BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147497.9 - phase: 0
(148 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, ... 76 3e-15
TC18970 weakly similar to UP|Q8I8F0 (Q8I8F0) Chromosome scaffold... 30 0.22
AV411760 29 0.29
AV418250 29 0.38
AU252214 28 0.50
TC14775 PIR|S14181|S14181 DNA-directed RNA polymerase largest c... 28 0.50
TC8228 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid h... 28 0.65
CB829423 28 0.85
TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g... 28 0.85
BP067133 27 1.1
TC18608 similar to UP|Q9M7D2 (Q9M7D2) Root border cell-specific ... 27 1.1
BP056834 27 1.5
TC12670 similar to GB|AAP68227.1|31711742|BT008788 At5g38640 {Ar... 27 1.5
TC16524 similar to GB|AAL69537.1|18491137|AY074839 AT5g45420/MFC... 27 1.9
AV411498 27 1.9
TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, parti... 26 2.5
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 26 3.2
AU088956 26 3.2
TC19891 homologue to PIR|S14182|S14182 DNA-directed RNA polymera... 26 3.2
CN825239 25 4.2
>TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, partial
(57%)
Length = 1280
Score = 75.9 bits (185), Expect = 3e-15
Identities = 47/117 (40%), Positives = 67/117 (57%), Gaps = 6/117 (5%)
Frame = +1
Query: 32 SSPSYQPKSSKPIP---FSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAIC 88
S P SKP P F++TFD SNP AFL+ VFDF+++ES F ++AEK V S +
Sbjct: 154 SKTDASPSQSKPSPSPTFAATFDRSNPAAFLERVFDFVSRES-DFLATEAAEKEVASLVR 330
Query: 89 TVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEK---EDEKKDEESGLTAPNQGNLM 142
+ K+ K EE++K + A AEK+ K+ +K E+ K E + PN+GN M
Sbjct: 331 AAREKRKK-KREEELKASEEKAKAEKRLKEAEKKPKAEENDKKEGTAAIVPNKGNGM 498
>TC18970 weakly similar to UP|Q8I8F0 (Q8I8F0) Chromosome scaffold protein
p85, partial (4%)
Length = 562
Score = 29.6 bits (65), Expect = 0.22
Identities = 19/49 (38%), Positives = 25/49 (50%), Gaps = 5/49 (10%)
Frame = +3
Query: 85 SAICTVKVKKAKITVEE-----KMKVDKAAAVAEKKAKDVYEKEDEKKD 128
S C VK K K V+E ++ DK V EKK + EK +EKK+
Sbjct: 93 SCFCFVKPKAGKPKVKEVKDKKEVVKDKKEVVKEKKEEAKEEKSEEKKE 239
>AV411760
Length = 420
Score = 29.3 bits (64), Expect = 0.29
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
Frame = +1
Query: 67 AKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAE---KKAKDVYEKE 123
AK+ ++ D+D + + +VKK K EE+ K + VAE K+ K VY+
Sbjct: 175 AKKELNYSDDDDMP-IAKRTTVSKEVKKKKNVKEEEEKQETKKKVAETPKKREKKVYDLP 351
Query: 124 DEKKD 128
+K+D
Sbjct: 352 GQKRD 366
>AV418250
Length = 420
Score = 28.9 bits (63), Expect = 0.38
Identities = 14/26 (53%), Positives = 17/26 (64%)
Frame = +1
Query: 31 TSSPSYQPKSSKPIPFSSTFDPSNPT 56
T SP +PKS+ P+PF TF P N T
Sbjct: 154 TFSPISEPKSN-PLPFPPTFAPKNST 228
>AU252214
Length = 350
Score = 28.5 bits (62), Expect = 0.50
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +1
Query: 101 EKMKVDKAAAVAEKKAKDVYEKEDEKKDEESG 132
E+ + KA+A +EKK K+ EKED+ E G
Sbjct: 103 EEARARKASAESEKKNKETKEKEDQFWKEAEG 198
>TC14775 PIR|S14181|S14181 DNA-directed RNA polymerase largest chain
(isoform B1) - soybean (fragment) {Glycine
max;} , partial (22%)
Length = 848
Score = 28.5 bits (62), Expect = 0.50
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNP 55
+SPSY P S P S T+ PS+P
Sbjct: 220 TSPSYSPTSPSYSPSSPTYSPSSP 291
Score = 27.7 bits (60), Expect = 0.85
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +1
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SP+Y P S P S ++ PS+PT
Sbjct: 199 TSPNYSPTSPSYSPTSPSYSPSSPT 273
>TC8228 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid
hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein) , partial (95%)
Length = 883
Score = 28.1 bits (61), Expect = 0.65
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = +2
Query: 29 TQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKES 70
T TS P S+ PFS T P + A +V+DF K++
Sbjct: 95 TTTSFPIKSLVSTSTTPFSFTLRPDHTMAAPTSVYDFTVKDA 220
>CB829423
Length = 383
Score = 27.7 bits (60), Expect = 0.85
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -3
Query: 33 SPS-YQPKSSKPIPFSSTFDPSNPTAFLKN 61
SPS +QP +PF S F PSN T F K+
Sbjct: 225 SPSQHQPHPLFVVPFQSRFQPSNITQFPKH 136
>TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800),
partial (24%)
Length = 498
Score = 27.7 bits (60), Expect = 0.85
Identities = 20/52 (38%), Positives = 26/52 (49%)
Frame = +2
Query: 79 AEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
A KV ++ +AKI + K KVD +A +K A EK EKK EE
Sbjct: 242 ANKVTVTGKFDAVKLQAKIAEKTKKKVDLVSAPPKKDA-GAGEKSPEKKPEE 394
>BP067133
Length = 507
Score = 27.3 bits (59), Expect = 1.1
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +1
Query: 26 TVKTQTSSPSYQPKSSKPIPFSSTFDPSNPT 56
T T TSSP+ P S P P SS PS P+
Sbjct: 307 TTTTATSSPTASPSSPSPTPASS--PPSAPS 393
>TC18608 similar to UP|Q9M7D2 (Q9M7D2) Root border cell-specific protein,
partial (24%)
Length = 354
Score = 27.3 bits (59), Expect = 1.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -1
Query: 33 SPSYQPKSSKPIPFSSTFDPSNPTAF 58
SPS++ + K +PFS T S+P +F
Sbjct: 336 SPSFRQNAHKALPFSPTRHSSSPNSF 259
>BP056834
Length = 517
Score = 26.9 bits (58), Expect = 1.5
Identities = 15/27 (55%), Positives = 16/27 (58%)
Frame = -3
Query: 33 SPSYQPKSSKPIPFSSTFDPSNPTAFL 59
SPSYQ SKP PFS T+ AFL
Sbjct: 428 SPSYQ--ESKPFPFSYTYSNHAYCAFL 354
>TC12670 similar to GB|AAP68227.1|31711742|BT008788 At5g38640 {Arabidopsis
thaliana;}, partial (28%)
Length = 737
Score = 26.9 bits (58), Expect = 1.5
Identities = 19/51 (37%), Positives = 23/51 (44%), Gaps = 1/51 (1%)
Frame = +1
Query: 79 AEKVVLSAICTVKVKKAKITVEEKMKVDKAA-AVAEKKAKDVYEKEDEKKD 128
AE S TV K V+ KVD A +EKK D ++D KKD
Sbjct: 220 AEGNKASGTTTVMNAKPAKAVKSAQKVDNVTVAASEKKGTDCPPEKDRKKD 372
>TC16524 similar to GB|AAL69537.1|18491137|AY074839 AT5g45420/MFC19_9
{Arabidopsis thaliana;}, partial (41%)
Length = 997
Score = 26.6 bits (57), Expect = 1.9
Identities = 22/59 (37%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = -3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPTAFL--KNVFDFIAKESTSFFDNDSAEKVVLSAIC 88
SSP Q SS P SS+ P AFL KN + ++ S F N + LSA+C
Sbjct: 650 SSPLLQLVSSSTFPSSSSPPPLLSAAFLFFKNC-SYESESSLLFSPNSLDFLITLSALC 477
>AV411498
Length = 419
Score = 26.6 bits (57), Expect = 1.9
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVF 63
SSP+ +P +K S TF P +P F N+F
Sbjct: 378 SSPTIEPAVTK----SCTFSPRHPVFFFNNLF 295
>TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, partial (5%)
Length = 574
Score = 26.2 bits (56), Expect = 2.5
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = -2
Query: 29 TQTSSPSYQPKSSKPIPFSSTFDPSNPTA 57
TQ + PK S+ IPFS PSN TA
Sbjct: 207 TQGKHINGSPKPSE*IPFSLIIPPSNETA 121
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 25.8 bits (55), Expect = 3.2
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = +1
Query: 89 TVKVKKAKITV---EEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTA 135
+V KK K+ V E K+KV+ EKK K KKD+E+G A
Sbjct: 7 SVPAKKVKVDVVVDEGKVKVEVEGEKKEKKKKK-------KKDQENGEVA 135
>AU088956
Length = 330
Score = 25.8 bits (55), Expect = 3.2
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +1
Query: 39 KSSKPIPFSSTFDPSNPTAFL 59
KSS + F + DPSNP FL
Sbjct: 34 KSSNDVLFDAAPDPSNPATFL 96
>TC19891 homologue to PIR|S14182|S14182 DNA-directed RNA polymerase largest
chain (isoform B2) - soybean (fragment)
{Glycine max;} , partial (34%)
Length = 498
Score = 25.8 bits (55), Expect = 3.2
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +3
Query: 31 TSSPSYQPKSSKPIPFSSTFDPSNP 55
TSSP Y P S P S + P++P
Sbjct: 276 TSSPGYSPSSPGYSPSSPGYSPTSP 350
Score = 25.0 bits (53), Expect = 5.5
Identities = 10/24 (41%), Positives = 14/24 (57%)
Frame = +3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNP 55
SSP Y P S P S ++ P++P
Sbjct: 426 SSPGYSPTSPAYSPTSPSYSPTSP 497
Score = 24.3 bits (51), Expect = 9.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SP Y P S P S + P++PT
Sbjct: 342 TSPGYSPTSPGYSPTSPGYSPTSPT 416
>CN825239
Length = 702
Score = 25.4 bits (54), Expect = 4.2
Identities = 14/39 (35%), Positives = 21/39 (52%), Gaps = 6/39 (15%)
Frame = +3
Query: 108 AAAVAEKKAKD------VYEKEDEKKDEESGLTAPNQGN 140
AAA AE++ + + ED+++ EE G APN N
Sbjct: 519 AAAAAEEEGQQHNWIFSADQMEDDEQPEEEGYIAPNLAN 635
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.126 0.336
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,840,447
Number of Sequences: 28460
Number of extensions: 19138
Number of successful extensions: 204
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of query: 148
length of database: 4,897,600
effective HSP length: 82
effective length of query: 66
effective length of database: 2,563,880
effective search space: 169216080
effective search space used: 169216080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC147497.9