BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147435.7 + phase: 0
(1667 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 228 3e-65
BP084332 191 6e-49
AV780009 130 1e-30
BP052632 105 8e-23
BP075943 79 6e-15
BP057233 70 4e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 69 5e-12
BP063694 65 1e-10
AV766665 63 4e-10
BP043850 62 6e-10
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 47 1e-09
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 61 2e-09
AV427921 59 6e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 59 6e-09
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 58 1e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 57 2e-08
AU088980 55 7e-08
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 55 9e-08
AV424544 48 1e-05
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 35 1e-05
>BP066094
Length = 532
Score = 228 bits (582), Expect(3) = 3e-65
Identities = 110/149 (73%), Positives = 132/149 (87%)
Frame = +2
Query: 1245 ARLEAIRLLLSYAINHGIILYQMDVKSAFLNGVIEEEVYVKQPPGFEDLKHPNHVYKLKK 1304
++ EAIRLL+S+++NH IIL+QM+VKSAFLNG I EEVYV QPPG ED K+ +H++KLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1305 SLYGLKQAPRAWYDRLSNFLIKNDFERGQVDTTLFRRTLKKDILIVQIYVDDIIFGSTNA 1364
SLYGLKQAPRAWY+RLS+FL++N+ RG+VDTTLF +T K DILIVQIYVDDIIFGS N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1365 SLCKEFSKLMQDEFEMSMMGELKFFLGIQ 1393
SLCKEFS++MQ EFEM MMGELK+FLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 3e-65
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1229 YSQQEGIDYTETFAPVAR 1246
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 3e-65
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1219 RNKARLVAQG 1228
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 191 bits (486), Expect = 6e-49
Identities = 89/112 (79%), Positives = 104/112 (92%)
Frame = +2
Query: 1549 QATIAMSTAEAEYISAANCCTQLLWMKHQLEDYQINANSIPIYCDNTAAICLSKNPILHS 1608
Q+TIA+STAEAEYISAA C TQ+LWMKHQLEDYQI ++IPIYCDNTAAI LSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1609 RAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFDFIKKNL 1660
RAKHIE+K+HFIRDYVQKG+L ++F+DT+HQWADIFTKPL+ +RF+FI KNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>AV780009
Length = 529
Score = 130 bits (328), Expect = 1e-30
Identities = 64/163 (39%), Positives = 99/163 (60%), Gaps = 1/163 (0%)
Frame = -1
Query: 1486 AVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISWA 1545
A R+ RY+KG GL + KL + D+D+AG R+S +G FLG +LISW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1546 SKKQATIAMSTAEAEYISAANCCTQLLWMKHQLEDYQINAN-SIPIYCDNTAAICLSKNP 1604
+KKQ T++ S++EAEY + A ++ W+ + + ++N +P++CDN +A+ ++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 1605 ILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKP 1647
H R KHIE+ H +R +Q G++ + I T HQ ADIFTKP
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 105 bits (261), Expect = 8e-23
Identities = 57/124 (45%), Positives = 75/124 (59%), Gaps = 1/124 (0%)
Frame = -3
Query: 963 KTAYELFKGRRPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNS 1022
KT YEL++GRRP +SYFH FGC +ILNT D + KFD K + I LGYS SKAY V+NS
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 1023 ETQCVEESMHVKFDDREPESK-TSEQSESNAGTTDSEDASESDQPSDSEKYTEVESSPEA 1081
TQ VEES++VKFDD E+ E+ N +D ++ +++ SE E S+
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEEPSTQPQ 128
Query: 1082 EITP 1085
P
Sbjct: 127 TTNP 116
>BP075943
Length = 547
Score = 79.0 bits (193), Expect = 6e-15
Identities = 47/157 (29%), Positives = 78/157 (48%), Gaps = 1/157 (0%)
Frame = -3
Query: 1352 IYVDDIIFGSTNASLCKEFSKLMQDEFEMSMMGELKFFLGIQINQSKEGVYVHQTKYTKE 1411
+YVDD++ + + + D+F + +GE K+FLG++I +S G+ ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1412 LLKKFKLEDCKVMNTPMHPTCTLSKEDTGT-VVDQKLYRGMIGSLLYLTASRPDILFSVC 1470
L+ + +TGT + D YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAVN 156
Query: 1471 LCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYRKS 1507
++F S P + H + +YL G+ GL Y S
Sbjct: 155 QLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPAS 45
>BP057233
Length = 473
Score = 69.7 bits (169), Expect = 4e-12
Identities = 32/73 (43%), Positives = 48/73 (64%)
Frame = -2
Query: 1590 IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLS 1649
++CDN +A L+ NP+LH+R+KHIEI H+IRD V + + + ++ T Q AD TKPLS
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1650 VERFDFIKKNLNM 1662
RF ++ L +
Sbjct: 265 HTRFSQLRDKLGV 227
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 69.3 bits (168), Expect = 5e-12
Identities = 33/91 (36%), Positives = 53/91 (57%), Gaps = 1/91 (1%)
Frame = +1
Query: 1573 WMKHQLEDYQINANSIP-IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDI 1631
W+ + L+D ++ +YCDN +A ++ NP+ H R KHIEI H +R+ +QKG++ +
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1632 QFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1662
I + ADI+TK LS + F I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP063694
Length = 511
Score = 64.7 bits (156), Expect = 1e-10
Identities = 40/116 (34%), Positives = 58/116 (49%), Gaps = 3/116 (2%)
Frame = -2
Query: 751 VWHKRLGHANWRLISKISK---LQLVKGLPNIDYHSDALCGACQKGKIVKSSFKSKDIVS 807
+WH RLGH N+ +I ++ K L + LP C +CQ K + F +
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNN-KR 199
Query: 808 TSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSF 863
S L+L+H DL GP AS+ G Y ++ VDD+SR+TW +K K +V F
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRF 31
>AV766665
Length = 601
Score = 62.8 bits (151), Expect = 4e-10
Identities = 34/76 (44%), Positives = 44/76 (57%)
Frame = +3
Query: 1485 TAVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISW 1544
T RIF YLK + G L++K + GF ADY G ++R ST G FL NL++W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1545 ASKKQATIAMSTAEAE 1560
SK+Q IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>BP043850
Length = 515
Score = 62.4 bits (150), Expect = 6e-10
Identities = 27/93 (29%), Positives = 55/93 (59%), Gaps = 1/93 (1%)
Frame = -1
Query: 1569 TQLLWMKHQLEDYQ-INANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKG 1627
++++W++ L + + + P++ DNT+AI ++ NP+ H +HIE+ H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1628 ILDIQFIDTEHQWADIFTKPLSVERFDFIKKNL 1660
++ + + T Q ADI TK L+ +R +F+ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 46.6 bits (109), Expect(2) = 1e-09
Identities = 23/51 (45%), Positives = 33/51 (64%)
Frame = +3
Query: 1612 HIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1662
+IE K+HF+RD V KG + ++ T+ Q ADI TK L ERF ++ LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 34.3 bits (77), Expect(2) = 1e-09
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1593 DNTAAICLSKNPILHSRAKHIE 1614
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.8 bits (146), Expect = 2e-09
Identities = 36/145 (24%), Positives = 74/145 (50%), Gaps = 9/145 (6%)
Frame = +2
Query: 1322 NFLIKNDFERGQVD-TTLFRRTLKKDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEFEM 1380
+F++ + R D T +R D +I+ +YVDD++ N +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1381 SMMGELKFFLGIQINQSKEG--VYVHQTKYTKELLKKFKLEDCKVMNTPMHPTCTLSKED 1438
+G LG+QI++ ++ +++ Q Y +++L++F ++DC ++TP+ LS
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1439 TGTVVDQKL------YRGMIGSLLY 1457
+ +++ Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>AV427921
Length = 284
Score = 58.9 bits (141), Expect = 6e-09
Identities = 32/79 (40%), Positives = 43/79 (53%)
Frame = +1
Query: 909 TPQQNGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYEL 968
TPQQNGV ERKNRT+ M R+++ + L K F EAV S +I NR + E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 969 FKGRRPNISYFHQFGCTCY 987
+ GR+P + +F C Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 58.9 bits (141), Expect = 6e-09
Identities = 35/116 (30%), Positives = 63/116 (54%), Gaps = 3/116 (2%)
Frame = +3
Query: 1542 ISWASKKQATIAMSTAEAEYISAANCCTQLLWMKHQLEDYQINANSIPIYC---DNTAAI 1598
++W S+ Q +A+STAEAE+I+A C +LLWMK+ L++ +++ PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSH--PILCCIVITKALF 200
Query: 1599 CLSKNPILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFD 1654
L++ + + +RD + +L+++ I T+ AD+ TK L E+ +
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLE 368
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 58.2 bits (139), Expect = 1e-08
Identities = 44/148 (29%), Positives = 68/148 (45%), Gaps = 6/148 (4%)
Frame = -1
Query: 1405 QTKYTKELLKKFKLEDCKVMNTPM-HPTCTLSKEDTGTVVDQKLYRGMIGSLLYLTASRP 1463
Q Y ++LKKFK+ + K ++T + + + G VD Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1464 DILFSVCLCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYR-----KSLDYKLIGFCDA 1518
DI+ V L +RF +P + H +R RY+KGT G+ SLD +++
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLDTQIV----- 398
Query: 1519 DYAGDRIERKSTSGNCQFLGENLISWAS 1546
+ G LG ISW+S
Sbjct: 397 ----------TRPGYAFHLGRGAISWSS 344
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 57.4 bits (137), Expect = 2e-08
Identities = 30/81 (37%), Positives = 48/81 (59%), Gaps = 1/81 (1%)
Frame = +3
Query: 1570 QLLWMKHQLEDYQI-NANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGI 1628
+ LW+ + L D +I + + IYCDN +A+ L+ N + H R ++IEI H + V GI
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1629 LDIQFIDTEHQWADIFTKPLS 1649
L + + + Q AD+FTK +S
Sbjct: 201 LHLLHVPSSDQVADVFTKTIS 263
>AU088980
Length = 360
Score = 55.5 bits (132), Expect = 7e-08
Identities = 29/112 (25%), Positives = 52/112 (45%)
Frame = +2
Query: 913 NGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYELFKGR 972
N +VERK+R + + R ++ + L K+ W AV + Y+ N + + + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 973 RPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNSET 1024
P + FG CY +K D +A++ +FLG+ + Y V + T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 55.1 bits (131), Expect = 9e-08
Identities = 29/94 (30%), Positives = 51/94 (53%), Gaps = 1/94 (1%)
Frame = +1
Query: 1570 QLLWMKHQLEDYQINANSIPI-YCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGI 1628
+L W+ + L+D ++ S + YCD+ +A ++ N + H R KH++I H +R+ +Q +
Sbjct: 4 EL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKL 183
Query: 1629 LDIQFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1662
+ I + Q ADI TKPL F + L +
Sbjct: 184 FHLLPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>AV424544
Length = 276
Score = 48.1 bits (113), Expect = 1e-05
Identities = 23/90 (25%), Positives = 49/90 (53%)
Frame = +3
Query: 1319 RLSNFLIKNDFERGQVDTTLFRRTLKKDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEF 1378
+LS++L + + D +LF + ++ +YVDD+I + + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1379 EMSMMGELKFFLGIQINQSKEGVYVHQTKY 1408
+ +G LK+FLG+++ +S G+++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 35.0 bits (79), Expect(2) = 1e-05
Identities = 24/56 (42%), Positives = 34/56 (59%), Gaps = 2/56 (3%)
Frame = -2
Query: 1593 DNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDI--QFIDTEHQWADIFTK 1646
DN AA+ + K RAKHIEI HF+ D ++ LDI +F+++ Q D+FTK
Sbjct: 354 DNKAALHMPKI*F-SMRAKHIEIDFHFL*D--RRLYLDISTRFVNSNDQLTDVFTK 196
Score = 32.0 bits (71), Expect(2) = 1e-05
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = -1
Query: 1537 LGENLISWASKKQATIAMSTAEAEY 1561
L +NLISW SK+ +A S AEAE+
Sbjct: 517 LSDNLISWKSKETIIVARSRAEAEF 443
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.131 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,100,507
Number of Sequences: 28460
Number of extensions: 342275
Number of successful extensions: 1576
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 1533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1567
length of query: 1667
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1564
effective length of database: 1,966,220
effective search space: 3075168080
effective search space used: 3075168080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 62 (28.5 bits)
Medicago: description of AC147435.7