BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147482.11 - phase: 0
(109 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12925 similar to PIR|S38958|S38958 chorismate mutase precurso... 176 7e-46
TC14425 similar to PIR|T50796|T50796 chorismate mutase CM2 - Ara... 118 1e-28
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 26 1.1
TC17360 similar to UP|AAR92257 (AAR92257) At5g19340, partial (34%) 24 3.3
TC13340 similar to UP|Q94A50 (Q94A50) AT3g55070/T15C9_70, partia... 24 4.3
TC14632 homologue to UP|Q947H2 (Q947H2) Ribosomal protein, parti... 23 5.6
BP039650 23 7.4
BP085462 23 9.6
TC8586 UP|RPOA_LOTJA (Q9BBQ4) DNA-directed RNA polymerase alpha ... 23 9.6
>TC12925 similar to PIR|S38958|S38958 chorismate mutase precursor -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (28%)
Length = 381
Score = 176 bits (445), Expect = 7e-46
Identities = 85/100 (85%), Positives = 96/100 (96%)
Frame = +1
Query: 10 KFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLK 69
+FVAEAKF+A+P+ YKAAIIAQDKD+LM+LLTYPEVEE+IKRRV +KAKTYGQEV VN+K
Sbjct: 13 RFVAEAKFRASPNDYKAAIIAQDKDKLMDLLTYPEVEEAIKRRVEMKAKTYGQEVVVNMK 192
Query: 70 DQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
+ +TEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD
Sbjct: 193 EHRTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 312
>TC14425 similar to PIR|T50796|T50796 chorismate mutase CM2 - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (58%)
Length = 1144
Score = 118 bits (296), Expect = 1e-28
Identities = 59/109 (54%), Positives = 80/109 (73%)
Frame = +2
Query: 1 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
A+S+RIHYGKFVAEAKF+ +P Y+ I A+D + LM+LLT+ V+E + +RV KA +
Sbjct: 566 AISRRIHYGKFVAEAKFRESPQDYEPLIRAKDTEGLMKLLTFESVQEMVIKRVEKKAMVF 745
Query: 61 GQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
GQEV +N D+K E K +PS+ + LY WI+PLTKEV+V YLLR+LD
Sbjct: 746 GQEVGLNSDDEK-EVKRKFDPSVASSLYQKWIIPLTKEVEVEYLLRRLD 889
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 25.8 bits (55), Expect = 1.1
Identities = 20/72 (27%), Positives = 34/72 (46%), Gaps = 7/72 (9%)
Frame = +3
Query: 21 PDSYKAAIIAQ-----DKDRLMELLTYPEVEE--SIKRRVAVKAKTYGQEVAVNLKDQKT 73
PDS AA+ + D ++ L V+E S+ + VKA + + + KDQ
Sbjct: 309 PDSCIAAVSSSNDHPTDPQSILTLSLRASVDELASVASSLGVKASSSNGQGVADCKDQVD 488
Query: 74 EPVYKINPSLVA 85
+ + ++N SL A
Sbjct: 489 DALSRLNDSLSA 524
>TC17360 similar to UP|AAR92257 (AAR92257) At5g19340, partial (34%)
Length = 574
Score = 24.3 bits (51), Expect = 3.3
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +3
Query: 80 NPSLVADLYSDWIMPLTK 97
N ++ +DLY+ WI+P T+
Sbjct: 510 NDNVNSDLYNHWIVPFTQ 563
>TC13340 similar to UP|Q94A50 (Q94A50) AT3g55070/T15C9_70, partial (35%)
Length = 466
Score = 23.9 bits (50), Expect = 4.3
Identities = 17/58 (29%), Positives = 25/58 (42%)
Frame = +2
Query: 49 IKRRVAVKAKTYGQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLR 106
+ R +K K +A +L+ QK S AD S+W K + V Y+LR
Sbjct: 290 VSRLQGLKRKLEEGSLAEHLQAQKCRARIDHLESADADNLSEWNNTRLKRILVDYMLR 463
>TC14632 homologue to UP|Q947H2 (Q947H2) Ribosomal protein, partial (95%)
Length = 772
Score = 23.5 bits (49), Expect = 5.6
Identities = 13/53 (24%), Positives = 24/53 (44%)
Frame = +1
Query: 12 VAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEV 64
VA K+ PD+ + A+ D + E ++++ KAK Y ++V
Sbjct: 55 VAMVKYSREPDNPTKSCKARGSDLRVHFKNTRETAHALRKLPLAKAKRYLEDV 213
>BP039650
Length = 488
Score = 23.1 bits (48), Expect = 7.4
Identities = 18/75 (24%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +2
Query: 18 QAAPDSYKAAIIA-QDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLKDQKTEPV 76
Q +Y A+ + ++++ L+ P + SI + V +++++ D KT+
Sbjct: 62 QCCLHTYMTAMFGCSENNKVVRFLSNPRSQISILQISVVL-------ISIHVLDGKTKFS 220
Query: 77 YKINPSLVADLYSDW 91
+ L+ADLYS W
Sbjct: 221 RW*D*KLIADLYSSW 265
>BP085462
Length = 452
Score = 22.7 bits (47), Expect = 9.6
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = -2
Query: 7 HYGKFVAEAKFQAAPDSYKAA 27
H G+FVAEA+ + +Y+ A
Sbjct: 307 HRGRFVAEARCCGSKSTYRLA 245
>TC8586 UP|RPOA_LOTJA (Q9BBQ4) DNA-directed RNA polymerase alpha chain
(PEP) (Plastid-encoded RNA polymerase alpha subunit)
(RNA polymerase alpha subunit) , partial (79%)
Length = 856
Score = 22.7 bits (47), Expect = 9.6
Identities = 13/72 (18%), Positives = 33/72 (45%), Gaps = 4/72 (5%)
Frame = +2
Query: 3 SKRIHYGKFVAEAKFQAAPDSYKAA----IIAQDKDRLMELLTYPEVEESIKRRVAVKAK 58
SKR++YG+F+ + D+ A ++ + + + + ++ V ++
Sbjct: 137 SKRLYYGRFILSPLMKGQADTIGIAMRRILLGEMEGTCITRAKFEKISHEYSTIVGIQEP 316
Query: 59 TYGQEVAVNLKD 70
+ E+ +NLK+
Sbjct: 317 VH--EILMNLKE 346
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,273,587
Number of Sequences: 28460
Number of extensions: 10095
Number of successful extensions: 53
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 52
length of query: 109
length of database: 4,897,600
effective HSP length: 85
effective length of query: 24
effective length of database: 2,478,500
effective search space: 59484000
effective search space used: 59484000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)
Medicago: description of AC147482.11