BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.5 + phase: 0
(789 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW719858 125 3e-29
TC19885 weakly similar to UP|ZPRO_MOUSE (Q9DA39) Z-protein (0610... 108 4e-24
TC16945 homologue to GB|CAA66613.1|1495271|ATRNAHELI RNA helicas... 40 0.002
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 37 0.016
BP071239 36 0.027
TC17687 weakly similar to GB|AAH05628.1|13542865|BC005628 acidic... 35 0.035
TC10734 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5g... 34 0.078
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 34 0.078
BI420041 34 0.10
AV779640 33 0.13
AV779190 32 0.51
BP075329 31 0.66
BP084441 31 0.66
TC14221 similar to GB|AAP37784.1|30725524|BT008425 At4g24690 {Ar... 30 1.1
BP057949 30 1.5
CB829198 30 1.5
TC15571 weakly similar to PIR|S19652|S19652 cellodextrinase C - ... 30 1.9
BP043430 30 1.9
AV415922 30 1.9
AI967639 29 2.5
>AW719858
Length = 543
Score = 125 bits (314), Expect = 3e-29
Identities = 69/159 (43%), Positives = 102/159 (63%)
Frame = +1
Query: 180 VVATNVAETSLTIPGIKYVVDTGREKVKNYDSSNGMETYEVKWISKASAAQRAGRAGRTA 239
+VATN+AETSLT+ GI YV+DTG K+K Y+ GM+ +V +S+A+A QRAGRAGRT
Sbjct: 4 IVATNIAETSLTVDGIYYVIDTGYGKMKVYNPRMGMDALQVFPVSRAAADQRAGRAGRTG 183
Query: 240 AGHCYRLYSSAAFSNEFPEFSPAEVEKVPVHGVVLLLKSMQIKKVANFPFPTSLKAASLL 299
G CYRLY+ +A+ NE E+++ + VVLLLKS++++ + +F F ++L
Sbjct: 184 PGTCYRLYTESAYLNEMLPSPVPEIQRTNLGNVVLLLKSLKVENLLDFDFMDPPPQDNIL 363
Query: 300 EAENCLRALEALDSKDELTLLGKAMALYPLSPRHSRMIL 338
+ L L AL++ LT LG M +PL P ++M+L
Sbjct: 364 NSMYQLWVLGALNNVGGLTDLGWKMVEFPLDPPLAKMLL 480
>TC19885 weakly similar to UP|ZPRO_MOUSE (Q9DA39) Z-protein (0610007H07Rik),
partial (9%)
Length = 501
Score = 108 bits (269), Expect = 4e-24
Identities = 53/72 (73%), Positives = 60/72 (82%)
Frame = +1
Query: 714 KLNSRLIDSSAMLRIVWKQNPRELFSEILDWFQQGFRKHFEELWLQMLGEVLQETQEQPL 773
KL SRLIDSSAMLR+ WK+NP ELFSEILDWFQ+ + K F+ LWL+ML EVLQETQEQPL
Sbjct: 1 KLKSRLIDSSAMLRMXWKENPGELFSEILDWFQKSYHKDFKLLWLKMLTEVLQETQEQPL 180
Query: 774 HKSLKRKSKVKS 785
+S KRK K KS
Sbjct: 181 RESFKRKFKGKS 216
>TC16945 homologue to GB|CAA66613.1|1495271|ATRNAHELI RNA helicase
{Arabidopsis thaliana;}, partial (15%)
Length = 610
Score = 39.7 bits (91), Expect = 0.002
Identities = 20/65 (30%), Positives = 37/65 (56%)
Frame = +1
Query: 550 YQSCMVDESIFIHRWSSVSTVHPEFLVYNELLETKRPNKEGETSAKRAYMHGVTNVDPTW 609
Y++ + ++ ++IH S++ P++++Y+EL+ T + YM VT +DP W
Sbjct: 211 YRTLVENQPVYIHPSSALFQRQPDWVIYHELVMT-----------TKEYMREVTVIDPKW 357
Query: 610 LVENA 614
LVE A
Sbjct: 358 LVELA 372
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 36.6 bits (83), Expect = 0.016
Identities = 28/87 (32%), Positives = 40/87 (45%), Gaps = 7/87 (8%)
Frame = +3
Query: 43 QTDRFSGYDEDDNNFDENESDSYDSETE---SELEFNDDDKNNHEGSKNNNNIVDVLGNE 99
QTD D+D+ DE+E D D + + E E +DDD EGS+ V+V G +
Sbjct: 240 QTDNEEEEDDDEEEDDEDEDDDDDDDDDDDGEEEEDDDDDDEEEEGSEE----VEVEGKD 407
Query: 100 G----SLASLKAAFENLSGQATLSSSN 122
G + + KAA G + S N
Sbjct: 408 GAKGAAAQTTKAAAAMCVGIGSFSDPN 488
>BP071239
Length = 429
Score = 35.8 bits (81), Expect = 0.027
Identities = 28/101 (27%), Positives = 42/101 (40%)
Frame = +3
Query: 168 VFDGVKEGERLVVVATNVAETSLTIPGIKYVVDTGREKVKNYDSSNGMETYEVKWISKAS 227
V + G V+VAT+VA L I I+ VV NYD G+E Y
Sbjct: 60 VLSQFRTGRSPVLVATDVAARGLDIKDIRVVV--------NYDFPTGVEDY-------VH 194
Query: 228 AAQRAGRAGRTAAGHCYRLYSSAAFSNEFPEFSPAEVEKVP 268
R GRAG T + + A ++++ + ++VP
Sbjct: 195 RIGRTGRAGATGLAYTFFAEQDAKYASDLIKVLEGANQRVP 317
>TC17687 weakly similar to GB|AAH05628.1|13542865|BC005628 acidic nuclear
phosphoprotein 32 family, member B {Mus musculus;},
partial (9%)
Length = 702
Score = 35.4 bits (80), Expect = 0.035
Identities = 14/38 (36%), Positives = 23/38 (59%)
Frame = +1
Query: 49 GYDEDDNNFDENESDSYDSETESELEFNDDDKNNHEGS 86
G +ED++N DE D Y+SE + E + + KN H+ +
Sbjct: 310 GVEEDEDNEDEEYDDDYESEYSEDEEDDQNSKNKHQAT 423
>TC10734 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (15%)
Length = 513
Score = 34.3 bits (77), Expect = 0.078
Identities = 26/102 (25%), Positives = 45/102 (43%), Gaps = 2/102 (1%)
Frame = +3
Query: 138 KIARENHDSSPG--ALFVLPLYAMLPAAAQLRVFDGVKEGERLVVVATNVAETSLTIPGI 195
++ ++H+ S L+ L L + L+ + G KEG+ ++VAT++ + I +
Sbjct: 216 RVLTQSHEYSEKRFTLYFLSLPMLYLPHTGLKRYKGFKEGQTRILVATDLVGRGIDIERV 395
Query: 196 KYVVDTGREKVKNYDSSNGMETYEVKWISKASAAQRAGRAGR 237
V+ NYD + +TY R GRAGR
Sbjct: 396 NIVI--------NYDMPDSADTY----------LHRVGRAGR 467
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 34.3 bits (77), Expect = 0.078
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +2
Query: 42 QQTDRFSGYDEDDNNFDENESDSYDSETESELEFNDDDKNNHEG 85
+Q+D F +EDD + DE+E D D + E E + +DDD+++ EG
Sbjct: 749 EQSD-FEDIEEDDEDGDEDE-DEDDDDDEEEEDDDDDDEDDEEG 874
>BI420041
Length = 452
Score = 33.9 bits (76), Expect = 0.10
Identities = 20/73 (27%), Positives = 39/73 (53%)
Frame = +2
Query: 10 NDSNVVNETSSVEGININEINEAFEMPGSSSMQQTDRFSGYDEDDNNFDENESDSYDSET 69
+DS V E + V+ ++++E ++ D F Y+E N+ DE+ + YD +
Sbjct: 197 SDSEPVLEPTIVQEVSMDE-------------EEDDEFD-YEEPLNDEDEDGDEYYDDDE 334
Query: 70 ESELEFNDDDKNN 82
+ E EF D+D+++
Sbjct: 335 QEEEEFEDEDEDS 373
>AV779640
Length = 428
Score = 33.5 bits (75), Expect = 0.13
Identities = 19/43 (44%), Positives = 27/43 (62%)
Frame = -2
Query: 305 LRALEALDSKDELTLLGKAMALYPLSPRHSRMILTVIKNTRYK 347
L AL AL+ ELT +G+ MA +PL P S+MI + + +YK
Sbjct: 352 LFALSALNKLGELTKVGRRMAEFPLDPMLSKMI---VASEKYK 233
>AV779190
Length = 539
Score = 31.6 bits (70), Expect = 0.51
Identities = 22/81 (27%), Positives = 38/81 (46%), Gaps = 15/81 (18%)
Frame = +3
Query: 26 INEINEAFEMPGSSSMQQTDRFSGYDEDDNNFDENE-----------SDSYDSETESEL- 73
+N +N A P SS+ D+ Y+ ++ +E +D DSE E E+
Sbjct: 198 VNGLNPA---PEPSSVDSDDQSDFYEYEEERAEEESRKNKRYDPVFVNDELDSEIEDEIV 368
Query: 74 ---EFNDDDKNNHEGSKNNNN 91
+ N+DD +++ G+K N N
Sbjct: 369 PSDDENEDDDDDYFGTKRNAN 431
>BP075329
Length = 466
Score = 31.2 bits (69), Expect = 0.66
Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +2
Query: 614 AKSSCIFSPPLTDPR--PFYDAQADQVKCWVIPTFGRFCWELPKHSIPISNVE 664
+++ + SPP T + P+ + CW F ++CW P HS+ I N++
Sbjct: 266 SETPLLLSPPPTMNQFSPYCHTSMSRTCCWY---FTQWCWSRPFHSLQIENMQ 415
>BP084441
Length = 344
Score = 31.2 bits (69), Expect = 0.66
Identities = 22/91 (24%), Positives = 41/91 (44%)
Frame = +2
Query: 556 DESIFIHRWSSVSTVHPEFLVYNELLETKRPNKEGETSAKRAYMHGVTNVDPTWLVENAK 615
D+ +F+ + HP FL+ ++L+ + KE + +A++ Y G + W +
Sbjct: 44 DKFLFLGIRIQLIQSHPIFLILLQVLDKAKSRKEQKMNAEKTYG*G*GLLSKVWCL---I 214
Query: 616 SSCIFSPPLTDPRPFYDAQADQVKCWVIPTF 646
S C++ T P D +V+ WV F
Sbjct: 215 SMCVY*QWYTLTYPVLDPLFXKVQXWVXRQF 307
>TC14221 similar to GB|AAP37784.1|30725524|BT008425 At4g24690 {Arabidopsis
thaliana;}, partial (12%)
Length = 827
Score = 30.4 bits (67), Expect = 1.1
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +3
Query: 42 QQTDRFSGYDEDDNNFDENESDSYDSETES--ELEFNDDDKNNHEGSKNNNNIVDVL 96
Q+ R +GY+ + D + +D E E+ F DDDKN KNN +I+ V+
Sbjct: 378 QEILRNNGYNLEQAIVDLCDESGWDPILEELKEMGFGDDDKNKKLLMKNNGSIMGVV 548
>BP057949
Length = 559
Score = 30.0 bits (66), Expect = 1.5
Identities = 30/103 (29%), Positives = 42/103 (40%)
Frame = -3
Query: 48 SGYDEDDNNFDENESDSYDSETESELEFNDDDKNNHEGSKNNNNIVDVLGNEGSLASLKA 107
SGY ED N+ S S +E + +F+ G + + ++V LG+
Sbjct: 458 SGYREDTNS--PRPSKSTRNEGRTSFDFSRSTSMKEGGRRYSGSMVMGLGSSN------- 306
Query: 108 AFENLSGQATLSSSNVNTEDSLDQSKVGREKIARENHDSSPGA 150
NL + +LS S LD K EK A DSS GA
Sbjct: 305 ---NLRNEGSLSYS-------LDTEKSDIEKFAEALKDSSVGA 207
>CB829198
Length = 562
Score = 30.0 bits (66), Expect = 1.5
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = +3
Query: 370 NPFVMQYEGNDSNKDSETSEKSRMGDNENNIDKTEK-TKRKKLKQTS 415
N V + + +DS +++E E+ GDNENN K K K+KK K+ S
Sbjct: 267 NDDVEESQHSDSKEETE-EEEDEDGDNENNKPKRAKAAKKKKNKERS 404
>TC15571 weakly similar to PIR|S19652|S19652 cellodextrinase C - Pseudomonas
fluorescens {Pseudomonas fluorescens;} , partial (6%)
Length = 784
Score = 29.6 bits (65), Expect = 1.9
Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 4/44 (9%)
Frame = -2
Query: 40 SMQQTDRFSGYDEDDNNFDENESDSYDS----ETESELEFNDDD 79
S ++TD D+ D++ DE E+DS+D ET+S L +D++
Sbjct: 783 SEEETDS----DDQDDDDDEEETDSFDQGDEEETDSSLSHSDEE 664
>BP043430
Length = 493
Score = 29.6 bits (65), Expect = 1.9
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -1
Query: 49 GYDEDDNNFDENESDSYDSETESELE-FNDDDKNN 82
G D + + DE E DS D + SE E FNDD++++
Sbjct: 340 GNDNEVDEEDEEEEDSNDDDDGSESEEFNDDEEDS 236
Score = 28.5 bits (62), Expect = 4.3
Identities = 22/62 (35%), Positives = 29/62 (46%)
Frame = -1
Query: 48 SGYDEDDNNFDENESDSYDSETESELEFNDDDKNNHEGSKNNNNIVDVLGNEGSLASLKA 107
SG D DD D NE D D E E ++DD + E + N++ D + L SLK
Sbjct: 364 SGKDNDDEGND-NEVDEEDEEEED----SNDDDDGSESEEFNDDEEDSD*TQQILWSLKG 200
Query: 108 AF 109
F
Sbjct: 199 EF 194
>AV415922
Length = 422
Score = 29.6 bits (65), Expect = 1.9
Identities = 13/32 (40%), Positives = 19/32 (58%), Gaps = 3/32 (9%)
Frame = +1
Query: 63 DSYDSETESE---LEFNDDDKNNHEGSKNNNN 91
D DS+T+ + N+DD NN+ + NNNN
Sbjct: 127 DGQDSDTQFNSKGINTNNDDNNNNNNNNNNNN 222
>AI967639
Length = 335
Score = 29.3 bits (64), Expect = 2.5
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +1
Query: 42 QQTDRFSGYDEDDNNFDENESDSYDSETESELEFNDDD 79
Q+ D S + D+ + +E++ D Y+ E E E + +DDD
Sbjct: 70 QRRDISSSSEGDEEDEEEDQGDEYNLEDEEEDDDDDDD 183
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.130 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,862,879
Number of Sequences: 28460
Number of extensions: 176943
Number of successful extensions: 977
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 959
length of query: 789
length of database: 4,897,600
effective HSP length: 98
effective length of query: 691
effective length of database: 2,108,520
effective search space: 1456987320
effective search space used: 1456987320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC147012.5