BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.12 + phase: 0
(750 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP054035 151 4e-37
TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-l... 28 0.18
AV410494 32 0.49
TC10498 31 0.63
TC9527 similar to GB|AAB04607.1|1448917|ATHTHSY threonine syntha... 30 1.1
TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, part... 30 1.8
TC13079 weakly similar to UP|Q9LJI7 (Q9LJI7) Protein kinase, par... 30 1.8
TC14636 weakly similar to UP|Q9ZUS6 (Q9ZUS6) At2g37400 protein, ... 29 2.4
BI420337 26 2.9
AV422938 29 3.1
AV407242 28 4.1
TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 28 4.1
TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (... 28 4.1
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 28 5.4
AV423824 28 5.4
TC16843 homologue to UP|Q9ZP04 (Q9ZP04) Alpha-D-xylosidase precu... 28 5.4
BI418438 28 5.4
AV422745 28 7.0
TC19908 similar to GB|CAA60022.1|791150|VURNEXT26 extensin-like ... 28 7.0
AV417475 27 9.2
>BP054035
Length = 498
Score = 151 bits (381), Expect = 4e-37
Identities = 71/82 (86%), Positives = 79/82 (95%)
Frame = -2
Query: 669 EITDVHRKVHMYVDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALL 728
EI+D+HRKVHMYVDSREKVTAR+QLTCPS+RE++VFGSF+ TGTLLPKAPKGWGWR+ALL
Sbjct: 497 EISDIHRKVHMYVDSREKVTARFQLTCPSQREIMVFGSFQPTGTLLPKAPKGWGWRSALL 318
Query: 729 FDELADLLQNGALRVIAVVQLV 750
FDELADLL NG LRVIAVVQLV
Sbjct: 317 FDELADLLPNGVLRVIAVVQLV 252
>TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-like,
partial (32%)
Length = 706
Score = 27.7 bits (60), Expect(2) = 0.18
Identities = 25/76 (32%), Positives = 35/76 (45%), Gaps = 3/76 (3%)
Frame = +2
Query: 4 PQYPNNPPPSPYNQRSASIPKQPPSHHSDNND-PTTAELRALDCNLASLCEHVQIEGF-- 60
P YP++PPP + +P P S S PT +LR + SL V+ GF
Sbjct: 314 PPYPSSPPP----HVAWKLPSSPCSLSSPTTPRPTRKDLRNVPFACRSL-NRVKRVGFCP 478
Query: 61 NSGSFSDIVVNAMGST 76
N+ + S +V GST
Sbjct: 479 NASTLSTPIVLTCGST 526
Score = 23.9 bits (50), Expect(2) = 0.18
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 108 VEHPAHGIPGSESGTGIHSDNTKGKGIA 135
V+ P HG S SG+G+ +DN G ++
Sbjct: 610 VDVPEHG---SSSGSGLETDNRTGPEVS 684
>AV410494
Length = 398
Score = 31.6 bits (70), Expect = 0.49
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +2
Query: 303 PRGNYRDEDDVFINIFEGGSLLYCN 327
PR RDE+DV F+GGSL+ C+
Sbjct: 119 PRSQKRDEEDVCFICFDGGSLVLCD 193
>TC10498
Length = 818
Score = 31.2 bits (69), Expect = 0.63
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +1
Query: 3 NPQYPNNPPPSPYNQRSASIPK-QPPSHHS 31
NPQ+P N P++Q S+ + PP+HHS
Sbjct: 13 NPQFPGNTLRPPFHQSSSGLTGFDPPAHHS 102
>TC9527 similar to GB|AAB04607.1|1448917|ATHTHSY threonine synthase
{Arabidopsis thaliana;} , partial (23%)
Length = 544
Score = 30.4 bits (67), Expect = 1.1
Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 4/39 (10%)
Frame = -1
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSH----HSDNNDPT 37
+P P +PP SP + ++S+P +PP + S +N PT
Sbjct: 235 SPPPPYHPPQSPRSFSASSVPSKPPQYAGK*TSQSNPPT 119
>TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, partial (5%)
Length = 492
Score = 29.6 bits (65), Expect = 1.8
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +3
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTT 38
N P+NPPPSP S S PK +S N+P T
Sbjct: 93 NVHPPSNPPPSP----STSNPKTKTRWNSTTNNPLT 188
>TC13079 weakly similar to UP|Q9LJI7 (Q9LJI7) Protein kinase, partial (7%)
Length = 607
Score = 29.6 bits (65), Expect = 1.8
Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 8/54 (14%)
Frame = -2
Query: 3 NPQY-PNNPPPSPYNQRSASI-----PKQPPS--HHSDNNDPTTAELRALDCNL 48
N Q+ P PPP P + S+S+ PK PP HHS + P+ A R + N+
Sbjct: 402 NTQFLPLPPPPQPPSSFSSSLLIYSHPKTPPPP*HHSQHPTPSLARPREPNPNI 241
>TC14636 weakly similar to UP|Q9ZUS6 (Q9ZUS6) At2g37400 protein, partial
(43%)
Length = 1316
Score = 29.3 bits (64), Expect = 2.4
Identities = 18/44 (40%), Positives = 24/44 (53%), Gaps = 7/44 (15%)
Frame = +3
Query: 8 NNPPPSPYNQ------RSASIPKQPPSH-HSDNNDPTTAELRAL 44
+NPP SP +Q S+S P +PP H + NN T LR+L
Sbjct: 249 SNPPTSP*SQPHSSSCASSSSPPRPPPHSYLSNNPADTEALRSL 380
>BI420337
Length = 397
Score = 26.2 bits (56), Expect(2) = 2.9
Identities = 13/34 (38%), Positives = 15/34 (43%), Gaps = 3/34 (8%)
Frame = +1
Query: 6 YPNNPPPSPYNQRSASIPKQ---PPSHHSDNNDP 36
Y + PPP PY S P PP H+S P
Sbjct: 97 YTSPPPPKPYYYHSPPPPMHSPPPPYHYSSPPPP 198
Score = 21.2 bits (43), Expect(2) = 2.9
Identities = 10/27 (37%), Positives = 11/27 (40%)
Frame = +2
Query: 29 HHSDNNDPTTAELRALDCNLASLCEHV 55
HH T+ L L C SL HV
Sbjct: 188 HHHHQRSHTSTLLPHLQCTSTSLHHHV 268
>AV422938
Length = 466
Score = 28.9 bits (63), Expect = 3.1
Identities = 21/75 (28%), Positives = 32/75 (42%), Gaps = 4/75 (5%)
Frame = -2
Query: 134 IADGCTNKMLETDLGKMSLKSDLKDTS----MPNLLVELGDSEGDFNSDICDSNQRVQLA 189
+ADGC +K +E D + L + S P+ + +L DSEG D N +A
Sbjct: 237 VADGCADKQVEDDPSEFPLADQSIEHSQLMKQPSQVAQLEDSEG------MD*NHMSSVA 76
Query: 190 SYDISPNLNPSYPSD 204
Y P + + D
Sbjct: 75 HYHQPP*MGSGHALD 31
>AV407242
Length = 320
Score = 28.5 bits (62), Expect = 4.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 8 NNPPPSPYNQRSASIPKQPPSHH 30
N+ PPSP + S ++ PP HH
Sbjct: 104 NHRPPSPSSTNSRTVSSTPPHHH 172
>TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (10%)
Length = 309
Score = 28.5 bits (62), Expect = 4.1
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPP 27
+P PN+PPP P + P +PP
Sbjct: 157 SPSLPNSPPPPPRSGHHHPSPSEPP 231
>TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (44%)
Length = 848
Score = 28.5 bits (62), Expect = 4.1
Identities = 13/32 (40%), Positives = 15/32 (46%)
Frame = +2
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNN 34
N YP PPP P+ + P PP SD N
Sbjct: 455 NQGYPMQPPPQPFYMQ----PHPPPQMFSDEN 538
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 28.1 bits (61), Expect = 5.4
Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 3/39 (7%)
Frame = +1
Query: 3 NPQYPNNPPPSPY---NQRSASIPKQPPSHHSDNNDPTT 38
+P P++ SP+ + S++ P PPSH ++NN+ +T
Sbjct: 151 SPSSPSSSSLSPWLSPSPPSSTTPPPPPSHRTNNNNLST 267
>AV423824
Length = 181
Score = 28.1 bits (61), Expect = 5.4
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = +1
Query: 10 PPPSPYNQRSASIPKQPPSHH 30
PP P+ +R + +P++PP H
Sbjct: 79 PPAQPHRRRRSRLPRRPPQEH 141
>TC16843 homologue to UP|Q9ZP04 (Q9ZP04) Alpha-D-xylosidase precursor,
partial (27%)
Length = 787
Score = 28.1 bits (61), Expect = 5.4
Identities = 26/98 (26%), Positives = 38/98 (38%), Gaps = 2/98 (2%)
Frame = -3
Query: 536 GASTGGIPVQLLETPEHTIGIEWENDNSSSISLDLKTPLS--HFPPFRFGVSFEEVHRLG 593
G S+ + V L++ +HT + + L PL HFP +F SF +HR
Sbjct: 362 GGSSHRVLVMFLQSRQHT--------HPGPVPLGHTFPLGILHFPEQKFEASFISIHRPS 207
Query: 594 DGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTLGNV 631
G S WK I + + E G R G +
Sbjct: 206 TGT-----------SSWKRRISSPHQETVFGLRKSGKL 126
>BI418438
Length = 431
Score = 28.1 bits (61), Expect = 5.4
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +1
Query: 7 PNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAEL 41
P P P+P ++ +S P +PP+ S+ N P+ L
Sbjct: 244 PPPPSPTPPPRQKSSKPARPPASPSNANPPSPTSL 348
>AV422745
Length = 440
Score = 27.7 bits (60), Expect = 7.0
Identities = 20/87 (22%), Positives = 37/87 (41%)
Frame = +1
Query: 188 LASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTGVGIEGPSEEG 247
LA ++P + D +G + + L DGV TS V G T + ++ ++
Sbjct: 151 LARLGLNPRVITKIADDSQGRGILDELQ-ADGVDTSFIVVSKEGTSPFTYIIVDNETKTR 327
Query: 248 SCYHSDNNNRLVRDQSRDSFSSSSCSG 274
+C H+ ++ D +S S+ G
Sbjct: 328 TCIHTPGYPPMIPDDLPESSLLSALDG 408
>TC19908 similar to GB|CAA60022.1|791150|VURNEXT26 extensin-like protein
{Vigna unguiculata;} , partial (49%)
Length = 472
Score = 27.7 bits (60), Expect = 7.0
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = +2
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHHS 31
++P P++ PP PY +S P HHS
Sbjct: 122 KSPPPPSHSPPPPYVYKSPPPPSSSHPHHS 211
>AV417475
Length = 198
Score = 27.3 bits (59), Expect = 9.2
Identities = 11/17 (64%), Positives = 12/17 (69%)
Frame = +2
Query: 7 PNNPPPSPYNQRSASIP 23
P +PPP P N RSAS P
Sbjct: 116 PPSPPPPPANGRSASFP 166
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,223,174
Number of Sequences: 28460
Number of extensions: 222354
Number of successful extensions: 1804
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1673
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1783
length of query: 750
length of database: 4,897,600
effective HSP length: 97
effective length of query: 653
effective length of database: 2,136,980
effective search space: 1395447940
effective search space used: 1395447940
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC147012.12