BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147496.8 + phase: 1 /partial
(120 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV425443 133 9e-33
TC14402 similar to UP|Q8L9D9 (Q8L9D9) Beta-1,3-glucanase-like pr... 102 1e-23
TC10642 weakly similar to UP|Q84V39 (Q84V39) Allergen Ole e 10, ... 84 6e-18
TC12989 similar to GB|AAO39944.1|28372924|BT003716 At5g08000 {Ar... 83 1e-17
TC14813 similar to UP|Q9M4A9 (Q9M4A9) Beta-1,3 glucanase precurs... 81 5e-17
TC8065 similar to GB|AAP12844.1|30017221|BT006195 At1g18650 {Ara... 76 1e-15
TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-... 73 1e-14
AV768339 70 1e-13
TC13502 62 3e-11
TC10737 weakly similar to UP|E13B_WHEAT (P52409) Glucan endo-1,3... 62 3e-11
TC12695 similar to UP|Q9SAK1 (Q9SAK1) T8K14.10, partial (17%) 60 1e-10
TC14528 similar to UP|Q8L9D9 (Q8L9D9) Beta-1,3-glucanase-like pr... 60 1e-10
TC17674 similar to GB|CAB79832.1|7270016|ATCHRIV74 1, 3-beta-glu... 59 2e-10
TC11230 weakly similar to GB|BAB10263.1|10177025|AB025632 beta-1... 53 1e-08
TC10988 weakly similar to UP|Q94EN5 (Q94EN5) Beta-1,3-glucanase ... 50 1e-07
CB828957 43 2e-05
TC16512 similar to UP|Q9FXL4 (Q9FXL4) Elicitor inducible chitina... 42 4e-05
TC11182 similar to UP|O23562 (O23562) Glucanase homolog (Glucana... 42 4e-05
TC18663 similar to UP|Q94EN5 (Q94EN5) Beta-1,3-glucanase (Fragm... 41 5e-05
BP057569 38 5e-04
>AV425443
Length = 372
Score = 133 bits (334), Expect = 9e-33
Identities = 62/108 (57%), Positives = 76/108 (69%), Gaps = 5/108 (4%)
Frame = +3
Query: 4 PSSTVPITAP-----STSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQP 58
PS +VP+ P +T+N P + G SWC+A A Q SLQ ALDYACG G DC+A+Q
Sbjct: 51 PSGSVPVINPQPPPANTNNPPSTQGQSWCVAKTGAPQASLQAALDYACGKGA-DCAALQQ 227
Query: 59 GGSCYNPNSVHDHASFAFNKYYQKNPVPNSCNFGGNAVLTNTNPSKAS 106
GGSCY+P ++ +HAS+AFN YYQKNP P SC+FGG A L NTNPS S
Sbjct: 228 GGSCYSPVTLQNHASYAFNSYYQKNPAPTSCDFGGAATLVNTNPSSGS 371
>TC14402 similar to UP|Q8L9D9 (Q8L9D9) Beta-1,3-glucanase-like protein,
partial (20%)
Length = 642
Score = 102 bits (255), Expect = 1e-23
Identities = 46/92 (50%), Positives = 60/92 (65%), Gaps = 1/92 (1%)
Frame = +1
Query: 23 GASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQK 82
G +WC+A+ S+ LQ ALDYACG GG DCSAIQPG +CY+PN++ HASFAFN YYQK
Sbjct: 49 GRTWCVANGGYSEEKLQAALDYACGEGGADCSAIQPGATCYDPNTLEAHASFAFNSYYQK 228
Query: 83 NP-VPNSCNFGGNAVLTNTNPSKASTIYDHGF 113
+C+FGG A + P + + G+
Sbjct: 229 KARGTGTCDFGGAAYVVTQAPKYGTCEFPTGY 324
>TC10642 weakly similar to UP|Q84V39 (Q84V39) Allergen Ole e 10, partial
(65%)
Length = 603
Score = 84.0 bits (206), Expect = 6e-18
Identities = 38/87 (43%), Positives = 53/87 (60%), Gaps = 1/87 (1%)
Frame = +2
Query: 24 ASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKN 83
+ WC A AS+ +LQ +D+AC G DC I+ GG C+ PN+V HASFA N YYQ +
Sbjct: 2 SKWCTAKQGASEAALQANIDFACSTKGVDCGPIKQGGPCFKPNTVRAHASFAMNAYYQAS 181
Query: 84 PVPNS-CNFGGNAVLTNTNPSKASTIY 109
++ C+FG V+T+ NPS + Y
Sbjct: 182 GRHDTDCDFGHTGVVTSINPSYETCTY 262
>TC12989 similar to GB|AAO39944.1|28372924|BT003716 At5g08000 {Arabidopsis
thaliana;}, partial (41%)
Length = 430
Score = 83.2 bits (204), Expect = 1e-17
Identities = 40/84 (47%), Positives = 55/84 (64%), Gaps = 1/84 (1%)
Frame = +2
Query: 21 SSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYY 80
SS ASWC+ ++ SLQ ALDYACG G DC+ I+ G CYNPN+V H ++A N Y+
Sbjct: 176 SSSASWCVCK-DGNEASLQKALDYACG-AGADCNPIKQTGPCYNPNTVRAHCNYAVNSYF 349
Query: 81 Q-KNPVPNSCNFGGNAVLTNTNPS 103
Q K P +C+F G A ++ ++PS
Sbjct: 350 QKKGQAPLACDFAGAATVSASDPS 421
>TC14813 similar to UP|Q9M4A9 (Q9M4A9) Beta-1,3 glucanase precursor ,
partial (21%)
Length = 550
Score = 80.9 bits (198), Expect = 5e-17
Identities = 37/86 (43%), Positives = 52/86 (60%), Gaps = 1/86 (1%)
Frame = +3
Query: 19 PVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNK 78
P + G WC+ AS ++LQ ++Y C DC IQPGG+C+ PN V A++A N
Sbjct: 24 PAAGGRKWCVPKADASNQALQANINYVCSQN-VDCKPIQPGGTCFAPNDVRALATYAMNA 200
Query: 79 YYQKNPVPN-SCNFGGNAVLTNTNPS 103
YYQ N + +C+F AV+T+TNPS
Sbjct: 201 YYQANGRHDYNCDFSHTAVITSTNPS 278
>TC8065 similar to GB|AAP12844.1|30017221|BT006195 At1g18650 {Arabidopsis
thaliana;}, partial (50%)
Length = 1204
Score = 76.3 bits (186), Expect = 1e-15
Identities = 37/90 (41%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +3
Query: 21 SSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYY 80
++ A+WCI AS LQ LDYACG G DC+ + G C+ PN+V H S+A N ++
Sbjct: 255 ANAATWCICK-DASDAILQKTLDYACG-AGADCNPLHTNGPCFQPNTVRAHCSYAVNSFF 428
Query: 81 Q-KNPVPNSCNFGGNAVLTNTNPSKASTIY 109
Q K +C+F G A +NPS S +Y
Sbjct: 429 QKKGQGQGTCDFAGTATAITSNPSIGSCVY 518
>TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like protein,
partial (83%)
Length = 1784
Score = 72.8 bits (177), Expect = 1e-14
Identities = 45/139 (32%), Positives = 60/139 (42%), Gaps = 39/139 (28%)
Frame = +1
Query: 13 PSTSNSPV-SSGASWCIASPS-------------------------------------AS 34
P+T +P S+GASWC+A AS
Sbjct: 1126 PTTPVTPAPSAGASWCVAKQGVSDAQLQAHLDYVCSQSHIDCGPIPVQELHGVNLKQGAS 1305
Query: 35 QRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKNPVPN-SCNFGG 93
LQ LDY C DC IQ GG+C+ P++V HASFA N YYQ + + +C+F
Sbjct: 1306 DAQLQANLDYVCSQSNIDCGPIQSGGACFEPDTVASHASFAMNLYYQTSGRNSWNCDFSQ 1485
Query: 94 NAVLTNTNPSKASTIYDHG 112
+L + NPS + IY G
Sbjct: 1486 TGMLVSQNPSYNACIYSAG 1542
>AV768339
Length = 576
Score = 69.7 bits (169), Expect = 1e-13
Identities = 34/73 (46%), Positives = 47/73 (63%), Gaps = 3/73 (4%)
Frame = -3
Query: 43 DYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAVLTNTN 101
D+ACG G DCS + G C+ P++V HA++AFN YYQK P +C+F G A +T+T+
Sbjct: 574 DWACGPGKVDCSPLLQGQPCHEPDNVVAHATYAFNAYYQKMAKSPGTCDFKGVAAITSTD 395
Query: 102 PSKASTIY--DHG 112
PS S I+ HG
Sbjct: 394 PSHGSCIFPGSHG 356
>TC13502
Length = 517
Score = 62.0 bits (149), Expect = 3e-11
Identities = 31/88 (35%), Positives = 42/88 (47%), Gaps = 1/88 (1%)
Frame = +2
Query: 21 SSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYY 80
+ G WC A+ L AL AC G C A+ PG C+ P S+ DHAS+ F+ Y+
Sbjct: 53 TKGKVWCXGPKEANLMELAAALSNACNEGNIACDALAPGKECHEPASITDHASYVFSSYW 232
Query: 81 QK-NPVPNSCNFGGNAVLTNTNPSKAST 107
+ SC F G A T +P S+
Sbjct: 233 SRFRSEGASCYFNGLAKQTTPDPPGQSS 316
>TC10737 weakly similar to UP|E13B_WHEAT (P52409) Glucan
endo-1,3-beta-glucosidase precursor ((1->3)-beta-glucan
endohydrolase) ((1->3)-beta-glucanase)
(Beta-1,3-endoglucanase) , partial (15%)
Length = 607
Score = 61.6 bits (148), Expect = 3e-11
Identities = 32/91 (35%), Positives = 52/91 (56%), Gaps = 1/91 (1%)
Frame = +2
Query: 20 VSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKY 79
V+ +WC+A PS+ Q +L ++YAC + DC +Q G C +P+ + + AS A N Y
Sbjct: 152 VNGQKTWCVAKPSSDQATLLSNINYACAH--VDCQIMQRGCPCSSPDRLINRASIAMNIY 325
Query: 80 YQ-KNPVPNSCNFGGNAVLTNTNPSKASTIY 109
YQ K +C+F + ++ T+PS + IY
Sbjct: 326 YQSKGRNHWNCDFRASGLVVVTDPSYGNCIY 418
>TC12695 similar to UP|Q9SAK1 (Q9SAK1) T8K14.10, partial (17%)
Length = 571
Score = 60.1 bits (144), Expect = 1e-10
Identities = 28/53 (52%), Positives = 35/53 (65%)
Frame = +1
Query: 41 ALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKNPVPNSCNFGG 93
A+DYACG G DC +QP G C+ PN+V HAS+AFN Y+Q N+ N GG
Sbjct: 1 AMDYACG-SGADCKLVQPNGPCFQPNTVLAHASYAFNSYFQ-----NTKNGGG 141
>TC14528 similar to UP|Q8L9D9 (Q8L9D9) Beta-1,3-glucanase-like protein,
partial (13%)
Length = 599
Score = 59.7 bits (143), Expect = 1e-10
Identities = 28/59 (47%), Positives = 35/59 (58%), Gaps = 1/59 (1%)
Frame = +1
Query: 56 IQPGGSCYNPNSVHDHASFAFNKYYQKNP-VPNSCNFGGNAVLTNTNPSKASTIYDHGF 113
IQPG +CY+PNS+ HASFAFN YYQK SC FGG + + P S + G+
Sbjct: 7 IQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEPKYGSCEFPTGY 183
>TC17674 similar to GB|CAB79832.1|7270016|ATCHRIV74 1, 3-beta-glucanase-like
protein {Arabidopsis thaliana;} , partial (21%)
Length = 850
Score = 58.9 bits (141), Expect = 2e-10
Identities = 38/98 (38%), Positives = 59/98 (59%), Gaps = 4/98 (4%)
Frame = +1
Query: 26 WCIASPSASQRSLQVA--LDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKN 83
WCI PSA+ + Q+A + YAC + DC+++ G SC N + +H + S+AFN Y+Q N
Sbjct: 70 WCIMKPSANLNNDQLAPSVSYACSH--ADCTSLGYGTSCGNLD-IHGNISYAFNSYFQIN 240
Query: 84 -PVPNSCNF-GGNAVLTNTNPSKASTIYDHGFKLQSDT 119
+ ++C F GG +V+T+ +PS I D FK+ T
Sbjct: 241 DQIDSACKFPGGLSVVTDRDPS----IGDCKFKIMIQT 342
>TC11230 weakly similar to GB|BAB10263.1|10177025|AB025632
beta-1,3-glucanase-like {Arabidopsis thaliana;} ,
partial (15%)
Length = 574
Score = 53.1 bits (126), Expect = 1e-08
Identities = 25/53 (47%), Positives = 36/53 (67%), Gaps = 1/53 (1%)
Frame = +3
Query: 52 DCSAIQPGGSCYNPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAVLTNTNPS 103
DC+A+ PGGSC+N S + S+AFN YYQ+ + SC+FGG ++T +PS
Sbjct: 27 DCTALSPGGSCFN-ISWPSNISYAFNSYYQQHDQTAESCDFGGLGLITTVDPS 182
>TC10988 weakly similar to UP|Q94EN5 (Q94EN5) Beta-1,3-glucanase (Fragment)
, partial (21%)
Length = 521
Score = 49.7 bits (117), Expect = 1e-07
Identities = 22/46 (47%), Positives = 31/46 (66%), Gaps = 1/46 (2%)
Frame = +2
Query: 62 CYNPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAVLTNTNPSKAS 106
CY P++V HAS+AF+ YY K P+SC+F G A +T ++PS S
Sbjct: 8 CYEPDNVISHASYAFDSYYHKMGNTPDSCDFKGVATITTSDPSHGS 145
>CB828957
Length = 489
Score = 42.7 bits (99), Expect = 2e-05
Identities = 29/77 (37%), Positives = 41/77 (52%), Gaps = 2/77 (2%)
Frame = +2
Query: 26 WCIASPSASQRS-LQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQ-KN 83
WC+ +P A S L ++YAC + DC+A+ G SC N + +AS+AFN Y+Q +N
Sbjct: 17 WCMFNPDAKDLSKLADNINYACTFA--DCTALGYGSSC-NHLDANGNASYAFNSYFQVQN 187
Query: 84 PVPNSCNFGGNAVLTNT 100
P F G L T
Sbjct: 188 QDPWPAIFKG*LSLLQT 238
>TC16512 similar to UP|Q9FXL4 (Q9FXL4) Elicitor inducible chitinase
Nt-SubE76, partial (48%)
Length = 857
Score = 41.6 bits (96), Expect = 4e-05
Identities = 20/42 (47%), Positives = 25/42 (58%), Gaps = 7/42 (16%)
Frame = +2
Query: 13 PSTSNSPVSSGAS-------WCIASPSASQRSLQVALDYACG 47
P S +PVS G S WC+A+P A + LQ ALD+ACG
Sbjct: 731 PVPSPAPVSGGVSKSTTGNNWCVANPDAEKVKLQAALDFACG 856
>TC11182 similar to UP|O23562 (O23562) Glucanase homolog (Glucanase like
protein), partial (14%)
Length = 566
Score = 41.6 bits (96), Expect = 4e-05
Identities = 25/70 (35%), Positives = 35/70 (49%), Gaps = 1/70 (1%)
Frame = +3
Query: 52 DCSAIQPGGSCYNPNSVHDHASFAFNKYYQ-KNPVPNSCNFGGNAVLTNTNPSKASTIYD 110
DC+ + GGSC N + S+AFN YYQ + SCNF G ++T +PS +
Sbjct: 33 DCTTLNYGGSC-NEIGEKGNVSYAFNSYYQLRMQDSQSCNFDGLGMITFLDPSVGDCRFL 209
Query: 111 HGFKLQSDTT 120
G S T+
Sbjct: 210 VGVGSDSSTS 239
>TC18663 similar to UP|Q94EN5 (Q94EN5) Beta-1,3-glucanase (Fragment) ,
partial (18%)
Length = 494
Score = 41.2 bits (95), Expect = 5e-05
Identities = 19/40 (47%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = +1
Query: 71 HASFAFNKYY-QKNPVPNSCNFGGNAVLTNTNPSKASTIY 109
HA++AF+ YY Q P SCNF A +T +NPS S I+
Sbjct: 7 HANYAFDTYYHQMGKSPESCNFNQMATITTSNPSHGSCIF 126
>BP057569
Length = 587
Score = 37.7 bits (86), Expect = 5e-04
Identities = 18/40 (45%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = -1
Query: 71 HASFAFNKYYQ-KNPVPNSCNFGGNAVLTNTNPSKASTIY 109
HA++AFN YYQ + +C+F G A +T TNPS S +
Sbjct: 587 HATYAFNAYYQLMDKSSGTCDFKGVASVTTTNPSHGSCTF 468
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.125 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,483,614
Number of Sequences: 28460
Number of extensions: 38852
Number of successful extensions: 287
Number of sequences better than 10.0: 101
Number of HSP's better than 10.0 without gapping: 269
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 272
length of query: 120
length of database: 4,897,600
effective HSP length: 79
effective length of query: 41
effective length of database: 2,649,260
effective search space: 108619660
effective search space used: 108619660
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 49 (23.5 bits)
Medicago: description of AC147496.8