BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147537.12 + phase: 0
(125 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11986 weakly similar to UP|Q8RYA8 (Q8RYA8) Lipid transfer prec... 111 4e-26
TC14628 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-tran... 97 8e-22
TC8034 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-trans... 96 2e-21
TC8033 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-trans... 94 5e-21
TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lip... 94 9e-21
TC19050 weakly similar to UP|Q9M6T9 (Q9M6T9) Nonspecific lipid-t... 90 1e-19
TC19430 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lip... 78 4e-16
TC13156 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lip... 78 5e-16
TC14598 56 2e-09
TC15783 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/F... 40 1e-04
TC11686 weakly similar to UP|Q41126 (Q41126) Non-specific lipid ... 35 0.004
TC10431 similar to UP|Q7XTF6 (Q7XTF6) OJ991214_12.11 protein, pa... 32 0.024
AV417442 32 0.024
AI967893 32 0.041
AI967923 30 0.12
TC15055 weakly similar to UP|O24101 (O24101) MtN5 protein precur... 28 0.35
TC19669 28 0.59
TC14383 homologue to UP|Q8W3Y3 (Q8W3Y3) 1-aminocyclopropane-1-ca... 27 1.0
TC10710 similar to UP|Q9SUZ2 (Q9SUZ2) 5B protein like protein, p... 26 1.7
TC16053 similar to UP|Q96332 (Q96332) Glyoxalase II, partial (85%) 25 5.0
>TC11986 weakly similar to UP|Q8RYA8 (Q8RYA8) Lipid transfer precursor
protein (Nonspecific lipid-transfer protein) (LTP),
partial (77%)
Length = 468
Score = 111 bits (277), Expect = 4e-26
Identities = 54/117 (46%), Positives = 77/117 (65%)
Frame = +2
Query: 1 MSSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDG 60
M++++VL K L ++MCMVLG P AL C QV+ + PC+ Y+ G VP PCC+G
Sbjct: 68 MATTLVL-KFASL-VVMCMVLGSPFAEAALPCGQVQFTVAPCIGYLRSPGPSVPAPCCNG 241
Query: 61 VKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
++ + NQA T +DRQ ACRC+K+ + ++ GLN+ LA LP KCGV+ Y + PS C
Sbjct: 242 IRTLKNQAKTIADRQGACRCLKSTSLSLPGLNLPALASLPGKCGVNFSYKITPSINC 412
>TC14628 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-transfer
protein precursor (LTP), partial (91%)
Length = 604
Score = 97.1 bits (240), Expect = 8e-22
Identities = 49/111 (44%), Positives = 64/111 (57%), Gaps = 1/111 (0%)
Frame = +1
Query: 8 VKVTCLTMMMCMVL-GLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINN 66
VK +C+ + +CM L P A+SC V L PC+PY+TG PQ CC GVK +N
Sbjct: 88 VKFSCMVVAICMALVAAPMAEAAISCGSVIGALTPCMPYLTGGPAPSPQ-CCAGVKNLNA 264
Query: 67 QAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
A T DR+ AC C+K A ++ LN A LP KCGV++PY + ST C
Sbjct: 265 AASTTPDRKTACGCLKNAAGSMPNLNAGNAAALPGKCGVNIPYKISTSTNC 417
>TC8034 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-transfer
protein precursor (LTP), partial (84%)
Length = 742
Score = 95.9 bits (237), Expect = 2e-21
Identities = 45/118 (38%), Positives = 68/118 (57%)
Frame = +1
Query: 2 SSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGV 61
+ M VK C +++ MV+ P A++C + + L+PC Y+ G G P PCC GV
Sbjct: 31 TKQMASVKFVCALVVLSMVVA-PMAEAAITCGAIASALIPCTAYIQGGAGPSP-PCCAGV 204
Query: 62 KAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTCEK 119
+ +N+ A + +DR+AAC C+K+A + N A LP KCGV++PY + ST C K
Sbjct: 205 RRVNSAASSTADRRAACNCLKSAAGSFPRFNAGNAASLPGKCGVNIPYKISTSTNCAK 378
>TC8033 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-transfer
protein precursor (LTP), partial (85%)
Length = 707
Score = 94.4 bits (233), Expect = 5e-21
Identities = 44/116 (37%), Positives = 67/116 (56%)
Frame = +3
Query: 2 SSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGV 61
+ M VK C +++ MV+ P A++C + + L+PC Y+ G G P PCC GV
Sbjct: 63 TKQMASVKFVCALVVLSMVVA-PMAEAAITCGAIASALIPCTAYIQGGAGPSP-PCCAGV 236
Query: 62 KAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
+ +N+ A + +DR+AAC C+K+A + N A LP KCGV++PY + ST C
Sbjct: 237 RRVNSAASSTADRRAACNCLKSAAGSFPRFNAGNAASLPGKCGVNIPYKISTSTNC 404
>TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lipid-transfer
protein 1 precursor (LTP 1), partial (52%)
Length = 683
Score = 93.6 bits (231), Expect = 9e-21
Identities = 43/117 (36%), Positives = 66/117 (55%), Gaps = 4/117 (3%)
Frame = +1
Query: 7 LVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINN 66
LV + + ++ V +P+ A++C QV L PCV Y+ G VP CC+G++ +
Sbjct: 91 LVSLAIVFAVLVAVTTVPRAEGAVTCGQVVNSLYPCVSYLMNGGNMVPGQCCNGIRNLYG 270
Query: 67 QAVTKSDRQAACRCIKTATS----AIHGLNMDILAGLPSKCGVHLPYTLGPSTTCEK 119
A DR+A C CIK A S + N+++ AGLP KCGV++PY + P+T C +
Sbjct: 271 MATNTPDRRAVCTCIKQAVSNSGFSFTSYNLNLAAGLPKKCGVNIPYQISPNTDCSR 441
>TC19050 weakly similar to UP|Q9M6T9 (Q9M6T9) Nonspecific lipid-transfer
protein (LTP), partial (57%)
Length = 579
Score = 89.7 bits (221), Expect = 1e-19
Identities = 47/117 (40%), Positives = 67/117 (57%)
Frame = +2
Query: 1 MSSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDG 60
M++++VL K L +M+ MVLG P AL C Q+ + PC+ Y+ VP CC+G
Sbjct: 26 MAAALVL-KFASLAVMV-MVLGSPFADAALPCGQLTFTVAPCIGYLRNPTPTVPAACCNG 199
Query: 61 VKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
++ I QA T DRQ CRC+K+ + G+N+ LA +P KCG L Y + PS C
Sbjct: 200 IRDILRQAKTVPDRQGVCRCLKSTVYNLPGINLPALASVPGKCGAKLSYKISPSIDC 370
>TC19430 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lipid-transfer
protein 3 precursor (LTP 3) (CW20) (CW-20) (CW-19),
partial (53%)
Length = 520
Score = 78.2 bits (191), Expect = 4e-16
Identities = 35/109 (32%), Positives = 57/109 (52%)
Frame = +3
Query: 11 TCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVT 70
+ + ++ ++L + + A+SC V L PCV Y+ G P CC G KA+ + T
Sbjct: 33 SAFSALVLLMLLVSASEAAISCSDVIKDLKPCVSYLVSGSGKPPGACCSGAKALASAVST 212
Query: 71 KSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTCEK 119
D++AAC CIK+ +I +N + LP CG+++P + P C K
Sbjct: 213 SEDKKAACNCIKSTAKSIK-MNSQLAKALPGNCGINVPINVSPDADCSK 356
>TC13156 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lipid-transfer
protein 3 precursor (LTP 3) (CW20) (CW-20) (CW-19),
partial (53%)
Length = 603
Score = 77.8 bits (190), Expect = 5e-16
Identities = 35/109 (32%), Positives = 57/109 (52%)
Frame = +1
Query: 11 TCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVT 70
+ + ++ ++L + + A+SC V L PCV Y+ G P CC G KA+ + T
Sbjct: 22 SAFSALVLLMLLVSASEAAISCSDVIKDLKPCVSYLVSGSGKPPVACCSGAKALASAVST 201
Query: 71 KSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTCEK 119
D++AAC CIK+ +I +N + LP CG++ P ++ P C K
Sbjct: 202 SEDKKAACNCIKSTAKSIK-MNSQLAEALPGNCGINTPISVSPDADCSK 345
>TC14598
Length = 613
Score = 56.2 bits (134), Expect = 2e-09
Identities = 37/131 (28%), Positives = 61/131 (46%), Gaps = 17/131 (12%)
Frame = +1
Query: 6 VLVKVTCLTMMM--------------CMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGG 51
+L +T LTM M ++L + ++C V + PC ++ N
Sbjct: 10 ILKPITSLTMKMPRTATIFLAGLLPIVILLSISAAQATITCPAVIQAVTPCASFLLQNSS 189
Query: 52 YVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTL 111
Q CCDGVK ++ +A T+ + A C+C+K + + + + ++ LP CGV P TL
Sbjct: 190 -PSQGCCDGVKKLSGEAGTQEGKTAICQCLKQGLAQVGKYDPNRVSELPKDCGV--PLTL 360
Query: 112 GP---STTCEK 119
P +T C K
Sbjct: 361 PPIDQNTDCTK 393
>TC15783 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/F16J14_17),
partial (48%)
Length = 578
Score = 40.0 bits (92), Expect = 1e-04
Identities = 25/98 (25%), Positives = 38/98 (38%), Gaps = 1/98 (1%)
Frame = -2
Query: 12 CLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTK 71
CL M+M + + C T L PC+ Y+TG+ CC + +V +
Sbjct: 418 CLVMVMVATMWTQNAAQS-GCTSALTSLSPCLNYITGSSSSPSPSCCSQL-----SSVVQ 257
Query: 72 SDRQAACRCIKTATSAIH-GLNMDILAGLPSKCGVHLP 108
S Q C + S+ +N + LP C V P
Sbjct: 256 SSPQCLCSLLNGGGSSFGITMNQTLALSLPGPCKVQTP 143
>TC11686 weakly similar to UP|Q41126 (Q41126) Non-specific lipid
transfer-like protein, partial (66%)
Length = 460
Score = 35.0 bits (79), Expect = 0.004
Identities = 25/102 (24%), Positives = 43/102 (41%), Gaps = 1/102 (0%)
Frame = +3
Query: 8 VKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQP-CCDGVKAINN 66
V + + +++ M L + C E +M C P VT P P CC
Sbjct: 57 VTLHLMVVLLMMASTLKVSKGISLCNMNEDGIMACKPSVTKPNPVDPSPECC-------- 212
Query: 67 QAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLP 108
QA+T +D + C ++ + G++ + LP+KC + P
Sbjct: 213 QALTGADLKCLCSYKNSSELPLLGIDPTLAVSLPAKCNLTPP 338
>TC10431 similar to UP|Q7XTF6 (Q7XTF6) OJ991214_12.11 protein, partial (38%)
Length = 582
Score = 32.3 bits (72), Expect = 0.024
Identities = 20/81 (24%), Positives = 32/81 (38%), Gaps = 3/81 (3%)
Frame = +2
Query: 38 KLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILA 97
KL+PC V Q CC V+ I + C + + + + G+N I
Sbjct: 173 KLIPCASAAQDENASVSQSCCAQVQKIG------KNPSCLCAVVLSNMAKMSGVNPKIAI 334
Query: 98 GLPSKCGVH---LPYTLGPST 115
+P +C + + Y GP T
Sbjct: 335 TIPKRCNLDNRPIGYKCGPYT 397
>AV417442
Length = 391
Score = 32.3 bits (72), Expect = 0.024
Identities = 18/72 (25%), Positives = 28/72 (38%), Gaps = 3/72 (4%)
Frame = +3
Query: 35 VETKLMPCVPYVT---GNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIHGL 91
+ T PC+ ++T GNG CCD +K++ T R C + +
Sbjct: 189 ISTSFTPCMSFLTNSSGNGTSPTAECCDSIKSL-----TSGGRDCLCLVVTGNVPFSIPI 353
Query: 92 NMDILAGLPSKC 103
N + LP C
Sbjct: 354 NRTLAISLPRAC 389
>AI967893
Length = 258
Score = 31.6 bits (70), Expect = 0.041
Identities = 12/34 (35%), Positives = 16/34 (46%)
Frame = +3
Query: 31 SCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAI 64
SC V L PC+ Y+TGN CC + +
Sbjct: 141 SCTNVLVSLSPCLNYITGNSSTPSSGCCSQLATV 242
>AI967923
Length = 302
Score = 30.0 bits (66), Expect = 0.12
Identities = 24/96 (25%), Positives = 32/96 (33%), Gaps = 4/96 (4%)
Frame = +3
Query: 34 QVETKLMPCVPYVTGN----GGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIH 89
++ T PC+PYV+ G CCD A T D C ++
Sbjct: 45 ELRTSFSPCLPYVSSPPNNLTGTASTACCDAF-----SAATAVDSLCFCHLLRDPVILGF 209
Query: 90 GLNMDILAGLPSKCGVHLPYTLGPSTTCEKYVYKPH 125
LN L L S C P C + +PH
Sbjct: 210 SLNTTRLLSLSSVC------PSAPRFLCSESPAQPH 299
>TC15055 weakly similar to UP|O24101 (O24101) MtN5 protein precursor,
partial (52%)
Length = 664
Score = 28.5 bits (62), Expect = 0.35
Identities = 27/109 (24%), Positives = 43/109 (38%), Gaps = 2/109 (1%)
Frame = +3
Query: 2 SSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVET-KLMPCVPYVTGNGGYVP-QPCCD 59
SS LV+ + + ++ G A++ ++T +L C TG P + CCD
Sbjct: 45 SSGKALVQWLAAALFIALLGGA----QAVALCNIDTSQLKSCRAAATGEHPPPPDKKCCD 212
Query: 60 GVKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLP 108
V+ N C C + G+N LPS+CG+ P
Sbjct: 213 VVRQAN----------LPCLCKYKSALPSFGINPTQALKLPSECGLSTP 329
>TC19669
Length = 533
Score = 27.7 bits (60), Expect = 0.59
Identities = 22/77 (28%), Positives = 34/77 (43%), Gaps = 10/77 (12%)
Frame = -2
Query: 58 CDGVKAIN---NQAVTKSDRQAACRCIKTATS---AIHGLNMDILAGLPSKCGVH----L 107
C VK ++ NQ +T Q + S HGL ++I+ +PS C + +
Sbjct: 328 CSNVKLLHKHANQLITLLQFQPKDNIVMNCPSWQRI*HGLKINIVISIPSVCDFNTLQPM 149
Query: 108 PYTLGPSTTCEKYVYKP 124
P TLG + T + V P
Sbjct: 148 PPTLGTTRTTKTVVLFP 98
>TC14383 homologue to UP|Q8W3Y3 (Q8W3Y3) 1-aminocyclopropane-1-carboxylic
acid oxidase, complete
Length = 1249
Score = 26.9 bits (58), Expect = 1.0
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2
Query: 58 CDGVKAINNQAVTKSDRQAACRCIK 82
C G K +++TK D ++ C C+K
Sbjct: 923 CQGTKI*GPESITKCDSRSNCHCLK 997
>TC10710 similar to UP|Q9SUZ2 (Q9SUZ2) 5B protein like protein, partial
(58%)
Length = 730
Score = 26.2 bits (56), Expect = 1.7
Identities = 16/67 (23%), Positives = 29/67 (42%), Gaps = 1/67 (1%)
Frame = +1
Query: 38 KLMPCVPYVTGNGGYVP-QPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDIL 96
+L+PC V P + CC+ +K++ C C+ I G++ ++
Sbjct: 16 QLIPCRAAVAPFSPIPPNEACCNALKSLGQP----------CLCVLVNGPPISGVDRNMA 165
Query: 97 AGLPSKC 103
+ LP KC
Sbjct: 166 SLLPEKC 186
>TC16053 similar to UP|Q96332 (Q96332) Glyoxalase II, partial (85%)
Length = 860
Score = 24.6 bits (52), Expect = 5.0
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +3
Query: 42 CVPYVTGNGGYVPQP 56
C+ YVTG+G PQP
Sbjct: 300 CITYVTGDGPDQPQP 344
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.135 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,661,606
Number of Sequences: 28460
Number of extensions: 36356
Number of successful extensions: 181
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of query: 125
length of database: 4,897,600
effective HSP length: 80
effective length of query: 45
effective length of database: 2,620,800
effective search space: 117936000
effective search space used: 117936000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 50 (23.9 bits)
Medicago: description of AC147537.12