BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147875.3 + phase: 0
(293 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089059 49 1e-06
TC16650 39 0.001
BP056411 33 0.074
TC17480 similar to UP|Q9FFY6 (Q9FFY6) Similarity to nascent poly... 32 0.13
TC12014 weakly similar to GB|AAM51584.1|21436013|AY116950 At1g10... 32 0.17
TC8613 similar to GB|AAC64889.1|3776572|T22H22 ESTs gb|R65052, g... 30 0.63
AV777016 29 1.1
BP060250 28 1.4
BF177810 28 1.4
TC14409 similar to PIR|A84668|A84668 Argonaute (AGO1)-like prote... 28 1.8
TC13749 similar to UP|Q9FKW9 (Q9FKW9) Alpha-mannosidase, partial... 28 2.4
TC14673 similar to UP|Q9LH76 (Q9LH76) Genomic DNA, chromosome 3,... 28 2.4
TC17494 weakly similar to UP|Q940M0 (Q940M0) At1g63980/F22C12_9,... 28 2.4
BP038568 27 3.1
TC12007 similar to UP|Q8W1A0 (Q8W1A0) Cysteine synthase, partial... 27 3.1
AV412250 27 3.1
TC13251 similar to UP|Q9AVK8 (Q9AVK8) Allergen Gly m Bd 28K (Fra... 27 3.1
AV777759 27 4.1
BP078616 27 4.1
TC15657 similar to UP|Q40977 (Q40977) Monodehydroascorbate reduc... 27 4.1
>AU089059
Length = 290
Score = 48.9 bits (115), Expect = 1e-06
Identities = 19/40 (47%), Positives = 30/40 (74%), Gaps = 1/40 (2%)
Frame = +2
Query: 42 CANLKNL-RYPFWGSSRPQYCGHPSFELQCKDELASITIM 80
C L NL +YPFW + +P++CGHPSF+L C+ +L ++ I+
Sbjct: 113 CRPLPNLIKYPFWTAEQPKHCGHPSFQLNCQ*DLVTMVII 232
>TC16650
Length = 538
Score = 38.9 bits (89), Expect = 0.001
Identities = 16/45 (35%), Positives = 23/45 (50%)
Frame = +1
Query: 219 GFGVEWNANNSLCHECQSSGGHCGYESSSREFTCFCKDGSFPHSC 263
G + W A++ C C S+GG CG++S F C+C D C
Sbjct: 160 GLLLNWTASD--CGVCNSTGGRCGFDSEIYTFRCYCADRVHAKHC 288
>BP056411
Length = 472
Score = 32.7 bits (73), Expect = 0.074
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = -3
Query: 86 ILEVSDSDHRLKVVRTDYWNNVCPTSLKNTTLDCTFFDYGSDSHNLTLYYD 136
++E+ ++ +R +V T W + CP +L +DC D N L++D
Sbjct: 413 LMEMRENGNRYGIVET--WKSCCPNTLDGDDMDCHAHDEPCPDRNTHLFFD 267
>TC17480 similar to UP|Q9FFY6 (Q9FFY6) Similarity to nascent polypeptide
associated complex alpha chain, partial (11%)
Length = 605
Score = 32.0 bits (71), Expect = 0.13
Identities = 16/46 (34%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -3
Query: 112 LKNTTLDCTFFDY-GSDSHNLTLYYDCPHTPFLMPDSFSPQFNCSI 156
++N +L + F++ S S L CPHTP ++ +SP NC I
Sbjct: 156 IRNLSLHLSSFNFCSSSSLKELLPRQCPHTPQVLIKRYSPNLNCLI 19
>TC12014 weakly similar to GB|AAM51584.1|21436013|AY116950
At1g10380/F14N23_32 {Arabidopsis thaliana;}, partial
(20%)
Length = 473
Score = 31.6 bits (70), Expect = 0.17
Identities = 11/27 (40%), Positives = 13/27 (47%)
Frame = +1
Query: 231 CHECQSSGGHCGYESSSREFTCFCKDG 257
C C+ S G CGY F C C +G
Sbjct: 76 CAACERSNGFCGYSGPYNSFICNCPNG 156
>TC8613 similar to GB|AAC64889.1|3776572|T22H22 ESTs gb|R65052,
gb|AA712146, gb|H76533, gb|H76282, gb|AA650771,
gb|H76287, gb|AA650887, gb|N37383, gb|Z29721 and
gb|Z29722 come from this gene. {Arabidopsis thaliana;},
partial (68%)
Length = 939
Score = 29.6 bits (65), Expect = 0.63
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 13/51 (25%)
Frame = -3
Query: 98 VVRTDYWNNVCPTSLKN----------TTLDCTFFDYGSDSHN---LTLYY 135
++R+ YW +C + +KN T D +FF G +HN +TL+Y
Sbjct: 724 LIRSQYWKVLCDSGIKNIFSHCLDKSRATGDRSFFLTGDKNHNTLVITLFY 572
>AV777016
Length = 625
Score = 28.9 bits (63), Expect = 1.1
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 260 PHSCSSGEHHCRFHCCVMFLHSIMSFH 286
P CS +H C H CV+ LH+ + F+
Sbjct: 276 PTQCSVNDHFC--HTCVLVLHAAVDFY 350
>BP060250
Length = 487
Score = 28.5 bits (62), Expect = 1.4
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 13/64 (20%)
Frame = +2
Query: 238 GGHCGYESSSREFTCFCKDGSFP-----------HSCSSGEH--HCRFHCCVMFLHSIMS 284
G H Y + S+ FC+ FP HS + H C FHC +M L+ ++
Sbjct: 164 GQHNIYSAGSQTHNIFCQTNYFPSLRFMENLPKAHSWHTKIHLPFCTFHCLLMQLNQLV- 340
Query: 285 FHLG 288
H+G
Sbjct: 341 -HIG 349
>BF177810
Length = 431
Score = 28.5 bits (62), Expect = 1.4
Identities = 17/44 (38%), Positives = 18/44 (40%)
Frame = -2
Query: 227 NNSLCHECQSSGGHCGYESSSREFTCFCKDGSFPHSCSSGEHHC 270
N+S H C H Y S R C F C SGEHHC
Sbjct: 322 NHSHSHCCCCC*KHFQYYHSDRL*KEGCCC*EFQRCCCSGEHHC 191
>TC14409 similar to PIR|A84668|A84668 Argonaute (AGO1)-like protein
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (9%)
Length = 674
Score = 28.1 bits (61), Expect = 1.8
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +1
Query: 263 CSSGEHHCRFHCCVMFL 279
C S EHHC F CC L
Sbjct: 52 CVSEEHHCNFCCCSYML 102
>TC13749 similar to UP|Q9FKW9 (Q9FKW9) Alpha-mannosidase, partial (17%)
Length = 618
Score = 27.7 bits (60), Expect = 2.4
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = +3
Query: 233 ECQSSGGHCGYESSSREFTCFCKDGSFP 260
EC GG C S++ C C+DG FP
Sbjct: 234 ECAGFGG-CISSERSKQKVCVCRDGIFP 314
>TC14673 similar to UP|Q9LH76 (Q9LH76) Genomic DNA, chromosome 3, BAC clone:
T21E2 (AT3g14790/T21E2_4), partial (32%)
Length = 1048
Score = 27.7 bits (60), Expect = 2.4
Identities = 13/46 (28%), Positives = 17/46 (36%)
Frame = -2
Query: 231 CHECQSSGGHCGYESSSREFTCFCKDGSFPHSCSSGEHHCRFHCCV 276
C+ C S G CG + F S P E H + CC+
Sbjct: 474 CNPCTSQGSRCGSPLQGL*SSRFPSGSSLPSQLKLAEAHPK*SCCL 337
>TC17494 weakly similar to UP|Q940M0 (Q940M0) At1g63980/F22C12_9, partial
(18%)
Length = 634
Score = 27.7 bits (60), Expect = 2.4
Identities = 21/49 (42%), Positives = 26/49 (52%)
Frame = -2
Query: 245 SSSREFTCFCKDGSFPHSCSSGEHHCRFHCCVMFLHSIMSFHLGSSSSS 293
SS R +CF SF S SSG CC +FLH+ SF L S++ S
Sbjct: 228 SSIRTLSCF----SF--SDSSGT------CCKIFLHNFFSFTLDSTTFS 118
>BP038568
Length = 553
Score = 27.3 bits (59), Expect = 3.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -1
Query: 109 PTSLKNTTLDCTFFDYGSDSHNLTLYYDCPHTPFL 143
P SL++ L FF + SH L + CP PFL
Sbjct: 211 PFSLQS*VLAGLFFSLPAPSHILPVSSPCPQAPFL 107
>TC12007 similar to UP|Q8W1A0 (Q8W1A0) Cysteine synthase, partial (34%)
Length = 592
Score = 27.3 bits (59), Expect = 3.1
Identities = 18/44 (40%), Positives = 21/44 (46%), Gaps = 8/44 (18%)
Frame = +2
Query: 240 HCGYESSSREFTCFCKDGSFPHSCSSGEHH-------CR-FHCC 275
+C S R FT DG+F SCSS HH CR +CC
Sbjct: 128 NC*DASLERRFT----DGNFIRSCSSSSHHIGEETRKCRQANCC 247
>AV412250
Length = 403
Score = 27.3 bits (59), Expect = 3.1
Identities = 17/43 (39%), Positives = 22/43 (50%), Gaps = 8/43 (18%)
Frame = +2
Query: 253 FCKDGSFPHSC----SSGEHHCRFHCC----VMFLHSIMSFHL 287
FC+ F C ++G+HH HC LHSI+SFHL
Sbjct: 47 FCRKQHFV*DCYL*RNAGQHH---HCLPSNHFSRLHSILSFHL 166
>TC13251 similar to UP|Q9AVK8 (Q9AVK8) Allergen Gly m Bd 28K (Fragment),
partial (11%)
Length = 484
Score = 27.3 bits (59), Expect = 3.1
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
Frame = -2
Query: 264 SSGEHHCRFHCCVMF--LHSIMSFHLG 288
SS HC F CV+F +I+ FHLG
Sbjct: 204 SSHYQHCHFEYCVIF*SKSNIIQFHLG 124
>AV777759
Length = 543
Score = 26.9 bits (58), Expect = 4.1
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -1
Query: 227 NNSLCHECQSSGGHCGYESSSREFTCFCKDGSF 259
NN+ + SS H SSS FT +C+D SF
Sbjct: 117 NNTQTNYSFSSFSHILISSSSFMFTLYCQDNSF 19
>BP078616
Length = 466
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -3
Query: 20 KIPTSSCADNLQQYNKCSSLFDCANLKNLRYPFWGSSRPQYC 61
K+ T C D+ + N C+ + + ++K+ R+PF P +C
Sbjct: 323 KVETFVCKDDQPRKNFCNKVKEPHSMKSFRHPFPLLRVPLHC 198
>TC15657 similar to UP|Q40977 (Q40977) Monodehydroascorbate reductase ,
partial (48%)
Length = 915
Score = 26.9 bits (58), Expect = 4.1
Identities = 13/38 (34%), Positives = 16/38 (41%), Gaps = 4/38 (10%)
Frame = +1
Query: 219 GFGVEWNA--NNSLCHECQ--SSGGHCGYESSSREFTC 252
G WN+ C C+ +SGG CG R F C
Sbjct: 523 GLSGGWNS*GEQGYCQSCKGPASGGECGSTRKGRSFLC 636
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.133 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,283,549
Number of Sequences: 28460
Number of extensions: 131982
Number of successful extensions: 830
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 830
length of query: 293
length of database: 4,897,600
effective HSP length: 90
effective length of query: 203
effective length of database: 2,336,200
effective search space: 474248600
effective search space used: 474248600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC147875.3