BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147714.1 - phase: 0 /pseudo
(805 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE122516 92 2e-19
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 87 1e-17
AU251673 57 1e-08
AV410603 53 2e-07
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 53 2e-07
BI418821 52 5e-07
BG662087 49 4e-06
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 41 9e-04
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 40 0.001
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 40 0.002
AV779679 34 0.10
TC20066 similar to GB|AAM91455.1|22137220|AY133625 AT3g11400/F24... 32 0.52
BP039068 32 0.52
TC17929 32 0.52
BP043432 30 1.5
TC9283 29 3.4
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 28 4.4
AV773507 28 7.5
TC16357 weakly similar to UP|Q8DZP0 (Q8DZP0) Bacterial luciferas... 28 7.5
TC9401 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans is... 28 7.5
>BE122516
Length = 364
Score = 92.4 bits (228), Expect = 2e-19
Identities = 48/115 (41%), Positives = 73/115 (62%)
Frame = +2
Query: 601 LGMNWLEYNHVLINCFSKSVHFSSVEEESGAEFLSTKQLKQLERDGILMFSLMATLSIEN 660
+GMNWL N +NC K+V F + E ++ + K K E + ++ + T ++
Sbjct: 14 VGMNWLTANDATLNCRKKTVTFGTSEGDAKRVKRTDKVGKASECESDVLLGALET--DKS 187
Query: 661 QAVIDRLPVVCDFPEVFPDEIPDVPPEREVEFSIDLVPGTKPVSMAPYRISASEL 715
++ +PVV +F +VFP+E+ ++PPEREVEFSID VPGT P+S+APYR+S EL
Sbjct: 188 DTGVEGIPVVREFSDVFPEEVSELPPEREVEFSID*VPGTGPISIAPYRMSLVEL 352
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 86.7 bits (213), Expect = 1e-17
Identities = 48/155 (30%), Positives = 72/155 (45%), Gaps = 7/155 (4%)
Frame = -2
Query: 395 RKGKGQQSRPKPYSAPADKGKQKMVD------VRRPKKKDAAEIVCFNCGEKGHKSNACP 448
R + + + KP+ P ++G V RP + D +EIVC C +KGH +N CP
Sbjct: 464 RFDRNKSFQKKPFQRPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCP 285
Query: 449 EEIKKCVRCGKKGHVVADCNRTDI-VCFNCNGEGHISSQCTQPKRAPTTGRVFALTGTQT 507
+ + C C K GH DC + N + + KR + RV+ ++G ++
Sbjct: 284 DLV--CWNCQKTGHSGKDCTNPKVEAATNAIAARRPAPAANKGKRPVASARVYTVSGAES 111
Query: 508 ENEDRLIRGTCYINNTPLVAIIDTGATHCFIAFDC 542
D LIR +N PL + D+GATH FI C
Sbjct: 110 HRADGLIRSVGSVNCKPLTILFDSGATHSFIDLAC 6
>AU251673
Length = 413
Score = 57.0 bits (136), Expect = 1e-08
Identities = 32/102 (31%), Positives = 50/102 (48%), Gaps = 1/102 (0%)
Frame = +2
Query: 35 CTEEQKVRFGTHQLAEAADDWWVALLPTLGQEGAVVTWAVFRREFLRRYFPEDVRGKKEI 94
C++ + V + QL A DW+ L TWA F EF+ R+ P+ VR
Sbjct: 5 CSDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVR 184
Query: 95 EFLELKQG-NMSVTEYAAQFVELSKFYPHYTGENAEFSRCIK 135
+F L+Q M+V+EY+A F LS++ P+ E R ++
Sbjct: 185 DFERLEQAEGMTVSEYSAHFTHLSRYVPYPLLEEERVKRFVR 310
Score = 50.8 bits (120), Expect = 8e-07
Identities = 35/137 (25%), Positives = 64/137 (46%), Gaps = 1/137 (0%)
Frame = +2
Query: 251 TEEQKVRFGTHQLAEEADDWWVALLPTLGQEGAVMTWAVFRREFLRRYFLEDVRGKKEIE 310
++ + V + QL A DW+ L TWA F EF+ R+ + VR +
Sbjct: 8 SDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRD 187
Query: 311 FLELKQG-NMSVTEYAAKFVELSKFYPHYTAENAEFSRCIKFENGLRLDIKRAIGYQQLR 369
F L+Q M+V+EY+A F LS++ P+ E R +F GL+ + +++ +
Sbjct: 188 FERLEQAEGMTVSEYSAHFTHLSRYVPYPLLEE---ERVKRFVRGLKEYLFKSVVGSKSS 358
Query: 370 VFQDLVNSCRIYEEDTK 386
++++ + E+ K
Sbjct: 359 TLSEVLSLALLVEQRQK 409
>AV410603
Length = 162
Score = 53.1 bits (126), Expect = 2e-07
Identities = 20/35 (57%), Positives = 31/35 (88%)
Frame = +1
Query: 762 TIKNRYPLPRIDDLMDQLVGARVFSKIDLRSGYHQ 796
T+K+ +P+P +D+L+D+L G++ FSK+DLRSGYHQ
Sbjct: 4 TVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQ 108
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 53.1 bits (126), Expect = 2e-07
Identities = 25/93 (26%), Positives = 45/93 (47%), Gaps = 1/93 (1%)
Frame = +2
Query: 396 KGKGQQSRPKPYSAPADKGKQKMVDVRRPKKKD-AAEIVCFNCGEKGHKSNACPEEIKKC 454
+ + + P +D+ + RR ++ + + +C NC GH + CP + C
Sbjct: 227 RSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPN-VAIC 403
Query: 455 VRCGKKGHVVADCNRTDIVCFNCNGEGHISSQC 487
CG GH+ ++C T +C+NC GH++S C
Sbjct: 404 HNCGLPGHIASECT-TKSLCWNCKEPGHMASSC 499
Score = 36.6 bits (83), Expect = 0.016
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +2
Query: 454 CVRCGKKGHVVADCNRTDIVCFNCNGEGHISSQCT 488
C C + GH +C I C NC GHI+S+CT
Sbjct: 344 CKNCKRPGHFARECPNVAI-CHNCGLPGHIASECT 445
Score = 28.5 bits (62), Expect = 4.4
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +2
Query: 433 VCFNCGEKGHKSNACPEE 450
+C+NC E GH +++CP +
Sbjct: 455 LCWNCKEPGHMASSCPND 508
>BI418821
Length = 614
Score = 51.6 bits (122), Expect = 5e-07
Identities = 22/71 (30%), Positives = 32/71 (44%), Gaps = 15/71 (21%)
Frame = +2
Query: 434 CFNCGEKGHKSNACPEEIKK--------CVRCGKKGHVVADCNRTD-------IVCFNCN 478
C+NCG+ GH + C C CG GH+ DCNR++ C+NC
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCG 571
Query: 479 GEGHISSQCTQ 489
GH++ C +
Sbjct: 572 DTGHLARDCNR 604
Score = 40.8 bits (94), Expect = 9e-04
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 7/45 (15%)
Frame = +2
Query: 434 CFNCGEKGHKSNACPEEIKK-------CVRCGKKGHVVADCNRTD 471
C+NCG+ GH + C C CG GH+ DCNR++
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>BG662087
Length = 373
Score = 48.5 bits (114), Expect = 4e-06
Identities = 23/50 (46%), Positives = 29/50 (58%)
Frame = +1
Query: 747 GSMRLCIDYRQLNKVTIKNRYPLPRIDDLMDQLVGARVFSKIDLRSGYHQ 796
G R+ +DY LNK K+ YPLP ID L+D + S +D SGYHQ
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQ 165
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 40.8 bits (94), Expect = 9e-04
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +1
Query: 434 CFNCGEKGHKSNACP--EEIKKCVRCGKKGHVVADCNRT 470
CFNCG GH + C + KC RCG++GH+ +C +
Sbjct: 391 CFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNS 507
Score = 35.0 bits (79), Expect = 0.047
Identities = 16/43 (37%), Positives = 19/43 (43%), Gaps = 3/43 (6%)
Frame = +1
Query: 453 KCVRCGKKGHVVADCNRTD--IVCFNCNGEGHISSQC-TQPKR 492
+C CG GH DC D C+ C GHI C PK+
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKK 516
Score = 27.7 bits (60), Expect = 7.5
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +1
Query: 434 CFNCGEKGHKSNACPEEIKKCVR 456
C+ CGE+GH C KK R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 40.4 bits (93), Expect = 0.001
Identities = 17/39 (43%), Positives = 22/39 (55%), Gaps = 2/39 (5%)
Frame = +2
Query: 434 CFNCGEKGHKSNACP--EEIKKCVRCGKKGHVVADCNRT 470
CFNCG GH + C + KC RCG +GHV +C +
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNS 487
Score = 37.7 bits (86), Expect = 0.007
Identities = 21/72 (29%), Positives = 29/72 (40%), Gaps = 5/72 (6%)
Frame = +2
Query: 438 GEKGHKSNACPEEIKKCVRCGKKGHVVADCNRTD--IVCFNCNGEGHISSQC-TQPKRA- 493
G + + P +C CG GH DC D C+ C GH+ C PK+
Sbjct: 323 GNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNE 502
Query: 494 -PTTGRVFALTG 504
T +F+ TG
Sbjct: 503 WQTWKELFSFTG 538
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 39.7 bits (91), Expect = 0.002
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Frame = +3
Query: 454 CVRCGKKGHVVADCNRTDIVCFNCNGEGHISSQCT----QPKRAPTTGR 498
C C + GH+ DC ++C NC G GH++ +C + AP T R
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSGRFMDRYAPNTRR 155
Score = 38.5 bits (88), Expect = 0.004
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = +3
Query: 432 IVCFNCGEKGHKSNACPEEIKKCVRCGKKGHVVADC 467
+VC NC + GH S C + C CG +GH+ +C
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 27.3 bits (59), Expect = 9.8
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +3
Query: 432 IVCFNCGEKGHKSNACP 448
++C NCG +GH + CP
Sbjct: 63 MICHNCGGRGHLAYECP 113
>AV779679
Length = 440
Score = 33.9 bits (76), Expect = 0.10
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +3
Query: 748 SMRLCIDYRQLNKVTIKNRYPLPRIDDLMD 777
+M+LC DY QL+ VTI N+ LP +D+ D
Sbjct: 345 TMQLCDDYMQLDYVTIPNKSLLPHLDEWSD 434
>TC20066 similar to GB|AAM91455.1|22137220|AY133625 AT3g11400/F24K9_7
{Arabidopsis thaliana;}, partial (45%)
Length = 557
Score = 31.6 bits (70), Expect = 0.52
Identities = 18/47 (38%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = +2
Query: 403 RPKPYSAPADKGKQKMVDVRRPKKKDAAEIVCFNCGEKG-HKSNACP 448
RPKP A ++ K P +K A +VC CG+KG H ++ CP
Sbjct: 392 RPKPLGAKTEEPKAS----GDPFQKGAVLMVCRTCGKKGDHWTSRCP 520
>BP039068
Length = 467
Score = 31.6 bits (70), Expect = 0.52
Identities = 15/47 (31%), Positives = 21/47 (43%)
Frame = +3
Query: 431 EIVCFNCGEKGHKSNACPEEIKKCVRCGKKGHVVADCNRTDIVCFNC 477
+ VC NC E GH SN CP + R + + T + C +C
Sbjct: 303 QTVCMNCQETGHASNDCPSLTLRS-RNAQHPRMNQQSGETSVSCSSC 440
>TC17929
Length = 791
Score = 31.6 bits (70), Expect = 0.52
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +2
Query: 434 CFNCGEKGHKSNACPEE 450
C+ CGE GHK CP+E
Sbjct: 62 CYRCGESGHKMRNCPKE 112
>BP043432
Length = 487
Score = 30.0 bits (66), Expect = 1.5
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 4/43 (9%)
Frame = -3
Query: 438 GEKGHKSNACPEEI----KKCVRCGKKGHVVADCNRTDIVCFN 476
G K + N PE++ KK + CG V C+ ++VC N
Sbjct: 350 GHKKKRKNETPEDLERKKKKLIECGTVADAVRTCDICNVVCNN 222
>TC9283
Length = 1307
Score = 28.9 bits (63), Expect = 3.4
Identities = 12/42 (28%), Positives = 24/42 (56%)
Frame = +2
Query: 241 IERIFRVMQYTEEQKVRFGTHQLAEEADDWWVALLPTLGQEG 282
+ER+ R M E+ ++ +G H L+ ++ A++ L +EG
Sbjct: 905 VERLCRAMGGAEKVEIEYGNHSLSNRVEEAVQAIIDFLKREG 1030
Score = 28.5 bits (62), Expect = 4.4
Identities = 12/42 (28%), Positives = 24/42 (56%)
Frame = +2
Query: 26 IERIFRVMQCTEEQKVRFGTHQLAEAADDWWVALLPTLGQEG 67
+ER+ R M E+ ++ +G H L+ ++ A++ L +EG
Sbjct: 905 VERLCRAMGGAEKVEIEYGNHSLSNRVEEAVQAIIDFLKREG 1030
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 28.5 bits (62), Expect = 4.4
Identities = 9/17 (52%), Positives = 11/17 (63%)
Frame = +2
Query: 434 CFNCGEKGHKSNACPEE 450
CF CG+ GH + CP E
Sbjct: 419 CFKCGKPGHFARECPSE 469
>AV773507
Length = 496
Score = 27.7 bits (60), Expect = 7.5
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +2
Query: 434 CFNCGEKGHKSNACP 448
CF CG GH++ CP
Sbjct: 350 CFECGRPGHRARDCP 394
>TC16357 weakly similar to UP|Q8DZP0 (Q8DZP0) Bacterial luciferase family
protein, partial (6%)
Length = 572
Score = 27.7 bits (60), Expect = 7.5
Identities = 15/39 (38%), Positives = 20/39 (50%), Gaps = 2/39 (5%)
Frame = -2
Query: 536 CFIAFDCVSALGLDLSDMNGEMVV--ETPAKGSVTTSLV 572
CF+ FDC L D D NGE+ E +G V +L+
Sbjct: 217 CFLIFDCGVLLSKDW*DQNGELQKD*EADRRGQVQKNLL 101
>TC9401 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans isomerase ,
partial (97%)
Length = 918
Score = 27.7 bits (60), Expect = 7.5
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
Frame = +1
Query: 682 PDVPPEREVEFSIDLV-----PGTKPVSMAPYRISASELKKQLE 720
PD+PP+ + F ++L+ G+ S+ R ELKKQ E
Sbjct: 469 PDIPPDATLVFEVELLACNPRKGSSLGSVTEERARLEELKKQRE 600
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,951,454
Number of Sequences: 28460
Number of extensions: 169019
Number of successful extensions: 832
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 710
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of query: 805
length of database: 4,897,600
effective HSP length: 98
effective length of query: 707
effective length of database: 2,108,520
effective search space: 1490723640
effective search space used: 1490723640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC147714.1