BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147714.8 + phase: 0 /pseudo
(2334 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 61 2e-09
TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycop... 35 0.17
BP071106 32 0.87
AV427333 32 0.87
BG662122 32 1.1
TC16198 32 1.1
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 32 1.1
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 32 1.1
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 32 1.5
AV408190 32 1.5
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 32 1.5
TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL ... 31 1.9
BP029871 31 1.9
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 31 1.9
TC18087 similar to UP|Q9LF46 (Q9LF46) 2-hydroxyphytanoyl-CoA lya... 31 2.5
AU251550 31 2.5
AV409168 27 4.0
TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.3... 30 4.3
AV769784 30 4.3
AV409036 30 5.6
>BP065719
Length = 567
Score = 61.2 bits (147), Expect = 2e-09
Identities = 41/175 (23%), Positives = 85/175 (48%), Gaps = 2/175 (1%)
Frame = +3
Query: 125 QHGSIPVTKTMEEMMEELAKELRHEIKANQGNADSFKTQDLCLVSKVDVPKKFKIPDFDR 184
+H + + + ++E+L + H N F + + V + ++P+ +K+P F +
Sbjct: 24 RHQNAGGQQNVAAVVEQLLNQ--HGFNVGFANRPHFVSAFIEEVLESELPRGWKVPKFTK 197
Query: 185 YNGLTCPQN--HIIKYVRKMGNYKDNDSLMIHCFQDSLMEDAAEWYTSLSKNDIHTFDEL 242
++G + HI +Y + G+ N++L + F SL ++A W+T+L+ +HT+ +L
Sbjct: 198 FSGDSGESTVEHIARYQIEAGDLAINENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWAQL 377
Query: 243 AAAFKSHYGFNIRLKPNREFLRSLS*KKEESFREYAQRWKGAAARITPALDEEEM 297
F + F K + + L S+ K ES +Y R++ +R + E E+
Sbjct: 378 ERIFHEQF-FRGECKVSXKDLASVKRKPAESIDDYLNRFRMLKSRCFTHVSEHEL 539
>TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (57%)
Length = 941
Score = 34.7 bits (78), Expect = 0.17
Identities = 25/96 (26%), Positives = 34/96 (35%), Gaps = 1/96 (1%)
Frame = +1
Query: 390 PPSYPYVSYSQHPFFPPFYHQYPLPPG-QPQVPVYAIAQQMKQQLPVQQQQQNQQARPTF 448
PP P Y + P PP+Y++ P PP P P Y + P P+
Sbjct: 31 PPPVPKPYYYKSPPPPPYYYKSPPPPSPSPPPPYYYQSPPPPSHSPPPPYYYKSPPPPSP 210
Query: 449 PPIPMLYAELLPTLLHRGHCTTRQGKPAPDPLPPRF 484
P P Y + P P+P P PP +
Sbjct: 211 SPPPPYYYKSPP-------------PPSPSPPPPYY 279
>BP071106
Length = 402
Score = 32.3 bits (72), Expect = 0.87
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +3
Query: 390 PPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVPVYA 424
P +PY+ QHP PP+ P P P VYA
Sbjct: 276 PAIHPYIWGGQHPLIPPYGTPVPYPAMYPPGAVYA 380
>AV427333
Length = 387
Score = 32.3 bits (72), Expect = 0.87
Identities = 28/99 (28%), Positives = 34/99 (34%), Gaps = 6/99 (6%)
Frame = +3
Query: 390 PPSYPY--VSYSQHPFFPP----FYHQYPLPPGQPQVPVYAIAQQMKQQLPVQQQQQNQQ 443
PPS+PY + HPF PP Y + P PP P P
Sbjct: 87 PPSHPYNPPTPPSHPFNPPPTPSHYFKSPPPPSHSFAP-----PPRGHPSPPPSSPPPPS 251
Query: 444 ARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPAPDPLPP 482
+ P PP P + P H +P P PLPP
Sbjct: 252 SHPFTPPPPHVRPPPSP---HHPITPPPHVRPPPPPLPP 359
>BG662122
Length = 386
Score = 32.0 bits (71), Expect = 1.1
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +2
Query: 473 GKPAPDPLPPRFRSDLK--CDFHQGALGHDVEGCYALKYIVKKLIDQGKL 520
GK PPR D C++ + + HD++ C+ LK ++KLI G+L
Sbjct: 155 GKSGQS*PPPRRGIDTTK*CEYRRSVV-HDIDDCFTLKREIEKLIKMGRL 301
>TC16198
Length = 572
Score = 32.0 bits (71), Expect = 1.1
Identities = 19/83 (22%), Positives = 40/83 (47%), Gaps = 1/83 (1%)
Frame = +2
Query: 378 MATVAPINAAQMPPSYPYVSYSQHPFFPPF-YHQYPLPPGQPQVPVYAIAQQMKQQLPVQ 436
++ + + +M + VS + P FPP +H +P + A Q + + L ++
Sbjct: 149 LSLILQSSLVEMAMAISSVSVALRPHFPPSPFHSRRYSFAKP-LRCLATKQNLPETLEIE 325
Query: 437 QQQQNQQARPTFPPIPMLYAELL 459
+ +++ +F P+P+LYA L
Sbjct: 326 SRPKSETVLHSFSPLPLLYAAAL 394
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 32.0 bits (71), Expect = 1.1
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +2
Query: 418 PQVPVYAIAQQMKQQLPVQQQQQNQQARP--TFPPIPMLYAELLP 460
PQV ++Q +QQLP QQQQ Q +P P + L + LP
Sbjct: 92 PQVQQQQLSQMQQQQLPQLQQQQLSQLQPQQQLPQLQQLQHQQLP 226
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 32.0 bits (71), Expect = 1.1
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = +3
Query: 401 HPFFPPFYHQYPLPPGQPQVPVYAIAQQMKQQLPVQQQQQNQQARPTFPPI-PMLYAELL 459
H PP PLP + Q+ ++ +A+ Q+ P +++ + +P PP+ P L+
Sbjct: 75 HHHHPPHTFTCPLPNPRQQLQIHRLARHPAQRRPPSPRRRRLRPQPLHPPLHPRPRHSLV 254
Query: 460 PTLL--HRGHCTTRQ 472
L HR H RQ
Sbjct: 255 RPRLGPHRLHLRRRQ 299
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 31.6 bits (70), Expect = 1.5
Identities = 25/87 (28%), Positives = 31/87 (34%)
Frame = +1
Query: 382 APINAAQMPPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVPVYAIAQQMKQQLPVQQQQQN 441
AP + P YP + Y QH F+PP P PP P P A P +
Sbjct: 172 APPYGMEFSP-YPSLPYPQHQFYPPPMAPPPPPPPAPAPP----APPPYWAAPPPRPYGP 336
Query: 442 QQARPTFPPIPMLYAELLPTLLHRGHC 468
T PP P A + + HC
Sbjct: 337 PLFTSTLPPRPPKPAPPKNVVKEKSHC 417
>AV408190
Length = 382
Score = 31.6 bits (70), Expect = 1.5
Identities = 30/99 (30%), Positives = 41/99 (41%), Gaps = 11/99 (11%)
Frame = +1
Query: 384 INAAQMPPSYPYVSYSQHPFFPPFYHQYPLPPGQ---------PQV--PVYAIAQQMKQQ 432
+ A+ PPS +H ++H PLPP Q PQV P + ++
Sbjct: 100 LQQAEAPPSPSKPLLRRH-----YFHLLPLPPWQDRLHRRCHWPQVHLPTPLDRRPRRRL 264
Query: 433 LPVQQQQQNQQARPTFPPIPMLYAELLPTLLHRGHCTTR 471
L ++ Q RP PP P L+ LLP L R H R
Sbjct: 265 LSLRHGHPKGQRRP--PPFPQLH--LLPRRLPRRHVPRR 369
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 31.6 bits (70), Expect = 1.5
Identities = 27/100 (27%), Positives = 42/100 (42%), Gaps = 11/100 (11%)
Frame = +3
Query: 390 PPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVPVYAIAQQMKQQL-------PVQQQQQNQ 442
PP P ++ P+F P H PP P +P +A+ + L P +++
Sbjct: 270 PPPPPPHHHNSLPYFNPTSHGLCEPPPPPPLPPP*LARSPQHALRRRPPPPPPPKRRS*T 449
Query: 443 QARPTFPPIPMLYAELLPTLLHRGHC----TTRQGKPAPD 478
+ P + + + E LP L H G C TR G+ PD
Sbjct: 450 RPPPPYSRLRLPPRESLPCLRH-GSCRPGTRTRLGRTRPD 566
>TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL protein
{Mus musculus;} , partial (3%)
Length = 1044
Score = 31.2 bits (69), Expect = 1.9
Identities = 25/76 (32%), Positives = 30/76 (38%), Gaps = 6/76 (7%)
Frame = +1
Query: 414 PPGQPQ-----VPVYAIAQQMKQQLPVQQQQQNQQARPT-FPPIPMLYAELLPTLLHRGH 467
PP PQ VP A A Q P +QQ+ Q P PP P L R
Sbjct: 31 PPPPPQAASVSVPTVAPAPSSSQPEPSPRQQREQHPIPVALPP---------PPPLSRAP 183
Query: 468 CTTRQGKPAPDPLPPR 483
R+ P+P PLP +
Sbjct: 184 PVARERLPSPPPLPEK 231
>BP029871
Length = 519
Score = 31.2 bits (69), Expect = 1.9
Identities = 26/74 (35%), Positives = 32/74 (43%), Gaps = 12/74 (16%)
Frame = +3
Query: 379 ATVAPINAAQMPPSYPY-VSYSQHPFFPPFYHQYPLPPG-----------QPQVPVYAIA 426
A+ AP N P PY S+ Q PF P H +PL QP + I
Sbjct: 174 ASSAPNNNTTFPSFDPYNPSHHQXPFQPNLLH-HPLHHKDEEKEENNNTVQPNPMNFQIE 350
Query: 427 QQMKQQLPVQQQQQ 440
+ +QQLP QQQQ
Sbjct: 351 RDQRQQLPQLQQQQ 392
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 31.2 bits (69), Expect = 1.9
Identities = 28/107 (26%), Positives = 38/107 (35%), Gaps = 4/107 (3%)
Frame = +2
Query: 383 PINAAQMPPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVPVYAIAQQMKQQLPVQQQQQNQ 442
P PP + ++ HP P + + P P P + Q Q Q +
Sbjct: 101 PTTTQSTPPP*SFHRHNLHPRHPLLHSRLGFHPRHPPPPHHHHHQATAQTTTTSQLSRLH 280
Query: 443 QARPTFPPIPMLYAELLPTLLHRGHCTTRQG--KPA--PDPLPPRFR 485
R L LP LLHR ++ +P P PLPPR R
Sbjct: 281 PHR--------LQRHSLPGLLHRRRLLLQRPPHRPTIHPHPLPPRLR 397
>TC18087 similar to UP|Q9LF46 (Q9LF46) 2-hydroxyphytanoyl-CoA lyase-like
protein, partial (30%)
Length = 571
Score = 30.8 bits (68), Expect = 2.5
Identities = 18/68 (26%), Positives = 34/68 (49%), Gaps = 3/68 (4%)
Frame = +2
Query: 27 AAQEQSATAIPIATAIPVTTSVLPTASTDARFAMPAGFPYGL---PPFFTPSTAAGTSGT 83
+A S T+ P+AT P+T ++ P ++ + F +PA L P+ TP + +
Sbjct: 167 SASSPSTTSSPLATPPPLTATLPPAPASTSLFPVPAASTASLASPTPWLTPGLLS*SPAP 346
Query: 84 ANNVLIPA 91
A +++ A
Sbjct: 347 AIRLMLAA 370
>AU251550
Length = 329
Score = 30.8 bits (68), Expect = 2.5
Identities = 14/32 (43%), Positives = 16/32 (49%)
Frame = +2
Query: 390 PPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVP 421
PP P Y+ P PPF H +P PP P P
Sbjct: 140 PPGPPRDPYAPAPPPPPFGHHHPPPPHGPPSP 235
>AV409168
Length = 243
Score = 26.6 bits (57), Expect(2) = 4.0
Identities = 16/44 (36%), Positives = 22/44 (49%), Gaps = 3/44 (6%)
Frame = +3
Query: 406 PFYHQYPLPPGQ---PQVPVYAIAQQMKQQLPVQQQQQNQQARP 446
P H + +P Q P P I+ Q+KQQ QQ QQ ++ P
Sbjct: 18 PVNHGFQIPKNQQQPPPPPPSPISSQLKQQ---QQHQQQWRSNP 140
Score = 21.9 bits (45), Expect(2) = 4.0
Identities = 8/18 (44%), Positives = 10/18 (55%)
Frame = +2
Query: 465 RGHCTTRQGKPAPDPLPP 482
R H + P+PDP PP
Sbjct: 146 RRHPRRTRQNPSPDPTPP 199
>TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.35
{Arabidopsis thaliana;} , partial (16%)
Length = 1208
Score = 30.0 bits (66), Expect = 4.3
Identities = 24/90 (26%), Positives = 40/90 (43%), Gaps = 8/90 (8%)
Frame = +3
Query: 36 IPIATAIPVTTSVLPTASTDARFAMPAGFPYGLPPFFTPSTAAGTSGTANNVLIPATNVV 95
IP + P + S PT++T A P PP TA TS +A +++ P ++
Sbjct: 306 IPPNPSSPPSASSSPTSTTSMIPPASANSPSSAPP--AAPTARSTSSSA-SLVAPNPSIT 476
Query: 96 SINATLPQTT--------AAVTEPLVHTMP 117
A P T +++TE ++H +P
Sbjct: 477 PTQAPTPTLTLTLTVTLRSSITEDILHHLP 566
>AV769784
Length = 535
Score = 30.0 bits (66), Expect = 4.3
Identities = 18/62 (29%), Positives = 22/62 (35%)
Frame = +3
Query: 389 MPPSYPYVSYSQHPFFPPFYHQYPLPPGQPQVPVYAIAQQMKQQLPVQQQQQNQQARPTF 448
+ PS S P F F+H P P Q Q +P+ QQ A PT
Sbjct: 252 LQPSISESHVSNSPLFVSFHHLPPSPSTSSQHSPPPYPPQSHSSVPIPSQQDQPSALPTP 431
Query: 449 PP 450
P
Sbjct: 432 KP 437
>AV409036
Length = 429
Score = 29.6 bits (65), Expect = 5.6
Identities = 14/42 (33%), Positives = 21/42 (49%)
Frame = +2
Query: 70 PFFTPSTAAGTSGTANNVLIPATNVVSINATLPQTTAAVTEP 111
P TPST + + T L PAT+ + T P+ ++ T P
Sbjct: 185 PAVTPSTPSSATTTTTTSLSPATSAKTAAVTGPKAASSATSP 310
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.345 0.151 0.521
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,376,961
Number of Sequences: 28460
Number of extensions: 653016
Number of successful extensions: 6850
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 6438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6808
length of query: 2334
length of database: 4,897,600
effective HSP length: 105
effective length of query: 2229
effective length of database: 1,909,300
effective search space: 4255829700
effective search space used: 4255829700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC147714.8