BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147538.1 + phase: 0
(138 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW719998 119 1e-28
AV411385 29 0.33
TC8203 similar to UP|Q8L9C3 (Q8L9C3) Copia-like retroelement pol... 28 0.57
TC15586 weakly similar to UP|Q8LEJ2 (Q8LEJ2) Nematode resistance... 28 0.57
BP054628 25 4.8
TC13680 similar to UP|Q84V02 (Q84V02) Vacuolar membrane ATPase s... 25 6.3
TC9530 similar to UP|BAD10144 (BAD10144) CAAX amino terminal pro... 25 6.3
AV775170 25 6.3
AV407886 24 8.2
TC8217 similar to UP|Q8H6S5 (Q8H6S5) CTV.2, partial (8%) 24 8.2
BP084835 24 8.2
>AW719998
Length = 476
Score = 119 bits (299), Expect = 1e-28
Identities = 61/106 (57%), Positives = 77/106 (72%), Gaps = 10/106 (9%)
Frame = +2
Query: 1 MSKGGETVKQSEASSPLKQVDSAIEKT----------KGETADGDKDEIIKELKKVKKQN 50
MSK E +KQSEAS+P + S E+T +G + + DEI+KELKKVKKQN
Sbjct: 158 MSKSDEMLKQSEASTPSEPEASVPERTVSKNKDAGEVEGRSRGSEIDEIVKELKKVKKQN 337
Query: 51 FVTHCLLSAMIVLTVAWQLSEVSLVMRVKDGMNHPFRSVGNMFKEM 96
FVTHCLLS MIVLT AWQLS+VS++++V+DG+NHPFRS G+M K M
Sbjct: 338 FVTHCLLSVMIVLTAAWQLSQVSMMLKVRDGLNHPFRSFGSMIKRM 475
>AV411385
Length = 416
Score = 28.9 bits (63), Expect = 0.33
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = -1
Query: 40 IKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSLVMRVKDGMNHPFRSV 89
+K LK +K+NF + + + +LS + + R+++ MN+P R +
Sbjct: 215 MKRLKYEEKRNFSNTIIRLVAAFIQIKIKLSSIPEIRRIEESMNNPNRQM 66
>TC8203 similar to UP|Q8L9C3 (Q8L9C3) Copia-like retroelement pol
polyprotein, partial (96%)
Length = 1198
Score = 28.1 bits (61), Expect = 0.57
Identities = 12/37 (32%), Positives = 23/37 (61%)
Frame = +2
Query: 11 SEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVK 47
+E + +KQ+ A+ G AD D+DE+ EL++++
Sbjct: 611 NEQTENMKQIQEALSAPIGAAADFDEDELEAELEELE 721
>TC15586 weakly similar to UP|Q8LEJ2 (Q8LEJ2) Nematode resistance
protein-like protein, partial (32%)
Length = 773
Score = 28.1 bits (61), Expect = 0.57
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1
Query: 114 NNESTSLPSLKIPDMTNVDV 133
+N STSLPSL +P +TN ++
Sbjct: 142 SNNSTSLPSLNLPFLTNSEI 201
>BP054628
Length = 508
Score = 25.0 bits (53), Expect = 4.8
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -3
Query: 41 KELKKVKKQNFVTHCLLSAMIV 62
K +KKV Q F T C L AMIV
Sbjct: 281 KYIKKVYLQLFSTECSLCAMIV 216
>TC13680 similar to UP|Q84V02 (Q84V02) Vacuolar membrane ATPase subunit c'',
partial (18%)
Length = 536
Score = 24.6 bits (52), Expect = 6.3
Identities = 12/38 (31%), Positives = 18/38 (46%)
Frame = -2
Query: 49 QNFVTHCLLSAMIVLTVAWQLSEVSLVMRVKDGMNHPF 86
+ V C L+ ++ L AW L V L+ V G P+
Sbjct: 379 RGLVMICFLTGLVALACAWLLRLVRLLSFVFSGFPLPY 266
>TC9530 similar to UP|BAD10144 (BAD10144) CAAX amino terminal protease
family-like protein, partial (32%)
Length = 626
Score = 24.6 bits (52), Expect = 6.3
Identities = 11/35 (31%), Positives = 19/35 (53%)
Frame = +3
Query: 29 GETADGDKDEIIKELKKVKKQNFVTHCLLSAMIVL 63
GET + + D +++ L + N T CL+ + VL
Sbjct: 3 GETPERETDALVRLLPLIGSSNLSTACLVGIVGVL 107
>AV775170
Length = 502
Score = 24.6 bits (52), Expect = 6.3
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -2
Query: 9 KQSEASSPLKQVDSAIEKTKGETADGDK 36
KQ EAS+P DSA E++K + DK
Sbjct: 384 KQGEASNPPSDHDSAEERSKWGRREIDK 301
>AV407886
Length = 413
Score = 24.3 bits (51), Expect = 8.2
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -2
Query: 52 VTHCLLSAMIVLTVAWQLSEVSLV 75
+T+C L + T++W+L+ VS V
Sbjct: 304 ITYCELCFPFIKTLSWELTSVSKV 233
>TC8217 similar to UP|Q8H6S5 (Q8H6S5) CTV.2, partial (8%)
Length = 634
Score = 24.3 bits (51), Expect = 8.2
Identities = 14/44 (31%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Frame = +3
Query: 55 CLLSAMIVLTVAWQLSEVSLVM-RVKDGMNHPFRSVGNMFKEMV 97
CLL +V WQL +V L+ ++KD + P + + F +V
Sbjct: 228 CLLRLRMVQQAMWQLLQVDLLRNKLKDDDDDPCQLEASFFYILV 359
>BP084835
Length = 450
Score = 24.3 bits (51), Expect = 8.2
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = -3
Query: 69 LSEVSLVMRVKDGMNHPFRSVGNMFKEMVKDVSGSNGQDAAGDK 112
LS+V L +K ++ +S+G M+K++ K+++ + DK
Sbjct: 427 LSKVDLRKMLKSSLSGVDKSIGAMYKKLQKNLTSEELLPSLWDK 296
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.308 0.127 0.343
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,774,206
Number of Sequences: 28460
Number of extensions: 17355
Number of successful extensions: 88
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of query: 138
length of database: 4,897,600
effective HSP length: 81
effective length of query: 57
effective length of database: 2,592,340
effective search space: 147763380
effective search space used: 147763380
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 50 (23.9 bits)
Medicago: description of AC147538.1