BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147964.4 + phase: 0 /pseudo
(276 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17486 similar to UP|RU17_ARATH (Q42404) U1 small nuclear ribon... 318 6e-88
BP067368 71 2e-13
TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1... 60 3e-10
BP066300 55 1e-08
BI419822 55 1e-08
BP047377 55 2e-08
TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding p... 54 2e-08
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 54 3e-08
TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotei... 54 3e-08
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 51 2e-07
TC16457 similar to UP|Q41124 (Q41124) Chloroplast RNA binding pr... 50 4e-07
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 49 9e-07
BP036697 48 2e-06
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 48 2e-06
TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor ... 47 4e-06
TC14420 similar to UP|ROC3_NICSY (P19682) 28 kDa ribonucleoprote... 46 6e-06
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 45 1e-05
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 45 1e-05
TC12531 similar to UP|Q9ASQ8 (Q9ASQ8) At1g22910/F19G10_13, parti... 45 2e-05
TC10057 similar to UP|Q41124 (Q41124) Chloroplast RNA binding pr... 43 7e-05
>TC17486 similar to UP|RU17_ARATH (Q42404) U1 small nuclear
ribonucleoprotein 70 kDa (U1 snRNP 70 kDa) (snRNP70)
(U1-70K), partial (39%)
Length = 595
Score = 318 bits (815), Expect = 6e-88
Identities = 154/168 (91%), Positives = 161/168 (95%)
Frame = +3
Query: 3 DPLLRNQNAAVQARTKAQNRANVLQLKLIGQSHPTGLTANLLKLFEPRPPLEYKPPPEKR 62
DPLLRNQNAAVQAR KAQNRANVLQLKLIGQSHPTGLTANLLKLFEPRPPLEYKPPPEKR
Sbjct: 90 DPLLRNQNAAVQARAKAQNRANVLQLKLIGQSHPTGLTANLLKLFEPRPPLEYKPPPEKR 269
Query: 63 KCPPLTGMAQFVSKFAEPGEPEYSPPVPVVETPAERRARVHKLRLEKGAAKAAEELEKYD 122
KCPPL+GMAQFV KFAEPG+ EYSPP+PV ETPA+RR RVHKLRLEKGAAKAAEELEKYD
Sbjct: 270 KCPPLSGMAQFVGKFAEPGDAEYSPPIPVAETPAQRRERVHKLRLEKGAAKAAEELEKYD 449
Query: 123 PHNDPNISGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDA 170
P NDPN+SGDPYKTLFVA+LSYETTESRIKREFESYG IKRVRLVTD+
Sbjct: 450 PQNDPNVSGDPYKTLFVAKLSYETTESRIKREFESYGPIKRVRLVTDS 593
>BP067368
Length = 322
Score = 71.2 bits (173), Expect = 2e-13
Identities = 33/39 (84%), Positives = 36/39 (91%)
Frame = -2
Query: 238 VNQRHSGREQQQSGPSRSEEPRVREERHADRDREKSRER 276
VNQRHSGREQ +S PSRSEEPRVRE+R+ DRDREKSRER
Sbjct: 321 VNQRHSGREQHKSAPSRSEEPRVREDRNVDRDREKSRER 205
>TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1_4
{Arabidopsis thaliana;}, partial (55%)
Length = 1276
Score = 60.5 bits (145), Expect = 3e-10
Identities = 39/158 (24%), Positives = 75/158 (46%), Gaps = 10/158 (6%)
Frame = +2
Query: 127 PNISGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHT 186
P+I+ D Y +L V +++ TT + F+ YG + + + D + + RG+AF+ Y +
Sbjct: 113 PDIT-DTY-SLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYA 286
Query: 187 RDMKAAYKQADGRKIEGRRVLVD----------VERGRTVPNWRPRRLGGGLGTTRVGGD 236
+ A + DGR ++GR + V + +GR + + + G + R
Sbjct: 287 DEASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIESEERAKQGSRSRSPRRRHR 466
Query: 237 EVNQRHSGREQQQSGPSRSEEPRVREERHADRDREKSR 274
+ ++ R+ ++ SRS + R +RH RDR+ R
Sbjct: 467 DDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRR 580
>BP066300
Length = 420
Score = 55.5 bits (132), Expect = 1e-08
Identities = 25/74 (33%), Positives = 46/74 (61%)
Frame = -3
Query: 142 LSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQADGRKI 201
LS+ TTE ++ F+++G + V LV D +N+PRG+AF+ Y + + A + G+ +
Sbjct: 412 LSFRTTEESLRNCFKNFGQLVDVXLVMDKLANRPRGFAFLRYATEEESQKAIEGMHGKFL 233
Query: 202 EGRRVLVDVERGRT 215
+GR + V+V + R+
Sbjct: 232 DGRVIFVEVAKPRS 191
>BI419822
Length = 380
Score = 55.1 bits (131), Expect = 1e-08
Identities = 28/98 (28%), Positives = 50/98 (50%)
Frame = +2
Query: 138 FVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQAD 197
FV L++ T +++ F YG I +++ D E+ + RG+ F+ + + MK A + +
Sbjct: 86 FVGGLAWATDNEALEKAFSPYGEIVESKVINDRETGRSRGFGFVTFASEQAMKDAIEAMN 265
Query: 198 GRKIEGRRVLVDVERGRTVPNWRPRRLGGGLGTTRVGG 235
G+ ++GR + V+ + R R GGG G GG
Sbjct: 266 GQNLDGRNITVNEAQSRGSSGGGGRYGGGGGGYGGGGG 379
>BP047377
Length = 564
Score = 54.7 bits (130), Expect = 2e-08
Identities = 34/103 (33%), Positives = 50/103 (48%), Gaps = 1/103 (0%)
Frame = +3
Query: 130 SGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDM 189
S ++ +FV + Y+ TE ++ + G + RLV D E+ KP+GY F EY
Sbjct: 114 SQSQHRCVFVGNIPYDATEEQLIEICQEVGPVVSFRLVIDRETGKPKGYGFCEYKDEETA 293
Query: 190 KAAYKQADGRKIEGRRVLVDVERGRTVPNWRPRRLG-GGLGTT 231
+A + G +I GR++ VD N R R G GG G T
Sbjct: 294 LSARRNLQGYEINGRQLRVDFAE-NDKGNDRNREQGRGGPGMT 419
>TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding protein
PsGRBP, partial (88%)
Length = 683
Score = 54.3 bits (129), Expect = 2e-08
Identities = 30/108 (27%), Positives = 49/108 (44%)
Frame = +1
Query: 137 LFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQA 196
LF+ LSY + ++ F S+G + R++ D ++ + RG+ F+ + +A
Sbjct: 190 LFIGGLSYGVDDKSLEDAFSSFGTVAEARVIVDRDTGRSRGFGFVSFDSEESASSALSSM 369
Query: 197 DGRKIEGRRVLVDVERGRTVPNWRPRRLGGGLGTTRVGGDEVNQRHSG 244
DG+ + GR + V R R GGG GT G R+SG
Sbjct: 370 DGQDLNGRNIRVSYANDRPA----GPRGGGGYGT----GGGYGDRNSG 489
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 53.9 bits (128), Expect = 3e-08
Identities = 27/92 (29%), Positives = 49/92 (52%)
Frame = +1
Query: 138 FVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQAD 197
FV L++ T +++ F SYG I ++V D E+ + RG+ F+ + M++A + +
Sbjct: 91 FVGGLAWTTDSHTLEQAFSSYGEIIDSKVVNDRETGRSRGFGFVTFTSEEAMRSAIEGMN 270
Query: 198 GRKIEGRRVLVDVERGRTVPNWRPRRLGGGLG 229
G +++GR + V+ + R R GGG G
Sbjct: 271 GNELDGRNITVNEAQARGGGGGGGGRGGGGYG 366
>TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotein,
chloroplast precursor, partial (28%)
Length = 717
Score = 53.9 bits (128), Expect = 3e-08
Identities = 24/86 (27%), Positives = 45/86 (51%)
Frame = +3
Query: 130 SGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDM 189
S D +++V L + +R++ F +G ++ R+V D E+ + RG+ F+ D+
Sbjct: 63 SRDSGLSVYVGNLPWSVDTARLEEIFREHGNVENARIVMDRETGRSRGFGFVTMSSEADI 242
Query: 190 KAAYKQADGRKIEGRRVLVDVERGRT 215
A DG+ ++GR + V V GR+
Sbjct: 243 NGAIAALDGQSLDGRTIRVSVAEGRS 320
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 50.8 bits (120), Expect = 2e-07
Identities = 33/91 (36%), Positives = 47/91 (51%)
Frame = +1
Query: 137 LFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQA 196
L+V RLS T ++R F YG ++ V + D YAF+E+ RD A
Sbjct: 97 LYVGRLSSRTRSRDLERVFSRYGRVRDVDMKRD--------YAFVEFSDPRDADDARYNL 252
Query: 197 DGRKIEGRRVLVDVERGRTVPNWRPRRLGGG 227
DGR ++G R++V+ +G VP R R GGG
Sbjct: 253 DGRDVDGSRLIVEFAKG--VP--RGSREGGG 333
>TC16457 similar to UP|Q41124 (Q41124) Chloroplast RNA binding protein
precursor, partial (30%)
Length = 655
Score = 50.1 bits (118), Expect = 4e-07
Identities = 23/78 (29%), Positives = 41/78 (52%)
Frame = +2
Query: 137 LFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQA 196
LFV LS+ T + + F YG + R++ D E+ + RGY F+ Y +++ A
Sbjct: 20 LFVGNLSWTVTSESLIQVFPEYGTVVGARVLYDGETGRSRGYGFVCYSKRSELETALISL 199
Query: 197 DGRKIEGRRVLVDVERGR 214
+ ++EGR + V + G+
Sbjct: 200 NNVELEGRAIRVSLAEGK 253
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 48.9 bits (115), Expect = 9e-07
Identities = 41/147 (27%), Positives = 63/147 (41%), Gaps = 7/147 (4%)
Frame = +2
Query: 82 EPEYSPPVPVVETPAER------RARVHKLRLEKGAAKAAEELEKYDPHNDPNISGDP-Y 134
E E + + E P ER R ++H L +AAE+ + + DP +
Sbjct: 443 EEEEEEDLELEEEPVERLLEPFTREQLHSL-----VKQAAEKYPDFVENVRQLADVDPAH 607
Query: 135 KTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYK 194
+ +FV L ++TT + F YG I+ + VTD + K +GYAFI + H + A K
Sbjct: 608 RKIFVHGLGWDTTAETLTSVFSKYGEIEDCKAVTDKVTGKSKGYAFILFKHRDGCRRALK 787
Query: 195 QADGRKIEGRRVLVDVERGRTVPNWRP 221
+KI R + VP P
Sbjct: 788 NPQ-KKIGNRTTSSQLASAGPVPATPP 865
>BP036697
Length = 578
Score = 48.1 bits (113), Expect = 2e-06
Identities = 40/141 (28%), Positives = 65/141 (45%), Gaps = 2/141 (1%)
Frame = +3
Query: 122 DPHN--DPNISGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYA 179
+PH+ + +S +T++V L + E ++ F YG I + L +P GYA
Sbjct: 51 NPHSPKEKKMSRRTSRTVYVGNLPGDIREREVEDLFYKYGNITHIDLKVPP---RPPGYA 221
Query: 180 FIEYLHTRDMKAAYKQADGRKIEGRRVLVDVERGRTVPNWRPRRLGGGLGTTRVGGDEVN 239
F+E+ T+D + A + DG +G R+ V++ G G G ++R D +
Sbjct: 222 FVEFEDTQDAEDAIRGRDGYDFDGHRLRVELAHG-----------GRGNSSSR---DRYS 359
Query: 240 QRHSGREQQQSGPSRSEEPRV 260
SGR + G SR E RV
Sbjct: 360 SHSSGRGGR--GVSRRSEYRV 416
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 47.8 bits (112), Expect = 2e-06
Identities = 27/77 (35%), Positives = 41/77 (53%)
Frame = +2
Query: 137 LFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQA 196
L+V LS T E ++R F YG ++ V D + N +AF+E+ RD A
Sbjct: 107 LYVGHLSSRTRERDLERAFSKYGRVRGV----DMKHN----FAFVEFSDPRDADDARYNL 262
Query: 197 DGRKIEGRRVLVDVERG 213
DGR +EG R++V+ +G
Sbjct: 263 DGRDVEGSRIVVEFAKG 313
>TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor SCL30a, 30a
kD, partial (71%)
Length = 1073
Score = 47.0 bits (110), Expect = 4e-06
Identities = 36/143 (25%), Positives = 61/143 (42%), Gaps = 6/143 (4%)
Frame = +3
Query: 136 TLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQ 195
+L V L ++ ++R F +G +K + L D + +PRG+ F++++ D A
Sbjct: 171 SLLVRNLRHDCRPEDLRRPFGQFGPLKDIYLPKDYYTGQPRGFGFVQFVDPADAADAKYH 350
Query: 196 ADGRKIEGRRV-LVDVERGRTVPNWRPRRLGGGLGTTRVGGDEVNQRHSGR--EQQQSGP 252
DG+ + GR + +V E R P R +R GR ++++S P
Sbjct: 351 MDGQILLGRELTVVFAEENRKKPTEMRHR----------------ERSRGRSYDRRRSPP 482
Query: 253 SRSEEPRVREE---RHADRDREK 272
S PR RH R R +
Sbjct: 483 RYSRSPRYSRSPPPRHRSRSRSR 551
>TC14420 similar to UP|ROC3_NICSY (P19682) 28 kDa ribonucleoprotein,
chloroplast precursor (28RNP), partial (34%)
Length = 637
Score = 46.2 bits (108), Expect = 6e-06
Identities = 21/87 (24%), Positives = 43/87 (49%)
Frame = +1
Query: 128 NISGDPYKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTR 187
+ P ++V L ++ S++++ F +G + R+V D E+ + RG+ F+
Sbjct: 19 SFDAQPAFRVYVGNLPWDFDNSQLEQVFSEHGKVVSARVVYDRETGRSRGFGFVTMSDEA 198
Query: 188 DMKAAYKQADGRKIEGRRVLVDVERGR 214
++ A DG+ + GR + V+V R
Sbjct: 199 ELNDAIAALDGQSLGGRTIRVNVAEDR 279
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 45.4 bits (106), Expect = 1e-05
Identities = 27/116 (23%), Positives = 53/116 (45%)
Frame = +2
Query: 136 TLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQ 195
+LF ++ T++ ++K FE +G + ++V D S + RG+ F+ + + M A
Sbjct: 59 SLFYWWPAWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDA 238
Query: 196 ADGRKIEGRRVLVDVERGRTVPNWRPRRLGGGLGTTRVGGDEVNQRHSGREQQQSG 251
+G ++GR + VD + + G++R D R GR++ G
Sbjct: 239 MNGMDLDGRTITVDKAQPQ--------------GSSRDRDDGDRYRERGRDRDDRG 364
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 45.1 bits (105), Expect = 1e-05
Identities = 24/71 (33%), Positives = 40/71 (55%)
Frame = +1
Query: 134 YKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAY 193
++ +FV L ++TT + + F+ YGAI+ + VTD + K +GY FI + R + A
Sbjct: 463 HRKIFVHGLGWDTTATTLVYAFQQYGAIEDCKAVTDKVTGKSKGYGFILFKSRRGARNAL 642
Query: 194 KQADGRKIEGR 204
K+ +KI R
Sbjct: 643 KEPQ-KKIGNR 672
>TC12531 similar to UP|Q9ASQ8 (Q9ASQ8) At1g22910/F19G10_13, partial (39%)
Length = 688
Score = 44.7 bits (104), Expect = 2e-05
Identities = 34/122 (27%), Positives = 57/122 (45%), Gaps = 8/122 (6%)
Frame = -1
Query: 120 KYDPHNDPNISGDP-YKTLFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGY 178
K P N GD Y +FV L++ET + +K+ FE +G I ++TD + + +GY
Sbjct: 409 KMTPVNLAGQFGDTTYTKVFVGGLAWETQKETMKKYFEQFGDILEAVVITDKATGRSKGY 230
Query: 179 AFIEYLHTRDMKAAYKQA-------DGRKIEGRRVLVDVERGRTVPNWRPRRLGGGLGTT 231
F+ + R+ +AA + DGR+ + V+R + P++ GG
Sbjct: 229 GFVTF---REPEAAMRACVDAAPVIDGRRANCNLASLGVQRSKPS---TPKQHGGAGRNF 68
Query: 232 RV 233
RV
Sbjct: 67 RV 62
>TC10057 similar to UP|Q41124 (Q41124) Chloroplast RNA binding protein
precursor, partial (49%)
Length = 598
Score = 42.7 bits (99), Expect = 7e-05
Identities = 19/74 (25%), Positives = 37/74 (49%)
Frame = +2
Query: 137 LFVARLSYETTESRIKREFESYGAIKRVRLVTDAESNKPRGYAFIEYLHTRDMKAAYKQA 196
L+ L Y +++ E YG+ + + ++ D +S K RG+AF+ D +
Sbjct: 320 LYFGNLPYSVDSAQLAGLIEEYGSAELIEVLYDRDSGKSRGFAFVTMSCVEDCNTVIENL 499
Query: 197 DGRKIEGRRVLVDV 210
DG++ GR + V++
Sbjct: 500 DGKEFLGRTLRVNL 541
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,203,300
Number of Sequences: 28460
Number of extensions: 66221
Number of successful extensions: 645
Number of sequences better than 10.0: 138
Number of HSP's better than 10.0 without gapping: 560
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 615
length of query: 276
length of database: 4,897,600
effective HSP length: 89
effective length of query: 187
effective length of database: 2,364,660
effective search space: 442191420
effective search space used: 442191420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC147964.4