BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147482.3 - phase: 0
(175 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17556 similar to UP|Q39131 (Q39131) Lamin (AT5G15350/F8M21_240... 117 8e-28
TC13215 similar to UP|Q39040 (Q39040) Lamin, partial (15%) 100 2e-22
BP046587 67 1e-12
AU089318 65 5e-12
TC10483 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial (... 59 5e-10
TC19996 weakly similar to UP|ENL1_ARATH (Q9SK27) Early nodulin-l... 59 5e-10
TC12474 weakly similar to UP|O82576 (O82576) Blue copper protein... 59 5e-10
TC16136 similar to UP|Q9ZRV5 (Q9ZRV5) Basic blue copper protein,... 59 5e-10
TC17588 similar to UP|BCP_PEA (Q41001) Blue copper protein precu... 58 8e-10
TC14275 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial (... 57 1e-09
TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-li... 57 2e-09
AV407087 49 5e-07
TC12447 weakly similar to GB|AAM63773.1|21555085|AY086719 phytoc... 49 6e-07
TC12369 weakly similar to UP|ENL1_ARATH (Q9SK27) Early nodulin-l... 46 3e-06
TC14908 weakly similar to PIR|A86218|A86218 protein T27G7.18 [im... 39 6e-04
AV764731 37 0.002
AV409301 35 0.005
AV422171 32 0.047
BP067721 27 2.6
TC13140 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger pro... 26 3.3
>TC17556 similar to UP|Q39131 (Q39131) Lamin (AT5G15350/F8M21_240), partial
(68%)
Length = 595
Score = 117 bits (294), Expect = 8e-28
Identities = 66/147 (44%), Positives = 86/147 (57%), Gaps = 8/147 (5%)
Frame = +2
Query: 1 MKNSRVLLMVSVVLGVLSLWPMVVMGGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWL 60
M S ++++ + VL + P V K VG K W NVNYT W+ +H Y+ DWL
Sbjct: 95 MLGSPAMVLLFLGFAVLLMVPEV---SAKRWLVGDGKFWNPNVNYTVWARDKHFYIDDWL 265
Query: 61 KFVFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRG-GRDVVQLTEAKTYYFITGGGYCFHG 119
FV+D+ NVLEVNKT Y+ CI I N T G GRDVV L + YYFI+G G+C+ G
Sbjct: 266 FFVYDRNQMNVLEVNKTNYENCIAEHPIHNWTTGAGRDVVPLNVTRHYYFISGNGFCYGG 445
Query: 120 MKVAVDVQEHPTP-------APSPSLS 139
MK+AV V++ P P A +PSLS
Sbjct: 446 MKLAVRVEKLPPPPKAAPEKAGAPSLS 526
>TC13215 similar to UP|Q39040 (Q39040) Lamin, partial (15%)
Length = 452
Score = 99.8 bits (247), Expect = 2e-22
Identities = 48/107 (44%), Positives = 64/107 (58%)
Frame = +2
Query: 31 HKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRN 90
H VG ++GW NYT W++ + YVGD++ F + K YNV EVN+TGYD C N
Sbjct: 140 HIVGANRGWNPGQNYTLWANNQTFYVGDFISFRYQKNQYNVFEVNQTGYDNCTTEGAFGN 319
Query: 91 LTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDVQEHPTPAPSPS 137
+ G+D + L + +YFI G G CF+GMKV+V V HP AP S
Sbjct: 320 YS-SGKDFIMLNKTGRHYFICGNGQCFNGMKVSVVV--HPLAAPPTS 451
>BP046587
Length = 502
Score = 67.4 bits (163), Expect = 1e-12
Identities = 41/112 (36%), Positives = 59/112 (52%), Gaps = 3/112 (2%)
Frame = -3
Query: 62 FVFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMK 121
F + K YNV EVN+TGYD C N + G+D + L + +YFI G G CF+GMK
Sbjct: 458 FRYQKNQYNVFEVNQTGYDNCTTEGAFGNYS-SGKDFIMLNKTGRHYFICGNGQCFNGMK 282
Query: 122 VAVDVQEHPTPAPSPSLS---DTAKSGGDSILPSMYTCFGIIVANVVYVSLV 170
V+V V HP AP S + T KS +L + ++++N+ + V
Sbjct: 281 VSVVV--HPLAAPPTSSTGEHSTPKSSAPVVLER--GLWSLLLSNLAWFGFV 138
>AU089318
Length = 641
Score = 65.5 bits (158), Expect = 5e-12
Identities = 48/166 (28%), Positives = 77/166 (45%), Gaps = 12/166 (7%)
Frame = +3
Query: 8 LMVSVVLGVLSLWPMVVMGGPKLHKVGGSKGWK--ENVNYTTWSSQEHVYVGDWLKFVFD 65
L+ V+ V+ + V + +HKVG S GW NV+Y W++ + VGD + F ++
Sbjct: 39 LVQRAVVLVMVVMAAVQVSYAAVHKVGDSAGWTILGNVDYKKWAAPXNFQVGDTIIFEYN 218
Query: 66 KRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAV 124
+++NV+ V Y C + I + G D +++T ++F G G+C G KV +
Sbjct: 219 AQFHNVMRVTHAMYXTCNASSPIATFST-GNDSIKITNHGHHFFFCGVPGHCQAGQKVDI 395
Query: 125 DV---------QEHPTPAPSPSLSDTAKSGGDSILPSMYTCFGIIV 161
+V PTPAPS S A + PS +IV
Sbjct: 396 NVISVSAATAAAPTPTPAPSSPSSSLATPSANVPAPSPNNATPLIV 533
>TC10483 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial (24%)
Length = 552
Score = 58.9 bits (141), Expect = 5e-10
Identities = 41/120 (34%), Positives = 54/120 (44%), Gaps = 11/120 (9%)
Frame = +3
Query: 31 HKVGGSKGWKENVN----YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMT 86
H VG S GW YT W++ + VGD L F F +NV +V K+ +D C +
Sbjct: 168 HVVGDSTGWTIPTGGASFYTNWAANKTFSVGDTLVFNFANGQHNVAKVTKSAFDSCSGSS 347
Query: 87 FIRNLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDV------QEHPTPAPSPSLS 139
+ L G V LTE +FI GG+C G K+AV P P P+P S
Sbjct: 348 PV-TLISTGPATVTLTETGVQHFICAFGGHCSAGQKLAVTXVAGKASNNAPAPVPAPKSS 524
>TC19996 weakly similar to UP|ENL1_ARATH (Q9SK27) Early nodulin-like protein
1 precursor (Phytocyanin-like protein), partial (36%)
Length = 458
Score = 58.9 bits (141), Expect = 5e-10
Identities = 37/102 (36%), Positives = 54/102 (52%)
Frame = +3
Query: 9 MVSVVLGVLSLWPMVVMGGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRY 68
MV+ +L +L +P V H VG + GW V+YTTW+S + VGD L F +
Sbjct: 87 MVASLLVLLVAFPTVFGAD---HTVGDASGWNIGVDYTTWASGKTFKVGDNLVFTYSSSL 257
Query: 69 YNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFI 110
+ V EV+++ Y C + I+ + G V LT+A T YFI
Sbjct: 258 HGVDEVDESSYKSCSTSSPIKTYSDGNTKVA-LTKAGTLYFI 380
>TC12474 weakly similar to UP|O82576 (O82576) Blue copper protein
(Fragment), partial (44%)
Length = 818
Score = 58.9 bits (141), Expect = 5e-10
Identities = 45/131 (34%), Positives = 62/131 (46%), Gaps = 6/131 (4%)
Frame = +1
Query: 31 HKVGGSKGWKENVN---YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
H VG S GW N YT+W+++ VGD L F + +NV EV KT YD C +
Sbjct: 157 HVVGDSAGWFVPGNTSFYTSWAAKNTFAVGDTLVFNYAASAHNVEEVTKTNYDSCNSTSP 336
Query: 88 IRNLTRGGRDVVQLTEAKT--YYFITG-GGYCFHGMKVAVDVQEHPTPAPSPSLSDTAKS 144
I T V +T KT +YFI G G+C K++++V + A PS + T S
Sbjct: 337 IATYT---TPPVTVTIKKTGAHYFICGVPGHCLGDQKLSINVASGSSAATPPSSTATPPS 507
Query: 145 GGDSILPSMYT 155
+ P T
Sbjct: 508 SSTTPPPPSST 540
>TC16136 similar to UP|Q9ZRV5 (Q9ZRV5) Basic blue copper protein, partial
(82%)
Length = 872
Score = 58.9 bits (141), Expect = 5e-10
Identities = 36/119 (30%), Positives = 62/119 (51%), Gaps = 1/119 (0%)
Frame = +1
Query: 8 LMVSVVLGVLSLWPMVVM-GGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDK 66
++V+V + +L L + V + VGG GW N + TW + + GD L F +D
Sbjct: 106 VVVTVGISLLCLLALQVEHANAATYTVGGPAGWSFNTD--TWPNGKKFRAGDVLIFNYDS 279
Query: 67 RYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVD 125
+NV+ V+++GY C + L+ G+D ++L + Y+ G+C GMKVA++
Sbjct: 280 TTHNVVAVDQSGYKSCTTPAGAKVLS-SGKDQIRLGRGQNYFICNCPGHCQSGMKVAIN 453
>TC17588 similar to UP|BCP_PEA (Q41001) Blue copper protein precursor,
partial (61%)
Length = 747
Score = 58.2 bits (139), Expect = 8e-10
Identities = 50/163 (30%), Positives = 77/163 (46%), Gaps = 20/163 (12%)
Frame = +1
Query: 30 LHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIR 89
++ VG + GW +Y+TW+S + VGD L F + + V EV ++ Y C +
Sbjct: 10 VYSVGDTSGWAAGADYSTWTSDKTFAVGDSLVFNYGAG-HTVDEVKESDYKSCTTGNSL- 183
Query: 90 NLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDVQ---EHPTPAP---SPSLSD-- 140
+ G+ + L A T+YFI G+C GMK+AV V+ TP+ SPS +
Sbjct: 184 STDSSGKTTIALKTAGTHYFICSVPGHCSGGMKLAVTVKGAASFTTPSSGKGSPSTTTGT 363
Query: 141 -----------TAKSGGDSILPSMYTCFGIIVANVVYVSLVLV 172
T S GDS+ P + +IV+ + Y +L LV
Sbjct: 364 PTSTTTTPPALTDSSSGDSLSPFVAM---LIVSWIFYYALPLV 483
>TC14275 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial (25%)
Length = 562
Score = 57.4 bits (137), Expect = 1e-09
Identities = 43/130 (33%), Positives = 63/130 (48%), Gaps = 3/130 (2%)
Frame = +1
Query: 45 YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEA 104
Y TW+S + VGD L F + +NV EV K YD C + I T V++T
Sbjct: 4 YRTWASGKTFKVGDILVFNYAASAHNVEEVTKQKYDSCNSTSPIATYT---TPPVRVTIK 174
Query: 105 KT--YYFITG-GGYCFHGMKVAVDVQEHPTPAPSPSLSDTAKSGGDSILPSMYTCFGIIV 161
KT +YFI G G+C G K++++V + A PS + + S S P + G +
Sbjct: 175 KTGAHYFICGVPGHCLGGQKLSINVTGGSSTATPPSSASPSPSPSSSTTPPQDSAAGSLG 354
Query: 162 ANVVYVSLVL 171
A V+ S V+
Sbjct: 355 AAGVFASTVM 384
>TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-like protein
2 precursor (Phytocyanin-like protein), partial (31%)
Length = 1482
Score = 56.6 bits (135), Expect = 2e-09
Identities = 41/128 (32%), Positives = 59/128 (46%), Gaps = 9/128 (7%)
Frame = +3
Query: 33 VGGSKGWKENVN--YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRN 90
VGGSKGW N + YT W+ + + D + F + K +VLEV K +D C I+
Sbjct: 219 VGGSKGWIPNPSESYTLWAGRNRFQIKDTIVFKYTKGSDSVLEVKKEDFDNCNKANPIKK 398
Query: 91 LTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDV------QEHPTPAPSPSLSDTAK 143
G + + +YFI+G G C G K+ + V Q P+P P S S + K
Sbjct: 399 FEDGDTEFT-FDRSGPFYFISGKDGNCEKGQKMILVVISPRGTQPPPSPPPKSSPSPSPK 575
Query: 144 SGGDSILP 151
+ S P
Sbjct: 576 ASPPSGSP 599
>AV407087
Length = 363
Score = 48.9 bits (115), Expect = 5e-07
Identities = 28/90 (31%), Positives = 42/90 (46%), Gaps = 1/90 (1%)
Frame = +3
Query: 31 HKVGGSKG-WKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIR 89
H VGG G W N +W + + VGD L F + + NV+EV K YD C + I+
Sbjct: 96 HIVGGPNGGWDATSNLQSWGTSQQFSVGDNLIFQYQPNH-NVIEVTKADYDSCQATSPIQ 272
Query: 90 NLTRGGRDVVQLTEAKTYYFITGGGYCFHG 119
+ + G + + K Y+ G+C G
Sbjct: 273 SYSDGATTIPLSSPGKRYFICGAIGHCTQG 362
>TC12447 weakly similar to GB|AAM63773.1|21555085|AY086719
phytocyanin-related protein-like {Arabidopsis thaliana;}
, partial (56%)
Length = 735
Score = 48.5 bits (114), Expect = 6e-07
Identities = 43/154 (27%), Positives = 70/154 (44%), Gaps = 4/154 (2%)
Frame = +3
Query: 3 NSRVLLMVSVVLGVLSLWPMVVMGGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKF 62
+S LL++ ++ G + ++V G KV S+ N W+ + VGD+L +
Sbjct: 60 SSASLLLLFLLFGFSAAKELLVGGKTDAWKVPSSEADSLN----QWAEKSRFKVGDYLVW 227
Query: 63 VFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGG-GYCFHGMK 121
+D +VL+VNK Y C I + G V+L +YFI+G G+C G K
Sbjct: 228 KYDGGKDSVLQVNKEDYGSCNTSNPIEEY-KDGNTKVKLDRPGPHYFISGAKGHCEKGQK 404
Query: 122 VAVDV---QEHPTPAPSPSLSDTAKSGGDSILPS 152
+ V V + A SP+ S G ++ P+
Sbjct: 405 LFVVVMTPKHSRDRAISPASSPAELEEGPAVAPT 506
>TC12369 weakly similar to UP|ENL1_ARATH (Q9SK27) Early nodulin-like protein
1 precursor (Phytocyanin-like protein), partial (47%)
Length = 574
Score = 46.2 bits (108), Expect = 3e-06
Identities = 37/125 (29%), Positives = 53/125 (41%), Gaps = 26/125 (20%)
Frame = +1
Query: 48 WSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTY 107
W+ ++ +GD L + +D +VLEV+K Y+ C + I+ G V+L +
Sbjct: 19 WAQRDRFQIGDHLVWKYDGGKDSVLEVSKEDYEKCNISSPIKEY-NDGNTKVKLDHPGPF 195
Query: 108 YFITGG-GYCFHGMKVAVDVQ-------------------------EHPTPAPSPSLSDT 141
YFI+G G+C G K+ V V E P APSP S T
Sbjct: 196 YFISGAKGHCEKGQKLVVVVLSPRGGRNTGTDVSPAPASAPVPTGFEGPAVAPSPVSSAT 375
Query: 142 AKSGG 146
A GG
Sbjct: 376 ALQGG 390
>TC14908 weakly similar to PIR|A86218|A86218 protein T27G7.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(68%)
Length = 1219
Score = 38.5 bits (88), Expect = 6e-04
Identities = 38/160 (23%), Positives = 63/160 (38%), Gaps = 33/160 (20%)
Frame = +2
Query: 29 KLHKVGGSKGWKEN-----VNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYC- 82
K + VG S GW +N VNY W + + +GD+L F D + V N T Y C
Sbjct: 197 KNYTVGDSLGWFDNLEKPHVNYQKWVANKEFSLGDFLIFNTDTNHTVVQTYNFTTYKQCD 376
Query: 83 ------IDMTFIRNLTRGGRDVVQLT-------EAKTYYFIT--GGGYCFHGMKVAVDV- 126
D T + ++ +T E Y+F + G C +G ++V
Sbjct: 377 YNDAQDKDTTQWSSSDPSNTEIHPVTAAVPLVKEGMNYFFSSDYDGDQCKNGQHFKINVT 556
Query: 127 -----------QEHPTPAPSPSLSDTAKSGGDSILPSMYT 155
+P+P+ +S + D+I+PS ++
Sbjct: 557 YGQGLPKSLRSPSEDSPSPASPVSGDDDAAPDTIVPSNFS 676
>AV764731
Length = 166
Score = 37.0 bits (84), Expect = 0.002
Identities = 18/52 (34%), Positives = 25/52 (47%)
Frame = +2
Query: 31 HKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYC 82
+ VGG+ GW N W + D L++ F +NV+ VNK YD C
Sbjct: 2 YTVGGAGGW--TFNTVGWPKGKRFRAADTLEYKFGAGAHNVVAVNKAAYDTC 151
>AV409301
Length = 360
Score = 35.4 bits (80), Expect = 0.005
Identities = 20/50 (40%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = +2
Query: 33 VGGSKGWKENV--NYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYD 80
VGG GW N NY W+ + V D L F + +VL VNK YD
Sbjct: 209 VGGKDGWVLNPSENYNQWAGRNRFQVNDNLVFKYKTGSDSVLVVNKEDYD 358
>AV422171
Length = 417
Score = 32.3 bits (72), Expect = 0.047
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -3
Query: 93 RGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVD 125
R G D ++LT + Y+ G+C GMK+A++
Sbjct: 388 RSGNDQIRLTRGQNYFICNYVGHCESGMKIAIN 290
>BP067721
Length = 410
Score = 26.6 bits (57), Expect = 2.6
Identities = 11/29 (37%), Positives = 18/29 (61%)
Frame = -3
Query: 39 WKENVNYTTWSSQEHVYVGDWLKFVFDKR 67
WK++ +T ++EHV + +WL F F R
Sbjct: 240 WKQSQTPSTTLTKEHVLLRNWLGFGFKIR 154
>TC13140 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger protein 12
(LIM domain interacting RING finger protein) (RING
finger LIM domain-binding protein) (R-LIM) (NY-REN-43
antigen), partial (4%)
Length = 526
Score = 26.2 bits (56), Expect = 3.3
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = +3
Query: 112 GGGYCFHGMKVAVDVQEHPTPAPSP-SLSDTAKSGGDSILPS 152
GGG K + P+P+PSP S+S +A S S+L S
Sbjct: 93 GGGGVGINNKRKLQETSDPSPSPSPPSISSSAVSSASSLLDS 218
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.140 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,663,942
Number of Sequences: 28460
Number of extensions: 57011
Number of successful extensions: 426
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 416
length of query: 175
length of database: 4,897,600
effective HSP length: 84
effective length of query: 91
effective length of database: 2,506,960
effective search space: 228133360
effective search space used: 228133360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC147482.3