BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147961.2 - phase: 0 /pseudo
(451 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10799 weakly similar to UP|O23678 (O23678) CER1-like protein, ... 172 1e-43
TC15841 similar to UP|O80938 (O80938) CER1-like protein, partial... 163 5e-41
BP046732 73 8e-14
TC14868 similar to UP|Q8H1Z0 (Q8H1Z0) Cuticle protein, partial (... 67 5e-12
AU089312 52 2e-07
TC11584 similar to UP|O23679 (O23679) CER1 protein, partial (30%) 43 9e-05
TC19282 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomera... 32 0.21
TC18241 31 0.48
TC14672 homologue to UP|Q43106 (Q43106) H(+)-transporting ATPase... 28 2.4
TC17901 weakly similar to UP|Q94EI5 (Q94EI5) AT5g62130/mtg10_150... 28 2.4
TC13302 28 3.1
TC9818 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter, ... 28 3.1
BP043774 28 3.1
TC15651 weakly similar to UP|Q9FZF0 (Q9FZF0) T2E6.19, partial (41%) 28 4.0
TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Ar... 28 4.0
BP064703 27 5.3
TC14904 weakly similar to UP|Q7XBC0 (Q7XBC0) ThiJ-like protein, ... 27 5.3
AV767518 27 6.9
TC10177 weakly similar to UP|Q9S728 (Q9S728) EN/SPM-like transpo... 27 6.9
TC9810 similar to UP|HEM3_PEA (Q43082) Porphobilinogen deaminase... 27 6.9
>TC10799 weakly similar to UP|O23678 (O23678) CER1-like protein, partial
(13%)
Length = 605
Score = 172 bits (436), Expect = 1e-43
Identities = 75/106 (70%), Positives = 90/106 (84%)
Frame = +2
Query: 342 VGDEWDEYEQMEAPKGSLFIPFSHFPPKKMRKDCFYHYTPAMITPTTFMNSHSCENWLPR 401
VGD+ +E E+ +A GSLFIPFS FPPKK+RKDCFY TPAM+TP + +N HSCENWLPR
Sbjct: 2 VGDQLNEAERQKAAHGSLFIPFSRFPPKKLRKDCFYLSTPAMVTPPSLVNVHSCENWLPR 181
Query: 402 RVMSAWRIAGIIHALEGWNVHECGDTILSTEKVWEASIRHGFQPLK 447
RVMSAWRIAGI+HALE WN++ECGD + S EKVW+AS+ HGF+PLK
Sbjct: 182 RVMSAWRIAGILHALEDWNINECGDVMFSIEKVWQASLYHGFRPLK 319
>TC15841 similar to UP|O80938 (O80938) CER1-like protein, partial (14%)
Length = 636
Score = 163 bits (413), Expect = 5e-41
Identities = 70/108 (64%), Positives = 88/108 (80%)
Frame = +1
Query: 339 IWLVGDEWDEYEQMEAPKGSLFIPFSHFPPKKMRKDCFYHYTPAMITPTTFMNSHSCENW 398
IWLVGD E EQ+ AP G++FIP+S FPPKK RKDC YH TPAM P++ N HSCE+W
Sbjct: 19 IWLVGDGLTEEEQVNAPXGTIFIPYSQFPPKKYRKDCSYHCTPAMQIPSSVENIHSCEDW 198
Query: 399 LPRRVMSAWRIAGIIHALEGWNVHECGDTILSTEKVWEASIRHGFQPL 446
LPRRVMSAWRIAGI+H+LEGW HECG T+ + +KVW+++++HGF+PL
Sbjct: 199 LPRRVMSAWRIAGIVHSLEGWTEHECGYTMHNIDKVWKSTLQHGFKPL 342
>BP046732
Length = 495
Score = 73.2 bits (178), Expect = 8e-14
Identities = 41/117 (35%), Positives = 61/117 (52%), Gaps = 1/117 (0%)
Frame = -1
Query: 336 TPKIWLVGDEWDEYEQMEAPKGSLFIPFSHFPPKKMRKDCFYHYTPAMITPTTFMNSHSC 395
T K W+VG EQ AP+G+ F F P R++C Y AM P C
Sbjct: 426 TCKTWIVGKWITPREQSWAPRGTHFHQFVVPPILSFRRNCTYGGLAAMRLPQDVEGLGCC 247
Query: 396 ENWLPRRVMSAWRIAGIIHALEGWNVHECGD-TILSTEKVWEASIRHGFQPLKNILT 451
E + R V+ A G++H+LEGW+ HE G + + VW+A+++HG +P+ + LT
Sbjct: 246 EYTMERGVVHACHAGGVVHSLEGWSHHEVGAIDVNRIDLVWKAALKHGLRPVSS*LT 76
>TC14868 similar to UP|Q8H1Z0 (Q8H1Z0) Cuticle protein, partial (16%)
Length = 570
Score = 67.4 bits (163), Expect = 5e-12
Identities = 36/100 (36%), Positives = 53/100 (53%), Gaps = 1/100 (1%)
Frame = +3
Query: 350 EQMEAPKGSLFIPFSHFPPKKMRKDCFYHYTPAMITPTTFMNSHSCENWLPRRVMSAWRI 409
EQ AP+G+ F F P R+DC Y AM P SCE + R V+ A
Sbjct: 15 EQSWAPRGTHFHQFVVPPILPFRRDCTYGELAAMRLPEDVEGLGSCEYTMERGVVHACHA 194
Query: 410 AGIIHALEGWNVHECGD-TILSTEKVWEASIRHGFQPLKN 448
G++H+LEGW HE G + + VW+A+++HG +P+ +
Sbjct: 195 GGVVHSLEGWTHHEVGAIDVDRIDVVWKAALKHGLRPVSS 314
>AU089312
Length = 272
Score = 52.4 bits (124), Expect = 2e-07
Identities = 22/31 (70%), Positives = 24/31 (76%)
Frame = +3
Query: 79 FHSLHHTKFRRNYSLFMPMYDYIYGTVDKST 109
FHSLHHT F NYSLFMP Y YIY T DK++
Sbjct: 180 FHSLHHTXFXTNYSLFMPXYXYIYXTXDKAS 272
>TC11584 similar to UP|O23679 (O23679) CER1 protein, partial (30%)
Length = 617
Score = 43.1 bits (100), Expect = 9e-05
Identities = 27/69 (39%), Positives = 39/69 (56%)
Frame = +3
Query: 1 G*PNFVHCTVILHRIHDISYGF*LTMVENRWCDSYSNIACWTSRVFILLAS*STSSSLSI 60
G*PN V +L + ++ G + +ENRW + + +C S V +LLAS STS L +
Sbjct: 330 G*PNIV*WVAVLLGMLYLARGVTSSTLENRWGVNSNIASCRPSGVSLLLASQSTSPPLPL 509
Query: 61 LSLSFSPSF 69
L + FSP F
Sbjct: 510 LKIPFSPPF 536
>TC19282 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomerase
(Fragment), partial (17%)
Length = 520
Score = 32.0 bits (71), Expect = 0.21
Identities = 21/75 (28%), Positives = 36/75 (48%)
Frame = -2
Query: 121 KESPDVVHLTHLTTFNSIYQLRLGFASLASNPQTSKWYLHLMWPFTMFSMLMTWICGRAF 180
K S ++H+ T +++ +R F+S ++ W+L L W +MF + RA
Sbjct: 309 KSSQFILHIAIATL--ALFGIRAPFSSRILGFRS*GWFLELEWN*SMFLEELVKSYHRAI 136
Query: 181 VLESNSFKNLKLQCW 195
+ SN K L + CW
Sbjct: 135 CVNSNRIKTL-VSCW 94
>TC18241
Length = 574
Score = 30.8 bits (68), Expect = 0.48
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = +1
Query: 67 PSFFNCH*ACHLFHSLHHTKFRRNYSLFMPM 97
PS CH HL H HH+ + YSL +P+
Sbjct: 163 PSCHRCHPQQHLHHQQHHSIKQTKYSLNLPL 255
>TC14672 homologue to UP|Q43106 (Q43106) H(+)-transporting ATPase , partial
(14%)
Length = 828
Score = 28.5 bits (62), Expect = 2.4
Identities = 15/27 (55%), Positives = 20/27 (73%), Gaps = 2/27 (7%)
Frame = +3
Query: 78 LFHSLHHTKFRRNYSLFMP--MYDYIY 102
LFHSL+ + +RR+YSL +P M D IY
Sbjct: 546 LFHSLYVSSWRRHYSL*VPNNMADRIY 626
>TC17901 weakly similar to UP|Q94EI5 (Q94EI5) AT5g62130/mtg10_150, partial
(52%)
Length = 763
Score = 28.5 bits (62), Expect = 2.4
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -1
Query: 152 PQTSKWYLHLMWPFTMFSMLMTWICGRAFVLESNSFKNL 190
P +W+LH+ W MFS+ + +A + ++SF L
Sbjct: 394 PLQIEWFLHVPWTIKMFSI*WKFTVWKATLCNTSSFLTL 278
>TC13302
Length = 533
Score = 28.1 bits (61), Expect = 3.1
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 164 PFTMFSMLMTWICGRAFVLESNSFKNLKLQCWLIPRFKRQ 203
PF +F +CG L S+ + ++L CW+IP R+
Sbjct: 112 PFHVFHRTSGRVCG----LPSDKLEFIELDCWIIPCLPRE 219
>TC9818 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter, partial
(62%)
Length = 1162
Score = 28.1 bits (61), Expect = 3.1
Identities = 11/27 (40%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = -2
Query: 387 TTFMNSHSC-ENWLPRRVMSAWRIAGI 412
TT +C + W+PR ++ WRIAG+
Sbjct: 1068 TTASMPEACWKKWMPRAAITMWRIAGV 988
>BP043774
Length = 439
Score = 28.1 bits (61), Expect = 3.1
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +2
Query: 366 FPPKKMRKDCFYHYTPAMITPTTFMNSHSCENWLPRRVMSA 406
FPP+ +R F T + P+ FMN H LPR ++ A
Sbjct: 278 FPPRGLRS--FAAATRTSLPPSAFMNRHHRHRPLPRPLLPA 394
>TC15651 weakly similar to UP|Q9FZF0 (Q9FZF0) T2E6.19, partial (41%)
Length = 1055
Score = 27.7 bits (60), Expect = 4.0
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -3
Query: 331 LSPFNTPKIWLVGDEWDEYEQMEAPKGSLFIPFSHF 366
L + P I L+ +W Y + E + +L PFSHF
Sbjct: 495 LRTISFPVIQLLQRQWILYSREELLRSTLVFPFSHF 388
>TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Arabidopsis
thaliana;} , partial (19%)
Length = 765
Score = 27.7 bits (60), Expect = 4.0
Identities = 19/64 (29%), Positives = 32/64 (49%), Gaps = 2/64 (3%)
Frame = +2
Query: 312 KDELKELEQRINTSKGNLALSPFN-TPKIWLVGDE-WDEYEQMEAPKGSLFIPFSHFPPK 369
KDE+ + + IN+ + LSP N PK + + DE +E E + + S + P
Sbjct: 71 KDEVDSVYKGINSFRQTKNLSPLNRVPKAYCLADEIAEEMEDIPCEQVSQYYPGGSMQIP 250
Query: 370 KMRK 373
K++K
Sbjct: 251 KLQK 262
>BP064703
Length = 490
Score = 27.3 bits (59), Expect = 5.3
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Frame = +1
Query: 272 VLNNIPKGTNQVLLRGK-----FNKVAFVIANALCKKNVQVVVL 310
+L N P G + LRGK K A ++AN+ C N+++++L
Sbjct: 265 LLTNSPXGKQVLCLRGKRRYRHGKKTATLVANSACCVNLRMLLL 396
>TC14904 weakly similar to UP|Q7XBC0 (Q7XBC0) ThiJ-like protein, partial
(35%)
Length = 705
Score = 27.3 bits (59), Expect = 5.3
Identities = 21/68 (30%), Positives = 31/68 (44%), Gaps = 7/68 (10%)
Frame = -3
Query: 190 LKLQCWLIPRFKRQYFSKWQ------SKTFNNLIEEAIVEAELNGAKVISLGLFNKNH-Q 242
LK Q W +FK YFS+W+ S F+ E+ + + E+N LF H +
Sbjct: 703 LKSQFWF--KFKIHYFSRWKQMAPKLSTMFSTAYEKQLDQCEINN----HFSLFYTTHSR 542
Query: 243 LNERHEHY 250
N R+ Y
Sbjct: 541 FNIRNYSY 518
>AV767518
Length = 474
Score = 26.9 bits (58), Expect = 6.9
Identities = 11/42 (26%), Positives = 19/42 (45%)
Frame = -3
Query: 239 KNHQLNERHEHYIGRLPQLKIKVVDGSSLAAATVLNNIPKGT 280
+ ++ H G+LP + + G +LN +PKGT
Sbjct: 340 EKEEITAGHGRVTGKLPSPPVMRIRGGKYRPIMILNMLPKGT 215
>TC10177 weakly similar to UP|Q9S728 (Q9S728) EN/SPM-like transposon
protein, partial (38%)
Length = 578
Score = 26.9 bits (58), Expect = 6.9
Identities = 10/29 (34%), Positives = 16/29 (54%)
Frame = -3
Query: 169 SMLMTWICGRAFVLESNSFKNLKLQCWLI 197
++L W C A + E + NL L CW++
Sbjct: 390 AILKRWACVVAALFELSDATNLSLTCWVV 304
>TC9810 similar to UP|HEM3_PEA (Q43082) Porphobilinogen deaminase,
chloroplast precursor (PBG) (Hydroxymethylbilane
synthase) (HMBS) (Pre-uroporphyrinogen synthase) ,
partial (44%)
Length = 884
Score = 26.9 bits (58), Expect = 6.9
Identities = 15/37 (40%), Positives = 20/37 (53%), Gaps = 1/37 (2%)
Frame = +2
Query: 381 PAMITPTTFMNSHSCENWL-PRRVMSAWRIAGIIHAL 416
PAM+ P T E+WL PR + WR G++H L
Sbjct: 305 PAML-PETKTEIAYLEDWLLPRMELVCWRHLGLVHML 412
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.139 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,596,032
Number of Sequences: 28460
Number of extensions: 157283
Number of successful extensions: 1103
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1094
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1101
length of query: 451
length of database: 4,897,600
effective HSP length: 93
effective length of query: 358
effective length of database: 2,250,820
effective search space: 805793560
effective search space used: 805793560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC147961.2