BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147010.10 - phase: 1
(150 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacety... 35 0.004
TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinog... 32 0.036
TC15487 similar to UP|O23144 (O23144) Proton pump interactor (AT... 32 0.061
TC14012 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 ... 31 0.10
TC9400 UP|TX20_HUMAN (Q9UMR3) T-box transcription factor TBX20 (... 31 0.10
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 30 0.14
TC17463 similar to UP|AAR15430 (AAR15430) Autophagocytosis prote... 30 0.23
BP034914 29 0.39
BP065600 29 0.39
TC15683 weakly similar to UP|Q8GTM1 (Q8GTM1) WD40, partial (4%) 28 0.67
TC8662 similar to UP|RK11_ARATH (Q9MAP3) 50S ribosomal protein L... 28 0.67
TC13772 28 0.67
AV409475 28 0.88
BP053165 27 1.1
AI967840 27 1.1
TC15875 similar to UP|PS21_ARATH (O23714) Proteasome subunit bet... 27 1.1
BI417283 27 1.1
TC18555 similar to GB|AAP68307.1|31711902|BT008868 At2g33570 {Ar... 27 1.1
TC10582 27 1.5
BP065061 26 2.5
>TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacetylase 1
(HD1), partial (5%)
Length = 636
Score = 35.4 bits (80), Expect = 0.004
Identities = 17/72 (23%), Positives = 36/72 (49%)
Frame = +3
Query: 62 GSAQRKEKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKSVKNAKNTKQQRQA 121
G A+ EKK EPK+ ++ K K + + +K+ A+ + K + K+ K++ +
Sbjct: 12 GPAKEPEKKEEPKKEEPQKEEEK---KDEPKSEEPQKEEAKKEEPKKEEEKKDEKKEEEK 182
Query: 122 KSHRDREVTAVV 133
K + ++ V+
Sbjct: 183 KKEQPAQIDPVL 218
>TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinogalactan
protein 1 precursor, partial (43%)
Length = 890
Score = 32.3 bits (72), Expect = 0.036
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 4/81 (4%)
Frame = +3
Query: 44 PEVKNSSTDHGGRRRRQTGSAQRKEKKTEPKQHASKQHASKRARKQRARKQRA----RKQ 99
P ++ TDH R RRQ R KT P H ++H + R+Q A ++
Sbjct: 300 PRRRDQPTDHNHRLRRQQRRHGRPPLKT-PINHHRQEHPLPPRPPRLLRRQEAPPDHQRH 476
Query: 100 RARNKNVKSVKNAKNTKQQRQ 120
R R NV S ++ ++ RQ
Sbjct: 477 RPRRHNVPSHRHRPGLRRIRQ 539
>TC15487 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (16%)
Length = 1153
Score = 31.6 bits (70), Expect = 0.061
Identities = 15/54 (27%), Positives = 32/54 (58%), Gaps = 1/54 (1%)
Frame = +3
Query: 56 RRRRQTGSAQRK-EKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKS 108
++R + G +R ++K +PKQ + + +R+ + R+QR + + RN+ +KS
Sbjct: 471 QKRSRPGKGRRS*QRKRQPKQLSKHKRKLRRSLRTARRRQRRKLELWRNRQMKS 632
>TC14012 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 , partial
(13%)
Length = 480
Score = 30.8 bits (68), Expect = 0.10
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 1/65 (1%)
Frame = -2
Query: 39 SANSSPEVKNSSTDHGGRRRRQTGSAQRKEKKTEPKQHASKQHASKRA-RKQRARKQRAR 97
S + + E + + HG RRR +A+R ++ A K S A RK A K+R R
Sbjct: 263 SRSPNAEPPSPARSHGCSRRRPRCTARRGSERRRGSNRACKDPPS*NANRKNLAGKKRRR 84
Query: 98 KQRAR 102
++R R
Sbjct: 83 RRRRR 69
>TC9400 UP|TX20_HUMAN (Q9UMR3) T-box transcription factor TBX20 (T-box
protein 20) (Fragment), partial (5%)
Length = 543
Score = 30.8 bits (68), Expect = 0.10
Identities = 20/72 (27%), Positives = 38/72 (52%)
Frame = -3
Query: 57 RRRQTGSAQRKEKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKSVKNAKNTK 116
RRR+ + + E ++ A + A +R +++R RKQ RK+ RN++ K K +
Sbjct: 298 RRRRR*IPSKNQSHHERRRKA--EIADQRRKRERRRKQGNRKRLIRNRDNKEGKTRPTSF 125
Query: 117 QQRQAKSHRDRE 128
R+ K ++R+
Sbjct: 124 IGRKNKRKKERK 89
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 30.4 bits (67), Expect = 0.14
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 14/94 (14%)
Frame = +2
Query: 41 NSSPEVKNSSTDHGGRRRRQTGSAQRKEKKTEPKQHA-----------SKQHASKRARKQ 89
N P+ + R+++Q Q +K PK H S + S++ RKQ
Sbjct: 251 NQPPKNQRKKPQKNQRKQKQKQKPQLLQK---PKTHLKLRTRR*QRKPSLRK*SQKQRKQ 421
Query: 90 RARKQRARKQRARNKNVKSVKN---AKNTKQQRQ 120
R +KQR++KQR+ + +K + K T QQR+
Sbjct: 422 R-KKQRSQKQRSLQQLLKQSQQHL*RKRTNQQRK 520
>TC17463 similar to UP|AAR15430 (AAR15430) Autophagocytosis protein, partial
(45%)
Length = 543
Score = 29.6 bits (65), Expect = 0.23
Identities = 14/54 (25%), Positives = 25/54 (45%)
Frame = +3
Query: 51 TDHGGRRRRQTGSAQRKEKKTEPKQHASKQHASKRARKQRARKQRARKQRARNK 104
++ GGRRRR+ A + ++ + + R R QR R + + R R +
Sbjct: 30 SEFGGRRRRRRDGADSEASRSFQRHRRENHRSPHRLRLQRERSSQCLRVRHRRR 191
>BP034914
Length = 544
Score = 28.9 bits (63), Expect = 0.39
Identities = 21/86 (24%), Positives = 39/86 (44%), Gaps = 8/86 (9%)
Frame = +3
Query: 28 LHHRIDSTAIQSANSSPEVKNSSTDHGGRRRRQT--------GSAQRKEKKTEPKQHASK 79
+H S + S++S+ +S +D RRRR+ GS + +E
Sbjct: 51 VHDDTPSPSSSSSSSTASDSDSDSDRSSRRRREKERRRRSDGGSKRERE----------- 197
Query: 80 QHASKRARKQRARKQRARKQRARNKN 105
S R R++R ++ R RK+ R+++
Sbjct: 198 ---SSRRREKRKKRDRDRKKTKRHRH 266
>BP065600
Length = 486
Score = 28.9 bits (63), Expect = 0.39
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +1
Query: 74 KQHASKQHASKRARKQRARKQRARKQRARN 103
+QHAS H+S QR + + +R+++ RN
Sbjct: 166 RQHASSHHSSHEINTQRKKGKHSREKKIRN 255
>TC15683 weakly similar to UP|Q8GTM1 (Q8GTM1) WD40, partial (4%)
Length = 957
Score = 28.1 bits (61), Expect = 0.67
Identities = 15/49 (30%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Frame = +3
Query: 57 RRRQTGSAQRKEKKTEPKQHASKQHASKRARKQRARKQ-RARKQRARNK 104
+ R+ S +KEK + ++ +QH KR Q RK+ RA+ + + K
Sbjct: 552 KERKDLSRVKKEKSLKEMKNMRRQHQEKREASQHPRKETRAQNAKLQTK 698
>TC8662 similar to UP|RK11_ARATH (Q9MAP3) 50S ribosomal protein L11,
chloroplast precursor (CL11), partial (72%)
Length = 1261
Score = 28.1 bits (61), Expect = 0.67
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = -1
Query: 10 KNQKTENSSEILRDSCTNLHHRIDSTAIQ 38
K+++ +S I R +C N H R +S AIQ
Sbjct: 733 KDRRININSHICRSACYNSHRRFESRAIQ 647
>TC13772
Length = 409
Score = 28.1 bits (61), Expect = 0.67
Identities = 15/52 (28%), Positives = 23/52 (43%)
Frame = -3
Query: 67 KEKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKSVKNAKNTKQQ 118
+EKKT K+ KRA + A RK ++R K N + K++
Sbjct: 338 QEKKTNRKK*TESYRGQKRAPRHTAAATLGRKTQSRKATSKHNSNTSSAKRE 183
>AV409475
Length = 335
Score = 27.7 bits (60), Expect = 0.88
Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 4/76 (5%)
Frame = +2
Query: 55 GRRRRQTGSAQRKEKKTEPK---QHASKQHASKRARKQRARKQRARKQRARNKNVKSVKN 111
GRRRR + +RKE+ P+ ++ SK+ K + Q+ K A + K +K
Sbjct: 86 GRRRRASSMGKRKERIQNPEPFVPDGAEYAKSKKPSKAPKQHQKEEKMIASCISSKIMKE 265
Query: 112 AK-NTKQQRQAKSHRD 126
A K++ + + H +
Sbjct: 266 ALIQQKEEAEEEDHNN 313
>BP053165
Length = 559
Score = 27.3 bits (59), Expect = 1.1
Identities = 17/60 (28%), Positives = 27/60 (44%), Gaps = 5/60 (8%)
Frame = +1
Query: 73 PKQHASKQHASKRA-----RKQRARKQRARKQRARNKNVKSVKNAKNTKQQRQAKSHRDR 127
PK+H SKQH S + +K A++ R N V + + A + Q+ S + R
Sbjct: 259 PKKHPSKQHRSAQGST*PLQKYTAKQSPWRSSSTMNNAVHTGQGAASYNPQQHISSTKQR 438
>AI967840
Length = 393
Score = 27.3 bits (59), Expect = 1.1
Identities = 19/81 (23%), Positives = 37/81 (45%)
Frame = +1
Query: 68 EKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKSVKNAKNTKQQRQAKSHRDR 127
EKK K+ A+++ +A K+ K A + + K KSV+ K + + H D
Sbjct: 64 EKKPAEKKPAAEKAPPAKAEKKVPTKDAASVDKKKKKKSKSVETYKIYIFKVLKQVHPDI 243
Query: 128 EVTAVVLPLSPSVFSSFFVKV 148
+++ + + S + F K+
Sbjct: 244 GISSKAMGIMNSFINDIFEKL 306
>TC15875 similar to UP|PS21_ARATH (O23714) Proteasome subunit beta type 2-1
(20S proteasome alpha subunit D1) , partial (97%)
Length = 992
Score = 27.3 bits (59), Expect = 1.1
Identities = 14/51 (27%), Positives = 25/51 (48%)
Frame = -2
Query: 39 SANSSPEVKNSSTDHGGRRRRQTGSAQRKEKKTEPKQHASKQHASKRARKQ 89
++N +P + DH +R T +RKEK T K +K+ + + +Q
Sbjct: 826 NSNDTPLTFRALRDHDFQRHPNTNKRKRKEKTTGKKSIQNKEQS*LKLEEQ 674
>BI417283
Length = 557
Score = 27.3 bits (59), Expect = 1.1
Identities = 14/37 (37%), Positives = 23/37 (61%)
Frame = -1
Query: 54 GGRRRRQTGSAQRKEKKTEPKQHASKQHASKRARKQR 90
GGRRRR+ Q KE+K + K+ ++ A ++ K+R
Sbjct: 119 GGRRRRRCYQEQEKEQKWK-KEETEEE*ARRKRMKER 12
>TC18555 similar to GB|AAP68307.1|31711902|BT008868 At2g33570 {Arabidopsis
thaliana;}, partial (19%)
Length = 759
Score = 27.3 bits (59), Expect = 1.1
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +3
Query: 29 HHRIDSTAIQSANSSPEVKNSSTDHGGRRRRQTGSAQRKEKKTEPKQHASKQHASKRARK 88
HHR + + S+ + + GGRR QT + ++ + QH Q ++ R
Sbjct: 279 HHRNLTASSCSSTAVTTTTGRARASGGRRCHQTNLQPVRLRRLQLHQHGRLQRRTQHVRH 458
Query: 89 QRARKQRA 96
R R + A
Sbjct: 459 GRPRFEAA 482
>TC10582
Length = 382
Score = 26.9 bits (58), Expect = 1.5
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
Frame = -2
Query: 34 STAIQSANSSPEVKNSSTDHGGRRRRQTGSAQRKEKKTEP--KQHASKQHASKRARKQRA 91
S A ++ANS E+K T+HG R +A K +TEP +++ + + K ++
Sbjct: 267 SKARKTANSKNEIKA*RTEHGA-RGGSLEAANTKAVETEPGGRKYRTWEENGKEGEQRT* 91
Query: 92 RKQRARKQRA 101
K + R RA
Sbjct: 90 GKTKGRFIRA 61
>BP065061
Length = 302
Score = 26.2 bits (56), Expect = 2.5
Identities = 13/47 (27%), Positives = 25/47 (52%)
Frame = +3
Query: 63 SAQRKEKKTEPKQHASKQHASKRARKQRARKQRARKQRARNKNVKSV 109
S R+E+ +H KQ ++ R++ K++ +KQR + K+V
Sbjct: 45 SKNRQEQLHYK*EHKLKQXKKEKERQRSQXKRQQQKQRGCTREAKTV 185
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.308 0.118 0.310
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,976,468
Number of Sequences: 28460
Number of extensions: 19400
Number of successful extensions: 258
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of query: 150
length of database: 4,897,600
effective HSP length: 82
effective length of query: 68
effective length of database: 2,563,880
effective search space: 174343840
effective search space used: 174343840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC147010.10