BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147471.4 + phase: 0
(274 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU251432 134 2e-47
TC18077 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial... 182 5e-47
AW720503 134 4e-38
TC10406 similar to UP|Q94A21 (Q94A21) AT5g15330/F8M21_220, parti... 150 2e-37
TC16968 weakly similar to UP|Q940E0 (Q940E0) IDS-4-like protein ... 90 5e-19
TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%) 27 4.9
TC11044 26 6.4
AV422598 26 8.4
>AU251432
Length = 364
Score = 134 bits (337), Expect(2) = 2e-47
Identities = 68/84 (80%), Positives = 74/84 (87%)
Frame = +2
Query: 42 SSCSKRRRLDDDGGAEGEVTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVA 101
SS KRRR D+D GA GEV+KEV DFLRLLEVEIEKFN FFVE EEEYVIKWKELQ+KVA
Sbjct: 116 SSSLKRRRSDNDDGA-GEVSKEVNDFLRLLEVEIEKFNAFFVEMEEEYVIKWKELQNKVA 292
Query: 102 WAKSSDIELMTVGREIVDFHGEMV 125
WAK+SD++LM VGREIVD HGEMV
Sbjct: 293 WAKNSDVDLMPVGREIVDLHGEMV 364
Score = 71.2 bits (173), Expect(2) = 2e-47
Identities = 34/38 (89%), Positives = 35/38 (91%)
Frame = +1
Query: 6 ILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSS 43
IL+NQIEQTLPDWRDKFLSYKDLKKQLKLI PKE SS
Sbjct: 1 ILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPKEPSSS 114
>TC18077 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (51%)
Length = 995
Score = 182 bits (462), Expect = 5e-47
Identities = 114/266 (42%), Positives = 153/266 (56%), Gaps = 15/266 (5%)
Frame = +1
Query: 16 PDWRDKFLSYKDLKKQLKLI--VPKEIDSSCSKRRRLDDDGGAEGEVTKEVKDFLRLLEV 73
P+W+D +LSYK+LKK ++LI P ++ S + G AE E F+ LL
Sbjct: 1 PEWKDMYLSYKELKKLVRLISAAPTLLNGSL-------EYGKAEAE-------FMYLLNN 138
Query: 74 EIEKFNGFFVEKEEEYVIKWKELQDKVA-----WAKSSDI--------ELMTVGREIVDF 120
EI+KFNGFF+EKEE+++I+ KE+Q ++ W + E+ + + IVDF
Sbjct: 139 EIDKFNGFFMEKEEDFIIRHKEVQQRIQIILDLWGPNGSQPSEADYKEEMAKIRKAIVDF 318
Query: 121 HGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECE 180
HGEMVLL NYS +NYTGL KI+KKYDKRTG LLRLPFIQ VL QPFF ++++KLV+ECE
Sbjct: 319 HGEMVLLVNYSTINYTGLAKILKKYDKRTGCLLRLPFIQKVLEQPFFTTELISKLVRECE 498
Query: 181 VMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMENIFIKL 240
++ +FP EE A A E + + E IF
Sbjct: 499 SIIDTVFPA--------------EEEAERVKEATE------------AIVVAGEGIF-SY 597
Query: 241 TTSALDTLKEIRGGSSTVSIYSLPPL 266
T +AL T+ +IR GSST S +SLPPL
Sbjct: 598 TVAALLTMHQIRNGSSTESPFSLPPL 675
>AW720503
Length = 539
Score = 134 bits (337), Expect(2) = 4e-38
Identities = 71/124 (57%), Positives = 92/124 (73%), Gaps = 1/124 (0%)
Frame = +2
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEI-DSSCSKRRRLDDDGGAEGE 59
MKF K L++QIE+TLP+WRDKFLSYK+LKK+LK P + +KR RLD G+
Sbjct: 98 MKFGKSLSSQIEKTLPEWRDKFLSYKELKKKLKNFEPAAGGEDRPAKRLRLD----VAGD 265
Query: 60 VTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIELMTVGREIVD 119
++KE DF LLE E++KFN FFVEKEEEY+I+ KELQD+VA K+S ++M + +EIVD
Sbjct: 266 MSKEETDFRNLLENELDKFNNFFVEKEEEYIIRLKELQDRVAKVKASSEQMMKIRKEIVD 445
Query: 120 FHGE 123
FHGE
Sbjct: 446 FHGE 457
Score = 40.0 bits (92), Expect(2) = 4e-38
Identities = 18/21 (85%), Positives = 21/21 (99%)
Frame = +3
Query: 124 MVLLENYSALNYTGLVKIIKK 144
MVLLENYSALNYTGLVKI+++
Sbjct: 459 MVLLENYSALNYTGLVKILEE 521
>TC10406 similar to UP|Q94A21 (Q94A21) AT5g15330/F8M21_220, partial (57%)
Length = 986
Score = 150 bits (379), Expect = 2e-37
Identities = 84/213 (39%), Positives = 126/213 (58%), Gaps = 13/213 (6%)
Frame = +3
Query: 67 FLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDI-------------ELMTV 113
FLR+L E+EKFN F+V+KEEE+VI+++EL++++ K E+M +
Sbjct: 6 FLRILNQELEKFNDFYVDKEEEFVIRFQELKERIERLKDKSYQREMYTFDYEFSEEMMEI 185
Query: 114 GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLN 173
+++V HGEMVLL+NYS+LN+ GL+KI+KKYDKRTG LL+LPF Q VL QPFF + L
Sbjct: 186 RKDLVTIHGEMVLLKNYSSLNFAGLIKILKKYDKRTGGLLQLPFTQLVLRQPFFTTEPLT 365
Query: 174 KLVKECEVMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNM 233
+LV ECE L ++FP + QS S E A + T + +P+ S + +
Sbjct: 366 RLVHECEENLELLFPLEAEVVQSTSLPEHESRPAVDNAT--------NALPETSSNLGDE 521
Query: 234 ENIFIKLTTSALDTLKEIRGGSSTVSIYSLPPL 266
+ T +A+ +K ++ SST + +S L
Sbjct: 522 PMYLYRSTLAAMRAIKGLQKASSTCNRFSFSSL 620
>TC16968 weakly similar to UP|Q940E0 (Q940E0) IDS-4-like protein (Fragment),
partial (19%)
Length = 568
Score = 89.7 bits (221), Expect = 5e-19
Identities = 46/57 (80%), Positives = 50/57 (87%)
Frame = +3
Query: 217 KETLDHVPKEFSEIQNMENIFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 273
+ETL VPKE +EI NMEN+FIKLTTSALDTLKEIRGGSSTVSIYSLPP H E +VE
Sbjct: 6 RETLKQVPKELAEIPNMENMFIKLTTSALDTLKEIRGGSSTVSIYSLPP-HKEAMVE 173
>TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%)
Length = 547
Score = 26.6 bits (57), Expect = 4.9
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = +1
Query: 29 KKQLKLIVPKEIDSSCSKRRRLDDDGGAEGE 59
KK K++VPK+ +SS + D+D +E +
Sbjct: 451 KKPTKVVVPKKEESSSDEDSSSDEDSSSESD 543
>TC11044
Length = 514
Score = 26.2 bits (56), Expect = 6.4
Identities = 15/46 (32%), Positives = 23/46 (49%), Gaps = 5/46 (10%)
Frame = +2
Query: 76 EKFNGFFVEKEEEYVIKWKELQDKVAW-----AKSSDIELMTVGRE 116
E N F +E + +W EL ++AW A++SD+ M RE
Sbjct: 23 EIINNFSQVDDEGPLHQWNELNQRLAWLHSMEAQTSDLLAMRSNRE 160
>AV422598
Length = 475
Score = 25.8 bits (55), Expect = 8.4
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = -3
Query: 243 SALDTLKEIRGGSSTVSIYSLPP 265
S L TL IRGGS S+Y PP
Sbjct: 413 SLLPTLSIIRGGSLIPSLYISPP 345
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.136 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,018,646
Number of Sequences: 28460
Number of extensions: 43949
Number of successful extensions: 230
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of query: 274
length of database: 4,897,600
effective HSP length: 89
effective length of query: 185
effective length of database: 2,364,660
effective search space: 437462100
effective search space used: 437462100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC147471.4