEEDEQEEER 400
+ A EEG+GE QR+ NQQ+ RE E+ ++R
Sbjct: 379 VSAAEEGEGEGGEQRQRH*NQQQSREEN*REENQQR 486
>BP038960
Length = 524
Score = 28.9 bits (63), Expect = 1.8
Identities = 16/58 (27%), Positives = 32/58 (54%), Gaps = 3/58 (5%)
Frame = +3
Query: 372 KGEFELVGQRNENQQEQREYEEDEQEEERSQQVQRYRARLT---PGDVYVIPAGYPNV 426
KGE E + ++++E +Y++DE+EEE ++ + R+ PG ++ G+ V
Sbjct: 276 KGEAEQP-RVEDSEEEAADYDDDEEEEEEEEEEDEHADRVAVSGPGKGPILREGFYEV 446
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 28.9 bits (63), Expect = 1.8
Identities = 19/45 (42%), Positives = 25/45 (55%)
Frame = +1
Query: 284 AKSSSRRSASSESASRRSASSESAPFNLRSREPIYSNEFGNFFEI 328
+ SSS S+SS S S S+SS S+PF+ P G FFE+
Sbjct: 361 SSSSSSSSSSSSSWSSPSSSSSSSPFS-SGPAPAPGPPSGAFFEL 492
>AV411485
Length = 389
Score = 28.5 bits (62), Expect = 2.4
Identities = 15/52 (28%), Positives = 26/52 (49%)
Frame = -2
Query: 372 KGEFELVGQRNENQQEQREYEEDEQEEERSQQVQRYRARLTPGDVYVIPAGY 423
KG L R +N ++ EED+ ++E + + +YR R+ V + GY
Sbjct: 388 KGHSTLDYARWDNVEDDSSEEEDDDDDEDEESLPQYRFRVKTVGVRPVK*GY 233
>BP040798
Length = 521
Score = 28.5 bits (62), Expect = 2.4
Identities = 17/46 (36%), Positives = 27/46 (57%)
Frame = +1
Query: 90 LFENENGHIRLLQRFDKRSKIFENLQNYRLLEYHSKPHTLFLPQHN 135
L +NE+ H++ + KRS + +LQ + L+ SK H L +P HN
Sbjct: 304 LEDNEH*HLKQWLQKQKRSLLETSLQRVKPLQEASK-HWLQMPHHN 438
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 28.5 bits (62), Expect = 2.4
Identities = 17/65 (26%), Positives = 29/65 (44%)
Frame = +1
Query: 368 VEEGKGEFELVGQRNENQQEQREYEEDEQEEERSQQVQRYRARLTPGDVYVIPAGYPNVV 427
VEE E + E ++E+ E EE+E+EEE + + P + P G ++
Sbjct: 205 VEEEVEEEVEEEEEEEEEEEEGEEEEEEEEEEEEVEAEAEEEDDEPIQKLLEPMGKEQII 384
Query: 428 KASSD 432
+D
Sbjct: 385 SLLAD 399
>AI967315
Length = 1308
Score = 28.1 bits (61), Expect = 3.1
Identities = 17/49 (34%), Positives = 25/49 (50%)
Frame = -1
Query: 15 IATISKAINSTLSSQYSLISHHIKVKIIQIIMAIKAPFQLLMLLGIFFL 63
I T+S + TLS+ + H K +I I +K P L +L+ FFL
Sbjct: 1137 IVTVSVKLYLTLSTSSGVRKGH*K*TVIFIFQILKTPKFLSLLIFFFFL 991
>BP058107
Length = 496
Score = 27.7 bits (60), Expect = 4.1
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +3
Query: 272 VSREQIEELSRHAKSSSRRSASSESASRRSASSESAPFNLRSREPIYSNE 321
+SR + S S+S SAS+ SAS SAS N R + +NE
Sbjct: 165 LSRITLSTSSASTSSASTGSASTSSASTSSASRSCGSSNSRKGNSLGTNE 314
Score = 27.3 bits (59), Expect = 5.4
Identities = 21/60 (35%), Positives = 28/60 (46%)
Frame = +3
Query: 273 SREQIEELSRHAKSSSRRSASSESASRRSASSESAPFNLRSREPIYSNEFGNFFEITPEK 332
S + ++ S+S SAS+ SAS SAS+ SA + S N G E TP K
Sbjct: 153 STNTLSRITLSTSSASTSSASTGSASTSSASTSSASRSCGSSNSRKGNSLGT-NEGTPSK 329
>CN825310
Length = 747
Score = 27.7 bits (60), Expect = 4.1
Identities = 25/88 (28%), Positives = 40/88 (45%), Gaps = 5/88 (5%)
Frame = +1
Query: 225 ILEAAFNSNYEEIERVLIEEQEQEPRHRRGLRDRRHKQSQEANVIVKVSREQI-----EE 279
I + A + EE E EQ +P L+ +Q + +V + S+++ E
Sbjct: 430 IPQNALSDMEEESEVDDENEQSDQPYKDNALQIIVREQMDDVHVSPESSQKKEGNQVKES 609
Query: 280 LSRHAKSSSRRSASSESASRRSASSESA 307
S+H SS +S S+ S RRS S + A
Sbjct: 610 SSQHQVSSLTQSTSARSEGRRSRSGKKA 693
>TC14892 similar to UP|Q9Z0W6 (Q9Z0W6) Pax transcription activation domain
interacting protein PTIP, partial (3%)
Length = 1502
Score = 27.7 bits (60), Expect = 4.1
Identities = 10/28 (35%), Positives = 20/28 (70%)
Frame = +2
Query: 380 QRNENQQEQREYEEDEQEEERSQQVQRY 407
Q+ + QQ+++ E+D +EE+ + + QRY
Sbjct: 665 QQKQKQQQEQVEEQDSKEEQHTAKNQRY 748
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,376,410
Number of Sequences: 28460
Number of extensions: 77204
Number of successful extensions: 687
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 653
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 677
length of query: 456
length of database: 4,897,600
effective HSP length: 93
effective length of query: 363
effective length of database: 2,250,820
effective search space: 817047660
effective search space used: 817047660
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148289.3