BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147714.15 + phase: 0
(663 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767093 194 3e-50
CB828089 181 1e-47
BI420290 117 6e-27
AV416701 117 8e-27
CB829290 110 7e-25
AU089197 70 3e-20
AV776921 70 1e-12
BP033858 68 5e-12
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 67 1e-11
BP055390 59 3e-09
TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 53 2e-07
BG662257 52 4e-07
AV411197 47 1e-05
AV418106 43 1e-04
AV418183 37 0.010
TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 36 0.017
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 35 0.039
AW720269 32 0.25
BI417718 32 0.43
BP055095 31 0.56
>AV767093
Length = 603
Score = 194 bits (494), Expect = 3e-50
Identities = 94/201 (46%), Positives = 134/201 (65%)
Frame = +1
Query: 282 RTFEEEDRFFQKNLDLIDINASSFFHNFASNEWLIQRLETISAGVLASAALCMVILPPGT 341
R F ++ F Q+N+D ++ + FHN +NEWL RL+ L + + M+ LP
Sbjct: 1 RGFRKQGEFCQENIDRVNASLRMDFHNNGANEWLGYRLDFTGVVFLCISTMFMIFLPSSI 180
Query: 342 FTSGFIGMALSYGLALNSFLVNSIQSQCTLANQIISVERLNQYMHIQSEAKEIVEGNRPP 401
++G++LSYGLAL+S L +I C + N+++SVER+ Q+ ++ SEA + PP
Sbjct: 181 VRPEYVGLSLSYGLALSSVLSFTISMTCNVENKMVSVERIKQFTNLPSEAPWKIPDLSPP 360
Query: 402 LNWPIAGKVEINDLKIRYRPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRLV 461
NWP G +E+N L++RYRP+ PLVL GI+ T + G KIG+VGRTGSGKSTLI LFRL+
Sbjct: 361 QNWPNHGSIELNSLQVRYRPNTPLVLKGISLTVQGGEKIGVVGRTGSGKSTLIQVLFRLI 540
Query: 462 EPSGGNIIIDGVDISSIGLHD 482
EPS G IIIDG++I ++GLHD
Sbjct: 541 EPSAGKIIIDGINICTLGLHD 603
>CB828089
Length = 511
Score = 181 bits (458), Expect(2) = 1e-47
Identities = 89/103 (86%), Positives = 97/103 (93%)
Frame = +1
Query: 520 LGKCQLREVVQEKDEGLNSSVVEDGSNWSMGQRQLFCLGRALLRRSRILVLDEATASVDN 579
LGKCQL+E VQEK+ GL+SSVVEDG+NWSMGQRQLFCLGRALLRRSRILVLDEATAS+DN
Sbjct: 1 LGKCQLQEAVQEKEGGLDSSVVEDGANWSMGQRQLFCLGRALLRRSRILVLDEATASIDN 180
Query: 580 STDYILLKTIRTEFADCTVITVAHRIPTVMDCTMVLSINDGNL 622
+TD IL KTIRTEFADCTVITVAHRIPTVMDCT VL+I+DG L
Sbjct: 181 ATDLILQKTIRTEFADCTVITVAHRIPTVMDCTKVLAISDGKL 309
Score = 26.6 bits (57), Expect(2) = 1e-47
Identities = 11/15 (73%), Positives = 15/15 (99%)
Frame = +2
Query: 620 GNLSRNTGLIFSLQS 634
G+LS++TGLIFSLQ+
Sbjct: 362 GSLSKSTGLIFSLQN 406
>BI420290
Length = 398
Score = 117 bits (293), Expect = 6e-27
Identities = 61/129 (47%), Positives = 80/129 (61%)
Frame = +2
Query: 326 VLASAALCMVILPPGTFTSGFIGMALSYGLALNSFLVNSIQSQCTLANQIISVERLNQYM 385
V A + + +V LP G G+A++YG+ LN + I + C N++ISVER+ QY
Sbjct: 11 VFAFSLVMLVSLPEGIINPSIAGLAVTYGINLNVLQASVIWNICNAENKMISVERILQYT 190
Query: 386 HIQSEAKEIVEGNRPPLNWPIAGKVEINDLKIRYRPDGPLVLHGITCTFEVGHKIGIVGR 445
HI SEA ++E +PP NWP G + +L+IRY P VL ITCTF KIG+VGR
Sbjct: 191 HIASEAPLVIEDCKPPSNWPETGTICFKNLQIRYAEHLPSVLKNITCTFPGRKKIGVVGR 370
Query: 446 TGSGKSTLI 454
TGSGKSTLI
Sbjct: 371 TGSGKSTLI 397
>AV416701
Length = 406
Score = 117 bits (292), Expect = 8e-27
Identities = 58/133 (43%), Positives = 81/133 (60%)
Frame = +2
Query: 302 ASSFFHNFASNEWLIQRLETISAGVLASAALCMVILPPGTFTSGFIGMALSYGLALNSFL 361
A FF + A+ EWL R+E +S V + + +V P G G+A +YGL LNS L
Sbjct: 8 ARPFFCSIAAIEWLCLRMELLSTFVFSFCMVLLVSFPRGNIDPSMAGLAATYGLNLNSRL 187
Query: 362 VNSIQSQCTLANQIISVERLNQYMHIQSEAKEIVEGNRPPLNWPIAGKVEINDLKIRYRP 421
I S C L N+IIS+ER+ QY + SEA ++E RP WP G +++ DLK+RY+
Sbjct: 188 SRRILSFCKLENKIISIERIYQYSQVPSEAPSVIEDFRPTSTWPENGTIQLIDLKVRYKE 367
Query: 422 DGPLVLHGITCTF 434
+ PLVLHG++CTF
Sbjct: 368 NLPLVLHGVSCTF 406
>CB829290
Length = 546
Score = 110 bits (275), Expect = 7e-25
Identities = 52/89 (58%), Positives = 72/89 (80%)
Frame = -3
Query: 532 KDEGLNSSVVEDGSNWSMGQRQLFCLGRALLRRSRILVLDEATASVDNSTDYILLKTIRT 591
K+ L+S+V E+G NWSMGQRQL CLGR LL++S++LVLDEATASVD +TD ++ +T+R
Sbjct: 544 KEGKLDSTVSENGENWSMGQRQLVCLGRVLLKKSKVLVLDEATASVDTATDNLIQQTLRQ 365
Query: 592 EFADCTVITVAHRIPTVMDCTMVLSINDG 620
F++ TVIT+AHRI +V+D MVL ++ G
Sbjct: 364 HFSESTVITIAHRITSVIDSDMVLLLSQG 278
>AU089197
Length = 658
Score = 70.5 bits (171), Expect(2) = 3e-20
Identities = 36/71 (50%), Positives = 47/71 (65%)
Frame = +1
Query: 405 PIAGKVEINDLKIRYRPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRLVEPS 464
P G +E++ L+++Y P P+VL +T F KIGI RTGSGKSTL+ ALF +V P
Sbjct: 343 PRDGMIELHKLQVQYDPAAPIVLKDVTFIFSGQKKIGIXXRTGSGKSTLLQALF*IVXPL 522
Query: 465 GGNIIIDGVDI 475
G I+IDGV I
Sbjct: 523 SGCILIDGVXI 555
Score = 45.1 bits (105), Expect(2) = 3e-20
Identities = 21/30 (70%), Positives = 24/30 (80%)
Frame = +3
Query: 476 SSIGLHDLRSRFGIIPQDPTLFTGTVRYNL 505
S IGL DLR++ GIIPQDPTLF TVR +L
Sbjct: 555 SKIGLQDLRTKIGIIPQDPTLFLXTVRTDL 644
>AV776921
Length = 435
Score = 69.7 bits (169), Expect = 1e-12
Identities = 44/135 (32%), Positives = 71/135 (52%), Gaps = 1/135 (0%)
Frame = +2
Query: 420 RPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRLVEPSGGNIIIDGVDISSIG 479
RPD +L+ + G + +VG +GSGKST+IS + R EP G+I++DG DI +
Sbjct: 26 RPDVE-ILNKLCLDIPSGKIVALVGGSGSGKSTVISLIERFYEPLSGDILLDGNDIRDLD 202
Query: 480 LHDLRSRFGIIPQDPTLFTGTVRYN-LDPLSQYSDQEIWEVLGKCQLREVVQEKDEGLNS 538
L LR + G++ Q+P LF +++ N L + +E+ + + + E L +
Sbjct: 203 LKWLRQQIGLVNQEPALFATSIKENILYGKDNATLEELKRAVKLSDAQSFINNLPERLET 382
Query: 539 SVVEDGSNWSMGQRQ 553
V E G S GQ+Q
Sbjct: 383 QVGERGIQLSGGQKQ 427
>BP033858
Length = 533
Score = 67.8 bits (164), Expect = 5e-12
Identities = 31/55 (56%), Positives = 43/55 (77%)
Frame = -1
Query: 566 RILVLDEATASVDNSTDYILLKTIRTEFADCTVITVAHRIPTVMDCTMVLSINDG 620
R+L +DEATASVD+ TD ++ + IR +FA T+I++AHRIPTVMDC VL ++ G
Sbjct: 533 RLLFMDEATASVDSQTDAVIQRIIREDFAARTIISIAHRIPTVMDCDRVLVVDAG 369
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 66.6 bits (161), Expect = 1e-11
Identities = 35/88 (39%), Positives = 52/88 (58%)
Frame = +3
Query: 418 RYRPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRLVEPSGGNIIIDGVDISS 477
++ DG +L GI G +G++G +GSGKSTL+ AL RL EP ++ +D DI
Sbjct: 111 KFSDDGVPILKGIHLDIPKGVIVGVIGPSGSGKSTLLRALNRLWEPPSASVFLDARDICH 290
Query: 478 IGLHDLRSRFGIIPQDPTLFTGTVRYNL 505
+ + LR + G++ Q P LF GTV N+
Sbjct: 291 LDVLSLRRKVGMLFQLPALFEGTVADNV 374
>BP055390
Length = 488
Score = 58.5 bits (140), Expect = 3e-09
Identities = 32/107 (29%), Positives = 60/107 (55%), Gaps = 6/107 (5%)
Frame = +3
Query: 522 KCQLREVVQEKDEGLN------SSVVEDGSNWSMGQRQLFCLGRALLRRSRILVLDEATA 575
KC L +V + +N + V + G S GQ+Q + RA+L+ IL+LDEAT+
Sbjct: 138 KCALCILVMLTKDSVN*CSNLLTQVGQRGVQLSGGQKQRIAIARAILKNPPILLLDEATS 317
Query: 576 SVDNSTDYILLKTIRTEFADCTVITVAHRIPTVMDCTMVLSINDGNL 622
++D ++ ++ + + T TVI +AHR+ TV++ ++ + +G +
Sbjct: 318 ALDTESEKLVQEALETAMHGRTVILIAHRLSTVVNADVIAVVENGQV 458
>TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(9%)
Length = 712
Score = 52.8 bits (125), Expect = 2e-07
Identities = 25/86 (29%), Positives = 51/86 (59%)
Frame = +2
Query: 535 GLNSSVVEDGSNWSMGQRQLFCLGRALLRRSRILVLDEATASVDNSTDYILLKTIRTEFA 594
G ++ V E G+ S GQ+Q + RA+++ IL+LDEAT+++D ++ ++ +
Sbjct: 71 GYDTVVGERGTLLSGGQKQRVAIARAIIKTPNILLLDEATSALDAESERVVQDALDKVMV 250
Query: 595 DCTVITVAHRIPTVMDCTMVLSINDG 620
+ T + VAHR+ T+ + ++ + +G
Sbjct: 251 NRTTVIVAHRLSTIKNADVITVLKNG 328
>BG662257
Length = 336
Score = 51.6 bits (122), Expect = 4e-07
Identities = 27/94 (28%), Positives = 53/94 (55%), Gaps = 3/94 (3%)
Frame = +1
Query: 518 EVLGKCQL---REVVQEKDEGLNSSVVEDGSNWSMGQRQLFCLGRALLRRSRILVLDEAT 574
E++ +L + + +G ++ V + G S GQ+Q + RA+++ +IL+LDEAT
Sbjct: 43 EIIAAAELANAHKFISSLQQGYDTVVGDRGIQLSGGQKQRVAIARAIVKSPKILLLDEAT 222
Query: 575 ASVDNSTDYILLKTIRTEFADCTVITVAHRIPTV 608
+++D ++ ++ + D T I VAHR+ T+
Sbjct: 223 SALDAESEKVVQDALDRVRVDRTTIVVAHRLSTI 324
>AV411197
Length = 391
Score = 47.0 bits (110), Expect = 1e-05
Identities = 26/104 (25%), Positives = 55/104 (52%), Gaps = 1/104 (0%)
Frame = +3
Query: 489 IIPQDPTLFTGTVRYNLD-PLSQYSDQEIWEVLGKCQLREVVQEKDEGLNSSVVEDGSNW 547
+I Q+P +F+ T+R N+ S+ E+ E + G ++ V G +
Sbjct: 54 LIQQEPIIFSTTIRENIIYARHNASEAEMKEAARIANAHHFISSLPHGYDTHVGMRGVDL 233
Query: 548 SMGQRQLFCLGRALLRRSRILVLDEATASVDNSTDYILLKTIRT 591
+ GQ+Q + R +L+ + IL+LDEA++S+++ + ++ + + T
Sbjct: 234 TPGQKQRIAIARVVLKNAPILLLDEASSSIESESSRVIQEALDT 365
>AV418106
Length = 419
Score = 43.1 bits (100), Expect = 1e-04
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -2
Query: 592 EFADCTVITVAHRIPTVMDCTMVLSINDG 620
+FAD T++++AHRIPTVMDC VL I+ G
Sbjct: 415 DFADRTIVSIAHRIPTVMDCDRVLVIDAG 329
>AV418183
Length = 298
Score = 37.0 bits (84), Expect = 0.010
Identities = 27/100 (27%), Positives = 43/100 (43%), Gaps = 4/100 (4%)
Frame = +3
Query: 472 GVDISSIGLHDLRSRFGIIPQDPTLFTGTVR----YNLDPLSQYSDQEIWEVLGKCQLRE 527
GV ++ I L + I+ Q+PTLF ++ Y D + + +I E
Sbjct: 3 GVPLAEISHRHLHRKISIVSQEPTLFNCSIEENIAYGFD--GKLNSVDIENAAKMANAHE 176
Query: 528 VVQEKDEGLNSSVVEDGSNWSMGQRQLFCLGRALLRRSRI 567
+ + E + V E G S GQ+Q + RALL +I
Sbjct: 177 FISKFPEKYQTFVGERGVRLSGGQKQRIAIARALLMDPKI 296
>TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(7%)
Length = 552
Score = 36.2 bits (82), Expect = 0.017
Identities = 14/59 (23%), Positives = 34/59 (56%)
Frame = +3
Query: 562 LRRSRILVLDEATASVDNSTDYILLKTIRTEFADCTVITVAHRIPTVMDCTMVLSINDG 620
++ IL+LDE T+++D ++ ++ + + T + VAHR+ T+ +++ + +G
Sbjct: 3 IKSPNILLLDEXTSALDVESERVVQDALDKVMVNRTTVIVAHRLSTIKSADVIIVLKNG 179
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 35.0 bits (79), Expect = 0.039
Identities = 29/98 (29%), Positives = 43/98 (43%), Gaps = 4/98 (4%)
Frame = +1
Query: 410 VEINDLKIRYRPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRL--VEPSGGN 467
+++NDLK + + +L G+ T G ++G GSGKST L E +GG
Sbjct: 163 LQVNDLKAKIVGEHKDLLKGLNLTINRGEVHALMGANGSGKSTFAKVLVGHPDYEVTGGR 342
Query: 468 IIIDGVDISSIGLHDLRSRFGIIP--QDPTLFTGTVRY 503
+ G ++ + D RS GI Q P G Y
Sbjct: 343 AVYKGENLLDMEPVD-RSLAGIFMSLQSPVEIPGVSNY 453
>AW720269
Length = 524
Score = 32.3 bits (72), Expect = 0.25
Identities = 39/170 (22%), Positives = 76/170 (43%), Gaps = 12/170 (7%)
Frame = +3
Query: 420 RPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTLISALFRLVEPSGGNIIIDGVDISSIG 479
+P +L ++ + +VG +G+GKSTL+ + G + D +I
Sbjct: 36 KPKPVNILKSVSFVARSSEIVAVVGPSGTGKSTLLRII-------AGRVKDRDFDPKTIS 194
Query: 480 LHD--------LRSRFGIIPQDPTLF-TGTVRYNLDPLSQYSDQEIWEVLGKCQLREVVQ 530
++D LR G + Q+ L TV+ L +++ +E+ + ++ ++Q
Sbjct: 195 INDHPMTSPAQLRKICGFVAQEDNLLPLLTVKETLLFSAKFRLKEMTPNDREMRVESLMQ 374
Query: 531 EKD-EGLNSSVVEDGSN--WSMGQRQLFCLGRALLRRSRILVLDEATASV 577
E ++ S V D N S G+R+ +G ++ IL+LDE T+ +
Sbjct: 375 ELGLFHVSDSFVGDEENRGISGGERKRVSIGVDMIHNPPILLLDEPTSGL 524
>BI417718
Length = 515
Score = 31.6 bits (70), Expect = 0.43
Identities = 17/48 (35%), Positives = 27/48 (55%), Gaps = 2/48 (4%)
Frame = +2
Query: 408 GKVEINDLKIRY--RPDGPLVLHGITCTFEVGHKIGIVGRTGSGKSTL 453
GK+E + +Y RPD + ++ T G + +VG +GSGKST+
Sbjct: 374 GKIEFCHVSFKYPSRPDIQ-IFPDLSLTIHAGTTVALVGESGSGKSTV 514
>BP055095
Length = 538
Score = 31.2 bits (69), Expect = 0.56
Identities = 19/70 (27%), Positives = 36/70 (51%)
Frame = +3
Query: 548 SMGQRQLFCLGRALLRRSRILVLDEATASVDNSTDYILLKTIRTEFADCTVITVAHRIPT 607
S GQ++ G L+ ++ L +DE + +D+ST + + K +R ++
Sbjct: 105 SGGQKKRVTTGEMLVGPAKALFMDEISTGLDSSTTFQICKFMR-------------QMVH 245
Query: 608 VMDCTMVLSI 617
+MD TMV+S+
Sbjct: 246 IMDVTMVISL 275
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,352,279
Number of Sequences: 28460
Number of extensions: 135242
Number of successful extensions: 721
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 718
length of query: 663
length of database: 4,897,600
effective HSP length: 96
effective length of query: 567
effective length of database: 2,165,440
effective search space: 1227804480
effective search space used: 1227804480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147714.15