BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147011.8 - phase: 0
(226 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14830 similar to UP|Q8GVF6 (Q8GVF6) Pyrrolidone carboxyl pepti... 387 e-108
AV774990 89 3e-40
TC13480 weakly similar to UP|Q9AA65 (Q9AA65) Elongation factor T... 27 2.9
CN825308 27 3.8
TC14482 similar to UP|PSBY_ARATH (O49347) Photosystem II core co... 26 6.4
TC13845 weakly similar to GB|AAM98310.1|22655436|AY142046 At2g19... 25 8.4
>TC14830 similar to UP|Q8GVF6 (Q8GVF6) Pyrrolidone carboxyl peptidase-like
protein, partial (91%)
Length = 1088
Score = 387 bits (993), Expect = e-108
Identities = 193/222 (86%), Positives = 208/222 (92%), Gaps = 2/222 (0%)
Frame = +3
Query: 1 MGSEGPSAA-VTTVYITGFKKFHGVSENPTETIVNNLTEYVKKKGLPKGLAIGSCSILDT 59
MGSEGP+AA VTTV++TGFKKFHGVSENPTE IV NLTEY+ KKGLPKGL IGSC+IL+T
Sbjct: 153 MGSEGPAAAAVTTVHVTGFKKFHGVSENPTEAIVRNLTEYMNKKGLPKGLVIGSCTILET 332
Query: 60 AGQGALVPLYQTLQSAIIA-KESESSSSNKIIWLHFGVNSGATRFAIERQAVNEATFRCP 118
AGQGALVPLYQTLQSAI A KESESSSSN++IWLHFGVNSGAT+FAIERQAVNEATFRCP
Sbjct: 333 AGQGALVPLYQTLQSAITAAKESESSSSNRVIWLHFGVNSGATKFAIERQAVNEATFRCP 512
Query: 119 DEMGWKPQKVPIVPSDGPISRIRETTLPVEEITKTLATKGYDVMTSDDAGRFVCNYVYYH 178
DEMGWKPQKVPIVPSDG ISR RETTLPVEEITK LA KGY+VMTSDDAGRFVCNYVYYH
Sbjct: 513 DEMGWKPQKVPIVPSDGAISRTRETTLPVEEITKALAKKGYNVMTSDDAGRFVCNYVYYH 692
Query: 179 SLRFAEQNGNKSLFVHVPLFFTINEETQMQFAASLLEVLANV 220
SLRFAEQNG KSLFVHVPLFFTI+EE QMQFAASLL++L ++
Sbjct: 693 SLRFAEQNGIKSLFVHVPLFFTIDEEVQMQFAASLLDLLPSI 818
>AV774990
Length = 488
Score = 89.0 bits (219), Expect(3) = 3e-40
Identities = 42/54 (77%), Positives = 46/54 (84%)
Frame = -2
Query: 105 IERQAVNEATFRCPDEMGWKPQKVPIVPSDGPISRIRETTLPVEEITKTLATKG 158
IERQAV EATF CPDEMGWKPQ+VP+VPSDG ISR RETTLPVEEIT+ +G
Sbjct: 487 IERQAVYEATFPCPDEMGWKPQQVPLVPSDGAISRTRETTLPVEEITQAPGQEG 326
Score = 59.7 bits (143), Expect(3) = 3e-40
Identities = 29/42 (69%), Positives = 35/42 (83%)
Frame = -1
Query: 179 SLRFAEQNGNKSLFVHVPLFFTINEETQMQFAASLLEVLANV 220
SLRFAE NG +SLFVHVPLFFTI+E M FAASLL++L ++
Sbjct: 263 SLRFAEPNGIQSLFVHVPLFFTIDEAVPMPFAASLLDLLPSI 138
Score = 53.1 bits (126), Expect(3) = 3e-40
Identities = 22/29 (75%), Positives = 26/29 (88%)
Frame = -3
Query: 150 ITKTLATKGYDVMTSDDAGRFVCNYVYYH 178
+ + LA KGY+VMTSDDAGRFVC+YVYYH
Sbjct: 351 LPRPLAKKGYNVMTSDDAGRFVCHYVYYH 265
>TC13480 weakly similar to UP|Q9AA65 (Q9AA65) Elongation factor Tu family
protein, partial (21%)
Length = 607
Score = 26.9 bits (58), Expect = 2.9
Identities = 19/65 (29%), Positives = 25/65 (38%)
Frame = -3
Query: 129 PIVPSDGPISRIRETTLPVEEITKTLATKGYDVMTSDDAGRFVCNYVYYHSLRFAEQNGN 188
P VP GP+S I ITK + ++TS F V Y +L N N
Sbjct: 398 PTVPDTGPMSVISARL*DKASITKPTCSSFTSIVTSSTGSSF*PFSVLYMTLGGETDNSN 219
Query: 189 KSLFV 193
S +
Sbjct: 218 PSRLI 204
>CN825308
Length = 600
Score = 26.6 bits (57), Expect = 3.8
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +2
Query: 46 PKGLAIGSCSILDTAGQGALVPLYQTLQSAIIAKESESSSS 86
PK LAI S+ T G A PLY + + KE S+ S
Sbjct: 62 PKSLAISLDSMASTEGSAAPPPLYFDEKWKLSKKEGSSTRS 184
>TC14482 similar to UP|PSBY_ARATH (O49347) Photosystem II core complex
proteins psbY, chloroplast precursor (L-arginine
metabolising enzyme) (L-AME) [Contains: Photosystem II
protein psbY-1 (psbY-A1); Photosystem II protein psbY-2
(psbY-A2)], partial (55%)
Length = 1006
Score = 25.8 bits (55), Expect = 6.4
Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 7/87 (8%)
Frame = +1
Query: 1 MGSEGPSAAVTTVYITGFKKFHG-VSENPTETIVNNLTEYVKKKGLPKGL------AIGS 53
+GS+ + TT+ + K + + NPT T + + + LPKGL +I +
Sbjct: 82 IGSQMAATIATTMAMINSKCLNTKLMINPTNTKLTTSKPTISFQNLPKGLSSAENTSIST 261
Query: 54 CSILDTAGQGALVPLYQTLQSAIIAKE 80
S+ TA GA+ + +A A++
Sbjct: 262 TSMAGTAIAGAIFSSLVSCDAAFAAQQ 342
>TC13845 weakly similar to GB|AAM98310.1|22655436|AY142046
At2g19080/T20K24.9 {Arabidopsis thaliana;}, partial
(39%)
Length = 485
Score = 25.4 bits (54), Expect = 8.4
Identities = 8/31 (25%), Positives = 18/31 (57%)
Frame = +1
Query: 117 CPDEMGWKPQKVPIVPSDGPISRIRETTLPV 147
C + + W P +P +P + ++R ++LP+
Sbjct: 55 CEEALFWSPNWLPSMPLRFHLPQVRSSSLPI 147
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,531,273
Number of Sequences: 28460
Number of extensions: 43851
Number of successful extensions: 191
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of query: 226
length of database: 4,897,600
effective HSP length: 87
effective length of query: 139
effective length of database: 2,421,580
effective search space: 336599620
effective search space used: 336599620
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147011.8