BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.1 + phase: 0
(340 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14883 32 0.20
TC18971 similar to PIR|A88363|A88363 protein F26H11.1 [imported]... 30 0.76
BP033968 28 2.2
TC15212 homologue to UP|TBB7_MAIZE (Q41784) Tubulin beta-7 chain... 28 2.9
TC16402 similar to UP|RS7_AVIMR (Q9ZNS1) 40S ribosomal protein S... 28 2.9
BP050361 28 2.9
AV414419 27 4.9
TC13047 similar to UP|Q9SXF7 (Q9SXF7) Amino acid transporter-lik... 27 6.4
BI420625 27 6.4
TC12271 similar to UP|Q9FVD4 (Q9FVD4) Ser/Thr specific protein p... 26 8.4
>TC14883
Length = 1222
Score = 31.6 bits (70), Expect = 0.20
Identities = 19/65 (29%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +1
Query: 220 GCV----IGISWAFISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCG 275
GC+ + I+ A++ N NL L + +L+ K GTA S ++ + E ++ CG
Sbjct: 727 GCLGVWFMAINGAYLRNCPNLHLSRFL---LLLCQKCFRGTACSSSSLWSGVIEDLLWCG 897
Query: 276 PSLTV 280
P++T+
Sbjct: 898 PTVTI 912
>TC18971 similar to PIR|A88363|A88363 protein F26H11.1 [imported] -
Caenorhabditis elegans {Caenorhabditis elegans;} ,
partial (8%)
Length = 378
Score = 29.6 bits (65), Expect = 0.76
Identities = 19/74 (25%), Positives = 31/74 (41%)
Frame = +1
Query: 212 LIANPNSYGCVIGISWAFISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKV 271
L +NPN C + I+ W+LE P G+ I + S F+ Q +V
Sbjct: 13 LCSNPNPTQCNVSARKRIINKLWHLEFP----GTCCI*QR--------SRACFLCRQSRV 156
Query: 272 ISCGPSLTVFGLVL 285
C S ++F ++
Sbjct: 157 CICNASPSIFNFII 198
>BP033968
Length = 437
Score = 28.1 bits (61), Expect = 2.2
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +3
Query: 195 TSSRLPFLQLMKRVWLKLIANPN--SYGCVIGISWAFISNRW 234
TS LPF ++ K+ WL +I+NP + C +G IS W
Sbjct: 150 TS**LPFSKVGKQGWLFIISNPRFFLFHCRVGTHLIIISISW 275
>TC15212 homologue to UP|TBB7_MAIZE (Q41784) Tubulin beta-7 chain (Beta-7
tubulin), partial (30%)
Length = 659
Score = 27.7 bits (60), Expect = 2.9
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 93 SSKVSYSWSITSFSLCTLTNSL 114
S K+ Y W + SF LC++ N L
Sbjct: 425 SFKLQYEWVLASFKLCSIQNCL 490
>TC16402 similar to UP|RS7_AVIMR (Q9ZNS1) 40S ribosomal protein S7, partial
(97%)
Length = 1044
Score = 27.7 bits (60), Expect = 2.9
Identities = 15/43 (34%), Positives = 22/43 (50%), Gaps = 11/43 (25%)
Frame = +2
Query: 87 ALWAKCSSKVSY---------SWSITSFSLC--TLTNSLVVGI 118
A W +CS +VSY W++ + LC T++N L GI
Sbjct: 611 AYWERCSFRVSYYRSLKCCLVYWNVWRYRLCSHTISNLLTCGI 739
>BP050361
Length = 445
Score = 27.7 bits (60), Expect = 2.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +3
Query: 225 ISWAFISNRWNLELPSMVEGS 245
I W+FISNRW ++ S +E S
Sbjct: 213 IPWSFISNRWFNKILSSIEAS 275
>AV414419
Length = 396
Score = 26.9 bits (58), Expect = 4.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -3
Query: 48 YFTLPLFAFEFTAHIDPFKMNFLFI 72
+FT L F F H + FK NFL I
Sbjct: 76 HFTSSLLIFAFKNHTNKFKKNFLII 2
>TC13047 similar to UP|Q9SXF7 (Q9SXF7) Amino acid transporter-like protein
1, partial (7%)
Length = 416
Score = 26.6 bits (57), Expect = 6.4
Identities = 12/27 (44%), Positives = 19/27 (69%), Gaps = 1/27 (3%)
Frame = +3
Query: 89 WAKCSSKVSYS-WSITSFSLCTLTNSL 114
W+ SS+ S+S WS+TS S+C + + L
Sbjct: 189 WSSRSSRSSWSLWSLTSSSMCRMISIL 269
>BI420625
Length = 266
Score = 26.6 bits (57), Expect = 6.4
Identities = 13/57 (22%), Positives = 25/57 (43%)
Frame = -3
Query: 29 RWWKIFTREQCDAINKLVCYFTLPLFAFEFTAHIDPFKMNFLFIGADTLSKLIIVAV 85
+WW++F R C + L CY F ++ I ++ F L +I++ +
Sbjct: 246 QWWRLFPRHCCLSFIPLQCYLPQ*TFIILLSSPIRKDHLSLKF*HKRKLPPIILILI 76
>TC12271 similar to UP|Q9FVD4 (Q9FVD4) Ser/Thr specific protein
phosphatase 2A B regulatory subunit beta isoform,
partial (9%)
Length = 406
Score = 26.2 bits (56), Expect = 8.4
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +3
Query: 43 NKLVCYFTLPLFAF 56
N+L+C+F LP+F+F
Sbjct: 30 NRLLCHFNLPIFSF 71
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,174,927
Number of Sequences: 28460
Number of extensions: 85145
Number of successful extensions: 629
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 629
length of query: 340
length of database: 4,897,600
effective HSP length: 91
effective length of query: 249
effective length of database: 2,307,740
effective search space: 574627260
effective search space used: 574627260
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148289.1