BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147497.3 - phase: 0
(382 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU251673 54 3e-08
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 53 1e-07
BI418821 53 1e-07
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 47 5e-06
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 44 3e-05
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 43 8e-05
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 42 2e-04
TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial ... 37 0.007
TC17929 33 0.061
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 32 0.13
AV420911 31 0.30
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 31 0.39
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 31 0.39
TC18767 30 0.51
TC9099 similar to GB|AAO42817.1|28416573|BT004571 At1g27330 {Ara... 28 1.9
TC11457 28 2.5
AV773507 28 2.5
BP065763 28 3.3
BF177636 28 3.3
>AU251673
Length = 413
Score = 54.3 bits (129), Expect = 3e-08
Identities = 35/138 (25%), Positives = 65/138 (46%), Gaps = 1/138 (0%)
Frame = +2
Query: 72 CSEVQKVRFGTHMLAEEADDWWISLLPVLEQDDAVVTWAMFKKEFLGRYFPEDVRGKKKI 131
CS+ + V + L A DW+ L TWA F EF+ R+ P+ VR
Sbjct: 5 CSDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVR 184
Query: 132 EFLELKQGD-MSVTEYAAKFVELATFYPHYSAETAEFSKCIKFENGLRADIKRAIGYQQI 190
+F L+Q + M+V+EY+A F L+ + P+ E + +F GL+ + +++ +
Sbjct: 185 DFERLEQAEGMTVSEYSAHFTHLSRYVPY---PLLEEERVKRFVRGLKEYLFKSVVGSKS 355
Query: 191 RVFYELVNRCRIYQEDTK 208
E+++ + ++ K
Sbjct: 356 STLSEVLSLALLVEQRQK 409
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 52.8 bits (125), Expect = 1e-07
Identities = 28/97 (28%), Positives = 42/97 (42%)
Frame = +2
Query: 215 SERRGKGQQNRPKPYSAPADKGKQKMGDDRRPKRRDAHVEIVCYKCGEKGHKSNVCGRDD 274
S R + + P +D+ + RR RR + +C C GH + C +
Sbjct: 218 SRSRSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECP-NV 394
Query: 275 RKCFRCGQKGHSLAECKRGDIVCYNCNGEGHISSQCP 311
C CG GH +EC + C+NC GH++S CP
Sbjct: 395 AICHNCGLPGHIASECTTKSL-CWNCKEPGHMASSCP 502
>BI418821
Length = 614
Score = 52.8 bits (125), Expect = 1e-07
Identities = 23/69 (33%), Positives = 30/69 (43%), Gaps = 15/69 (21%)
Frame = +2
Query: 257 CYKCGEKGHKSNVCGRDDRK--------CFRCGQKGHSLAECKR-------GDIVCYNCN 301
CY CG+ GH + C R + C+ CG GH +C R G CYNC
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCG 571
Query: 302 GEGHISSQC 310
GH++ C
Sbjct: 572 DTGHLARDC 598
Score = 41.6 bits (96), Expect = 2e-04
Identities = 17/52 (32%), Positives = 21/52 (39%), Gaps = 8/52 (15%)
Frame = +2
Query: 277 CFRCGQKGHSLAECKR--------GDIVCYNCNGEGHISSQCPEPKKTRTGG 320
C+ CG GH +C R G CYNC GH++ C GG
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGG 547
Score = 40.0 bits (92), Expect = 6e-04
Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 7/45 (15%)
Frame = +2
Query: 257 CYKCGEKGHKSNVCGRDDRK-------CFRCGQKGHSLAECKRGD 294
CY CG+ GH + C R + C+ CG GH +C R +
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 47.0 bits (110), Expect = 5e-06
Identities = 21/57 (36%), Positives = 30/57 (51%), Gaps = 5/57 (8%)
Frame = +2
Query: 276 KCFRCGQKGHSLAECKRGD--IVCYNCNGEGHISSQC---PEPKKTRTGGKVFALTG 327
+CF CG GH +CK GD CY C GH+ C P+ + +T ++F+ TG
Sbjct: 368 RCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNEWQTWKELFSFTG 538
Score = 39.7 bits (91), Expect = 8e-04
Identities = 16/37 (43%), Positives = 20/37 (53%), Gaps = 2/37 (5%)
Frame = +2
Query: 257 CYKCGEKGHKSNVCGRDD--RKCFRCGQKGHSLAECK 291
C+ CG GH + C D KC+RCG +GH CK
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCK 481
Score = 27.3 bits (59), Expect = 4.3
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +2
Query: 257 CYKCGEKGHKSNVCGRDDRK 276
CY+CG++GH C +K
Sbjct: 437 CYRCGDRGHVERNCKNSPKK 496
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 44.3 bits (103), Expect = 3e-05
Identities = 29/97 (29%), Positives = 39/97 (39%)
Frame = -2
Query: 218 RGKGQQNRPKPYSAPADKGKQKMGDDRRPKRRDAHVEIVCYKCGEKGHKSNVCGRDDRKC 277
+G G NR P +K QK +RP+ R + G + + C
Sbjct: 500 QGSGGPNRSAPGRFDRNKSFQKK-PFQRPQNRGTSSGY-SHSFGNFVPRPTQSDTSEIVC 327
Query: 278 FRCGQKGHSLAECKRGDIVCYNCNGEGHISSQCPEPK 314
RC +KGH C D+VC+NC GH C PK
Sbjct: 326 HRCSKKGHFANRCP--DLVCWNCQKTGHSGKDCTNPK 222
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 43.1 bits (100), Expect = 8e-05
Identities = 20/43 (46%), Positives = 22/43 (50%), Gaps = 3/43 (6%)
Frame = +1
Query: 276 KCFRCGQKGHSLAECKRGD--IVCYNCNGEGHISSQCP-EPKK 315
+CF CG GH +CK GD CY C GHI C PKK
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKK 516
Score = 40.4 bits (93), Expect = 5e-04
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +1
Query: 257 CYKCGEKGHKSNVCGRDD--RKCFRCGQKGHSLAECK 291
C+ CG GH + C D KC+RCG++GH CK
Sbjct: 391 CFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCK 501
Score = 28.9 bits (63), Expect = 1.5
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +1
Query: 257 CYKCGEKGHKSNVCGRDDRKCFR 279
CY+CGE+GH C +K R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 42.0 bits (97), Expect = 2e-04
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +3
Query: 277 CFRCGQKGHSLAECKRGDIVCYNCNGEGHISSQCP 311
C C Q GH +C ++C+NC G GH++ +CP
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECP 113
Score = 35.4 bits (80), Expect = 0.016
Identities = 15/39 (38%), Positives = 18/39 (45%)
Frame = +3
Query: 255 IVCYKCGEKGHKSNVCGRDDRKCFRCGQKGHSLAECKRG 293
+VC C + GH S C C CG +GH EC G
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSG 119
>TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial (90%)
Length = 1737
Score = 36.6 bits (83), Expect = 0.007
Identities = 23/70 (32%), Positives = 31/70 (43%), Gaps = 10/70 (14%)
Frame = +1
Query: 255 IVCYKCGEKGHKSNVCGRDDRKCFRCGQKGHSLAECKRG-------DIVCYNCNGEGHIS 307
++C KC KG KS GR CF C G + + G VC +C G G +
Sbjct: 583 VLCLKCKGKGSKSGTAGR----CFGCQGTGMKVIRRQIGLGMVQQMQHVCSDCRGTGEVI 750
Query: 308 SQ---CPEPK 314
S+ CP+ K
Sbjct: 751 SERDRCPQCK 780
>TC17929
Length = 791
Score = 33.5 bits (75), Expect = 0.061
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +2
Query: 237 KQKMGDDRRPKRRDAHVEIVCYKCGEKGHKSNVCGRD 273
+ ++G++ + D+ CY+CGE GHK C ++
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKE 112
Score = 27.3 bits (59), Expect = 4.3
Identities = 7/16 (43%), Positives = 11/16 (68%)
Frame = +2
Query: 277 CFRCGQKGHSLAECKR 292
C+RCG+ GH + C +
Sbjct: 62 CYRCGESGHKMRNCPK 109
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 32.3 bits (72), Expect = 0.13
Identities = 26/97 (26%), Positives = 41/97 (41%), Gaps = 13/97 (13%)
Frame = +1
Query: 234 DKGKQKMGDDRRPKRRDA----------HVEIVCYKCGEKGHKSNVCGR--DDRKCFRCG 281
++ +++ + R + RDA VE+ G G + GR +D KC+ CG
Sbjct: 1 ERERERERERERERERDAIHALDGKNGWRVELSHNSKGGGGGRGGGRGRGGEDLKCYECG 180
Query: 282 QKGHSLAECK-RGDIVCYNCNGEGHISSQCPEPKKTR 317
+ GH EC+ RG G G + P P + R
Sbjct: 181 EPGHFARECRSRG-----GSRGLGSGRRRSPSPYRRR 276
>AV420911
Length = 418
Score = 31.2 bits (69), Expect = 0.30
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 271 GRDDRKCFRCGQKGHSLAECK 291
G +D KC+ CG+ GH EC+
Sbjct: 356 GGEDLKCYECGEPGHFARECR 418
Score = 26.6 bits (57), Expect = 7.4
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 254 EIVCYKCGEKGHKSNVC 270
++ CY+CGE GH + C
Sbjct: 365 DLKCYECGEPGHFAREC 415
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 30.8 bits (68), Expect = 0.39
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 271 GRDDRKCFRCGQKGHSLAECK 291
G D KC+ CG+ GH EC+
Sbjct: 361 GGSDMKCYECGEPGHFARECR 423
Score = 26.2 bits (56), Expect = 9.7
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +1
Query: 254 EIVCYKCGEKGHKSNVC 270
++ CY+CGE GH + C
Sbjct: 370 DMKCYECGEPGHFAREC 420
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 30.8 bits (68), Expect = 0.39
Identities = 17/58 (29%), Positives = 25/58 (42%)
Frame = +2
Query: 262 EKGHKSNVCGRDDRKCFRCGQKGHSLAECKRGDIVCYNCNGEGHISSQCPEPKKTRTG 319
E+G + G DR G +G + EC + C GH + +CP +TR G
Sbjct: 335 ERGRDRDDRGDRDRSRGYGGSRGSNGGEC-------FKCGKPGHFARECPSEGETRRG 487
>TC18767
Length = 1004
Score = 30.4 bits (67), Expect = 0.51
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +2
Query: 273 DDRKCFRCGQKGHSLAECKR 292
D +CF CG HSL EC R
Sbjct: 155 DASRCFNCGSYNHSLRECSR 214
>TC9099 similar to GB|AAO42817.1|28416573|BT004571 At1g27330 {Arabidopsis
thaliana;}, complete
Length = 518
Score = 28.5 bits (62), Expect = 1.9
Identities = 18/68 (26%), Positives = 32/68 (46%), Gaps = 4/68 (5%)
Frame = +2
Query: 212 KIMSERRGKGQQNRPKPYSAPADKGKQKMGDDRRPKRRDAHVEIVCYKCG----EKGHKS 267
+I S+R G +R P S GD P+++ +++ C + G E+ HK
Sbjct: 68 RIGSDRNGTPHHHRLPPLS----------GDPLSPQQKHDNIKAPCRQEGGEV*EEHHKE 217
Query: 268 NVCGRDDR 275
+C R++R
Sbjct: 218 RICARNNR 241
>TC11457
Length = 629
Score = 28.1 bits (61), Expect = 2.5
Identities = 15/47 (31%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = -2
Query: 272 RDDRKCFRCGQKGHSLAECKRGDIVCYNCNGEGHIS-SQCPEPKKTR 317
RD ++ G++G S A+C+R + CN E H+ +C + TR
Sbjct: 526 RDPVWLYKPGREGVSRAKCQRIPNGTHGCNREAHVPVDRCRSHRDTR 386
>AV773507
Length = 496
Score = 28.1 bits (61), Expect = 2.5
Identities = 26/89 (29%), Positives = 37/89 (41%), Gaps = 2/89 (2%)
Frame = +2
Query: 234 DKGKQKMGDDRRPKRRDAHVEIVCYKCGEKGH--KSNVCGRDDRKCFRCGQKGHSLAECK 291
+K + KMG D D Y G +G + G+DD CF CG+ GH +C
Sbjct: 233 NKAQPKMGGDDA----DQGYRGGGYSSGGRGSYGAGDRVGQDD--CFECGRPGHRARDCP 394
Query: 292 RGDIVCYNCNGEGHISSQCPEPKKTRTGG 320
G G +SS+ P+ +GG
Sbjct: 395 LAGGGGGRGRGGGSLSSR---PRYGGSGG 472
>BP065763
Length = 537
Score = 27.7 bits (60), Expect = 3.3
Identities = 20/62 (32%), Positives = 24/62 (38%), Gaps = 9/62 (14%)
Frame = -1
Query: 262 EKGHKSNVCGRDDR-----KCFRCGQKGHSLAECKRGDI----VCYNCNGEGHISSQCPE 312
EK H ++ D K R QK + K G I VCY C GH CP+
Sbjct: 513 EKDHSIHLTTHDGASXSKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCPK 334
Query: 313 PK 314
K
Sbjct: 333 RK 328
Score = 26.6 bits (57), Expect = 7.4
Identities = 13/40 (32%), Positives = 17/40 (42%)
Frame = -1
Query: 231 APADKGKQKMGDDRRPKRRDAHVEIVCYKCGEKGHKSNVC 270
A + +QK + K H E VCY C + GH C
Sbjct: 459 AKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYC 340
>BF177636
Length = 483
Score = 27.7 bits (60), Expect = 3.3
Identities = 20/86 (23%), Positives = 33/86 (38%), Gaps = 3/86 (3%)
Frame = +1
Query: 167 FSKCIKFENGLRADIKRAIGYQQIRVFYELVNRCRIYQEDTKAHYKIMSERRG---KGQQ 223
F K K + RAD+ A + +F+E++N+ Q D + K + K +
Sbjct: 127 FGKADKQDRAGRADLSTAKTFYAASIFFEILNQFGAVQPDLEQKQKYAVWKAADIRKALK 306
Query: 224 NRPKPYSAPADKGKQKMGDDRRPKRR 249
KP + P D + P R
Sbjct: 307 EGRKPVAGPPDGDEDLSVPSSTPSNR 384
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.137 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,523,357
Number of Sequences: 28460
Number of extensions: 95058
Number of successful extensions: 502
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 435
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 473
length of query: 382
length of database: 4,897,600
effective HSP length: 92
effective length of query: 290
effective length of database: 2,279,280
effective search space: 660991200
effective search space used: 660991200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC147497.3